To evaluate the role of MHC Ⅱ expression in posttrauma immune disturbances and infections. Methods: In experimental study, peritoneal macrophages were harvested from traumatized mice and treated with GM CSF, Ia mo...To evaluate the role of MHC Ⅱ expression in posttrauma immune disturbances and infections. Methods: In experimental study, peritoneal macrophages were harvested from traumatized mice and treated with GM CSF, Ia molecules were determined by flow cytometry. In 24 trauma patients, monocyte HLA DR expression was measured by APAAP technique. Results: Marked inhibition of MHC Ⅱ molecule expression was found in both traumatized mice and patients. In traumatic patients with infection, the inhibition was more severe. Treatment with GM CSF in mice partially restored the Ia expression. Conclusions: Inhibition of MHC Ⅱ molecules plays an important role in postrauma immune disturbances. There is a close relationship between HLA DR inhibition and clinical infections.展开更多
文摘To evaluate the role of MHC Ⅱ expression in posttrauma immune disturbances and infections. Methods: In experimental study, peritoneal macrophages were harvested from traumatized mice and treated with GM CSF, Ia molecules were determined by flow cytometry. In 24 trauma patients, monocyte HLA DR expression was measured by APAAP technique. Results: Marked inhibition of MHC Ⅱ molecule expression was found in both traumatized mice and patients. In traumatic patients with infection, the inhibition was more severe. Treatment with GM CSF in mice partially restored the Ia expression. Conclusions: Inhibition of MHC Ⅱ molecules plays an important role in postrauma immune disturbances. There is a close relationship between HLA DR inhibition and clinical infections.
