目的探讨IWR-1对人肝癌细胞株Hep3B细胞增殖的影响及可能的机制。方法用不同浓度IWR-1(2、4、8、16μmol/L)处理Hep3B细胞,CCK-8法检测细胞生长抑制率;流式细胞仪检测细胞周期变化及细胞凋亡率;Western blot法检测细胞中蛋白表达的变化...目的探讨IWR-1对人肝癌细胞株Hep3B细胞增殖的影响及可能的机制。方法用不同浓度IWR-1(2、4、8、16μmol/L)处理Hep3B细胞,CCK-8法检测细胞生长抑制率;流式细胞仪检测细胞周期变化及细胞凋亡率;Western blot法检测细胞中蛋白表达的变化,实时定量RT-PCR检测β-catenin和c-myc m RNA表达的变化。结果 IWR-1对人肝癌Hep3B细胞的生长抑制作用呈现剂量和时间依赖性(P<0.01)。流式细胞术结果显示,Hep3B细胞的细胞周期呈现明显的G_0/G_1期阻滞,且细胞凋亡率明显升高(P<0.01),呈现剂量依赖性。IWR-1可引起β-catenin和c-myc m RNA表达下降,β-catenin、c-myc蛋白表达下降,而Axin 1和p-β-catenin蛋白表达上升。结论 IWR-1可以抑制人肝癌细胞株Hep3B的增殖,其机制可能是通过抑制Wnt/β-catenin通路来实现。展开更多
In this contribution,we report the cooperative structure-directing effect of choline hydroxide and aluminosilicate*BEA zeolite in the synthesis of aluminogermanosilicate IWR zeolites for the first time.*BEA zeolites,a...In this contribution,we report the cooperative structure-directing effect of choline hydroxide and aluminosilicate*BEA zeolite in the synthesis of aluminogermanosilicate IWR zeolites for the first time.*BEA zeolites,at variance with any other aluminosilicate zeolites,can serve as heterogeneous seeds for the growth of IWR zeolites and play a cooperative structure-directing role.The crystallization process was investigated using multiple techniques to characterize a series of solid products obtained with various crystallization times.The experiments clearly showed the dissolution of the*BEA zeolite and of an intermediate CDO-type structure.A plausible mechanism for the novel cooperative synthesis has been proposed.The crystallization of the IWR zeolite involves several steps,among which the crucial one is believed to be the reassembly of the building units produced from the decomposition of*BEA zeolite seeds,induced by choline molecules.Having similar structure and common building units(four-,five-,and six-membered rings)with the IWR zeolite,the*BEA zeolite is capable of promoting the reassembly of the building units and can thus play a cooperative structure-directing role.By highlighting the cooperative structure-directing effect of organic molecules and heterogeneous seeds,this study opens up new perspectives for the synthesis of target zeolites that are difficult to prepare by traditional methods.This new synthetic route is also expected to shed light on the discovery of novel zeolites.展开更多
This paper presents a low power design for a 384 x 288 infrared （IR） readout integrated circuit （ROIC）. For the character of IR detector （ ro ≈ 100kΩ, Iint ≈ 100nA）, a novel pixel structure called quad-share ...This paper presents a low power design for a 384 x 288 infrared （IR） readout integrated circuit （ROIC）. For the character of IR detector （ ro ≈ 100kΩ, Iint ≈ 100nA）, a novel pixel structure called quad-share buffered direct injection （QSBDI） is proposed and realized. In QSBDI,four neighbor pixels share one buffered amplifier,which creates high injection efficiency, a stable bias, good FPN performance, and low power usage. This ROIC also supports two integration modes （integration then readout and integration while readout）, two selectable gains, and four window readout modes. A test 128 × 128 ROIC is designed,fabricated,and tested. The test results show that the ROIC has good linearity. The peak to peak variance of the sub array is about 10mV. The power of pixel stage is only lmW,and the total power dissipation is 37mW at a working frequency of 4MHz.展开更多
文摘目的探讨IWR-1对人肝癌细胞株Hep3B细胞增殖的影响及可能的机制。方法用不同浓度IWR-1(2、4、8、16μmol/L)处理Hep3B细胞,CCK-8法检测细胞生长抑制率;流式细胞仪检测细胞周期变化及细胞凋亡率;Western blot法检测细胞中蛋白表达的变化,实时定量RT-PCR检测β-catenin和c-myc m RNA表达的变化。结果 IWR-1对人肝癌Hep3B细胞的生长抑制作用呈现剂量和时间依赖性(P<0.01)。流式细胞术结果显示,Hep3B细胞的细胞周期呈现明显的G_0/G_1期阻滞,且细胞凋亡率明显升高(P<0.01),呈现剂量依赖性。IWR-1可引起β-catenin和c-myc m RNA表达下降,β-catenin、c-myc蛋白表达下降,而Axin 1和p-β-catenin蛋白表达上升。结论 IWR-1可以抑制人肝癌细胞株Hep3B的增殖,其机制可能是通过抑制Wnt/β-catenin通路来实现。
基金supported by the National Key R&D Program of China(2017YFB0702800)National Natural Science Foundation of China(21802168,21503280,21603277)China Petrochemical Corporation(Sinopec Group)~~
文摘In this contribution,we report the cooperative structure-directing effect of choline hydroxide and aluminosilicate*BEA zeolite in the synthesis of aluminogermanosilicate IWR zeolites for the first time.*BEA zeolites,at variance with any other aluminosilicate zeolites,can serve as heterogeneous seeds for the growth of IWR zeolites and play a cooperative structure-directing role.The crystallization process was investigated using multiple techniques to characterize a series of solid products obtained with various crystallization times.The experiments clearly showed the dissolution of the*BEA zeolite and of an intermediate CDO-type structure.A plausible mechanism for the novel cooperative synthesis has been proposed.The crystallization of the IWR zeolite involves several steps,among which the crucial one is believed to be the reassembly of the building units produced from the decomposition of*BEA zeolite seeds,induced by choline molecules.Having similar structure and common building units(four-,five-,and six-membered rings)with the IWR zeolite,the*BEA zeolite is capable of promoting the reassembly of the building units and can thus play a cooperative structure-directing role.By highlighting the cooperative structure-directing effect of organic molecules and heterogeneous seeds,this study opens up new perspectives for the synthesis of target zeolites that are difficult to prepare by traditional methods.This new synthetic route is also expected to shed light on the discovery of novel zeolites.
文摘This paper presents a low power design for a 384 x 288 infrared （IR） readout integrated circuit （ROIC）. For the character of IR detector （ ro ≈ 100kΩ, Iint ≈ 100nA）, a novel pixel structure called quad-share buffered direct injection （QSBDI） is proposed and realized. In QSBDI,four neighbor pixels share one buffered amplifier,which creates high injection efficiency, a stable bias, good FPN performance, and low power usage. This ROIC also supports two integration modes （integration then readout and integration while readout）, two selectable gains, and four window readout modes. A test 128 × 128 ROIC is designed,fabricated,and tested. The test results show that the ROIC has good linearity. The peak to peak variance of the sub array is about 10mV. The power of pixel stage is only lmW,and the total power dissipation is 37mW at a working frequency of 4MHz.
