[Objectives] To explore the effects of Qishi Shengjiang Guiyuan Granules on inflammatory response and T lymphocyte subsets in peripheral blood of rats with idiopathic pulmonary fibrosis (IFP) associated with gastroeso...[Objectives] To explore the effects of Qishi Shengjiang Guiyuan Granules on inflammatory response and T lymphocyte subsets in peripheral blood of rats with idiopathic pulmonary fibrosis (IFP) associated with gastroesophageal reflux disease (GERD).[Methods] Twenty-four SPF SD rats were randomly divided into control group, model group, Chinese medicine group and western medicine group, with 6 rats in each group. Except the control group, the other three groups were used to establish the rat model of GERD combined with IPF by injecting hydrochloric acid into the lower end of esophagus and inhaling diluted bleomycin (5 mg/kg). Rats in the Chinese medicine group (14 g/kg), rats in the western medicine group (4.17 g/kg), rats in the control group and the model group were given the same volume of saline by gavage for 14 d. Morphological and pathological changes of esophageal and lung tissues were observed under light microscope, and T lymphocyte subsets (CD + 3, CD + 4, CD + 8) and the ratio of CD + 4/CD + 8 in peripheral blood were detected by flow cytometry.[Results] Compared with the control group, the pulmonary tissue of the model group showed that the pulmonary interstitium was obviously thickened, the alveoli were mutually fused, the structure was obviously destroyed, the original alveolar structure was disappeared, the inflammatory cell infiltration was around the pulmonary capillaries and the alveolar space, and the basal cell hyperplasia and inflammatory cell infiltration were at the lower end of the esophagus. Compared with the model group, the degree of inflammatory cell infiltration and lung tissue damage in the Chinese medicine group and the western medicine group was significantly reduced, the inflammatory infiltration in the lower esophagus was significantly reduced, and the cell proliferation was reduced. Compared with the control group, the CD + 3, CD + 4, CD + 8, CD + 4/CD + 8 in the peripheral blood of the rats in the model group, the Chinese medicine group and the western medicine group decreased ( P <0.01). Compared with the model group, CD + 3, CD + 4, and CD + 4/CD + 8 increased ( P >0.05, P >0.05, P <0.01), CD + 8 decreased ( P >0.05). Compared with the Chinese medicine group, CD + 3, CD + 4, and CD + 4/CD + 8 increased ( P >0.05, P >0.05, P <0.01) and CD + 8 decreased ( P >0.05) in the western medicine group.[Conclusions] Qishi Shengjiang Guiyuan Granules can effectively improve the inflammation of the lower esophagus and lung tissues of the pulmonary fibrosis rats with GERD and IFP, and regulate the number of T lymphocyte subsets CD + 3, CD + 4, CD + 8 cells and CD + 4/CD + 8 ratio in peripheral blood.展开更多
INTRODUCTIONIdiopathic puhnonary fibrosis （IPF） is a specific type of chronic progressive fibrosing interstitial pneumonia associated with a histopathologic pattern of usual interstitial pneumonia. IPF is typically ...INTRODUCTIONIdiopathic puhnonary fibrosis （IPF） is a specific type of chronic progressive fibrosing interstitial pneumonia associated with a histopathologic pattern of usual interstitial pneumonia. IPF is typically considered to be a lung-limited disorder. Although the primary manifestations of IPF are pulmonary in nature. IPF is increasingly recognized as a systemic disease due to its strong association with comorbiditics and coml）lications.展开更多
目的:系统评价慢性阻塞性肺疾病(COPD)病人胃食管反流(GERD)的发生率,为临床护理重视防范胃食管反流的发生、制定适宜的干预方案提供数据支持。方法:计算机检索the Cochrane Library、PubMed、Web of Science、中国知网(CNKI)、中国生...目的:系统评价慢性阻塞性肺疾病(COPD)病人胃食管反流(GERD)的发生率,为临床护理重视防范胃食管反流的发生、制定适宜的干预方案提供数据支持。方法:计算机检索the Cochrane Library、PubMed、Web of Science、中国知网(CNKI)、中国生物医学文献数据库(CBM)、万方数据库等数据库,收集有关COPD病人发生胃食管反流的观察性研究,检索时间为建库至2021年11月1日。由2名研究员独立筛选文献、提取资料并对纳入文献进行质量评价,符合纳入标准的文献应用Stata 15.0软件进行系统评价,采用固定效应或随机效应模型进行数据合并。结果:最终纳入12篇文献,涉及2404例病人。系统评价结果显示,COPD人群胃食管反流的总体发生率为29%[95%CI(0.26,0.32),P<0.001]。亚组分析结果显示,中、重度COPD者发生率为29%,较轻度者25%更高;男性发生率为30%,高于女性的28%;西方地区发生率为37%,高于亚洲地区的27%;采用食道镜检诊断胃食管反流时,其发生率为30%,高于反流症状频率量表(FSSG)评定的28%以及胃食管反流病问卷(GER)评定的21%等问卷筛查工具。结论:现有证据表明,COPD人群中胃食管反流的发生率较高,且存在较强的个体差异。护理人员在临床工作中不仅要重视对该人群行胃食管反流早期筛查,更要加强早期识别胃食管反流发生的危险因素,制定合理的护理干预计划,以改善COPD病人预后,提高生活质量。展开更多
基金Supported by Young and Middle-aged Teachers Scientific Research Basic Ability Promotion Project in Universities of Guangxi(2021 KY0309)Self-funded Scientific Research Project of Guangxi Zhuang Autonomous Region Administration of Traditional Chinese Medicine(GXZYZ20210266)+1 种基金School-level Scientific Research Project of Guangxi University of Chinese Medicine(2021QN016)Hospital-level Scientific Research Project of the First Affiliated Hospital of Guangxi University of Chinese Medicine(2020QN001).
文摘[Objectives] To explore the effects of Qishi Shengjiang Guiyuan Granules on inflammatory response and T lymphocyte subsets in peripheral blood of rats with idiopathic pulmonary fibrosis (IFP) associated with gastroesophageal reflux disease (GERD).[Methods] Twenty-four SPF SD rats were randomly divided into control group, model group, Chinese medicine group and western medicine group, with 6 rats in each group. Except the control group, the other three groups were used to establish the rat model of GERD combined with IPF by injecting hydrochloric acid into the lower end of esophagus and inhaling diluted bleomycin (5 mg/kg). Rats in the Chinese medicine group (14 g/kg), rats in the western medicine group (4.17 g/kg), rats in the control group and the model group were given the same volume of saline by gavage for 14 d. Morphological and pathological changes of esophageal and lung tissues were observed under light microscope, and T lymphocyte subsets (CD + 3, CD + 4, CD + 8) and the ratio of CD + 4/CD + 8 in peripheral blood were detected by flow cytometry.[Results] Compared with the control group, the pulmonary tissue of the model group showed that the pulmonary interstitium was obviously thickened, the alveoli were mutually fused, the structure was obviously destroyed, the original alveolar structure was disappeared, the inflammatory cell infiltration was around the pulmonary capillaries and the alveolar space, and the basal cell hyperplasia and inflammatory cell infiltration were at the lower end of the esophagus. Compared with the model group, the degree of inflammatory cell infiltration and lung tissue damage in the Chinese medicine group and the western medicine group was significantly reduced, the inflammatory infiltration in the lower esophagus was significantly reduced, and the cell proliferation was reduced. Compared with the control group, the CD + 3, CD + 4, CD + 8, CD + 4/CD + 8 in the peripheral blood of the rats in the model group, the Chinese medicine group and the western medicine group decreased ( P <0.01). Compared with the model group, CD + 3, CD + 4, and CD + 4/CD + 8 increased ( P >0.05, P >0.05, P <0.01), CD + 8 decreased ( P >0.05). Compared with the Chinese medicine group, CD + 3, CD + 4, and CD + 4/CD + 8 increased ( P >0.05, P >0.05, P <0.01) and CD + 8 decreased ( P >0.05) in the western medicine group.[Conclusions] Qishi Shengjiang Guiyuan Granules can effectively improve the inflammation of the lower esophagus and lung tissues of the pulmonary fibrosis rats with GERD and IFP, and regulate the number of T lymphocyte subsets CD + 3, CD + 4, CD + 8 cells and CD + 4/CD + 8 ratio in peripheral blood.
文摘INTRODUCTIONIdiopathic puhnonary fibrosis （IPF） is a specific type of chronic progressive fibrosing interstitial pneumonia associated with a histopathologic pattern of usual interstitial pneumonia. IPF is typically considered to be a lung-limited disorder. Although the primary manifestations of IPF are pulmonary in nature. IPF is increasingly recognized as a systemic disease due to its strong association with comorbiditics and coml）lications.
