New triterpenoid saponins from the leaves of Ilex chinensis and their hepatoprotective activity

Seven new triterpenoid saponins,including five ursane-type saponins,ilexchinenosides R–V(1–5),and two oleananetype saponins,ilexchinenosides W–X(6–7),with four known triterpenoid saponins(8–11)were isolated from the leaves of Ilex chinensis.Their structures were elucidated by comprehensive spectroscopic 1 D and 2 D NMR and HR-ESI-MS data.Their sugar moieties were determined by HPLC analysis compared with standards after hydrolysis and derivatization.Compounds 1,2,4,9 and 10 exhibited potential hepatoprotective activity against N-acetyl-p-aminophenol(APAP)-induced Hep G2 cell injury in vitro.

不同施肥处理对4种常绿冬青营养生长和结实的影响

以4种常绿冬青(Ilex chinensis Sims)冬青‘阿拉斯加’、矮生无刺枸骨、冬青‘中国少女’和冬青‘纳瑞’为试验材料,研究了4种施肥处理:高氮素肥、高氮素肥+叶面肥(磷酸二氢钾)、高钾素肥、高钾素肥+叶面肥,对不同冬青品种新抽枝条长度、坐果率、果粒径和果实质量的影响。结果发现,与高钾素肥相比,高氮素肥会显著提高冬青‘中国少女’新抽枝条长度和冬青‘纳瑞’的坐果率,同时会显著降低冬青‘阿拉斯加’和矮生无刺枸骨的果粒径。后期喷施叶面肥会显著增加冬青‘阿拉斯加’果实径大小和质量,显著提高矮生无刺枸骨和冬青‘中国少女’新抽枝条长度以及后者的坐果率,但同时也会降低矮生无刺枸骨和冬青‘纳瑞’坐果率和果实质量。研究表明,园林生产中应根据不同冬青品种营养需求特点制定合适的肥料配比方案,需要强调的是施用叶面肥应据品种而定。