The aim of the present study was to confirm observations on the vertical transmission of Schistosoma japonicum in the rabbit. S. japonicum- infected pregnant rabbits were used in this study. Perfusion of mother rabb...The aim of the present study was to confirm observations on the vertical transmission of Schistosoma japonicum in the rabbit. S. japonicum- infected pregnant rabbits were used in this study. Perfusion of mother rabbits was done 9weeks after infection in order to obtain worm bur- dens in relation to their initial cercarial dose.Anti- schistosoma specific Ig M antibodies in serum samples collected from rabbit kittens were detected by EL ISA.Our results showed that gestation period lasted the norm al2 9- 31days.All the exposed mother rabbits became infected with S. japonicum.Positive Ig M antibody OD values were detected in12 out of the6 0 kittens exam ined (2 0 .0 % ) . In group C and A,4 0 .0 % and 17.9% of the kitten were congenitally infected,re- spectively. 18.1% of the kittens born to mothers infected with a single dose of 2 0 0 cercariae per rabbit were positives;this is not significantly different from that obtained for the 6 0 0 dose group (2 2 .2 % ) .Three randomly selected Ig M+ kittens harbored between one and two adult worm s. The livers of these kittens displayed granulomatous lesions. It is concluded that congenital S. japonicum infection does occur in the rabbit and is affected by the m other stage of pregnancy and to a lesser extent by its infection load.展开更多
【目的】比较壮医莲花针背廊拔罐法、辅舒良鼻喷雾剂以及西替利嗪等治疗变应性鼻炎(AR)的有效性,并对背廊拔罐法的作用机制开展研究。【方法】采用随机序列的生成方法,将200例AR患者按1∶1∶1∶1比例,随机分为壮医莲花针背廊拔罐法组(...【目的】比较壮医莲花针背廊拔罐法、辅舒良鼻喷雾剂以及西替利嗪等治疗变应性鼻炎(AR)的有效性,并对背廊拔罐法的作用机制开展研究。【方法】采用随机序列的生成方法,将200例AR患者按1∶1∶1∶1比例,随机分为壮医莲花针背廊拔罐法组(试验组)、辅舒良组(对照A组)、西替利嗪组(对照B组)和空白对照组,每组各50例。试验组给予壮医莲花针叩刺背廊拔罐法治疗,对照A组给予辅舒良鼻喷雾剂喷鼻治疗,对照B组给予口服西替利嗪片治疗,空白对照组不接受任何治疗,各组疗程均为10 d,停1周后继续第2个疗程,2个疗程后观察各组的临床疗效及对特异性Ig E(S-Ig E)、白三烯(LT)、白细胞介素-4(IL-4)、IL-9 m RNA、干扰素-γ(IFN-γ)、Thl/Th2细胞、Th17细胞因子(IL-17)等的影响。【结果】(1)经2个疗程治疗后,试验组疗效优于各对照组,对照A组和对照B组疗效优于空白对照组(均P<0.05),而对照A组与对照B组比较,差异无统计学意义(P>0.05)。(2)治疗后,各治疗组的S-Ig E、LT、IL-9 m RNA、IL-4、IL-17水平降低,IFN-γ、Th1/Th2水平升高,与治疗前比较差异均有统计学意义(均P<0.05);而空白对照组治疗前后各指标比较,差异均无统计学意义(P>0.05)。治疗后组间比较,试验组对S-Ig E、LT、IFN-γ、Th1/Th2的改善作用优于对照A组和对照B组(均P<0.05)。(3)在研究过程中,各组均未发现不良反应事件发生。【结论】壮医莲花针背廊拔罐法治疗AR疗效确切,其机制可能为抑制S-Ig E、LT、IL-9 m RNA、IL-17的表达,并通过降低Th2、提高Th1细胞因子水平的表达,调节Th1/Th2失衡而发挥作用。展开更多
文摘The aim of the present study was to confirm observations on the vertical transmission of Schistosoma japonicum in the rabbit. S. japonicum- infected pregnant rabbits were used in this study. Perfusion of mother rabbits was done 9weeks after infection in order to obtain worm bur- dens in relation to their initial cercarial dose.Anti- schistosoma specific Ig M antibodies in serum samples collected from rabbit kittens were detected by EL ISA.Our results showed that gestation period lasted the norm al2 9- 31days.All the exposed mother rabbits became infected with S. japonicum.Positive Ig M antibody OD values were detected in12 out of the6 0 kittens exam ined (2 0 .0 % ) . In group C and A,4 0 .0 % and 17.9% of the kitten were congenitally infected,re- spectively. 18.1% of the kittens born to mothers infected with a single dose of 2 0 0 cercariae per rabbit were positives;this is not significantly different from that obtained for the 6 0 0 dose group (2 2 .2 % ) .Three randomly selected Ig M+ kittens harbored between one and two adult worm s. The livers of these kittens displayed granulomatous lesions. It is concluded that congenital S. japonicum infection does occur in the rabbit and is affected by the m other stage of pregnancy and to a lesser extent by its infection load.
文摘【目的】比较壮医莲花针背廊拔罐法、辅舒良鼻喷雾剂以及西替利嗪等治疗变应性鼻炎(AR)的有效性,并对背廊拔罐法的作用机制开展研究。【方法】采用随机序列的生成方法,将200例AR患者按1∶1∶1∶1比例,随机分为壮医莲花针背廊拔罐法组(试验组)、辅舒良组(对照A组)、西替利嗪组(对照B组)和空白对照组,每组各50例。试验组给予壮医莲花针叩刺背廊拔罐法治疗,对照A组给予辅舒良鼻喷雾剂喷鼻治疗,对照B组给予口服西替利嗪片治疗,空白对照组不接受任何治疗,各组疗程均为10 d,停1周后继续第2个疗程,2个疗程后观察各组的临床疗效及对特异性Ig E(S-Ig E)、白三烯(LT)、白细胞介素-4(IL-4)、IL-9 m RNA、干扰素-γ(IFN-γ)、Thl/Th2细胞、Th17细胞因子(IL-17)等的影响。【结果】(1)经2个疗程治疗后,试验组疗效优于各对照组,对照A组和对照B组疗效优于空白对照组(均P<0.05),而对照A组与对照B组比较,差异无统计学意义(P>0.05)。(2)治疗后,各治疗组的S-Ig E、LT、IL-9 m RNA、IL-4、IL-17水平降低,IFN-γ、Th1/Th2水平升高,与治疗前比较差异均有统计学意义(均P<0.05);而空白对照组治疗前后各指标比较,差异均无统计学意义(P>0.05)。治疗后组间比较,试验组对S-Ig E、LT、IFN-γ、Th1/Th2的改善作用优于对照A组和对照B组(均P<0.05)。(3)在研究过程中,各组均未发现不良反应事件发生。【结论】壮医莲花针背廊拔罐法治疗AR疗效确切,其机制可能为抑制S-Ig E、LT、IL-9 m RNA、IL-17的表达,并通过降低Th2、提高Th1细胞因子水平的表达,调节Th1/Th2失衡而发挥作用。