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THE BINDING BEHAVIOUR OF IMMOBILISED LOW MOLECULAR WEIGHT LIGAND WITH PEPTIDES IN BIOSENSOR-BASED SYSTEM
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作者 Jiang Wei and Hearn Milton T.W. (Monash University, Clayton, VIC 3800. Australia) 《化工学报》 EI CAS CSCD 北大核心 2000年第S1期20-24,共5页
In this study a hovel metal ion affinity ligand was immobility onto the sensor chip. Three poly-histidine peptides were used to study the interaction of tile peptides and the immobilised metal ion affinity ligand via ... In this study a hovel metal ion affinity ligand was immobility onto the sensor chip. Three poly-histidine peptides were used to study the interaction of tile peptides and the immobilised metal ion affinity ligand via biosensor system . The results obtained in this study indicate that the affinity of immobilised Ni(Ⅱ) ion affinity ligand for these peptides appear to be related to the arrangement of the histidine residues in the peptides. This study first documents the application of biosensor technique for paptide screening. 展开更多
关键词 immobilised metal ioll affinity chromatography peptide tags BIOSENSOR interaction of peptide and affinity ligand.
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