Tree peony is well known and sought after for its large, colorful flowers. Its propagation is via vegetative methods. Mech- nisms of the adventitious rooting and the regulation of rooting processes are the principles ...Tree peony is well known and sought after for its large, colorful flowers. Its propagation is via vegetative methods. Mech- nisms of the adventitious rooting and the regulation of rooting processes are the principles and techniques of plant propagation and improvement. Microstructures and fluctuations of phytohormones in the adventitious rooting were studied with the etiolated soft- wood shoots of Paeonia suffkuticosa 'Yinfen Jinlin'. There are no pre-primordia in the shoots of the cultivar. Adventitious roots are produced in five stages: shoot selection, primordium initiation, primordium growth, conducting tissue differentiation and root protru- sion. Primordia initiated in the cortex. The contents of the endogenous hormones, IAA, ABA and GA, were 5.842, 0.873 and 1.043 nmol·g^-1 FW on the bases of shoots, respectively. CTKs which included isopentenyl adenine (iPA), zeatin riboside (ZR) and dihy- drozeatin riboside (DHZR) were 0.949, 0.695 and 2.034 nmol·g^-1 FW, respectively. DHZR is active among CTKs. The ratio of IAA to GA, CTK and ABA clearly increased at the stage of primordium initiation, while they showed low levels at the stages of primor- dium growth. The ratios were restored at the shoot levels at the stage of root protrusion. IBA provoked primordia initiation in the cortex, the vascular cambium, the pith and even in the callus induced on the base of shoots. ]AA levels in the treated shoots increased gradually to its highest level (three times of control) at the stage of conducting tissue differentiation. The ratios of IAA to GA, CTK and ABA clearly decreased at the stage of primordium initiation. The ratio of IAA to ABA is regulated at 10:1.展开更多
A study was done to produce a rapid in vitro propagation of three strawberry genotypes and tested in the field under Bangladeshi circumstances. Festival, RABI-3, and Neho strawberry genotypes’ runner tips were cultiv...A study was done to produce a rapid in vitro propagation of three strawberry genotypes and tested in the field under Bangladeshi circumstances. Festival, RABI-3, and Neho strawberry genotypes’ runner tips were cultivated in vitro to induce root induction and multiple shoot proliferation. MS (Murashige and Skoog) media that were basally containing three different concentrations at 1.0, 1.5, and 2.0 of BA (6-benzyl adenine), KIN (6-furfuryl amino purine), or GA<sub>3</sub> (gibberellic acid) at 0.5 mg/L increasing tips of the runner was attained. The culture grew on the medium provided with 1.5 mg/L 6-benzyl adenine and 0.5 mg/L 6-furfuryl amino acid to increase shoot at the best level. Micro-cuttings were rooted on MS media at half strength combined with 0.5 mg/L - 1.5 mg/L IBA (indole butyric acid) or IAA (indole acetic acid). IBA attained 4 - 9 roots and 91% - 96% rooting at 1.0 mg/L. The resulting plantlets grew into hardy plants and took root in the earth. The genotype festival had the highest response rate, followed by RABI-3 and Neho.展开更多
The purpose of the present study was to establish a regeneration procedure for Populus × euramericana 'Neva' by using in vitro shoots tips and leaves. For sterilization, 0.1% (w/v) mercuric chloride (HgCl2)...The purpose of the present study was to establish a regeneration procedure for Populus × euramericana 'Neva' by using in vitro shoots tips and leaves. For sterilization, 0.1% (w/v) mercuric chloride (HgCl2) solution for 8 to 10 min was the optimal treatment for this poplar cultivation. The effects of benzyladenine (BA) and α-naphthaleneacetic acid (NAA) added to Murashige and Skoog (MS) medium were tested on organogenesis. The highest regeneration rate and numbers of shoots/explant from shoot tips (96.7%, 9.8) and leaves (90.0%, 8.7) were obtained on the half-strength MS medium supplemented with 0.5 mg/L BA and 0.1 mg/L NAA. The optimal medium for in vitro rooting of shoots was on a half-strength MS medium containing 1 mg/L indolebutyric acid (IBA) with the highest rooting frequency (93.3%) and numbers of roots/explant (8.2). For acclimatization, in vitro rooted plantlets were transferred to plastic cups containing vermiculite and peat (1: 1). After acclimatization, transplanted plantlets grew well in a shade house. Therefore, we believe that this efficient plant regeneration protocol especially by leaf explants is very important for in vitro clonal propagation of Populus×euramericana 'Neva'.展开更多
A procedure for cutting Prunus humilis(Bge). Sok was comprehensively studied in this paper. It was found that the key factors involved in this procession were medium, rooting accelerator, concentration of rooting ac...A procedure for cutting Prunus humilis(Bge). Sok was comprehensively studied in this paper. It was found that the key factors involved in this procession were medium, rooting accelerator, concentration of rooting accelerator and type of shoot. The results showed that send was used as mediums; Treatment with 1 000 mg/L AST rooting powder No. 2 and semi-woody shoots were the optimal materials for cutting, and the rooting rate reached 88.1%. Anatomical study on rooting of Prunus hum#is(Bge). Sok cutting has been carded out by the paraffin section method. The observation result shows that the adventitious root primordium of Prunus humili$(Bge). Sok cutting belongs to the type of induced root primordium. The adventitiousroot primordium originates from the cross region of vascular cambium and pith rays.展开更多
Eucalyptus is very recalcitrant to in vitro culture.In this research, an efficient shoot organogenesis system was developed using 60-day-old plants of Eucalyptus globulus grown in vitro and non-aerated liquid medium t...Eucalyptus is very recalcitrant to in vitro culture.In this research, an efficient shoot organogenesis system was developed using 60-day-old plants of Eucalyptus globulus grown in vitro and non-aerated liquid medium to improve shoot proliferation. Cultures were initiated with hypocotyls and leaf segments from plantlets cultivated on semisolid 1/2 MS modified medium supplemented with 4.44 μM 6-Benzyladenine(BA) and 16.1 μM 1-Naphthaleneacetic acid(NAA). Calli were transferred to shoot induction medium, with either 0.5 or 2.7 μM NAA. Shoot multiplication was carried out on 4.44 μM BA + 0.5 μM NAA medium, and semisolid and non-aerated liquid systems were compared for improving shoot proliferation.Rooting of adventitious shoots was evaluated on medium containing NAA or Indole-3-butyric acid-IBA(5 and16 μM). Callogenesis was obtained from both types of explants, although shoot formation was only obtained from leaf-derived calli. Shoot proliferation on 4.44 μM BA+0.5 μM NAA resulted in the most shoots/callus.Non-aerated liquid medium was more efficient in promoting shoot multiplication(53.5 shoots/callus) than was semisolid medium(28.5 shoots/callus). Levels of phenolic compounds were significantly reduced in the shoots cultivated in liquid medium. Efficient rooting(76%) was obtained using 16 μM IBA.展开更多
We developed a method for in vitro regenera- tion of Garcinia xanthochymus (yellow mangosteen) from matured seed segments. Multiple shoots were induced on woody plant (WP) medium supplemented with cytokinins. An a...We developed a method for in vitro regenera- tion of Garcinia xanthochymus (yellow mangosteen) from matured seed segments. Multiple shoots were induced on woody plant (WP) medium supplemented with cytokinins. An average of 11 shoots per explant were regenerated from mature seed segments on WP medium containing 20 μM 6-benzylaminopurine. Histological analysis revealed that hypodermal cells of seed segments were initially involved in active division, which later developed into meriste- moids, subsequently leading to the formation of shoot buds. Shoot elongation was achieved by repeated subculturing of seed explants in shoot regeneration medium. Rooting of shoots was achieved on WP medium supplemented with indole-3-butyric acid or s-naphthalene acetic acid. Plant- lets were transplanted to pots containing soil: compost (1:1) and survival rate was 90 %.展开更多
The aim of this study was to investigate the effects of concentration of different growth regulators (auxins and cytokinins) on growth and development of banana shoot tips cultured in vitro. Explants were taken from y...The aim of this study was to investigate the effects of concentration of different growth regulators (auxins and cytokinins) on growth and development of banana shoot tips cultured in vitro. Explants were taken from young suckers of field grown plants of var. “Yangambi”. The shoot tips were cultured on MS media supplemented with different concentrations of BAP (0, 2, 4, 6 and 8 mg/l) with or without IAA at concentration of 0.34 mg/l. At the rooting phase, the media was supplemented with different concentrations of IBA (0.1, 0.5, 1.0, 1.5 and 2.0 mg/l) with or without BAP at concentration of 0.2 mg/l. The results indicated that 6.0 mg/l BAP significantly increased the number of shoots formed and the interaction of 6 mg/l BAP with 0.35 mg/l IAA significantly increased the fresh weight. For rooting, 2.0 mg/l IBA was more efficient in number and length of roots produced than all other treatments.展开更多
The differentiation process including somatic embryogenesis in different Ginkgo explants in vitro culture were studied by cytological observation.The results are as follows:1) two complete cotyledons and a embryo bud ...The differentiation process including somatic embryogenesis in different Ginkgo explants in vitro culture were studied by cytological observation.The results are as follows:1) two complete cotyledons and a embryo bud were observed in mature embryos and several secretory acavitives appeared in maturation region of embryo buds,hypocotyls,cotyledons and radicles after culturing 20 days;two incomplete cotyledons and a embryo bud primordia were found in large cotyledon embryos.The proembryo of two cells,four cells, multi-cellular,and globular embroy were developed from the callus of the small cotyledon embryos.2) The differentiation of cotyledon explants started from epidermal cells,and gradually formed meristematic cell mass in the cortical cells,and eventually adventitious buds were observed.3) The adventitious roots of Ginkgo originated in the cells at the cross of vascular cambium and vascular rays. 4) The type of rooting belongs to induction type by root primordium.The formed adventitious roots were observed after 20 days.展开更多
文摘Tree peony is well known and sought after for its large, colorful flowers. Its propagation is via vegetative methods. Mech- nisms of the adventitious rooting and the regulation of rooting processes are the principles and techniques of plant propagation and improvement. Microstructures and fluctuations of phytohormones in the adventitious rooting were studied with the etiolated soft- wood shoots of Paeonia suffkuticosa 'Yinfen Jinlin'. There are no pre-primordia in the shoots of the cultivar. Adventitious roots are produced in five stages: shoot selection, primordium initiation, primordium growth, conducting tissue differentiation and root protru- sion. Primordia initiated in the cortex. The contents of the endogenous hormones, IAA, ABA and GA, were 5.842, 0.873 and 1.043 nmol·g^-1 FW on the bases of shoots, respectively. CTKs which included isopentenyl adenine (iPA), zeatin riboside (ZR) and dihy- drozeatin riboside (DHZR) were 0.949, 0.695 and 2.034 nmol·g^-1 FW, respectively. DHZR is active among CTKs. The ratio of IAA to GA, CTK and ABA clearly increased at the stage of primordium initiation, while they showed low levels at the stages of primor- dium growth. The ratios were restored at the shoot levels at the stage of root protrusion. IBA provoked primordia initiation in the cortex, the vascular cambium, the pith and even in the callus induced on the base of shoots. ]AA levels in the treated shoots increased gradually to its highest level (three times of control) at the stage of conducting tissue differentiation. The ratios of IAA to GA, CTK and ABA clearly decreased at the stage of primordium initiation. The ratio of IAA to ABA is regulated at 10:1.
文摘A study was done to produce a rapid in vitro propagation of three strawberry genotypes and tested in the field under Bangladeshi circumstances. Festival, RABI-3, and Neho strawberry genotypes’ runner tips were cultivated in vitro to induce root induction and multiple shoot proliferation. MS (Murashige and Skoog) media that were basally containing three different concentrations at 1.0, 1.5, and 2.0 of BA (6-benzyl adenine), KIN (6-furfuryl amino purine), or GA<sub>3</sub> (gibberellic acid) at 0.5 mg/L increasing tips of the runner was attained. The culture grew on the medium provided with 1.5 mg/L 6-benzyl adenine and 0.5 mg/L 6-furfuryl amino acid to increase shoot at the best level. Micro-cuttings were rooted on MS media at half strength combined with 0.5 mg/L - 1.5 mg/L IBA (indole butyric acid) or IAA (indole acetic acid). IBA attained 4 - 9 roots and 91% - 96% rooting at 1.0 mg/L. The resulting plantlets grew into hardy plants and took root in the earth. The genotype festival had the highest response rate, followed by RABI-3 and Neho.
文摘The purpose of the present study was to establish a regeneration procedure for Populus × euramericana 'Neva' by using in vitro shoots tips and leaves. For sterilization, 0.1% (w/v) mercuric chloride (HgCl2) solution for 8 to 10 min was the optimal treatment for this poplar cultivation. The effects of benzyladenine (BA) and α-naphthaleneacetic acid (NAA) added to Murashige and Skoog (MS) medium were tested on organogenesis. The highest regeneration rate and numbers of shoots/explant from shoot tips (96.7%, 9.8) and leaves (90.0%, 8.7) were obtained on the half-strength MS medium supplemented with 0.5 mg/L BA and 0.1 mg/L NAA. The optimal medium for in vitro rooting of shoots was on a half-strength MS medium containing 1 mg/L indolebutyric acid (IBA) with the highest rooting frequency (93.3%) and numbers of roots/explant (8.2). For acclimatization, in vitro rooted plantlets were transferred to plastic cups containing vermiculite and peat (1: 1). After acclimatization, transplanted plantlets grew well in a shade house. Therefore, we believe that this efficient plant regeneration protocol especially by leaf explants is very important for in vitro clonal propagation of Populus×euramericana 'Neva'.
文摘A procedure for cutting Prunus humilis(Bge). Sok was comprehensively studied in this paper. It was found that the key factors involved in this procession were medium, rooting accelerator, concentration of rooting accelerator and type of shoot. The results showed that send was used as mediums; Treatment with 1 000 mg/L AST rooting powder No. 2 and semi-woody shoots were the optimal materials for cutting, and the rooting rate reached 88.1%. Anatomical study on rooting of Prunus hum#is(Bge). Sok cutting has been carded out by the paraffin section method. The observation result shows that the adventitious root primordium of Prunus humili$(Bge). Sok cutting belongs to the type of induced root primordium. The adventitiousroot primordium originates from the cross region of vascular cambium and pith rays.
基金supported by the National Council for Scientific and Technological Development(CNPq)/Brazil,under Grant 477538/2013-4
文摘Eucalyptus is very recalcitrant to in vitro culture.In this research, an efficient shoot organogenesis system was developed using 60-day-old plants of Eucalyptus globulus grown in vitro and non-aerated liquid medium to improve shoot proliferation. Cultures were initiated with hypocotyls and leaf segments from plantlets cultivated on semisolid 1/2 MS modified medium supplemented with 4.44 μM 6-Benzyladenine(BA) and 16.1 μM 1-Naphthaleneacetic acid(NAA). Calli were transferred to shoot induction medium, with either 0.5 or 2.7 μM NAA. Shoot multiplication was carried out on 4.44 μM BA + 0.5 μM NAA medium, and semisolid and non-aerated liquid systems were compared for improving shoot proliferation.Rooting of adventitious shoots was evaluated on medium containing NAA or Indole-3-butyric acid-IBA(5 and16 μM). Callogenesis was obtained from both types of explants, although shoot formation was only obtained from leaf-derived calli. Shoot proliferation on 4.44 μM BA+0.5 μM NAA resulted in the most shoots/callus.Non-aerated liquid medium was more efficient in promoting shoot multiplication(53.5 shoots/callus) than was semisolid medium(28.5 shoots/callus). Levels of phenolic compounds were significantly reduced in the shoots cultivated in liquid medium. Efficient rooting(76%) was obtained using 16 μM IBA.
基金supported by University Grants Commission[Project no.F.No.41-423/2012(SR)]Department of Biotechnology(DBT-KUD-IPLS programme BT/PR14555/INF/22/126/2010)+1 种基金New Delhi and Department of Atomic Energy(BRNS project no.2013/35/BRNS/20)MumbaiIndia
文摘We developed a method for in vitro regenera- tion of Garcinia xanthochymus (yellow mangosteen) from matured seed segments. Multiple shoots were induced on woody plant (WP) medium supplemented with cytokinins. An average of 11 shoots per explant were regenerated from mature seed segments on WP medium containing 20 μM 6-benzylaminopurine. Histological analysis revealed that hypodermal cells of seed segments were initially involved in active division, which later developed into meriste- moids, subsequently leading to the formation of shoot buds. Shoot elongation was achieved by repeated subculturing of seed explants in shoot regeneration medium. Rooting of shoots was achieved on WP medium supplemented with indole-3-butyric acid or s-naphthalene acetic acid. Plant- lets were transplanted to pots containing soil: compost (1:1) and survival rate was 90 %.
文摘The aim of this study was to investigate the effects of concentration of different growth regulators (auxins and cytokinins) on growth and development of banana shoot tips cultured in vitro. Explants were taken from young suckers of field grown plants of var. “Yangambi”. The shoot tips were cultured on MS media supplemented with different concentrations of BAP (0, 2, 4, 6 and 8 mg/l) with or without IAA at concentration of 0.34 mg/l. At the rooting phase, the media was supplemented with different concentrations of IBA (0.1, 0.5, 1.0, 1.5 and 2.0 mg/l) with or without BAP at concentration of 0.2 mg/l. The results indicated that 6.0 mg/l BAP significantly increased the number of shoots formed and the interaction of 6 mg/l BAP with 0.35 mg/l IAA significantly increased the fresh weight. For rooting, 2.0 mg/l IBA was more efficient in number and length of roots produced than all other treatments.
文摘The differentiation process including somatic embryogenesis in different Ginkgo explants in vitro culture were studied by cytological observation.The results are as follows:1) two complete cotyledons and a embryo bud were observed in mature embryos and several secretory acavitives appeared in maturation region of embryo buds,hypocotyls,cotyledons and radicles after culturing 20 days;two incomplete cotyledons and a embryo bud primordia were found in large cotyledon embryos.The proembryo of two cells,four cells, multi-cellular,and globular embroy were developed from the callus of the small cotyledon embryos.2) The differentiation of cotyledon explants started from epidermal cells,and gradually formed meristematic cell mass in the cortical cells,and eventually adventitious buds were observed.3) The adventitious roots of Ginkgo originated in the cells at the cross of vascular cambium and vascular rays. 4) The type of rooting belongs to induction type by root primordium.The formed adventitious roots were observed after 20 days.