Sinocalycanthus chmensis, an endangered species endemic to China, is cultivated as an omamental landscape tree in China. However, S. chinensis, Chimonanthus species and Calycanthusfloridus are difficult to be distingu...Sinocalycanthus chmensis, an endangered species endemic to China, is cultivated as an omamental landscape tree in China. However, S. chinensis, Chimonanthus species and Calycanthusfloridus are difficult to be distinguished in seedling market because of their similar morphological characters. In this study, ISSR （inter-simple sequence repeats） were applied to detect S. chinensis from its closely related species. A unique 748-bp band was found in all accessions of S. chinensis. SCAR （sequence characterized amplified regions） markers were created by cloning and sequencing the specific band, and designing a pair of primers to amplify the band of 748 bp. Diagnostic PCRs were performed using the primer pair with the total DNAs ofS. chinensis, Chimonanthus species and C. floridus as templates, with only S. chinensis being able to be amplified. This amplification is not only rapid （results can be obtained in less than 3 h）, but is also easy to perform. Hence it is a feasible method for identifying S. chmensis in seedling market.展开更多
基金Project (No. G2000046806) supported by the National Basic Research Program (973) of China
文摘Sinocalycanthus chmensis, an endangered species endemic to China, is cultivated as an omamental landscape tree in China. However, S. chinensis, Chimonanthus species and Calycanthusfloridus are difficult to be distinguished in seedling market because of their similar morphological characters. In this study, ISSR （inter-simple sequence repeats） were applied to detect S. chinensis from its closely related species. A unique 748-bp band was found in all accessions of S. chinensis. SCAR （sequence characterized amplified regions） markers were created by cloning and sequencing the specific band, and designing a pair of primers to amplify the band of 748 bp. Diagnostic PCRs were performed using the primer pair with the total DNAs ofS. chinensis, Chimonanthus species and C. floridus as templates, with only S. chinensis being able to be amplified. This amplification is not only rapid （results can be obtained in less than 3 h）, but is also easy to perform. Hence it is a feasible method for identifying S. chmensis in seedling market.
