The discovery and application of antibiotics in animal feeds have boomed the development of intensive animal husbandry in the last century,until the emergence of antibiotics-resistant bacteria.To alleviate the risks a...The discovery and application of antibiotics in animal feeds have boomed the development of intensive animal husbandry in the last century,until the emergence of antibiotics-resistant bacteria.To alleviate the risks aroused by antibiotics-resistant bacteria,effective antibiotic substitutes are urgently needed to replace antibiotics.Essential oils(EOs)derived from plants are illustrated as the promising antibiotic substitutes used in animal feeds,as same as their current views for poultry and livestock industries in the future.It has been widely demonstrated that the phytochemicals in EOs show multiple biofunctionability and are less likely to induce resistance in bacteria.The beneficial effects of EOs feed supplementation on the intestinal inflammation,intestinal flora,immunity,digestion,and growth performances have been already extensively examined.However,the cost-effectiveness,odor,volatility,instability and bioavailability are the challenges in effectively utilizing EOs in animal intestines.Based on previous researches,and these challenges can partially be resolved by microencapsulation and nanotechnology are promising techniques to deal with these challenges.This article presents the feasibility and foundation of EOs application as antibiotic substitutes in animal feeds,and illustrates the mechanisms,functional performances and superiority of EOs compared with antibiotics.展开更多
To study the resistant mechanism and clinical significance of pseudomonas aeruginosa toβ- lactam antibiotics,the outer mem brane permeability rate of30 P.aeruginosa strains to5 β- lactam antibiotics was m easured ...To study the resistant mechanism and clinical significance of pseudomonas aeruginosa toβ- lactam antibiotics,the outer mem brane permeability rate of30 P.aeruginosa strains to5 β- lactam antibiotics was m easured and their production ofβ- lactamase and theβ- lactam ase genes they carried detected. Furthermore,the relationship between the perm eability,β- lactam ase and the clinical effects ofβ- lactam antibiotics was observed. By using 1 4C- penicillin and liquid- scintillant isotope assay,the affinity of penicillin binding proteins(PBPs) was m easured and their roles in the resistant m echanism studied.Itwas revealed thatthe perm eability rate was higher in sensitive strains than in resistantones(P<0 .0 5 ) .All strains harbored1- 4 β- lactamase genes and produced β- lactam ase.Higher permeability rate and higher degree of stability toβ- lactamase indicated better clinical therapeutic effects. The affinity of PBPs changed little without regard to the perm eability andβ- lactam ase. These results suggested that the permeability of outer mem brane andβ- lacta- mase,but not PBPs,played im portant roles in the resistant mechanism of P. aeruginosa toβ- lac- tam antibiotics and affected the clinical therapeutic effectiveness of som e patients.展开更多
Diaper dermatit is among the widely seen inflamatory skin diseases in neonatals and small 0 - 18 month old children. Control of causative agents as well as providing good genital care may prevent or reduce the risk of...Diaper dermatit is among the widely seen inflamatory skin diseases in neonatals and small 0 - 18 month old children. Control of causative agents as well as providing good genital care may prevent or reduce the risk of the disease. The aim of this study is find out the effect of protective genital care on reducing the diaper dermatit development in 0 - 18 months old inpatient 0 - 18 month old children who are using antibiotic. The study was conducted as prospective half-experimetal using control-experimental groups. Our samples comprise of 0 - 18 month old children admitted at Dokuz Eylul University Practical and Research Hospital, pediatric clinic between January and May 2009, 41 0 - 18 month old children as a control group were not under protective genital care, and 42 0 - 18 month old children as an experimental group were under the protecion and using vaseline. The mothers of the experimental group 0 - 18 month old children were given education. All 0 - 18 month old children were followed-up 3 - 7 days and the collected data were evaluated in computer using Pearson’s chisquare, Fisher’s exact chi-square and Yatest-corrected chi-square tests. The diaper dermatit develop- ment ratio was 34.1% in the control group and 7.1% in the experimental group展开更多
Background:Antibiotic growth promoters are widely used to improve weight gain.However,the abuse of antibiotics can have many negative effects on people.Developing alternatives to antibiotics is an urgent need in lives...Background:Antibiotic growth promoters are widely used to improve weight gain.However,the abuse of antibiotics can have many negative effects on people.Developing alternatives to antibiotics is an urgent need in livestock production.We aimed to perform a meta-analysis and network meta-analysis(NMA)to investigate the effects of feed additives as potential antibiotic substitutes(ASs)on bacteriostasis,growth performance,intestinal morphology and immunity.Furthermore,the primary,secondary,and tertiary ASs were defined by comparing their results with the results of antibiotics.Results:Among 16,309 identified studies,37 were summarized to study the bacteriostasis effects of feed additives,and 89 were included in the meta-analysis and NMA(10,228 pigs).We summarized 268 associations of 57 interventions with 32 bacteria.The order of bacteriostasis effects was as follows:antimicrobial peptides(AMPs)≈antibiotics>organic acids>plant extracts>oligosaccharides.We detected associations of 11 feed additives and 11 outcomes.Compared with a basal diet,plant extract,AMPs,probiotics,microelements,organic acids,bacteriophages,lysozyme,zymin,and oligosaccharides significantly improved growth performance(P<0.05);organic acids,probiotics,microelements,lysozyme,and AMPs remarkably increased the villus height:crypt depth ratio(V/C)(P<0.05);and plant extracts,zymin,microelements,probiotics,and organic acids notably improved immunity(P<0.05).The optimal AMP,bacteriophage,lysozyme,microelements,oligosaccharides,organic acids,plants,plant extracts,probiotics,and zymin doses were 0.100%,0.150%,0.012%,0.010%,0.050%,0.750%,0.20%,0.040%,0.180%,and 0.100%,respectively.Compared with antibiotics,all investigated feed additives exhibited no significant difference in effects on growth performance,IgG,and diarrhoea index/rate(P>0.05);AMPs and microelements significantly increased V/C(P<0.05);and zymin significantly improved lymphocyte levels(P<0.05).Furthermore,linear weighting sum models were used to comprehensively estimate the overall impact of each feed additive on pig growth and health.Conclusions:Our findings suggest that AMPs and plant extracts can be used as primary ASs for weaned piglets and growing pigs,respectively.Bacteriophages,zymin,plants,probiotics,oligosaccharides,lysozyme,and microelements can be regarded as secondary ASs.Nucleotides and organic acids can be considered as tertiary ASs.Future studies should further assess the alternative effects of combinational feed additives.展开更多
Ongoing challenges in the swine industry,such as reduced access to antibiotics and virus outbreaks(e.g.,porcine epidemic diarrhea virus,African swine fever virus),have prompted calls for innovative feed additives to s...Ongoing challenges in the swine industry,such as reduced access to antibiotics and virus outbreaks(e.g.,porcine epidemic diarrhea virus,African swine fever virus),have prompted calls for innovative feed additives to support pig production.Medium-chain fatty acids(MCFAs)and monoglycerides have emerged as a potential option due to key molecular features and versatile functions,including inhibitory activity against viral and bacterial pathogens.In this review,we summarize recent studies examining the potential of MCFAs and monoglycerides as feed additives to improve pig gut health and to mitigate feed pathogens.The molecular properties and biological functions of MCFAs and monoglycerides are first introduced along with an overview of intervention needs at different stages of pig production.The latest progress in testing MCFAs and monoglycerides as feed additives in pig diets is then presented,and their effects on a wide range of production issues,such as growth performance,pathogenic infections,and gut health,are covered.The utilization of MCFAs and monoglycerides together with other feed additives such as organic acids and probiotics is also described,along with advances in molecular encapsulation and delivery strategies.Finally,we discuss how MCFAs and monoglycerides demonstrate potential for feed pathogen mitigation to curb disease transmission.Looking forward,we envision that MCFAs and monoglycerides may become an important class of feed additives in pig production for gut health improvement and feed pathogen mitigation.展开更多
Effect of UV - B radiation on the feeding behaviour of marine zooplankton is important to assessing the health harm of marine ecosystem due to the gradually enhanced UV - B radiation in air. However, there are a few s...Effect of UV - B radiation on the feeding behaviour of marine zooplankton is important to assessing the health harm of marine ecosystem due to the gradually enhanced UV - B radiation in air. However, there are a few studies on this topic. The feeding behavior of the rotifer, Brachionus plicatilis, under the treatment of UV - B radiation on five species of microalgae, i. e. , Chlorella sp. , Tetraselmis chuii, Isochrysis galbana Park 8701, Chaetoceros muelleri Lermumerman, and Nitzschia clostertum, was studied. The results showed that the filtering and feeding rates of the rotifer decreased significantly with the dose increase of UV - B radiation when fed with five species of microalgae respectively ( P 〈 0.05 ) which indicates UV - B radiation inhibits the feeding activities of the rotifer on microalage. The mixed culture experiments shows the rotifer preferred to feed Chlorella sp. , then C. muelleri, L galbana, N. clostertum and T. chuii in turn if without UV - B radiation. Under the highest dose of UV - B radiation treatment (2.70 kJ/m^2), the rotifer preferred to feed C. muelleri, then Chlorella sp. , N. clostertum, L galbana and T. chuii in turn. Chlorella sp. , L galbana and C. muelleri became the more favorite foods of the rotifer while T. chuii and N. clostertum became less favorite foods. The change of feeding rate and feeding selectivity of zooplankton driven by the enhanced UV - B radiation will lead to the change in the structure of phytoplankton community.展开更多
Based on the structure of chute - feed and autoleveHer, an analysis of their working principle and the verification of their practical production results have been carried out. Finally, the future investigation direet...Based on the structure of chute - feed and autoleveHer, an analysis of their working principle and the verification of their practical production results have been carried out. Finally, the future investigation direetiom of chute - feed and card autuleveller are put forward.展开更多
Naturally occurring plant compounds including tannins, saponins and essential oils are extensively assessed as natural alternatives to in-feed antibiotics.Tannins are a group of polyphenolic compounds that are widely ...Naturally occurring plant compounds including tannins, saponins and essential oils are extensively assessed as natural alternatives to in-feed antibiotics.Tannins are a group of polyphenolic compounds that are widely present in plant region and possess various biological activities including antimicrobial,anti-parasitic, anti-viral, antioxidant, anti-inflammatory, immunomodulation, etc.Therefore, tannins are the major research subject in developing natural alternative to in-feed antibiotics.Strong protein affinity is the well-recognized property of plant tannins, which has successfully been applied to ruminant nutrition to decrease protein degradation in the rumen, and thereby improve protein utilization and animal production efficiency.Incorporations of tannin-containing forage in ruminant diets to control animal pasture bloat, intestinal parasite and pathogenic bacteria load are another 3 important applications of tannins in ruminant animals.Tannins have traditionally been regarded as "anti-nutritional factor" for monogastric animals and poultry, but recent researches have revealed some of them, when applied in appropriate manner, improved intestinal microbial ecosystem, enhanced gut health and hence increased productive performance.The applicability of plant tannins as an alternative to in-feed antibiotics depends on many factors that contribute to the great variability in their observed efficacies.展开更多
基金Financial support was provided by State Key Laboratory of Utilization of Woody Oil Resource(Grant no.2019XK2002)National Natural Science Foundation of China(Grant no.32202004,31972172)+2 种基金Natural Science Foundation of Jiangsu Province(Grant no.BK20201417)Jiangsu Province Research Fund(Grant no.JNHB-131)Starting Research Fund of Yantai University(Grant No.2222006)。
文摘The discovery and application of antibiotics in animal feeds have boomed the development of intensive animal husbandry in the last century,until the emergence of antibiotics-resistant bacteria.To alleviate the risks aroused by antibiotics-resistant bacteria,effective antibiotic substitutes are urgently needed to replace antibiotics.Essential oils(EOs)derived from plants are illustrated as the promising antibiotic substitutes used in animal feeds,as same as their current views for poultry and livestock industries in the future.It has been widely demonstrated that the phytochemicals in EOs show multiple biofunctionability and are less likely to induce resistance in bacteria.The beneficial effects of EOs feed supplementation on the intestinal inflammation,intestinal flora,immunity,digestion,and growth performances have been already extensively examined.However,the cost-effectiveness,odor,volatility,instability and bioavailability are the challenges in effectively utilizing EOs in animal intestines.Based on previous researches,and these challenges can partially be resolved by microencapsulation and nanotechnology are promising techniques to deal with these challenges.This article presents the feasibility and foundation of EOs application as antibiotic substitutes in animal feeds,and illustrates the mechanisms,functional performances and superiority of EOs compared with antibiotics.
基金This project was supported by a grant from Natural Sci-ences Foundation of China(No. 395 70 84 6 )
文摘To study the resistant mechanism and clinical significance of pseudomonas aeruginosa toβ- lactam antibiotics,the outer mem brane permeability rate of30 P.aeruginosa strains to5 β- lactam antibiotics was m easured and their production ofβ- lactamase and theβ- lactam ase genes they carried detected. Furthermore,the relationship between the perm eability,β- lactam ase and the clinical effects ofβ- lactam antibiotics was observed. By using 1 4C- penicillin and liquid- scintillant isotope assay,the affinity of penicillin binding proteins(PBPs) was m easured and their roles in the resistant m echanism studied.Itwas revealed thatthe perm eability rate was higher in sensitive strains than in resistantones(P<0 .0 5 ) .All strains harbored1- 4 β- lactamase genes and produced β- lactam ase.Higher permeability rate and higher degree of stability toβ- lactamase indicated better clinical therapeutic effects. The affinity of PBPs changed little without regard to the perm eability andβ- lactam ase. These results suggested that the permeability of outer mem brane andβ- lacta- mase,but not PBPs,played im portant roles in the resistant mechanism of P. aeruginosa toβ- lac- tam antibiotics and affected the clinical therapeutic effectiveness of som e patients.
文摘Diaper dermatit is among the widely seen inflamatory skin diseases in neonatals and small 0 - 18 month old children. Control of causative agents as well as providing good genital care may prevent or reduce the risk of the disease. The aim of this study is find out the effect of protective genital care on reducing the diaper dermatit development in 0 - 18 months old inpatient 0 - 18 month old children who are using antibiotic. The study was conducted as prospective half-experimetal using control-experimental groups. Our samples comprise of 0 - 18 month old children admitted at Dokuz Eylul University Practical and Research Hospital, pediatric clinic between January and May 2009, 41 0 - 18 month old children as a control group were not under protective genital care, and 42 0 - 18 month old children as an experimental group were under the protecion and using vaseline. The mothers of the experimental group 0 - 18 month old children were given education. All 0 - 18 month old children were followed-up 3 - 7 days and the collected data were evaluated in computer using Pearson’s chisquare, Fisher’s exact chi-square and Yatest-corrected chi-square tests. The diaper dermatit develop- ment ratio was 34.1% in the control group and 7.1% in the experimental group
基金supported by the Key Program of the National Natural Science Foundation of China(3163000269)National Special Fund for Modern Industrial Technology System(CARS-35)Major Science and Technology Special Fund of Zhejiang Province(2015C02022)。
文摘Background:Antibiotic growth promoters are widely used to improve weight gain.However,the abuse of antibiotics can have many negative effects on people.Developing alternatives to antibiotics is an urgent need in livestock production.We aimed to perform a meta-analysis and network meta-analysis(NMA)to investigate the effects of feed additives as potential antibiotic substitutes(ASs)on bacteriostasis,growth performance,intestinal morphology and immunity.Furthermore,the primary,secondary,and tertiary ASs were defined by comparing their results with the results of antibiotics.Results:Among 16,309 identified studies,37 were summarized to study the bacteriostasis effects of feed additives,and 89 were included in the meta-analysis and NMA(10,228 pigs).We summarized 268 associations of 57 interventions with 32 bacteria.The order of bacteriostasis effects was as follows:antimicrobial peptides(AMPs)≈antibiotics>organic acids>plant extracts>oligosaccharides.We detected associations of 11 feed additives and 11 outcomes.Compared with a basal diet,plant extract,AMPs,probiotics,microelements,organic acids,bacteriophages,lysozyme,zymin,and oligosaccharides significantly improved growth performance(P<0.05);organic acids,probiotics,microelements,lysozyme,and AMPs remarkably increased the villus height:crypt depth ratio(V/C)(P<0.05);and plant extracts,zymin,microelements,probiotics,and organic acids notably improved immunity(P<0.05).The optimal AMP,bacteriophage,lysozyme,microelements,oligosaccharides,organic acids,plants,plant extracts,probiotics,and zymin doses were 0.100%,0.150%,0.012%,0.010%,0.050%,0.750%,0.20%,0.040%,0.180%,and 0.100%,respectively.Compared with antibiotics,all investigated feed additives exhibited no significant difference in effects on growth performance,IgG,and diarrhoea index/rate(P>0.05);AMPs and microelements significantly increased V/C(P<0.05);and zymin significantly improved lymphocyte levels(P<0.05).Furthermore,linear weighting sum models were used to comprehensively estimate the overall impact of each feed additive on pig growth and health.Conclusions:Our findings suggest that AMPs and plant extracts can be used as primary ASs for weaned piglets and growing pigs,respectively.Bacteriophages,zymin,plants,probiotics,oligosaccharides,lysozyme,and microelements can be regarded as secondary ASs.Nucleotides and organic acids can be considered as tertiary ASs.Future studies should further assess the alternative effects of combinational feed additives.
文摘Ongoing challenges in the swine industry,such as reduced access to antibiotics and virus outbreaks(e.g.,porcine epidemic diarrhea virus,African swine fever virus),have prompted calls for innovative feed additives to support pig production.Medium-chain fatty acids(MCFAs)and monoglycerides have emerged as a potential option due to key molecular features and versatile functions,including inhibitory activity against viral and bacterial pathogens.In this review,we summarize recent studies examining the potential of MCFAs and monoglycerides as feed additives to improve pig gut health and to mitigate feed pathogens.The molecular properties and biological functions of MCFAs and monoglycerides are first introduced along with an overview of intervention needs at different stages of pig production.The latest progress in testing MCFAs and monoglycerides as feed additives in pig diets is then presented,and their effects on a wide range of production issues,such as growth performance,pathogenic infections,and gut health,are covered.The utilization of MCFAs and monoglycerides together with other feed additives such as organic acids and probiotics is also described,along with advances in molecular encapsulation and delivery strategies.Finally,we discuss how MCFAs and monoglycerides demonstrate potential for feed pathogen mitigation to curb disease transmission.Looking forward,we envision that MCFAs and monoglycerides may become an important class of feed additives in pig production for gut health improvement and feed pathogen mitigation.
基金This work was supported by the National Natural Science Foundation of China under contract Nos 30270258 and 40506028 the National High Technology Research and Development Program of China under contract No. 2002AA648010+2 种基金 the China Postdoctoral Science Foundation under contract No. 2005037237 the Shandong Scientific Research Encouragement Foundation for Talent Youths under contract No. 03BS120the Doctor Scientific Research Foundation of Dongying Vocational College under contract No. B J0602.
文摘Effect of UV - B radiation on the feeding behaviour of marine zooplankton is important to assessing the health harm of marine ecosystem due to the gradually enhanced UV - B radiation in air. However, there are a few studies on this topic. The feeding behavior of the rotifer, Brachionus plicatilis, under the treatment of UV - B radiation on five species of microalgae, i. e. , Chlorella sp. , Tetraselmis chuii, Isochrysis galbana Park 8701, Chaetoceros muelleri Lermumerman, and Nitzschia clostertum, was studied. The results showed that the filtering and feeding rates of the rotifer decreased significantly with the dose increase of UV - B radiation when fed with five species of microalgae respectively ( P 〈 0.05 ) which indicates UV - B radiation inhibits the feeding activities of the rotifer on microalage. The mixed culture experiments shows the rotifer preferred to feed Chlorella sp. , then C. muelleri, L galbana, N. clostertum and T. chuii in turn if without UV - B radiation. Under the highest dose of UV - B radiation treatment (2.70 kJ/m^2), the rotifer preferred to feed C. muelleri, then Chlorella sp. , N. clostertum, L galbana and T. chuii in turn. Chlorella sp. , L galbana and C. muelleri became the more favorite foods of the rotifer while T. chuii and N. clostertum became less favorite foods. The change of feeding rate and feeding selectivity of zooplankton driven by the enhanced UV - B radiation will lead to the change in the structure of phytoplankton community.
文摘Based on the structure of chute - feed and autoleveHer, an analysis of their working principle and the verification of their practical production results have been carried out. Finally, the future investigation direetiom of chute - feed and card autuleveller are put forward.
基金partially funded by Alberta Agriculture and Forestry,Agriculture and Agri-Food Canadaprojects of China Postdoctoral Science Foundation (Grant No.:2017M611930)+1 种基金Natural Science Foundation of Jiangsu Province of China (No.:BK20170494)China Agriculture Research System (CARS-36)
文摘Naturally occurring plant compounds including tannins, saponins and essential oils are extensively assessed as natural alternatives to in-feed antibiotics.Tannins are a group of polyphenolic compounds that are widely present in plant region and possess various biological activities including antimicrobial,anti-parasitic, anti-viral, antioxidant, anti-inflammatory, immunomodulation, etc.Therefore, tannins are the major research subject in developing natural alternative to in-feed antibiotics.Strong protein affinity is the well-recognized property of plant tannins, which has successfully been applied to ruminant nutrition to decrease protein degradation in the rumen, and thereby improve protein utilization and animal production efficiency.Incorporations of tannin-containing forage in ruminant diets to control animal pasture bloat, intestinal parasite and pathogenic bacteria load are another 3 important applications of tannins in ruminant animals.Tannins have traditionally been regarded as "anti-nutritional factor" for monogastric animals and poultry, but recent researches have revealed some of them, when applied in appropriate manner, improved intestinal microbial ecosystem, enhanced gut health and hence increased productive performance.The applicability of plant tannins as an alternative to in-feed antibiotics depends on many factors that contribute to the great variability in their observed efficacies.