期刊文献+
共找到2篇文章
< 1 >
每页显示 20 50 100
Application of high-titred IgY antibodies in orthopox virus diagnostics 被引量:2
1
作者 张小莺 AndreasKurth +3 位作者 DianaPauly GeorgPauli RdigerSchade HeinzEllerbrok 《Journal of Chinese Pharmaceutical Sciences》 CAS 2008年第3期183-191,共9页
Layer chickens were immunized with three species of inactivated orthopox virus (vaccinia virus, calpox virus and cowpox virus). Antibodies (IgY) were purified from egg yolks by improved polyethylene glycol precipi... Layer chickens were immunized with three species of inactivated orthopox virus (vaccinia virus, calpox virus and cowpox virus). Antibodies (IgY) were purified from egg yolks by improved polyethylene glycol precipitation. The development of IgY directed against orthopox virus antigens was followed by immunofluorescence assay, plaque reduction neutraliztion test and immunoelectron microscopy. Cross-reactivity of two IgY antibodies with cells infected by the different strains of the pox viruses was also investigated by different methods (immunofluorescence assay, plaque reduction neutraliztion test and Western blot). Even in very high dilutions in immunofluorescence assay (titres up to 1:10^6 and 1:10^5, respectively) and persisted on a plateau over 10 months after four booster injections, it was showed that anti-vaccinia virus IgY and anti-calpox virus IgY were positive. Neutralizing activity and ultra-structural detection of antigen with gold-labelled antibodies were respectively observed in plaque reduction neutralization test and immunoelectron microscopy. Western blot analysis revealed specific binding of IgY to virus proteins. Thus, there was cross-reactivity between different orthopox viruses. Finally, orthopox virns-specific IgY antibodies bounded magnetic beads (Dynabead) were used to concentration of orthopox viruses. This study suggests that anti-pox virus IgY could serve as a useful tool for orthopox viruses diagnosis. 展开更多
关键词 Orthopox virus (OPV) Egg yolk antibody igy Diagnostic system
下载PDF
A rapid and convenient dot-immunobinding assay for chicken egg-yolk antibodies
2
作者 GUANG PING RUAN LI MA +4 位作者 XIN SHENG YAO QIAN WEN HONG YUN ZOU WEI LUO XIAO NING WANG 《Journal of Microbiology and Immunology》 2006年第2期153-159,共7页
The dot-immunobinding assay was applied to investigate the characteristics of chicken egg yolk antibodies. This method of assay was proved to be a rapid and simple method to demonstrate and characterize the egg-yolk a... The dot-immunobinding assay was applied to investigate the characteristics of chicken egg yolk antibodies. This method of assay was proved to be a rapid and simple method to demonstrate and characterize the egg-yolk antibody IgY in comparison with the traditional ELISA assay. By using the BandScan software, the gray scale value of dots and the background could be determined. According to the intensity of dots (gray scale value) compared to the standard sample of 10μg, how much IgY remained can be determined in a shorter time. 展开更多
关键词 Egg yolk antibody igy Dot-immunobinding assay
下载PDF
上一页 1 下一页 到第
使用帮助 返回顶部