[Objectives] A simple and reliable HPLC fingerprint method was developed for the identification of dried barks of Ilex rotunda and I. godajam. [Methods] Nine batches of dried barks of I. rotunda,and seven batches of d...[Objectives] A simple and reliable HPLC fingerprint method was developed for the identification of dried barks of Ilex rotunda and I. godajam. [Methods] Nine batches of dried barks of I. rotunda,and seven batches of dried barks of I. godajam collected from different pharmacies and arboretums in different regions of China were used to establish fingerprints. The software Similarity Evaluation System of Chromatographic Fingerprints of Traditional Chinese Medicine( 2004 A Edition) was used to evaluate the fingerprints. [Results]The fingerprints of dried barks of I. rotunda and I. godajam were established. Methodological study met the technical requirements of fingerprints. The similarities of the fingerprints of dried barks of I. rotunda and I. godajam were all more than 0. 8 and 0. 9 respectively. There were 31 and 28 common peaks in I. rotunda and I. godajam,which could be classified into two clusters by principal component analysis( PCA) and hierarchical cluster analysis. [Conclusions] The feasibility and advantages of used HPLC fingerprints were verified,and the results indicated that the HPLC fingerprint as a characteristic distinguishing method combining similarity evaluation,principal component analysis and hierarchical cluster analysis can be successfully used to identify the authenticity of dried barks of I. rotunda and I. godajam.展开更多
The effects of Scutellaria baicalensis, Panax notoginseng and Ilex rotunda on the viscosities of CMC and Xanthan gum were researched in this paper. Results showed that the viscosities of CMC decrease rapidly with Pana...The effects of Scutellaria baicalensis, Panax notoginseng and Ilex rotunda on the viscosities of CMC and Xanthan gum were researched in this paper. Results showed that the viscosities of CMC decrease rapidly with Panax notoginseng;the viscosities of CMC decrease with Scutellaria baicalensis;the viscosities of CMC decrease slowly with Ilex rotunda. The viscosities of Xanthan gum are almost stable with Scutellaria baicalensis, Panax notoginseng and Ilex rotunda respectively;and the viscosities of Xanthan gum increase with the increase of the Scutellaria baicalensis concentration. There is a side chain on the molecular chain of Xanthan gum, which can form a rod-shaped double helix complex to protect the main chain. There is no side chain on the molecular chain of CMC, and the main chain is “bare”. So, compared to xanthan gum, the molecular chain of CMC is is more susceptible to external influences.展开更多
基金Supported by Special Project for Scientific Research of General Administration of Quality Supervision,Inspection and Quarantine of the People's Republic of China(201210209)China Agriculture Research System(CARS-21)
文摘[Objectives] A simple and reliable HPLC fingerprint method was developed for the identification of dried barks of Ilex rotunda and I. godajam. [Methods] Nine batches of dried barks of I. rotunda,and seven batches of dried barks of I. godajam collected from different pharmacies and arboretums in different regions of China were used to establish fingerprints. The software Similarity Evaluation System of Chromatographic Fingerprints of Traditional Chinese Medicine( 2004 A Edition) was used to evaluate the fingerprints. [Results]The fingerprints of dried barks of I. rotunda and I. godajam were established. Methodological study met the technical requirements of fingerprints. The similarities of the fingerprints of dried barks of I. rotunda and I. godajam were all more than 0. 8 and 0. 9 respectively. There were 31 and 28 common peaks in I. rotunda and I. godajam,which could be classified into two clusters by principal component analysis( PCA) and hierarchical cluster analysis. [Conclusions] The feasibility and advantages of used HPLC fingerprints were verified,and the results indicated that the HPLC fingerprint as a characteristic distinguishing method combining similarity evaluation,principal component analysis and hierarchical cluster analysis can be successfully used to identify the authenticity of dried barks of I. rotunda and I. godajam.
文摘The effects of Scutellaria baicalensis, Panax notoginseng and Ilex rotunda on the viscosities of CMC and Xanthan gum were researched in this paper. Results showed that the viscosities of CMC decrease rapidly with Panax notoginseng;the viscosities of CMC decrease with Scutellaria baicalensis;the viscosities of CMC decrease slowly with Ilex rotunda. The viscosities of Xanthan gum are almost stable with Scutellaria baicalensis, Panax notoginseng and Ilex rotunda respectively;and the viscosities of Xanthan gum increase with the increase of the Scutellaria baicalensis concentration. There is a side chain on the molecular chain of Xanthan gum, which can form a rod-shaped double helix complex to protect the main chain. There is no side chain on the molecular chain of CMC, and the main chain is “bare”. So, compared to xanthan gum, the molecular chain of CMC is is more susceptible to external influences.
