Objective:To investigate the infection of Fasciola gigantka(F.gigantka)in domestic cattle from Chiang Mai province and molecular confirmation using ITS-2 region.Methods:The liver and gall bladder of Bubalus bubalis(B....Objective:To investigate the infection of Fasciola gigantka(F.gigantka)in domestic cattle from Chiang Mai province and molecular confirmation using ITS-2 region.Methods:The liver and gall bladder of Bubalus bubalis(B.bubalis)and Bos taurus(B.taunts)from slaughterhouses were examined adult worms and prevalence investigation.The species confirmation with phylogenetic analysis using ITS-2 sequences was performed by maximum likelihood and UPGMA methods.Results:The total prevalences of infection in B.bubalis and Bubalus taurus(B.taurus)were67.27%and 52.94%respectively.The respective prevalence in both B.bubalis and B.taurus were acquired from Doi-Saket,Muang,and Sanpatong districts,with 81.25%,62.50%and 60.00%for B.bubalis and 62.50%,50.00%and 47.06%for Bos taunts respectively.The species confirmation of F.gigantka and some related species by basing on maximum likelihood and UPGMA methods used,4 groups of trematodes were generated,first F.gigantka group including specimen of Chiang Mai,second 2 samples of F.hepatica,third group of 3 rumen flukes;Orthocoelium streptocoelium,F.elongatus and Paramphistomum epliclitum and fourth group of 3 minute intestinal flukes:Haplorchis taichui,Stellantchasmu falcatus.Haplorchoides sp.and liver fluke;Opisthorchis civerrini respectively.Conclusions:These results can be confirmed the Giant liver fluke which mainly caused fascioliasis in Chiang Mai was identified as F.gigantka and specimens were the same as those of F.gigantka recorded in olher different countries.Nucleotide sequence of ITS-2 region has been proven as effective diagnostic tool for the identification of F.gigantka.展开更多
The second internal transcribed spacer (ITS-2) of Leucocytozoon caulleryi was amplified by PCR using a pair of conserved primers and cloned into the T-T windows of plasmid pGEM-T easy vector. The inserts were successf...The second internal transcribed spacer (ITS-2) of Leucocytozoon caulleryi was amplified by PCR using a pair of conserved primers and cloned into the T-T windows of plasmid pGEM-T easy vector. The inserts were successfully sequenced and the results revealed that the ITS-2 plus flanking sequence (ITS2+) was composed of 270 nucleotides. and the ITS-2 was 113 bp in length. The ITS-2 of L. caulleryi was analysed by NCBI Blast, and the degree of homology of the ITS-2 of L. caulleryi was compared with that of Eimeria tenella , Candida tropicalis and Saccharomyces kluyveri and others by wDNASIS. The results showed that the ITS-2 of L. caulleryi is characteristic and has the greatest similarity with E. tenella (21.2%).展开更多
文摘Objective:To investigate the infection of Fasciola gigantka(F.gigantka)in domestic cattle from Chiang Mai province and molecular confirmation using ITS-2 region.Methods:The liver and gall bladder of Bubalus bubalis(B.bubalis)and Bos taurus(B.taunts)from slaughterhouses were examined adult worms and prevalence investigation.The species confirmation with phylogenetic analysis using ITS-2 sequences was performed by maximum likelihood and UPGMA methods.Results:The total prevalences of infection in B.bubalis and Bubalus taurus(B.taurus)were67.27%and 52.94%respectively.The respective prevalence in both B.bubalis and B.taurus were acquired from Doi-Saket,Muang,and Sanpatong districts,with 81.25%,62.50%and 60.00%for B.bubalis and 62.50%,50.00%and 47.06%for Bos taunts respectively.The species confirmation of F.gigantka and some related species by basing on maximum likelihood and UPGMA methods used,4 groups of trematodes were generated,first F.gigantka group including specimen of Chiang Mai,second 2 samples of F.hepatica,third group of 3 rumen flukes;Orthocoelium streptocoelium,F.elongatus and Paramphistomum epliclitum and fourth group of 3 minute intestinal flukes:Haplorchis taichui,Stellantchasmu falcatus.Haplorchoides sp.and liver fluke;Opisthorchis civerrini respectively.Conclusions:These results can be confirmed the Giant liver fluke which mainly caused fascioliasis in Chiang Mai was identified as F.gigantka and specimens were the same as those of F.gigantka recorded in olher different countries.Nucleotide sequence of ITS-2 region has been proven as effective diagnostic tool for the identification of F.gigantka.
基金supported by grants from National Natural Science Foundation of China(39870549)Natural Science Foundation of Guangdong Province(980134).
文摘The second internal transcribed spacer (ITS-2) of Leucocytozoon caulleryi was amplified by PCR using a pair of conserved primers and cloned into the T-T windows of plasmid pGEM-T easy vector. The inserts were successfully sequenced and the results revealed that the ITS-2 plus flanking sequence (ITS2+) was composed of 270 nucleotides. and the ITS-2 was 113 bp in length. The ITS-2 of L. caulleryi was analysed by NCBI Blast, and the degree of homology of the ITS-2 of L. caulleryi was compared with that of Eimeria tenella , Candida tropicalis and Saccharomyces kluyveri and others by wDNASIS. The results showed that the ITS-2 of L. caulleryi is characteristic and has the greatest similarity with E. tenella (21.2%).
