目的探讨抑制素βA(inhibinβA,INHβA)在胃癌中过表达的临床意义。方法采用免疫组化检测146例胃癌组织芯片中INHβA的表达,采用免疫组化和荧光原位杂交(fluorescence in situ hybridization,FISH)检测HER2蛋白过表达和基因扩增,并结合...目的探讨抑制素βA(inhibinβA,INHβA)在胃癌中过表达的临床意义。方法采用免疫组化检测146例胃癌组织芯片中INHβA的表达,采用免疫组化和荧光原位杂交(fluorescence in situ hybridization,FISH)检测HER2蛋白过表达和基因扩增,并结合患者的随访资料进行预后分析。结果61.6%(90/146)的胃癌呈INHβA过表达,显著高于正常胃黏膜(P<0.01)。胃癌中INHβA表达在不同肿瘤大小(P<0.05)、分化程度(P<0.01)、临床分期(P<0.05)以及有无淋巴结转移(P<0.01)之间差异有统计学意义。19.2%(28/146)和16.4%(24/146)的病例分别呈HER2蛋白表达和基因扩增,HER2蛋白表达/基因扩增在不同肿瘤部位、组织学类型之间表达差异有统计学意义(P<0.01)。INHβA过表达与HER2蛋白表达及基因扩增均显著负相关(分别r=-0.224,P<0.01;r=-0.175,P<0.05)。生存分析显示,INHβA+患者5年生存率显著低于INHβA-患者(P<0.05)。根据INHβA和HER2的表达可以将胃癌分为INHβA/HER2双表达组(INHβA+/HER2+)、单表达组(INHβA-/HER2+和INHβA+/HER2-)和双阴性组(HER2-/INHβA-),生存分析显示INHβA/HER2双表达的患者预后最差(P<0.01)。结论INHβA过表达可以作为判断胃癌预后不良的一项重要指标,INHβA和HER2不同表达模式为胃癌的个体化治疗提供了新的思路。展开更多
Sucrose metabolism,particularly the decomposition of sucrose by invertase,plays a central role in plant responses to cold stress.Invertase inhibitors(INHs)evolved in higher plants as essential regulators of sucrose me...Sucrose metabolism,particularly the decomposition of sucrose by invertase,plays a central role in plant responses to cold stress.Invertase inhibitors(INHs)evolved in higher plants as essential regulators of sucrose metabolism.By limiting invertase activity,INHs keep cellular sugar levels elevated,which provides enhanced protection to plants under stress.Our results showed that the expression of PpVIN2,the only vacuolar invertase(VIN)gene in peach fruit sensitive to chilling temperatures,increases significantly during cold storage,while VIN enzyme activity increases more modestly.We also found that peach fruit transiently overexpressing PpINH1 had decreased VIN activity.Interactions of PpINH1 and PpVIN2 with recombinant proteins were shown by yeast two-hybrid assays and bimolecular fluorescence complementation assays,as well as in vitro.During cold storage,trehalose-treated peach fruit had significantly increased PpINH1 expression,decreased VIN enzyme activity,and significantly higher sucrose content than did untreated fruit.As a result,the treated fruit had enhanced resistance to chilling injury.Collectively,our data show that the post-translational repression of VIN enzyme activity by PpINH1 helps maintain sucrose levels in peach fruit during cold storage,thereby improving resistance to chilling injury.展开更多
By in vitro visible electron paramagnetic resonance (EPR) spectrophotometry method we demonstrated that N-isonicotinoyl-N’-(3-etoxy-2-hydroxybenzaldehyd) hydrazone (SH11) exhibits radical scavenging activity (SSA). M...By in vitro visible electron paramagnetic resonance (EPR) spectrophotometry method we demonstrated that N-isonicotinoyl-N’-(3-etoxy-2-hydroxybenzaldehyd) hydrazone (SH11) exhibits radical scavenging activity (SSA). Malondialdehyde (MDA) in mice treated with INH was increased (2.578 ± 0.349 mM vs. 2.024 ± 0.164 μM, p SOD/mg Pr vs. 2.273 ± 0.317 USOD/mg Pr, p CAT/mg Pr, vs. 47.070 ± 16.490 UCAT/mg Pr, p < 0.05) were decreased, compared to the untreated controls. The combination INH + SH11 (30 mg/kg p.o.) showed reduced levels of MDA, compared to the INH-treated (mean 2.291 ± 0.025 μM than 2.578 ± 0.349, p < 0.05). The combination with the lowest reduction of SOD compared to the controls was 151 mg/kg i.p. INH + 30 mg/kg p.o. SH11, but there was a significant difference in SOD activities between the group treated with this combination and the untreated controls (p < 0.05). The most effective combination, with CAT levels, close to the controls was 151 mg/kg i.p. INH + 30 mg/kg p.o. SH11.展开更多
文摘目的探讨抑制素βA(inhibinβA,INHβA)在胃癌中过表达的临床意义。方法采用免疫组化检测146例胃癌组织芯片中INHβA的表达,采用免疫组化和荧光原位杂交(fluorescence in situ hybridization,FISH)检测HER2蛋白过表达和基因扩增,并结合患者的随访资料进行预后分析。结果61.6%(90/146)的胃癌呈INHβA过表达,显著高于正常胃黏膜(P<0.01)。胃癌中INHβA表达在不同肿瘤大小(P<0.05)、分化程度(P<0.01)、临床分期(P<0.05)以及有无淋巴结转移(P<0.01)之间差异有统计学意义。19.2%(28/146)和16.4%(24/146)的病例分别呈HER2蛋白表达和基因扩增,HER2蛋白表达/基因扩增在不同肿瘤部位、组织学类型之间表达差异有统计学意义(P<0.01)。INHβA过表达与HER2蛋白表达及基因扩增均显著负相关(分别r=-0.224,P<0.01;r=-0.175,P<0.05)。生存分析显示,INHβA+患者5年生存率显著低于INHβA-患者(P<0.05)。根据INHβA和HER2的表达可以将胃癌分为INHβA/HER2双表达组(INHβA+/HER2+)、单表达组(INHβA-/HER2+和INHβA+/HER2-)和双阴性组(HER2-/INHβA-),生存分析显示INHβA/HER2双表达的患者预后最差(P<0.01)。结论INHβA过表达可以作为判断胃癌预后不良的一项重要指标,INHβA和HER2不同表达模式为胃癌的个体化治疗提供了新的思路。
基金supported by the National Key R&D Program of China(No.2018YFD1000200)National Science Foundation of China(Nos.31671903 and 31972124).
文摘Sucrose metabolism,particularly the decomposition of sucrose by invertase,plays a central role in plant responses to cold stress.Invertase inhibitors(INHs)evolved in higher plants as essential regulators of sucrose metabolism.By limiting invertase activity,INHs keep cellular sugar levels elevated,which provides enhanced protection to plants under stress.Our results showed that the expression of PpVIN2,the only vacuolar invertase(VIN)gene in peach fruit sensitive to chilling temperatures,increases significantly during cold storage,while VIN enzyme activity increases more modestly.We also found that peach fruit transiently overexpressing PpINH1 had decreased VIN activity.Interactions of PpINH1 and PpVIN2 with recombinant proteins were shown by yeast two-hybrid assays and bimolecular fluorescence complementation assays,as well as in vitro.During cold storage,trehalose-treated peach fruit had significantly increased PpINH1 expression,decreased VIN enzyme activity,and significantly higher sucrose content than did untreated fruit.As a result,the treated fruit had enhanced resistance to chilling injury.Collectively,our data show that the post-translational repression of VIN enzyme activity by PpINH1 helps maintain sucrose levels in peach fruit during cold storage,thereby improving resistance to chilling injury.
文摘By in vitro visible electron paramagnetic resonance (EPR) spectrophotometry method we demonstrated that N-isonicotinoyl-N’-(3-etoxy-2-hydroxybenzaldehyd) hydrazone (SH11) exhibits radical scavenging activity (SSA). Malondialdehyde (MDA) in mice treated with INH was increased (2.578 ± 0.349 mM vs. 2.024 ± 0.164 μM, p SOD/mg Pr vs. 2.273 ± 0.317 USOD/mg Pr, p CAT/mg Pr, vs. 47.070 ± 16.490 UCAT/mg Pr, p < 0.05) were decreased, compared to the untreated controls. The combination INH + SH11 (30 mg/kg p.o.) showed reduced levels of MDA, compared to the INH-treated (mean 2.291 ± 0.025 μM than 2.578 ± 0.349, p < 0.05). The combination with the lowest reduction of SOD compared to the controls was 151 mg/kg i.p. INH + 30 mg/kg p.o. SH11, but there was a significant difference in SOD activities between the group treated with this combination and the untreated controls (p < 0.05). The most effective combination, with CAT levels, close to the controls was 151 mg/kg i.p. INH + 30 mg/kg p.o. SH11.