This study was prepared due to a lack of studies on the immune modulatory effects of prodigiosin in mice against bacterial infection and the increased demand for natural resources. This study aimed to extract prodigio...This study was prepared due to a lack of studies on the immune modulatory effects of prodigiosin in mice against bacterial infection and the increased demand for natural resources. This study aimed to extract prodigiosin from Serratia rubidaea against Citrobacter freundii in mice by evaluating its immunomodulatory activity and histological alterations. A total of twenty-four Swiss mice were divided up into four groups of six mice each. C. freundii (1 × 106 cfu/ml) was administered orally to groups (2, 3 and 4) as an infectious dosage, and one milliliter was administered to the first group as a negative control. Following 24 hours from C. freundii infection, the Group 3 and Group 4 groups were given crude prodigiosin extract in the following dosage amounts: the third and fourth groups received (500 and 1000 µg/ml Intraperitoneal) respectively. ELISA test was performed to assess IgM, IL-6, and IL-10 on days 3, 7 and 14. IgM and IL-6 findings demonstrated a significant increase in Group2 and Group4 with differences (P < 0.05), however, G3 showed a significant decrease when compared to the negative control. The results of IL-10 concentration revealed that Group 2 and Group 4 had significant decreases with differences (P < 0.05), with Group 3 having the highest titer. In conclusion, the study’s findings showed that while prodigiosin’s high concentration can boost the immune system and help laboratory animals resist bacterial infection, its low concentration acts as an immune suppressant.展开更多
文摘目的探讨原发性IgA肾病(IgA nephropathy,IgAN)患者不同IgM沉积强度与肾小球超微结构病变及临床病理的关系。方法收集155例IgAN患者的临床病理资料。分为IgM阴性(51例)、轻度(89例)和中度及以上沉积(15例)3组。比较3组间肾小球超微特征性结构、临床指标、MEST-C评分差异并分析与IgM沉积强度的影响因素。结果与IgM阴性、IgM轻度沉积组相比,IgM中度及以上沉积组24 h尿蛋白水平、IgG沉积、肾小管萎缩/间质纤维化(T)评分、足突融合(foot process effacement,FPE)程度更高,血白蛋白水平、淋巴细胞计数更低(P<0.05)。logistic结果显示FPE程度、T评分是IgM沉积强度的独立影响因素。结论IgM沉积强度与原发性IgAN患者FPE程度相关。FPE程度可协同临床病理评分更精准评估肾组织损伤及制定治疗方案。
文摘This study was prepared due to a lack of studies on the immune modulatory effects of prodigiosin in mice against bacterial infection and the increased demand for natural resources. This study aimed to extract prodigiosin from Serratia rubidaea against Citrobacter freundii in mice by evaluating its immunomodulatory activity and histological alterations. A total of twenty-four Swiss mice were divided up into four groups of six mice each. C. freundii (1 × 106 cfu/ml) was administered orally to groups (2, 3 and 4) as an infectious dosage, and one milliliter was administered to the first group as a negative control. Following 24 hours from C. freundii infection, the Group 3 and Group 4 groups were given crude prodigiosin extract in the following dosage amounts: the third and fourth groups received (500 and 1000 µg/ml Intraperitoneal) respectively. ELISA test was performed to assess IgM, IL-6, and IL-10 on days 3, 7 and 14. IgM and IL-6 findings demonstrated a significant increase in Group2 and Group4 with differences (P < 0.05), however, G3 showed a significant decrease when compared to the negative control. The results of IL-10 concentration revealed that Group 2 and Group 4 had significant decreases with differences (P < 0.05), with Group 3 having the highest titer. In conclusion, the study’s findings showed that while prodigiosin’s high concentration can boost the immune system and help laboratory animals resist bacterial infection, its low concentration acts as an immune suppressant.
