[Objectives] A simple and reliable HPLC fingerprint method was developed for the identification of dried barks of Ilex rotunda and I. godajam. [Methods] Nine batches of dried barks of I. rotunda,and seven batches of d...[Objectives] A simple and reliable HPLC fingerprint method was developed for the identification of dried barks of Ilex rotunda and I. godajam. [Methods] Nine batches of dried barks of I. rotunda,and seven batches of dried barks of I. godajam collected from different pharmacies and arboretums in different regions of China were used to establish fingerprints. The software Similarity Evaluation System of Chromatographic Fingerprints of Traditional Chinese Medicine( 2004 A Edition) was used to evaluate the fingerprints. [Results]The fingerprints of dried barks of I. rotunda and I. godajam were established. Methodological study met the technical requirements of fingerprints. The similarities of the fingerprints of dried barks of I. rotunda and I. godajam were all more than 0. 8 and 0. 9 respectively. There were 31 and 28 common peaks in I. rotunda and I. godajam,which could be classified into two clusters by principal component analysis( PCA) and hierarchical cluster analysis. [Conclusions] The feasibility and advantages of used HPLC fingerprints were verified,and the results indicated that the HPLC fingerprint as a characteristic distinguishing method combining similarity evaluation,principal component analysis and hierarchical cluster analysis can be successfully used to identify the authenticity of dried barks of I. rotunda and I. godajam.展开更多
Total flavonoids extracted from large-leaved Kuding tea(Ilex latifolia Thunb.) were investigated to develop anti-UV sun-screening agents. The results showed that total flavonoids of I. latifolia Thunb.(TFILT) showed m...Total flavonoids extracted from large-leaved Kuding tea(Ilex latifolia Thunb.) were investigated to develop anti-UV sun-screening agents. The results showed that total flavonoids of I. latifolia Thunb.(TFILT) showed maximum absorb peak at 325 nm between UVB and UVA by wavelength scanning spectrum. TFILT could inhibit the decrease of cell viability induced by UVB irradiation by CCK-8 method, and 0.25 mg/ml of TFILT showed the most significant protective activity on cells. The dermal thickness and collagen fiber density as skin structural characteristic were analyzed by Histological staining. The results showed that TFILT protected mice skin by significantly attenuating the dermal thickness and inhibiting collagen fiber degradation. The mechanism in vivo needs to be further confirmed in future.展开更多
Plantation under the forest is a good way of agroforestry,but the canopy closure has a great influence on understory herbs’growth.In the study,different canopy closures of Phyllostachys pubescens forests were set up ...Plantation under the forest is a good way of agroforestry,but the canopy closure has a great influence on understory herbs’growth.In the study,different canopy closures of Phyllostachys pubescens forests were set up to explore its influence on the growth of Ilex latifolia Thunb.The photosynthetic characteristics of Ilex latifolia leaves under different canopy closures were determined by Li-6400 portable photosynthetic system.The results showed that the net photosynthetic rate curve of Ilex latifolia leaves of T1(canopy closure of 0.56)was bimodal with an obvious"midday depression"phenomenon,while the net photosynthetic rate curves of T2(canopy closure of 0.72)and T3(canopy closure of 0.86)were unimodal.The results of light response curve showed that the photosynthetically active radiation and transpiration rate reduced with the increasing of canopy closures.The photosynthetically active radiation,transpiration rate,stomatal conductance,and net photosynthetic rate of Ilex latifolia leaves of T2 were higher than those of T3.Although the net photosynthetic rate of T2 was lower than that of T1,it had no obvious photo-inhibition which affected plant growth.Overall,the canopy closure of 0.72 was more suitable for the growth of Ilex latifolia.The herb plantation in the bamboo forest should be considered with the canopy closure for a better growth.展开更多
The effects of Scutellaria baicalensis, Panax notoginseng and Ilex rotunda on the viscosities of CMC and Xanthan gum were researched in this paper. Results showed that the viscosities of CMC decrease rapidly with Pana...The effects of Scutellaria baicalensis, Panax notoginseng and Ilex rotunda on the viscosities of CMC and Xanthan gum were researched in this paper. Results showed that the viscosities of CMC decrease rapidly with Panax notoginseng;the viscosities of CMC decrease with Scutellaria baicalensis;the viscosities of CMC decrease slowly with Ilex rotunda. The viscosities of Xanthan gum are almost stable with Scutellaria baicalensis, Panax notoginseng and Ilex rotunda respectively;and the viscosities of Xanthan gum increase with the increase of the Scutellaria baicalensis concentration. There is a side chain on the molecular chain of Xanthan gum, which can form a rod-shaped double helix complex to protect the main chain. There is no side chain on the molecular chain of CMC, and the main chain is “bare”. So, compared to xanthan gum, the molecular chain of CMC is is more susceptible to external influences.展开更多
基金Supported by Special Project for Scientific Research of General Administration of Quality Supervision,Inspection and Quarantine of the People's Republic of China(201210209)China Agriculture Research System(CARS-21)
文摘[Objectives] A simple and reliable HPLC fingerprint method was developed for the identification of dried barks of Ilex rotunda and I. godajam. [Methods] Nine batches of dried barks of I. rotunda,and seven batches of dried barks of I. godajam collected from different pharmacies and arboretums in different regions of China were used to establish fingerprints. The software Similarity Evaluation System of Chromatographic Fingerprints of Traditional Chinese Medicine( 2004 A Edition) was used to evaluate the fingerprints. [Results]The fingerprints of dried barks of I. rotunda and I. godajam were established. Methodological study met the technical requirements of fingerprints. The similarities of the fingerprints of dried barks of I. rotunda and I. godajam were all more than 0. 8 and 0. 9 respectively. There were 31 and 28 common peaks in I. rotunda and I. godajam,which could be classified into two clusters by principal component analysis( PCA) and hierarchical cluster analysis. [Conclusions] The feasibility and advantages of used HPLC fingerprints were verified,and the results indicated that the HPLC fingerprint as a characteristic distinguishing method combining similarity evaluation,principal component analysis and hierarchical cluster analysis can be successfully used to identify the authenticity of dried barks of I. rotunda and I. godajam.
基金Supported by Henan Provincial Department of Education (21B350001)Zhengzhou Science and Technology Department (ZZSZX202109ZZSZX202108)。
文摘Total flavonoids extracted from large-leaved Kuding tea(Ilex latifolia Thunb.) were investigated to develop anti-UV sun-screening agents. The results showed that total flavonoids of I. latifolia Thunb.(TFILT) showed maximum absorb peak at 325 nm between UVB and UVA by wavelength scanning spectrum. TFILT could inhibit the decrease of cell viability induced by UVB irradiation by CCK-8 method, and 0.25 mg/ml of TFILT showed the most significant protective activity on cells. The dermal thickness and collagen fiber density as skin structural characteristic were analyzed by Histological staining. The results showed that TFILT protected mice skin by significantly attenuating the dermal thickness and inhibiting collagen fiber degradation. The mechanism in vivo needs to be further confirmed in future.
文摘Plantation under the forest is a good way of agroforestry,but the canopy closure has a great influence on understory herbs’growth.In the study,different canopy closures of Phyllostachys pubescens forests were set up to explore its influence on the growth of Ilex latifolia Thunb.The photosynthetic characteristics of Ilex latifolia leaves under different canopy closures were determined by Li-6400 portable photosynthetic system.The results showed that the net photosynthetic rate curve of Ilex latifolia leaves of T1(canopy closure of 0.56)was bimodal with an obvious"midday depression"phenomenon,while the net photosynthetic rate curves of T2(canopy closure of 0.72)and T3(canopy closure of 0.86)were unimodal.The results of light response curve showed that the photosynthetically active radiation and transpiration rate reduced with the increasing of canopy closures.The photosynthetically active radiation,transpiration rate,stomatal conductance,and net photosynthetic rate of Ilex latifolia leaves of T2 were higher than those of T3.Although the net photosynthetic rate of T2 was lower than that of T1,it had no obvious photo-inhibition which affected plant growth.Overall,the canopy closure of 0.72 was more suitable for the growth of Ilex latifolia.The herb plantation in the bamboo forest should be considered with the canopy closure for a better growth.
文摘The effects of Scutellaria baicalensis, Panax notoginseng and Ilex rotunda on the viscosities of CMC and Xanthan gum were researched in this paper. Results showed that the viscosities of CMC decrease rapidly with Panax notoginseng;the viscosities of CMC decrease with Scutellaria baicalensis;the viscosities of CMC decrease slowly with Ilex rotunda. The viscosities of Xanthan gum are almost stable with Scutellaria baicalensis, Panax notoginseng and Ilex rotunda respectively;and the viscosities of Xanthan gum increase with the increase of the Scutellaria baicalensis concentration. There is a side chain on the molecular chain of Xanthan gum, which can form a rod-shaped double helix complex to protect the main chain. There is no side chain on the molecular chain of CMC, and the main chain is “bare”. So, compared to xanthan gum, the molecular chain of CMC is is more susceptible to external influences.
