目的:探讨重症监护病房(intensive care unit,ICU)患者铜绿假单胞菌IMP-1耐药基因检测及耐药性,为临床合理使用抗生素提供依据。方法:采用聚合酶链反应(PCR)检测耐药基因IMP-1,ATB Expression自动鉴定病原菌及药敏分析。结果:2007～200...目的:探讨重症监护病房(intensive care unit,ICU)患者铜绿假单胞菌IMP-1耐药基因检测及耐药性,为临床合理使用抗生素提供依据。方法:采用聚合酶链反应(PCR)检测耐药基因IMP-1,ATB Expression自动鉴定病原菌及药敏分析。结果:2007～2009年共分离出106株铜绿假单胞菌,对头孢他啶、亚胺培南、哌拉西林、氨曲南、头孢哌酮、头孢吡肟、左氧氟沙星的耐药率分别为50.9%、39.6%、79.2%、56.6%、57.5%、41.5%、25.5%。每年耐亚胺培南株的检出率依序分别为16.0%、28.3%、39.6%。耐亚胺培南株绝大部分检出IMP耐药基因。结论:ICU患者金属酶表型阳性菌株检出的基因型主要为IMP-1,产酶菌株耐药性强,随时进行细菌耐药性监测,正确使用有效的抗生素是控制感染和延缓细菌耐药的关键。展开更多
As a zinc-dependent enzyme, metal-β-lactamase L1 contributes to the development of β-lactam antibiotic resistance. The metal-β-lactamase inhibitor can restore the efficacy of β-lactam antibiotics, and its developm...As a zinc-dependent enzyme, metal-β-lactamase L1 contributes to the development of β-lactam antibiotic resistance. The metal-β-lactamase inhibitor can restore the efficacy of β-lactam antibiotics, and its development has attracted much attention. In the present study, we used four widely-used virtual screening programs to screen 7035 small molecules to identify potential L1 inhibitors, and a high-throughput experimental model of L1 inhibitors was established. In this high-throughput testing model, the inhibition rate of 163 compounds on L1 exceeded 40%. The results of virtual screening of 7035 small molecules using the following four programs showed that among the top 1.35% of the compounds, their hit rates were ranked as Schr?dinger’s(5.26%), DS(1.05%), and Sybyl-x 2.0(1.05%), and Smina(2.11%).展开更多
文摘目的:探讨重症监护病房(intensive care unit,ICU)患者铜绿假单胞菌IMP-1耐药基因检测及耐药性,为临床合理使用抗生素提供依据。方法:采用聚合酶链反应(PCR)检测耐药基因IMP-1,ATB Expression自动鉴定病原菌及药敏分析。结果:2007～2009年共分离出106株铜绿假单胞菌,对头孢他啶、亚胺培南、哌拉西林、氨曲南、头孢哌酮、头孢吡肟、左氧氟沙星的耐药率分别为50.9%、39.6%、79.2%、56.6%、57.5%、41.5%、25.5%。每年耐亚胺培南株的检出率依序分别为16.0%、28.3%、39.6%。耐亚胺培南株绝大部分检出IMP耐药基因。结论:ICU患者金属酶表型阳性菌株检出的基因型主要为IMP-1,产酶菌株耐药性强,随时进行细菌耐药性监测,正确使用有效的抗生素是控制感染和延缓细菌耐药的关键。
基金Natural Sciences Foundation of China (Grant No. 81872913)National High-tech R&D Program (863 Program, Grant No. 2015AA020911)。
文摘As a zinc-dependent enzyme, metal-β-lactamase L1 contributes to the development of β-lactam antibiotic resistance. The metal-β-lactamase inhibitor can restore the efficacy of β-lactam antibiotics, and its development has attracted much attention. In the present study, we used four widely-used virtual screening programs to screen 7035 small molecules to identify potential L1 inhibitors, and a high-throughput experimental model of L1 inhibitors was established. In this high-throughput testing model, the inhibition rate of 163 compounds on L1 exceeded 40%. The results of virtual screening of 7035 small molecules using the following four programs showed that among the top 1.35% of the compounds, their hit rates were ranked as Schr?dinger’s(5.26%), DS(1.05%), and Sybyl-x 2.0(1.05%), and Smina(2.11%).
