Phylogenetic analysis of bacteria in sea ice brine sampled from the Canada Basin,Arctic Ocean

Bacterial diversity in sea ice brine samples which collected from four stations located at the Canada Basin, Arctic Ocean was analyzed by PCR-DGGE. Twenty-three 16S rDNA sequences of bacteria obtained from DGGE bands were cloned and sequenced. Phylogenetic analysis clustered these sequences within γ-proteobacteria, Cytophaga-Flexlbacter-Bacteroides （CFB） group, Firmicutes and Actinobacteria. The phylotype of Pseudoalteromonas in the γ-proteobacteria was predominant and members of the CFB group and γ-proteobacteria were highly abundant in studied sea ice brine samples.

Upper limits for chlorophyll a changes with brine volume in sea ice during the austral spring in the Weddell Sea,Antarctica

During the winter and spring of 2006, we investigated the sea ice physics and marine biology in the northwest Weddell Sea, Antarctica aboard R/V Polarstern. We determined the texture of each ice core and 71 ice crystal thin sections from 27 ice cores. We analyzed 393 ice cores, their temperatures, 348 block density and salinity samples,and 311 chlorophyll a（Chl a） and phaeophytin samples along the cruise route during the investigation. Based on the vertical distributions of 302 groups of data for the ice porosity and Chl a content in the ice at the same position, we obtained new evidence that ice physical parameters influence the Chl a content in ice. We collected snow and ice thickness data, and established the effects of the snow and ice thickness on the Chl a blooms under the ice, as well as the relationships between the activity of ice algae cells and the brine volume in ice according to the principle of environmental control of the ecological balance. We determined the upper limits for Chl a in the brine volume of granular and columnar ice in the Antarctica, thereby demonstrating the effects of ice crystals on brine drainage, and the contributions of the physical properties of sea ice to Chl a blooms near the ice bottom and on the ice-water interface in the austral spring. Moreover, we found that the physical properties of sea ice affect ice algae and they are key control elements that modulate marine phytoplankton blooms in the ice-covered waters around Antarctica.

Ocean Mixing with Lead-Dependent Subgrid Scale Brine Rejection Parameterization in a Climate Model

Sea ice thickness is highly spatially variable and can cause uneven ocean heat and salt flux on subgrid scales in climate models.Previous studies have demonstrated improvements in ocean mixing simulation using parameterization schemes that distribute brine rejection directly in the upper ocean mixed layer.In this study,idealized ocean model experiments were conducted to examine modeled ocean mixing errors as a function of the lead fraction in a climate model grid.When the lead is resolved by the grid,the added salt at the sea surface will sink to the base of the mixed layer and then spread horizontally.When averaged at a climate-model grid size,this vertical distribution of added salt is lead-fraction dependent.When the lead is unresolved,the model errors were systematic leading to greater surface salinity and deeper mixed-layer depth(MLD).An empirical function was developed to revise the added-salt-related parameter n from being fixed to lead-fraction dependent.Application of this new scheme in a climate model showed significant improvement in modeled wintertime salinity and MLD as compared to series of CTD data sets in 1997/1998 and 2006/2007.The results showed the most evident improvement in modeled MLD in the Arctic Basin,similar to that using a fixed n=5,as recommended by the previous Arctic regional model study,in which the parameter n obtained is close to 5 due to the small lead fraction in the Arctic Basin in winter.