Objective:To investigate antibacterial potential of Trifolium alexandrinum(T.alexandrinum) Linn,against seven gram positive and eleven gram negative hospital isolated human pathogenic bacterial strains responsible for...Objective:To investigate antibacterial potential of Trifolium alexandrinum(T.alexandrinum) Linn,against seven gram positive and eleven gram negative hospital isolated human pathogenic bacterial strains responsible for many tropical diseases.Methods:Non-polar and polar extracts of the leaves of T.alexandrinum i.e.,hexane,dichloromethane(DCM),ethyl acetate(EtOAc), methanol(MeOH) and aqueous(AQ) extracts at five different concentrations(1,2,5,10 and 15 mg/ mL) were prepared to evaluate their antibacterial value.NCCL standards were strictly followed to perform antimicrobial disc susceptibility test using disc diffusion method.Results:Polar extracts demonstrated significant antibacterial activity against tested pathogens.EtOAc and MeOH extracts showed maximum antibacterial activity with higher inhibition zone and were found effective against seventeen of the tested pathogens.While AQ plant extract inhibited the growth of sixteen of the test strains.EtOAc and MeOH plant extracts inhibited the growth of all seven gram positive and ten of the gram negative bacterial strains.Conclusions:The present study strongly confirms the effectiveness of crude leaves extracts against tested human pathogenic bacterial strains causing several tropical diseases.Since Egyptian clover is used as a fodder plant,it could be helpful in controlling various infectious diseases associated with cattle as well.展开更多
Mg and its alloys are suitable choices for implant materials due to their biodegradability and biocompatibility features. However, the high electrochemical activity of this family of biomaterials results in their fast...Mg and its alloys are suitable choices for implant materials due to their biodegradability and biocompatibility features. However, the high electrochemical activity of this family of biomaterials results in their fast degradation and severe corrosion in the physiological environment,producing hydrogen(H;) gas, and therefore increasing the p H of the environment. To meet the clinical requirements, the degradation rate of Mg biomaterials needs to be reduced. Nevertheless, higher corrosion resistance of Mg results in a low alkaline p H, weakening the antibacterial activity. Therefore, while the rapid degradation problem of Mg-based biomaterials needs to be addressed, good antibacterial properties are also necessary. By using the plasma electrolytic oxidation(PEO) surface modification technique, the antibacterial activity of Mg and its alloys can be enhanced while maintaining their corrosion protection properties at a high level. Throughout the PEO process, introducing antibacterial agents into solutions results in a major increase in antibacterial activity of the coatings. Moreover, post-or pre-processing on PEO coatings can provide better protection against bacteria. In this review, the antibacterial activity of PEO coatings applied on Mg and also its alloys will be discussed in more detail.展开更多
Objective:To estimate the fatty acids content in the brown algae Padina pavonica(P.pavonica)and Hormophysa triquetra(H.triquetra)and evaluate their potential antimicrobial activity as bioactive compounds.Methods:The f...Objective:To estimate the fatty acids content in the brown algae Padina pavonica(P.pavonica)and Hormophysa triquetra(H.triquetra)and evaluate their potential antimicrobial activity as bioactive compounds.Methods:The fatty acid compositions of the examined species were analyzed using gas chromatography-mass spectrometry.The antimicrobial activity of crude and fatty acids was assessed using the agar plug technique.Results:The fatty acids profile ranged from C8:0 to C20:4.Concentration of saturated fatty acids in P.pavonica was in the order palmitic>myristic>stearic whereas concentration of the unsaturated fatty acids was oleic acid>palmitoleic>9-cis-hexadecenoic>linoleic acid>α-linolenic>arachidonic>elaidic acid.H.triquetra contained high concentration of saturated fatty acids than those of P.pavonica which was in the order as follows:palmitic>margaric>myristic>nonadecyclic>stearic>caprylic>tridecylic>pentadecylic>lauric while the unsaturated fatty acids consisted of oleic>nonadecenoic>non adecadienoate>margaroleic.The crude and fatty acid extracts of H.triquetra and P.pavonica were biologically active on the tested pathogens.H.triquetra exhibited a larger inhibitory zone than P.pavonica.Conclusions:The brown algae P.pavonica and H.triquetra have high efficient amount of fatty acids and showed strong antibacterial activity,especially H.triquetra.展开更多
Antibacterial extract-coated catheters are promising alternatives to their conventional counterparts,but their hemocompatibility and thermal stability must be studied.Nosocomial bacteria have developed resistance to c...Antibacterial extract-coated catheters are promising alternatives to their conventional counterparts,but their hemocompatibility and thermal stability must be studied.Nosocomial bacteria have developed resistance to conventional antibiotics.Herein,the minimum inhibitory but non-hemolytic concentration(MIC-NH)and the thermal stability of Larrea tridentata(L.tridentata)and Origanum vulgare(O.vulgare)extract-coated catheters were studied.Besides,plasma pretreatment was performed to enhance the extract adhesion.Briefly,the extract-coated catheters prevent Staphylococcus aureus colonization without causing hemolysis by using L.tridentata and O.vulgare extracts at MIC-NH(5000 and 2500μg ml~(-1),respectively).Moreover,it has been discovered that the extract coating and plasma treatment improved the thermal stability and the extract adhesion,respectively.Thus,this study provides evidence of alternative antibacterial but non-hemolytic extract-coated catheters.展开更多
基金financially supported by SERC Fast Track OYSSchem,Department of Science and Technology(DST),New Delhi,India[grant No.304/2004(SR/FT/L-129/2004)]
文摘Objective:To investigate antibacterial potential of Trifolium alexandrinum(T.alexandrinum) Linn,against seven gram positive and eleven gram negative hospital isolated human pathogenic bacterial strains responsible for many tropical diseases.Methods:Non-polar and polar extracts of the leaves of T.alexandrinum i.e.,hexane,dichloromethane(DCM),ethyl acetate(EtOAc), methanol(MeOH) and aqueous(AQ) extracts at five different concentrations(1,2,5,10 and 15 mg/ mL) were prepared to evaluate their antibacterial value.NCCL standards were strictly followed to perform antimicrobial disc susceptibility test using disc diffusion method.Results:Polar extracts demonstrated significant antibacterial activity against tested pathogens.EtOAc and MeOH extracts showed maximum antibacterial activity with higher inhibition zone and were found effective against seventeen of the tested pathogens.While AQ plant extract inhibited the growth of sixteen of the test strains.EtOAc and MeOH plant extracts inhibited the growth of all seven gram positive and ten of the gram negative bacterial strains.Conclusions:The present study strongly confirms the effectiveness of crude leaves extracts against tested human pathogenic bacterial strains causing several tropical diseases.Since Egyptian clover is used as a fodder plant,it could be helpful in controlling various infectious diseases associated with cattle as well.
文摘Mg and its alloys are suitable choices for implant materials due to their biodegradability and biocompatibility features. However, the high electrochemical activity of this family of biomaterials results in their fast degradation and severe corrosion in the physiological environment,producing hydrogen(H;) gas, and therefore increasing the p H of the environment. To meet the clinical requirements, the degradation rate of Mg biomaterials needs to be reduced. Nevertheless, higher corrosion resistance of Mg results in a low alkaline p H, weakening the antibacterial activity. Therefore, while the rapid degradation problem of Mg-based biomaterials needs to be addressed, good antibacterial properties are also necessary. By using the plasma electrolytic oxidation(PEO) surface modification technique, the antibacterial activity of Mg and its alloys can be enhanced while maintaining their corrosion protection properties at a high level. Throughout the PEO process, introducing antibacterial agents into solutions results in a major increase in antibacterial activity of the coatings. Moreover, post-or pre-processing on PEO coatings can provide better protection against bacteria. In this review, the antibacterial activity of PEO coatings applied on Mg and also its alloys will be discussed in more detail.
文摘Objective:To estimate the fatty acids content in the brown algae Padina pavonica(P.pavonica)and Hormophysa triquetra(H.triquetra)and evaluate their potential antimicrobial activity as bioactive compounds.Methods:The fatty acid compositions of the examined species were analyzed using gas chromatography-mass spectrometry.The antimicrobial activity of crude and fatty acids was assessed using the agar plug technique.Results:The fatty acids profile ranged from C8:0 to C20:4.Concentration of saturated fatty acids in P.pavonica was in the order palmitic>myristic>stearic whereas concentration of the unsaturated fatty acids was oleic acid>palmitoleic>9-cis-hexadecenoic>linoleic acid>α-linolenic>arachidonic>elaidic acid.H.triquetra contained high concentration of saturated fatty acids than those of P.pavonica which was in the order as follows:palmitic>margaric>myristic>nonadecyclic>stearic>caprylic>tridecylic>pentadecylic>lauric while the unsaturated fatty acids consisted of oleic>nonadecenoic>non adecadienoate>margaroleic.The crude and fatty acid extracts of H.triquetra and P.pavonica were biologically active on the tested pathogens.H.triquetra exhibited a larger inhibitory zone than P.pavonica.Conclusions:The brown algae P.pavonica and H.triquetra have high efficient amount of fatty acids and showed strong antibacterial activity,especially H.triquetra.
基金the grant(No.CVU 859503)given to the first author to pursue his doctorate in Materials Science and Technology at the Autonomous University of Coahuila(UAdeC)FONCYT-Fund Destined to Promote the Development of Science and Technology in the State of Coahuila(No.COAH-2020-C14-C058)。
文摘Antibacterial extract-coated catheters are promising alternatives to their conventional counterparts,but their hemocompatibility and thermal stability must be studied.Nosocomial bacteria have developed resistance to conventional antibiotics.Herein,the minimum inhibitory but non-hemolytic concentration(MIC-NH)and the thermal stability of Larrea tridentata(L.tridentata)and Origanum vulgare(O.vulgare)extract-coated catheters were studied.Besides,plasma pretreatment was performed to enhance the extract adhesion.Briefly,the extract-coated catheters prevent Staphylococcus aureus colonization without causing hemolysis by using L.tridentata and O.vulgare extracts at MIC-NH(5000 and 2500μg ml~(-1),respectively).Moreover,it has been discovered that the extract coating and plasma treatment improved the thermal stability and the extract adhesion,respectively.Thus,this study provides evidence of alternative antibacterial but non-hemolytic extract-coated catheters.
