Constituents of the anti-asthma herbal formula ASHMI^(TM) synergistically inhibit IL-4 and IL-5 secretion by murine Th2 memory cells,and eotaxin by human lung fibroblasts in vitro

OBJECTIVE： Anti-asthma herbal medicine intervention （ASHMITM）, a combination of three tradi- tional Chinese medicinal herbs developed in our laboratory, has demonstrated efficacy in both mouse models of allergic asthma, and a double-blind placebo-controlled clinical trial in patients with asthma. This study was designed to determine if the anti-inflammatory effects of individual herbal constituents of ASHMITM exhibited synergy. METHODS： Effects of ASHMI and its components aqueous extracts of Lingzhi （Ganoderma lucidum）, Kushen （Sophora flavescens） and Gancao （Glycyrrhiza uralensis）, on Th2 cytokine secretion by murine memory Th2 cells （D10.G4.1） and eotaxin-1 secretion by human lung fibroblast （HLF-1） cells were determined by measuring levels in culture supernatants by enzyme- linked immunosorbent assay. Potential synergistic effects were determined by computing interaction indices from concentration-effect curve parameters. RESULTS： Individual Lingzhi, Kushen and Gancao extracts and ASHMI （the combination of individual extracts） inhibited production of interleukin （IL）-4 and IL-5 by murine memory Th2 cells and eotaxin-1 production by HLF-1 cells. The mean 25%-inhibitory-concentration （IC2s） values （mg/mL） forASHMI, Lingzhi, Kushen and Gancao for IL-4 production were 30.9, 79.4, 123, and 64.6, respectively; for IL-5 production were 30.2, 263, 123.2 and 100, respectively; for eotaxin-1 were 13.2, 16.2, 30.2, and 25.1, respectively. The IC50values （mg/mL） for ASHMI, Lingzhi, Kushen and Gancao for IL-4 production were 158.5, 239.9, 446.7, and 281.8, respectively; for eotaxin-1 were 38.1, 33.1, 100, and 158.5, respectively. The interaction indices of ASHMI constituents at IC25 were 0.35 for IL-4, 0.21 for IL-5 and 0.59 for eotaxin-l. The interaction indices at IC^0 values were 0.50 for IL-4 and 0.62 for eotaxin-1 inhibition. Inhibition of IL-5 did not reach IC^0 values. All interaction indices were below 1 which indicated synergy. CONCLUSION： By comparing the interaction index values, we find that constituents in ASHMITM synergistically inhibited eotaxin-1 production as well as Th2 cytokine production.

Synthesis,Crystal Structure and in vitro Anticancer Studies of 1,2-Bis-benzimidazole-phenyl Copper(Ⅱ) Complex

One new benzimidazole derivative copper（II） complex 1（C10H6Cu N2, Mr = 217.71） has been synthesized and characterized by FT-IR and X-ray single-crystal diffraction. It crystallizes in orthorhombic, space group Pbcn with a = 17.417（2）, b = 8.9963（8）, c = 11.3432（9） ？, V = 1777.3（3） ？3, Z = 8, μ（Mo Kα） = 2.403, F（000） = 872, Dc = 1.627 g/cm3. The final R = 0.0598 for 1153 observed reflections with I 〉 2σ（I） and w R = 0.17 for all data. The in vitro anti-cancer activities of 1, Cu Cl2 and the benzimidazole ligand L were investigated using human cervical（Hela） and hepatocellular carcinoma（Hep-G2） cancer cell lines. The copper（II） complex can greatly inhibit the cell proliferation and show stronger cytotoxic activities against the tested cancer cell lines than both the ligand and copper（II） salt.

Evaluation of in-vitro antibacterial activity and anti-inflammatory activity for different extracts of Rauvolfia tetraphylla L.root bark

Objective:To assess the in-vitro antihacterial activity and anti-inflammatory activity of orally administered different extracts(Hydro-alcoholic,methanolic,ethyl acetate and hexane)of Rauvolfia tetraphylla(R.tetraphylla)root bark in Carrageetiaii induced acute inflammation in rats.Methods:In-vitro antibacterial activity was evaluated for extracts against four Gram positive and four Gram negative bacteria by using cylinder plate assay.Hydro-alcoholic extract(70%v/v ethanol)at 200,400 and 800 mg/kg doses and methanolic,ethyl acetate and hexane extracts at doses 100,200 and 400 mg/kg were tested for anti-inflammatory activity in Carrageenan induced rat paw oedema model and paw thickness was measured every one hour up to 6 hrs.Results:All extracts of R.tetraphylla root bark showed good zone of inhibition against tested bacterial strains.In Carrageenan induced inflammation model,hydro-alcoholic and methanolic extract of R.tetraphylla root bark at three different doses produced significant(P<0.00l)reduction when compared to vehicle treated control group and hexane,ethyl acetate extracts.Conclusions:In the present study extracts of R.tetraphylla root bark shows good in-vitro antibacterial activity and in-vivo anti-inflammatory activity in rats.

In vitro anti-hepatitis B virus activity screening of Gankang granule prescriptions

Objective To improve the compound--Gankang granules(GKGs) on the odor,taste and efficacy by decreasing 30% components and to prepare the new compound GKGs based on the Traditional Chinese Medicine Theory.Methods The drug was extracted by the optimized technology ascertained in the previous studies.The cytotoxicity of the prescriptions was tested by MTT [3-(4,5-di-methylthiazol-2-yl)-2,5-diphenyl tetrazolium] assay,and the anti-hepatitis B virus(anti-HBV) activity was determined by ELISA(enzyme-linked-immunosorbent assay) in vitro.Results In the human HBV-transfected liver cell line HepG2.2.15,the new GKGs did not show any cytotoxicity with the 50% cytotoxic concentration(TC50) of 7.131,1.756 and 1.809 mg/mL after treatment for 3,6 and 9 days respectively,which was obviously higher than that in the human liver cell line HepG2.Moreover,they effectively suppressed the secretion of the HBV antigens with the TI of 8.519,5.730 and 7.066 for HBsAg,and 1.723,12.839 and 47.65 for HBeAg at day 3,6 and 9 respectively.This effect was as good as that of the old GKGs.On the 6th and 9th day,the rate of HBeAg inhibition exceeded 90% even with the concentration as low as 0.16 mg/mL,which was similar to that of the old GKGs.Conclusions These results reflect that the new GKGs precede the old GKGs by much lower cytotoxicity with similar anti-HBV activity,which provides reliable evidences for further pharmacological and toxicological exploration on this new compound.

Anti-Inflammatory Efficacy of Product Containing “Skin Calm Complex” <i>in Vitro</i>Reconstructed Epidermis

Atopic dermatitis is classified as a chronic inflammatory skin disease, which is characterized by alterations in barrier function and immune system of the skin. In a previous study, the efficacy of REPAVAR ATOPIC SKIN BODY CREAM EXTREME (a product containing “SKIN CALM COMPLEX”) on the epidermal barrier structure was demonstrated. However, the product has also been formulated to improve inflammation and itching. The aim of this study is to analyze product effectiveness on skin inflammation and itching which is associated with atopic dermatitis, by quantification of IL-1α and IL-8 interleukins secreted by human keratinocytes from reconstructed epidermis by ELISA assay. Mature (aged 17 days) sample tissues were treated with pro-inflammatory agents (PBS 1X and LPS) and with the product containing synergistic mix from plants extracts and Dihydroavenanthramide D, among other ingredients (“SKIN CALM COMPLEX”) for 24 hours. Measuring the amounts of interleukins by ELISA assay showed 1) decreased levels of IL-1α and 2) no differences about IL-8 secretion. Product REPAVAR ATOPIC SKIN BODY CREAM EXTREME has an anti-inflammatory effect on the release of pro-inflammatory cytokines, becoming an effective preventive agent on inflammation and itching due to maintenance and improvement of the keratinocyte epidermal structure.

Antibacterial, Anti-Inflammatory and Antioxidant Activities of an ACAZY Herbal Formula Used in Traditional Medicine to Treat Respiratory Infections

ACAZY is a plant formula used in traditional medicine in Burkina Faso to treat respiratory infections. After phytochemical analysis, this study evaluated extracts’ anti-inflammatory, antioxidant and antibacterial properties from the ACAZY recipe. Three extractions, an aqueous macerate (AM), an aqueous decoction (AD) and an hydroethanolic macerate (HEM) of the ACAZY recipe powder were carried out. Phytochemical screening of the extracts was carried out using high-performance thin-layer chromatography (HPTLC) and the determination of phenolic compounds. The anti-inflammatory potential was assessed in vitro using pro-inflammatory enzyme inhibition tests. 2,2-Diphenyl-1-picrylhydrazyl (DPPH) and Ferric-reducing antioxidant power (FRAP) antioxidant properties were also determined. The antibacterial activity was evaluated on Staphylococcus aureus and Streptococcus pneumoniae strains. Phytochemical analysis revealed the presence of flavonoids, saponins, tannins, anthracenosids, sterols and triterpenes in the extracts. The extracts inhibited pro-inflammatory enzymes by more than 40% at only 100 µg/mL. The extracts also showed potent antibacterial activity with a minimum inhibitory concentration 1 mg/mL on Staphylococcus aureus and 2 mg/mL on Streptococcus pneumoniae. The extracts in the ACAZY formula have shown anti-inflammatory and antioxidant properties in vitro. The AD also showed an antibacterial activity. This justifies its use in traditional medicine to treat acute respiratory infections.

Hyodeoxycholic acid protects the neurovascular unit against oxygen-glucose deprivation and reoxygenation-induced injury in vitro

Calculus bovis is commonly used for the treatment of stroke in traditional Chinese medicine. Hyodeoxycholic acid(HDCA) is a bioactive compound extracted from calculus bovis. When combined with cholic acid, baicalin and jas-minoidin, HDCA prevents hypoxia-reoxygenation-induced brain injury by suppressing endoplasmic reticulum stress-mediated apoptotic signaling. However, the effects of HDCA in ischemic stroke injury have not yet been studied. Neurovascular unit(NVU) dysfunction occurs in ischemic stroke. Therefore, in this study, we investigated the effects of HDCA on the NVU under ischemic conditions in vitro. We co-cultured primary brain microvascular endothelial cells, neurons and astrocytes using a transwell chamber co-culture system. The NVU was pre-treated with 10.16 or 2.54 μg/mL HDCA for 24 hours before exposure to oxygen-glucose deprivation for 1 hour. The cell counting kit-8 assay was used to detect cell activity. Flow cytometry and terminal deoxynucleotidyl transferase-mediated dUTP nick end labeling were used to assess apoptosis. Enzyme-linked immunosorbent assay was used to measure the expression levels of inflammatory cytokines, including interleukin-1β, interleukin-6 and tumor necrosis factor-α, and neurotrophic factors, including brain-derived neurotrophic factor and glial cell line-derived neurotrophic factor. Oxidative stress-related factors, such as superoxide dismutase, nitric oxide, malondialdehyde and γ-glutamyltransferase, were measured using kits. Pretreatment with HDCA significantly decreased blood-brain barrier permeability and neuronal apoptosis, significantly increased transendothelial electrical resistance and γ-glutamyltransferase activity, attenuated oxidative stress damage and the release of inflammatory cytokines, and increased brain-derived neurotrophic factor and glial cell line-derived neurotrophic factor expression. Our findings suggest that HDCA maintains NVU morphological integrity and function by modulating inflammation, oxidation stress, apoptosis, and the expression of neurotrophic factors. Therefore, HDCA may have therapeutic potential in the clinical management of ischemic stroke. This study was approved by the Ethics Committee of Experimental Animals of Beijing University of Chinese Medicine(approval No. BUCM-3-2016040201-2003) in April 2016.

Anti-diabetic potential of Urena lobata leaf extract through inhibition of dipeptidyl peptidase IV activity

Objective: To evaluate the anti-diabetic potential of leaf extract from Urena lobata(U. lobata) through dipeptidyl peptidase IV(DPP-IV) inhibitory activity.Methods: U. lobata leaf was extracted in hot water and ethanol. The activity of DPPIV inhibitor was tested by in vitro study using gly-pro-p-nitroanilide as substrat of DPPIV and vildagliptin, as standard reference. A product of the reactions between gly-pro-pnitroanilide and DPP-IV, was observed by microplate readers with λ = 405 nm. All data were expressed as mean ± SD and the IC50 value was determined by non linear regression curve fit. Active substances in leaf extract of U. lobata was analyzed by liquid chromatography-mass spectrometry. DPP-IV inhibitory activity of active compounds was evaluated in silico using docking server. Results: The ethanolic extract of U. lobata showed stronger DPP-IV inhibitor activity than water extract with the IC50 values of 1 654.64 and 6 489.88 μg/mL, respectively. Vildagliptin, based on standard reference for DPP-IV inhibitor activity, has IC50 value of 57.44 μg/mL. Based on in silico analysis, mangiferin, stigmasterol and β-sitosterol in U. lobata extract have a strong inhibitory activity on DPP-IV. Conclusions: The results showed that DPP-IV inhibitory activity of U. lobata is related to its active compounds such as mangiferin, stigmasterol and β-sitosterol.

Influence of granulocyte-macrophage colonystimulating factor and tumor necrosis factor on anti-hepatoma activities of human dendritic cells

INTRODUCTIONDendritic cells (DCs) play a key regulatory role inantitumor immunity,especially in its immuneaccessory role via MHC-Ⅰ molecules.We haverecently reported that DCs were able to enhance thekilling activity of Lymphokine and PHA activatedkiller (LPAK) cells in vitro.In the presentstudy,we evaluated the effects of GM-CSF andTNF upon antitumor activities of freshly

Physicochemical properties,antioxidant and anti-inflammatory activities of coumarin-carbonodithioate hybrids

Objective:To study physicochemical properties,antioxidant and anti-inflammatory activities of coumarin-carbonodithioate hybrids.Methods:The substituted 4-bromomethyl coumarins were synthesized in first step by the cyclization.Then the reaction of substituted coumarins(a-e)with potassium O-ethyl/methyl carbonodithioate(1)by using absolute ethanol as solvent,afforded coumarin-carbonodithioate(1a-1j)derivatives under microwave irradiation and the conventional method.The spectroscopic analysis was used for the characterization of coumarin derivatives.The title(1a-1j)compounds were confirmed by spectroscopic methods.Antioxidant property was evaluated by using DPPH free radical-scavenging ability assay method and anti-inflammatory activity was evaluated by protein denaturation procedure using diclofenac sodium as a standard.Drug-likeness.In-silico toxicity was predicted with LD_(50)value and bioactivity score was also calculated for all the compounds.Results:All coumarin(1a-1j)compounds exhibited promisingin-vitro antioxidant and anti-inflammatory properties in comparison to standard drugs.All tested compounds were used for evaluating their physicochemical properties as set by Lipinski rule.It was observed that the synthesized compounds followed rule of five,indicating more'drug-like'nature.Conclusions:All the screened coumarin-carbonodithioates display promising in vitro antioxidant and antiinflammatory activities.From the physicochemical properties of coumarin derivatives,it is found that none of the compounds violate the Lipinski rule and they fall well in the range of rule of five.It is concluded that the coumarin-carbonodithioate hybrids act with more'drug-like'nature.

Cryptoporic acid E from Cryptoporusvolvatus inhibits influenza virus replication in vitro

Influenza virus infection is a global public health issue.The effectiveness of antiviral agents for influenza has been limited by the emergence of drugresistant virus strains.Therefore,there is an urgent need to identify novel antiviral therapies.Our previous studies have found that Cryptoporus volvatus extract could potently inhibit influenza virus replication in vitro and in vivo.However,the effective component of Cryptoporus volvatus which mediated the antiviral activity hasn′t been identified.Here,we identified a novel anti-influenza molecule,cryptoporic acid E(CAE),from Cryptoporus volvatus.Our results showed that CAE had broad-spectrum anti-influenza activity against 2009 pandemic strain A/Beijing/07/2009(H1N1/09),seasonal strain A/Jiangxi/262/05(H3N2),mouse adapted strains A/WSN/33(H1N1)and A/PR8/34(H1N1).We further investigated the mode of CAE action,and found that CAE directlyattenuated influenza virus infectivity.Time-course-analysis indicated that CAE exerted its inhibition mainly at middle stage of the replication cycle of influenza virus.Subsequently,we confirmed that CAE blocked virus RNA replication and transcription in MDCK cells and CAE repressed influenza virus RNA polymerase activity.In addition,we found that CAE impaired influenza virus infectivity by directly targeting virus particles.Our data suggest that CAE is a major effective component of Cryptoporus volvatus and might be a potential candidate for the development of a new anti-influenza virus therapy.

卵巢储备功能低下患者血清AMH、Betatrophin水平与卵巢反应性、IVF-ET妊娠结局关系

目的:探讨卵巢储备功能低下(DOR)患者血清抗苗勒管激素(AMH)、促代谢因子(Betatrophin)水平与卵巢反应性及体外受精-胚胎移植(IVF-ET)妊娠结局的关系。方法:收集2019年12月-2022年12月在本院行IVF-ET的DOR患者137例临床资料,根据卵巢反应性将患者分为低反应组(n=112)、正常反应组(n=16)、高反应组(n=9);根据妊娠结局将患者分为妊娠成功组(n=44)和妊娠失败组(n=93)。酶联免疫吸附法检测血清AMH、Betatrophin水平,受试者工作特性(ROC)曲线评估2项指标预测IVF-ET妊娠结局价值;多因素logistic回归分析影响妊娠的因素。结果:低反应组、正常组、高反应组血清AMH水平(0.49±0.13 ng/ml、0.98±0.21 ng/ml、1.05±0.26 ng/ml)依次升高,血清Betatrophin水平(156.95±16.33 pg/ml、112.17±13.42 pg/ml、92.64±11.03 pg/ml)依次降低;妊娠成功组血清AMH水平(1.07±0.36 ng/ml)高于妊娠失败组(0.34±0.19 ng/ml),Betatrophin水平(136.29±14.42 pg/ml)低于妊娠失败组(216.16±21.05 pg/ml)(均P<0.05)。血清AMH、Betatrophin预测DOR患者IVF-ET妊娠结局的曲线下面积(AUC)(95%CI)别为0.857、0.771,两项指标联合预测的AUC为0.904。多因素logistic回归分析显示,卵泡刺激素/促黄体生成素比值≥2、卵巢低反应、AMH≤0.71 ng/ml、Betatrophin≥176.23 pg/ml是DOR患者IVF-ET妊娠失败的独立危险因素(P<0.05)。结论:血清AMH、Betatrophin水平与DOR患者卵巢反应性、IVF-ET妊娠结局有关,二者有望作为预测DOR患者IVF-ET妊娠结局的生物标记物。

基于体外消化和网络药理学的石榴皮提取物关键抗炎成分及其作用机制探究

自由基与炎症密切相关。为探究石榴皮提取物(PPE)抗炎机理,本试验采用体外模拟消化对PPE进行处理,分析主要成分和自由基清除能力变化,并结合网络药理学和分子对接技术探究PPE核心成分与炎症作用靶点的作用。结果表明,经体外消化后,PPE对羟基自由基(·OH)、2,2-联苯基-1-苦基肼基自由基(DPPH·)、2,2'-联氮-二(3-乙基-苯并噻唑-6-磺酸)二铵盐自由基(ABTS·^(+))的清除能力均较消化前有不同程度提高,其中·OH的半清除浓度(IC_(50·OH))由186.79μg·mL^(-1)下降至119.52μg·mL^(-1),清除效果提高36.01%。通过液质联用技术共分析鉴定出19种化合物,其中6-O-没食子酰葡萄糖苷、表没食子儿茶素、鞣花酸和山奈酚含量在消化后显著提高。网络药理学分析表明,PPE抗炎关键成分主要作用于白介素-1B(IL1B)、白介素-6(IL6)、肿瘤坏死因子(TNF)、基质金属蛋白酶9(MMP9)、前列腺素内过氧化物合酶2(PTGS2)、核因子-κB(NF-κB)、白介素-8(IL8)等7个炎症靶点,与NF-κB炎症通路密切相关。进一步的分子对接结果显示,柯里拉京、鞣花酸、异槲皮苷与PTGS2结合最佳,而山奈酚与MMP9结合最佳。本研究结果可为PPE在抗炎产品开发中的应用提供新思路。

枯草芽孢杆菌源抗菌肽发酵条件优化及其对雏鸡的抗炎作用

研究旨在优化枯草芽孢杆菌源抗菌肽发酵条件并探究其对雏鸡的抗炎作用。试验采用单因素方法筛选出适合枯草芽孢杆菌K4发酵的最佳氮源、碳源及初始pH值、温度、接种量。用筛选出的最佳发酵培养基和条件在50 L发酵罐内发酵1株能产抗菌肽的枯草芽孢杆菌K4,并将最终发酵液添加20%水溶性淀粉进行喷雾干燥。喷干后的菌粉使用大肠杆菌O2作为指示菌进行体外抑菌试验和雏鸡抗炎试验。结果显示,试验筛选出的最佳氮源为蛋白胨,最佳碳源为葡萄糖,培养基初始pH值为7.0,发酵温度为37℃,接种量为5%。喷干后的菌粉活菌数为4.60×10^(10)CFU/g。体外抑菌试验结果显示喷干菌粉对O2的抑菌圈为30 mm。雏鸡抗炎试验结果显示,大肠杆菌感染后的雏鸡灌服抗菌肽K4后的存活率为65%,比药物对照组高18.18%,说明枯草芽孢杆菌K4具有抑制大肠杆菌、降低雏鸡感染大肠杆菌后的死亡率的作用。

维生素E聚乙二醇琥珀酸酯乳化α-生育酚琥珀酸酯纳米乳的制备及体外抗肿瘤细胞活性评价

目的探索以维生素E聚乙二醇琥珀酸酯(TPGS)做乳化剂,制备α-生育酚琥珀酸酯纳米乳(T-NE),对纳米乳进行处方优化、制备方法以及制剂学性质考察,并且评价其体外抗肿瘤细胞活性。方法自2022年1―4月,先后通过水滴定法绘制伪三元相图,根据纳米乳区域确定最优处方,再采用乳化蒸发法制备,并对其进行了理化性质评价,包括类型确定、形态学考察、粒径大小和分布、Zeta电位、载药量和稳定性,并通过细胞活力实验(MTT)进行体外抗肿瘤细胞活性评价。结果T-NE的最优处方为α-生育酚琥珀酸酯(α-TOS)、维生素E、TPGS、聚乙二醇400质量比1∶1∶2∶1;所制得T-NE为O/W型,外观呈类球形且无粘连;载药量为(20.15±1.91)g/L,粒径为(258.8±3.48)nm,多分散指数(PDI)为0.136±0.03,Zeta电位值为(-27.0±0.46)mV;4℃和25℃条件下放置稳定性良好,且适宜室温长期贮存;与游离的α-TOS药物溶液相比,T-NE对人乳腺癌细胞系(MCF-7)及人卵巢癌细胞系(A2780)细胞具有更强的抑制作用,IC50值分别为16.68μmol/L和7.50μmol/L,且空白纳米乳基本无毒性作用。结论该实验制备的T-NE外观均一呈类球形,粒径较小且分布均匀,载药量高稳定性好,同时表现出比游离α-TOS更为优异的抗肿瘤细胞活性作用,具有良好的开发前景。

夫西地酸对分枝杆菌的体外抑菌效果评价

目的:评价夫西地酸(fusidic acid,FA)对分枝杆菌的体外抑菌效果。方法:选取首都医科大学附属北京胸科医院菌株库保存的分枝杆菌标准菌株和临床分离株作为研究菌株,其中,分枝杆菌标准菌株共计45株,包括26株快速生长型分枝杆菌(rapidly growing mycobacteria,RGM)菌株和19株缓慢生长型分枝杆菌(slowly growing mycobacteria,SGM)菌株;分枝杆菌临床分离株共计129株,为首都医科大学附属北京胸科医院2017年1月至2018年12月分离获得,包括结核分枝杆菌(Mycobacterium tuberculosis,MTB)药物敏感株26株、耐多药(multidrug-resistant,MDR)MTB菌株29株、广泛耐药(extensive drug-resistant,XDR)MTB菌株30株、鸟分枝杆菌分离株23株和戈登分枝杆菌分离株21株。采用肉汤稀释法药物敏感性试验,测定FA对研究菌株的最低抑菌浓度(minimum inhibitory concentration,MIC)、MIC_(50)、MIC_(90)、MIC_(99),并将MIC≤16μg/ml的菌株定义为FA敏感株,分析菌株对FA的耐药情况。结果:RGM和SGM标准菌株对FA的耐药率分别为23.1%(6/26)和21.1%(4/19)。FA对MDR-MTB临床分离株的MIC_(50)、MIC_(90)、MIC_(99)分别是4、16、16μg/ml,MTB临床分离株对FA的耐药率为3.5%(3/85),其中MTB药物敏感株、MDR-MTB和XDR-MTB菌株的耐药率分别为0、0和10.0%(3/30)。鸟分枝杆菌和戈登分枝杆菌临床分离株对FA的耐药率分别为17.4%(4/23)和9.5%(2/21)。FA对分枝杆菌临床分离株的MIC_(50)、MIC_(90)、MIC_(99)值分别为4、16、>32μg/ml,分枝杆菌临床分离株对FA的总体耐药率为7.0%(9/129)。结论:FA对分枝杆菌菌种具有较强的抑菌活性,其中对MTB临床分离株具有很好的抑菌效果。

低水平抗苗勒管激素患者体外受精妊娠失败的影响因素分析

目的:探讨低水平抗苗勒管激素(AMH)患者在体外受精/卵胞浆内单精子注射(IVF/ICSI)中妊娠失败的影响因素。方法:回顾性分析于2020年1月1日至2020年12月31日在四川大学华西第二医院生殖医学科接受IVF/ICSI治疗的931例(1184个周期)血清AMH<1.1 ng/ml患者的临床资料。其中503例(525个周期)患者进行了胚胎移植,临床妊娠组193例(193个周期)和临床未妊娠组310例(332个周期);对所有患者根据年龄<35岁、35~<40岁、40~51岁分组,AMH水平<0.06 ng/ml、0.06~<0.20 ng/ml、0.20~<0.50 ng/ml、0.50~<0.80 ng/ml、0.80~<1.10 ng/ml分组,分析年龄、AMH与妊娠结局的关系,采用多因素Logistic回归分析影响低水平AMH患者IVF/ICSI后妊娠失败的相关因素。结果:①与临床妊娠组相比,临床未妊娠组患者的年龄更大,AMH水平和窦卵泡数(AFC)更低,成熟卵母细胞(MⅡ)数、双原核(2PN)受精数、可利用胚胎数、优质胚胎数更少,取卵时子宫内膜厚度更薄,差异有统计学意义(P<0.05)。②不同年龄组间的AMH水平、AFC数、体质量指数(BMI)、促性腺激素(Gn)总量、MⅡ数比较,差异有统计学意义(P<0.05);每移植周期临床妊娠率、每取卵周期临床妊娠率、每促排卵周期临床妊娠率差异也有统计学意义(P<0.001)。③低水平AMH不同范围间的每移植周期临床妊娠率、每取卵周期临床妊娠率和每促排卵周期临床妊娠率的差异有统计学意义(P<0.05)。④多因素Logistic回归分析示,年龄是影响低水平AMH患者胚胎移植后妊娠结局的主要因素(P=0.002)。ROC曲线示,预测临床妊娠的年龄最佳截断值为35.5岁,曲线下面积为0.634(95%CI 0.586~0.682,P<0.001)。结论:对于低水平AMH(<1.1 ng/ml)的患者在IVF/ICSI治疗后仍有妊娠的机会,年龄是影响临床妊娠率的主要因素,而不是AMH的微小差异。患者实际年龄低于35.5岁时,即使AMH水平较低也可能有较好的胚胎移植后临床妊娠结局。

绞股蓝提取物用于护肤品的功效初探

在温度为90℃,m(绞股蓝粉末)∶V(80%乙醇)=1 g∶20 mL,回流2 h条件下,绞股蓝提取率为13.75%,黄酮质量分数为1.34%。绞股蓝提取物在质量浓度达0.04 mg/mL时,清除羟基自由基的能力与VC相近,可达85%;在质量浓度达0.018 mg/mL时,对DPPH自由基清除率达89.64%。绞股蓝提取物对酪氨酸酶活性的抑制率达最高时,质量浓度为600μg/mL,抑制率为57.58%。绞股蓝提取物同1%丙二醇相比,有一定的保湿功效。绞股蓝提取物能有效抑制大肠杆菌、金黄色葡萄球菌,绞股蓝提取物在质量浓度为2.43 mg/mL时有较佳的抗炎效果。对绞股蓝提取物抗氧化、美白保湿、抑菌等方面进行了完整评价,为其开发利用提供了参考。

山橿抗Hp有效组分筛选及其对Hp感染胃癌AGS细胞的影响

目的探究山橿乙酸乙酯部位及其化学成分对幽门螺杆菌(Helicobacter pylori,Hp)的体外抑菌活性,筛选抗Hp有效组分;进一步研究有效组分对Hp感染人胃癌AGS细胞的影响。方法药敏纸片法比较山橿乙酸乙酯部位及其化学成分对Hp的体外抑菌效果,筛选山橿乙酸乙酯部位抗Hp有效成分,组建有效组分;对有效组分及抗Hp最强的化合物,以标准菌株Hp P12、三株多重耐药Hp菌株为实验菌株,克拉霉素为阳性对照药,采用倍比稀释法测定其最低抑菌浓度(MIC)值;细胞实验检测二者对人胃癌AGS细胞增殖、凋亡,Hp感染AGS细胞黏附能力的影响。结果山橿乙酸乙酯部位中二苯乙烯类成分的抗Hp作用优于黄酮类成分,其中银松素的抗Hp作用最为显著;依据山橿乙酸乙酯部位中8个二苯乙烯类成分的含量组建山橿抗Hp有效组分;山橿抗Hp有效组分对Hp标准菌株的MIC值为12~16μg/mL,对Hp耐药菌株的MIC值为256~512μg/mL;银松素对Hp标准菌株的MIC值为16~32μg/mL,对Hp耐药菌株的MIC值为64~128μg/mL,均明显优于山橿乙酸乙酯部位。山橿抗Hp有效组分、银松素对AGS细胞的IC50分别为14.10μg/mL,59.75μg/mL,且二者均不影响AGS细胞凋亡,又可在一定程度上减少Hp的定植。结论山橿乙酸乙酯部位抗Hp的主要有效成分为其中的二苯乙烯类化合物,其中以银松素的作用最强,其抗Hp机制与减少Hp的定植有关。

不同年龄低抗米勒管激素水平助孕患者助孕结局的相关分析

目的分析年龄对抗米勒管激素(AMH)<1.1 ng/mL助孕患者助孕结局的影响。方法选取2020年1月至2022年6月济宁市第一人民医院生殖医学科收治的545例行体外受精/卵胞质内单精子注射-胚胎移植(IVF/ICSI-ET)助孕的低AMH助孕患者作为研究对象。根据年龄分为A组(年龄≤35岁,n=218)、B组(35岁<年龄<40岁,n=139)和C组(年龄≥40岁,n=188)。比较三组的基线资料,以及IVF/ICSI-ET助孕的相关指标和妊娠结局。结果三组不孕年限、体重指数(BMI)、AMH、基础卵泡刺激素(bFSH)、基础黄体生成素(bLH)、基础雌二醇(bE2)、促性腺激素(Gn)用量比较,差异无统计学意义(P>0.05);A组窦卵泡数显著多于B组和C组,差异具有统计学意义(P<0.05);A组、B组Gn天数、穿刺卵泡数及获卵数大于C组,差异具有统计学意义(P<0.05)。三组获卵率、MⅡ卵率、受精率、卵裂率、2PN率、优胚率比较,差异无统计学意义(P>0.05);A组优胚成囊率显著高于B组和C组,差异具有统计学意义(P<0.05);A组、B组、C组的囊胚形成率、可用囊胚率、优胚可用囊胚率依次减少,差异具有统计学意义(P<0.05)。A组、B组、C组的人绒毛膜促性腺激素(HCG)阳性率、临床妊娠率依次下降,自然流产率依次升高,差异无统计学意义(P>0.05),但下降及升高趋势明显。结论在低AMH助孕患者中,低年龄患者较高年龄患者能获得更好的助孕结局,更易取得妊娠成功。