Idesia polycarpa Maxim.var vestita Diels.is a dioecious tree species native to eastern Asia.There are diffi culties associated with distinguishing the sex of the plant at the seedling stage.In order to explore the mec...Idesia polycarpa Maxim.var vestita Diels.is a dioecious tree species native to eastern Asia.There are diffi culties associated with distinguishing the sex of the plant at the seedling stage.In order to explore the mechanism of sex diff erentiation in fl ower development,we conducted the transcriptome profi les of male and female fl owers at early,metaphase and late developmental stages.Approximately 123,335 unigenes with a total length of 83,996 Mb and an average length of 168 bp were assembled.The unigenes were blasted into Nr,Nt,Pfam,KOG/COG,Swiss-prot,KEGG,GO databases.Homology analysis demonstrated that I.polycarpa and black cottonwood had the highest homology with the alignment of 92,871 sequences.This study identifi ed 80 groups of transcription factor families with a total of 1475 unigenes,mainly including MYB,WRKY,AP2 and bHLH transcription factor families.KEGG pathway analysis showed that the expression of numerous plant hormones(cytokinin,gibberellin and ethylene)and fl avonoid biosynthesis pathway were diff erent at various stages of female and male fl ower development.In addition,a number of unigenes associated with fl owering were identifi ed which were key genes associated with photoperiodic,vernalization,thermosensory,gibberellin,and autonomic pathways.The results show that I.polycarpa fl oral organ development was in accordance with the ABCDE model,in which the down-regulation of the B gene family might aff ect stamen fertility in late stages of female fl ower development.qRTPCR experiments validated that the expression patterns of 15 unigenes were consistent with those in RNA-seq results.The results highlight a central role for plant sex identifi cation in seedling production and a sex-determining mechanism for dioecious plants.In addition,the transcriptome data provided a theoretical basis for I.polycarpa genetic diversity analysis and molecular-assisted breeding.展开更多
Idesia polycarpa Maxim.var.vestita Diels is an important oil plant with high economic value.In order to improve seed germination and to cultivate high quality seedlings,we set out to identify an optimal medium for its...Idesia polycarpa Maxim.var.vestita Diels is an important oil plant with high economic value.In order to improve seed germination and to cultivate high quality seedlings,we set out to identify an optimal medium for its seedling growth in a plastic cave tray(plug seedling).In this study,four different media including M_(1)(soil),M_(2)(peat∶perlite=2∶1),M_3(peat),and M_4(peat∶perlite∶vermiculite=2∶1∶1)were tested in industrialized plug seedlings.The seedling growth of Idesia polycarpa var.vestita including plant height,crown diameter,leaf number,ground diameter,the relative chlorophyll contents of leaves,etc.was measured.The results indicated that the medium M_(2) was the best for increasing seedling height,crown diameter,leaf counts,ground diameter,and the relative chlorophyll contents of leaves.Therefore,medium M_(2) is recommended in the industrialized nursery process for the seedling growth of Idesia polycarpa var.vestita.展开更多
Idesia polycarpa is a kind of woody oil plant with high quality and yield. It is suitable to be planted in mountainous area. In this study,polyethylene glycol（ PEG） stress environment was used to evaluate the growth...Idesia polycarpa is a kind of woody oil plant with high quality and yield. It is suitable to be planted in mountainous area. In this study,polyethylene glycol（ PEG） stress environment was used to evaluate the growth response of I. polycarpa under drought conditions. The results showed that the seed germination parameters were inhibited to different degrees,and the growth and respiration rate of the seedlings increased slightly under the low concentration of PEG,and obviously decreased when the concentration of PEG was over 15%.展开更多
文摘Idesia polycarpa Maxim.var vestita Diels.is a dioecious tree species native to eastern Asia.There are diffi culties associated with distinguishing the sex of the plant at the seedling stage.In order to explore the mechanism of sex diff erentiation in fl ower development,we conducted the transcriptome profi les of male and female fl owers at early,metaphase and late developmental stages.Approximately 123,335 unigenes with a total length of 83,996 Mb and an average length of 168 bp were assembled.The unigenes were blasted into Nr,Nt,Pfam,KOG/COG,Swiss-prot,KEGG,GO databases.Homology analysis demonstrated that I.polycarpa and black cottonwood had the highest homology with the alignment of 92,871 sequences.This study identifi ed 80 groups of transcription factor families with a total of 1475 unigenes,mainly including MYB,WRKY,AP2 and bHLH transcription factor families.KEGG pathway analysis showed that the expression of numerous plant hormones(cytokinin,gibberellin and ethylene)and fl avonoid biosynthesis pathway were diff erent at various stages of female and male fl ower development.In addition,a number of unigenes associated with fl owering were identifi ed which were key genes associated with photoperiodic,vernalization,thermosensory,gibberellin,and autonomic pathways.The results show that I.polycarpa fl oral organ development was in accordance with the ABCDE model,in which the down-regulation of the B gene family might aff ect stamen fertility in late stages of female fl ower development.qRTPCR experiments validated that the expression patterns of 15 unigenes were consistent with those in RNA-seq results.The results highlight a central role for plant sex identifi cation in seedling production and a sex-determining mechanism for dioecious plants.In addition,the transcriptome data provided a theoretical basis for I.polycarpa genetic diversity analysis and molecular-assisted breeding.
基金Supported by the grant from forestry science and technology innovation research project of Anhui province,China(AHLYCX-2021-07)。
文摘Idesia polycarpa Maxim.var.vestita Diels is an important oil plant with high economic value.In order to improve seed germination and to cultivate high quality seedlings,we set out to identify an optimal medium for its seedling growth in a plastic cave tray(plug seedling).In this study,four different media including M_(1)(soil),M_(2)(peat∶perlite=2∶1),M_3(peat),and M_4(peat∶perlite∶vermiculite=2∶1∶1)were tested in industrialized plug seedlings.The seedling growth of Idesia polycarpa var.vestita including plant height,crown diameter,leaf number,ground diameter,the relative chlorophyll contents of leaves,etc.was measured.The results indicated that the medium M_(2) was the best for increasing seedling height,crown diameter,leaf counts,ground diameter,and the relative chlorophyll contents of leaves.Therefore,medium M_(2) is recommended in the industrialized nursery process for the seedling growth of Idesia polycarpa var.vestita.
基金Supported by Scientific Research Project of State Ethnic Affair Commission(14HBZ007)
文摘Idesia polycarpa is a kind of woody oil plant with high quality and yield. It is suitable to be planted in mountainous area. In this study,polyethylene glycol（ PEG） stress environment was used to evaluate the growth response of I. polycarpa under drought conditions. The results showed that the seed germination parameters were inhibited to different degrees,and the growth and respiration rate of the seedlings increased slightly under the low concentration of PEG,and obviously decreased when the concentration of PEG was over 15%.
