The effect of Procox? (Bayer, emodepside/toltrazuril suspension for dogs) against natural infections with Isospora spp. was investigated. Two groups were treated either with 0.5 ml suspension/kg of body weight (0.45 m...The effect of Procox? (Bayer, emodepside/toltrazuril suspension for dogs) against natural infections with Isospora spp. was investigated. Two groups were treated either with 0.5 ml suspension/kg of body weight (0.45 mg emodepside and 9 mg toltrazuril/kg of body weight) in the 3rd, 5th and 7th week of life (w.o.l.) (Procox? group;n = 28) or with a control anthelminthic (Dewormed Control group;n = 26). Animals were surveyed weekly from the 3rd w.o.l. by coproscopy and clinical examination. Faecal samples were examined microbiologically from the 4th to the 8th w.o.l. and faecal inflammatory markers canine calprotectin and canine S100A12 were measured in the 8th w.o.l. Specific antibody titres were evaluated in serum samples from five litters before and after vaccination against canine distemper virus and canine parvovirus 2. The prevalence of Isospora-positive animals increased to 67% in the Dewormed Control group (n = 15 puppies from four parasite-positive litters), while in the Procox? group (n = 15 puppies) it was less than 34% with significantly lower excretion (p ? was easily applied and effective;adverse effects did not occur. The level of seroconversion or titre increase upon vaccination was higher in parasite-free animals (91%) compared to Procox?-treated puppies (30%) and the Control animals (10%). Animals from parasite-free litters showed significantly different excretion patterns for haemolytic Escherichia coli and Clostridium perfringens, while there was no difference between Procox?-treated and Control animals. In some animals kept under poor hygienic conditions diarrhoea was noted in association with C. perfringens, E. coli or Salmonella. Concentrations of inflammatory markers in the faeces did not significantly differ between the Procox? and the Control group. Adequate control of parasitic and bacterial infections in suckling puppies requires both antiparasitic treatment and hygiene. Even when parasites do not cause overt effects treatment is recommended in cases with a history of parasite infections.展开更多
为建立一种快速、准确检测猫等孢球虫的方法,本研究采用GenBank登录的猫等孢球虫18s r RNA基因序列(L76471.1)保守区设计并合成1对特异引物,从经显微镜检测为猫等孢球虫阳性的粪便样品中选取一份提取猫等孢球虫DNA,以其为模板经PCR扩增...为建立一种快速、准确检测猫等孢球虫的方法,本研究采用GenBank登录的猫等孢球虫18s r RNA基因序列(L76471.1)保守区设计并合成1对特异引物,从经显微镜检测为猫等孢球虫阳性的粪便样品中选取一份提取猫等孢球虫DNA,以其为模板经PCR扩增猫等孢球虫18s r RNA基因片段,构建重组质粒标准品p UCm-T-IF1并经PCR及测序鉴定正确后作为质粒标准品。通过优化反应条件,建立了猫等孢球虫的荧光定量PCR检测方法。特异性试验结果显示,该方法可以特异性的检测猫等孢球虫DNA,对猫贾第虫、猫滴虫、猫细小病毒、猫冠状病毒DNA/cDNA的检测结果均为阴性;对重组质粒标准品的检测限可达6.26×10^(2)拷贝/μL。批内和批间重复性试验的变异系数分别是0.61%~2.06%和0.32%~0.50%。表明本研究建立的方法特异性强、敏感性高、重复性好。利用该方法对23份临床疑似猫等孢球虫感染的样品进行检测,荧光定量PCR的检出率为78.26%(18/23),明显高于漂浮法检测的阳性率43.48%(10/23),并且经漂浮法检测为阳性的样品采用该方法检测均为阳性。结果表明,本研究建立的荧光定量PCR方法能够更准确的检测猫等孢球虫的感染,为开展猫等孢球虫病和流行病学调查提供更准确的检测手段。展开更多
参照GenBank收录的Isospora suis 18SrRNA基因全序列(U97523),利用引物分析软件Oligo 6.57和Primer Premier 5.0设计了一对引物(上游引物:5'-tcctgcgagtactcatatgc-3';下游引物:5'-gttcagc-tacgcataccttg-3'),首次扩增...参照GenBank收录的Isospora suis 18SrRNA基因全序列(U97523),利用引物分析软件Oligo 6.57和Primer Premier 5.0设计了一对引物(上游引物:5'-tcctgcgagtactcatatgc-3';下游引物:5'-gttcagc-tacgcataccttg-3'),首次扩增出猪等孢球虫分离株的18SrRNA基因序列,结合GenBank上登录的相关原虫序列,用DNAstar4.0软件比较其同源性,经Clustalx1.81序列比对,Paup4.0、Treeview3.0、Phylip种系发育关系软件分析后,证实该分离株为猪等孢球虫,并且河南不同地区之间的猪等孢球虫没有明显遗传差异。展开更多
对郑州动物园15只孔雀(pavo)的粪便进行了检查,结果发现它们主要感染鸟等孢球虫Isospora Iacazei Labbe,1893;偶见有梅氏艾美缸球虫Eimeriamayurai Bhatia et Pande,1966和曼德勒艾美尔球虫E.mandali Banik et Ray1964。对这些球虫已孢...对郑州动物园15只孔雀(pavo)的粪便进行了检查,结果发现它们主要感染鸟等孢球虫Isospora Iacazei Labbe,1893;偶见有梅氏艾美缸球虫Eimeriamayurai Bhatia et Pande,1966和曼德勒艾美尔球虫E.mandali Banik et Ray1964。对这些球虫已孢子化卵囊作了描述、摄影绘图,并列出了检索表。展开更多
基金financially supported by Bayer Animal Health,Germany.
文摘The effect of Procox? (Bayer, emodepside/toltrazuril suspension for dogs) against natural infections with Isospora spp. was investigated. Two groups were treated either with 0.5 ml suspension/kg of body weight (0.45 mg emodepside and 9 mg toltrazuril/kg of body weight) in the 3rd, 5th and 7th week of life (w.o.l.) (Procox? group;n = 28) or with a control anthelminthic (Dewormed Control group;n = 26). Animals were surveyed weekly from the 3rd w.o.l. by coproscopy and clinical examination. Faecal samples were examined microbiologically from the 4th to the 8th w.o.l. and faecal inflammatory markers canine calprotectin and canine S100A12 were measured in the 8th w.o.l. Specific antibody titres were evaluated in serum samples from five litters before and after vaccination against canine distemper virus and canine parvovirus 2. The prevalence of Isospora-positive animals increased to 67% in the Dewormed Control group (n = 15 puppies from four parasite-positive litters), while in the Procox? group (n = 15 puppies) it was less than 34% with significantly lower excretion (p ? was easily applied and effective;adverse effects did not occur. The level of seroconversion or titre increase upon vaccination was higher in parasite-free animals (91%) compared to Procox?-treated puppies (30%) and the Control animals (10%). Animals from parasite-free litters showed significantly different excretion patterns for haemolytic Escherichia coli and Clostridium perfringens, while there was no difference between Procox?-treated and Control animals. In some animals kept under poor hygienic conditions diarrhoea was noted in association with C. perfringens, E. coli or Salmonella. Concentrations of inflammatory markers in the faeces did not significantly differ between the Procox? and the Control group. Adequate control of parasitic and bacterial infections in suckling puppies requires both antiparasitic treatment and hygiene. Even when parasites do not cause overt effects treatment is recommended in cases with a history of parasite infections.
文摘为建立一种快速、准确检测猫等孢球虫的方法,本研究采用GenBank登录的猫等孢球虫18s r RNA基因序列(L76471.1)保守区设计并合成1对特异引物,从经显微镜检测为猫等孢球虫阳性的粪便样品中选取一份提取猫等孢球虫DNA,以其为模板经PCR扩增猫等孢球虫18s r RNA基因片段,构建重组质粒标准品p UCm-T-IF1并经PCR及测序鉴定正确后作为质粒标准品。通过优化反应条件,建立了猫等孢球虫的荧光定量PCR检测方法。特异性试验结果显示,该方法可以特异性的检测猫等孢球虫DNA,对猫贾第虫、猫滴虫、猫细小病毒、猫冠状病毒DNA/cDNA的检测结果均为阴性;对重组质粒标准品的检测限可达6.26×10^(2)拷贝/μL。批内和批间重复性试验的变异系数分别是0.61%~2.06%和0.32%~0.50%。表明本研究建立的方法特异性强、敏感性高、重复性好。利用该方法对23份临床疑似猫等孢球虫感染的样品进行检测,荧光定量PCR的检出率为78.26%(18/23),明显高于漂浮法检测的阳性率43.48%(10/23),并且经漂浮法检测为阳性的样品采用该方法检测均为阳性。结果表明,本研究建立的荧光定量PCR方法能够更准确的检测猫等孢球虫的感染,为开展猫等孢球虫病和流行病学调查提供更准确的检测手段。
文摘参照GenBank收录的Isospora suis 18SrRNA基因全序列(U97523),利用引物分析软件Oligo 6.57和Primer Premier 5.0设计了一对引物(上游引物:5'-tcctgcgagtactcatatgc-3';下游引物:5'-gttcagc-tacgcataccttg-3'),首次扩增出猪等孢球虫分离株的18SrRNA基因序列,结合GenBank上登录的相关原虫序列,用DNAstar4.0软件比较其同源性,经Clustalx1.81序列比对,Paup4.0、Treeview3.0、Phylip种系发育关系软件分析后,证实该分离株为猪等孢球虫,并且河南不同地区之间的猪等孢球虫没有明显遗传差异。
文摘对郑州动物园15只孔雀(pavo)的粪便进行了检查,结果发现它们主要感染鸟等孢球虫Isospora Iacazei Labbe,1893;偶见有梅氏艾美缸球虫Eimeriamayurai Bhatia et Pande,1966和曼德勒艾美尔球虫E.mandali Banik et Ray1964。对这些球虫已孢子化卵囊作了描述、摄影绘图,并列出了检索表。
