Background and objective Previous studies have demonstrated the anti-cancer effects of propolis. However, its use is limited because of its poor bioavailability. In the present study, the major objective was to improv...Background and objective Previous studies have demonstrated the anti-cancer effects of propolis. However, its use is limited because of its poor bioavailability. In the present study, the major objective was to improve propolis bioavailability using a nanosuspension formulation. The cytotoxic effect of propolis nanosuspension (PRO-NS) on the Ehrlich ascites carcinoma (EAC) in female Swiss albino mice was investigated in comparison to the free propolis. Materials and methods A propolis-loaded nanosuspension was formulated by applying solvent-antisolvent nano-precipitation technique. The prepared PRO-NS was characterized for average particle size, polydispersity index (PDI) and zeta potential. Also, the morphology of the nanosuspension particles was investigated using scanning electron microscopy (SEM). Moreover, PRO-NS cytotoxicity was tested using EAC bearing mice. The anticancer activity of Pro-NS was assessed by studying tumor volume, life span, viable and non-viable cell count, antioxidant, biochemical estimations and proliferation of EAC cells. Results The results revealed that propolis nanoparticles were relatively spherical in shape with rough surface. The tumor bearing mice treated with PRO-NS showed increased life span and inhibited tumor growth and the proliferation of EAC cells in comparison to the free propolis (p Conclusions Our results indicate that PRO-NS has a strong inhibitory activity against growth of tumors in comparison to free propolis. The anti-tumor mechanism may be mediated by preventing oxidative damage, immune-stimulation and induction of apoptosis.展开更多
HIV/AIDS is an immune deficiency disease, caused by an RNA virus (positively charged pathogen). It is still being regarded as mysteriously incurable but in Nigeria many patients have been cured (they became HIV-antibo...HIV/AIDS is an immune deficiency disease, caused by an RNA virus (positively charged pathogen). It is still being regarded as mysteriously incurable but in Nigeria many patients have been cured (they became HIV-antibody and antigen negative) by exploiting electrostatic attraction between pathogens and opposite electrical charges that are on Nanoparticles of Medicinal synthetic Aluminum-magnesium silicate (MSAMS) and by using antioxidants to relieve oxidative stress. To confirm the cure, as permanent, a patient (adult male) whose CD4 count increased (P < 0.05) from 685 to 820 while his viral load became undetectable (<20) and he became HIV-negative (antibody and antigen) following the treatment, was tested, every month, post treatment, for HIV-antibody. He has remained HIV-negative for 10 months without being on any antiretroviral medicine (ARV). Longest window period (period HIV-infected person may test antibody-negative) is only 6 months. These results confirm that treatment with the MSAMS and antioxidants normalizes immunity and terminates HIV-infections.展开更多
文摘Background and objective Previous studies have demonstrated the anti-cancer effects of propolis. However, its use is limited because of its poor bioavailability. In the present study, the major objective was to improve propolis bioavailability using a nanosuspension formulation. The cytotoxic effect of propolis nanosuspension (PRO-NS) on the Ehrlich ascites carcinoma (EAC) in female Swiss albino mice was investigated in comparison to the free propolis. Materials and methods A propolis-loaded nanosuspension was formulated by applying solvent-antisolvent nano-precipitation technique. The prepared PRO-NS was characterized for average particle size, polydispersity index (PDI) and zeta potential. Also, the morphology of the nanosuspension particles was investigated using scanning electron microscopy (SEM). Moreover, PRO-NS cytotoxicity was tested using EAC bearing mice. The anticancer activity of Pro-NS was assessed by studying tumor volume, life span, viable and non-viable cell count, antioxidant, biochemical estimations and proliferation of EAC cells. Results The results revealed that propolis nanoparticles were relatively spherical in shape with rough surface. The tumor bearing mice treated with PRO-NS showed increased life span and inhibited tumor growth and the proliferation of EAC cells in comparison to the free propolis (p Conclusions Our results indicate that PRO-NS has a strong inhibitory activity against growth of tumors in comparison to free propolis. The anti-tumor mechanism may be mediated by preventing oxidative damage, immune-stimulation and induction of apoptosis.
文摘HIV/AIDS is an immune deficiency disease, caused by an RNA virus (positively charged pathogen). It is still being regarded as mysteriously incurable but in Nigeria many patients have been cured (they became HIV-antibody and antigen negative) by exploiting electrostatic attraction between pathogens and opposite electrical charges that are on Nanoparticles of Medicinal synthetic Aluminum-magnesium silicate (MSAMS) and by using antioxidants to relieve oxidative stress. To confirm the cure, as permanent, a patient (adult male) whose CD4 count increased (P < 0.05) from 685 to 820 while his viral load became undetectable (<20) and he became HIV-negative (antibody and antigen) following the treatment, was tested, every month, post treatment, for HIV-antibody. He has remained HIV-negative for 10 months without being on any antiretroviral medicine (ARV). Longest window period (period HIV-infected person may test antibody-negative) is only 6 months. These results confirm that treatment with the MSAMS and antioxidants normalizes immunity and terminates HIV-infections.
