Objective To analyze the upstream region of radiation-induced junB gene. Methods Four plasmids containing 250 bp, 590 bp, 900 bp and 1650 bp, and CAT reporter gene were constructed separately and introduced to L8704 c...Objective To analyze the upstream region of radiation-induced junB gene. Methods Four plasmids containing 250 bp, 590 bp, 900 bp and 1650 bp, and CAT reporter gene were constructed separately and introduced to L8704 cells. The cells were irradiated with 2 Gy X-rays and incubated at different intervals. Total RNA was extracted from the cells and fluctuation of the CAT mRNA level was assessed by the RNA ratio of CAT/β-actin measured by quantitative Northern blot hybridization. Results CAT mRNA expression containing 900 bp and 1560 bpjunB promoter remarkably and rapidly increased, and reached its peak 30min after 2 Gy X-my irradiation. Conclusions 590-900 bp fragments located in the upstream region of junB gene play an important role in the early process of cells against radiation.展开更多
即刻早期反应基因IEX-1(immediate early response gene X-1)作为即刻早期基因家族之一,参与细胞的多种生物学效应,其转录和表达受多种因素的调控如NF-κB、P53、SP1、AP2、1a-25(OH)2D3、TNF-α及电离辐射等。研究发现,IEX-1在多种肿...即刻早期反应基因IEX-1(immediate early response gene X-1)作为即刻早期基因家族之一,参与细胞的多种生物学效应,其转录和表达受多种因素的调控如NF-κB、P53、SP1、AP2、1a-25(OH)2D3、TNF-α及电离辐射等。研究发现,IEX-1在多种肿瘤细胞中转录及表达降低或缺失,而电离辐射等则可以激活诱导其在多种类型肿瘤细胞和正常细胞中快速的表达,其激活表达与辐射后细胞的生长、周期、凋亡等的改变密切相关。辐射可诱导特性使其具有潜在的临床和基因工程生产应用前景。展开更多
基金This project was supported by Japanese Research Development Corporation (JRDC).
文摘Objective To analyze the upstream region of radiation-induced junB gene. Methods Four plasmids containing 250 bp, 590 bp, 900 bp and 1650 bp, and CAT reporter gene were constructed separately and introduced to L8704 cells. The cells were irradiated with 2 Gy X-rays and incubated at different intervals. Total RNA was extracted from the cells and fluctuation of the CAT mRNA level was assessed by the RNA ratio of CAT/β-actin measured by quantitative Northern blot hybridization. Results CAT mRNA expression containing 900 bp and 1560 bpjunB promoter remarkably and rapidly increased, and reached its peak 30min after 2 Gy X-my irradiation. Conclusions 590-900 bp fragments located in the upstream region of junB gene play an important role in the early process of cells against radiation.
文摘即刻早期反应基因IEX-1(immediate early response gene X-1)作为即刻早期基因家族之一,参与细胞的多种生物学效应,其转录和表达受多种因素的调控如NF-κB、P53、SP1、AP2、1a-25(OH)2D3、TNF-α及电离辐射等。研究发现,IEX-1在多种肿瘤细胞中转录及表达降低或缺失,而电离辐射等则可以激活诱导其在多种类型肿瘤细胞和正常细胞中快速的表达,其激活表达与辐射后细胞的生长、周期、凋亡等的改变密切相关。辐射可诱导特性使其具有潜在的临床和基因工程生产应用前景。
