To extend the current understanding of the mercury-mediated cytotoxic effect,five neural cell lines established from different animal species were comparatively analyzed using three different endpoint bioassays：thiaz...To extend the current understanding of the mercury-mediated cytotoxic effect,five neural cell lines established from different animal species were comparatively analyzed using three different endpoint bioassays：thiazolyl blue tetrazolium bromide,3-（4,5-dimethylthiazol-2-yl）-2,5-diphenyl tetrazolium bromide assay（MTT）,neutral red uptake assay（NRU）,and Coomassie blue assay（CB）.Following a 24-hr exposure to selected concentrations of mercury chloride（HgCl_2） and methylmercury（Ⅱ） chloride（MeHgCl）,the cytotoxic effect on test cells was characterized by comparing their 50%inhibition concentration（IC_（50）） values.Experimental results indicated that both these forms of mercury were toxic to all the neural cells,but at very different degrees.The IC_（50）values of MeHgCl among these cell lines ranged from 1.15±0.22 to 10.31 ± 0.70 μmol/L while the IC_（50） values for HgCl_2 were much higher,ranging from 6.44 ± 0.36 to 160.97±19.63 μmol/L,indicating the more toxic nature of MeHgCl.The IC_（50） ratio between HgCl_2and MeHgCl ranged from 1.75 to 96.0,which confirms that organic mercury is much more toxic to these neural cells than inorganic mercury.Among these cell lines,HGST-BR and TriG44 derived from marine sea turtles showed a significantly high tolerance to HgCl_2 as compared to the three mammalian neural cells.Among these neural cells,SK-N-SH represented the most sensitive cells to both chemical forms of mercury.展开更多
基金supported in part by grants from the Centers for Oceans and Human Health(COHH)programthe National Institutes of Environmental Health Sciences(No.P50ES012740)+1 种基金the National Natural Science Foundation of China(Nos:OCE04-32479 and OCE09-11000)Hawaii Community Foundation(14CON-64551)
文摘To extend the current understanding of the mercury-mediated cytotoxic effect,five neural cell lines established from different animal species were comparatively analyzed using three different endpoint bioassays：thiazolyl blue tetrazolium bromide,3-（4,5-dimethylthiazol-2-yl）-2,5-diphenyl tetrazolium bromide assay（MTT）,neutral red uptake assay（NRU）,and Coomassie blue assay（CB）.Following a 24-hr exposure to selected concentrations of mercury chloride（HgCl_2） and methylmercury（Ⅱ） chloride（MeHgCl）,the cytotoxic effect on test cells was characterized by comparing their 50%inhibition concentration（IC_（50）） values.Experimental results indicated that both these forms of mercury were toxic to all the neural cells,but at very different degrees.The IC_（50）values of MeHgCl among these cell lines ranged from 1.15±0.22 to 10.31 ± 0.70 μmol/L while the IC_（50） values for HgCl_2 were much higher,ranging from 6.44 ± 0.36 to 160.97±19.63 μmol/L,indicating the more toxic nature of MeHgCl.The IC_（50） ratio between HgCl_2and MeHgCl ranged from 1.75 to 96.0,which confirms that organic mercury is much more toxic to these neural cells than inorganic mercury.Among these cell lines,HGST-BR and TriG44 derived from marine sea turtles showed a significantly high tolerance to HgCl_2 as compared to the three mammalian neural cells.Among these neural cells,SK-N-SH represented the most sensitive cells to both chemical forms of mercury.
