长管呼吸器应用于深水导管架施工安全管理

在国家和海洋石油行业“深水战略”背景下,海洋石油行业得到了蓬勃发展,深水海洋石油平台项目不断增多。导管架平台作为应用最广泛的固定式平台,相比于浅水导管架,建造阶段难度更大、风险较高。文章聚焦于建造阶段安全管理,重点分析管腿内受限空间施工作业风险,以及常规通风方式的特点和存在的不足之处。并重点阐述了长管呼吸器替代常规通风模式的工作原理、实际应用效果、应急管理拓展应用及可推广性,以期更好地控制安全风险,保证作业人员安全。

Study of A1298C MTHFR Gene Polymorphism as a Risk Factor for Neural Tube Defects in the Eastern Algerian Population

Background: As C677T mutation, A1298C mutation in methylene tetrahydrofolate reductase (MTHFR) gene results in a decreased MTHFR activity but to a less extent, it is known as a risk factor of predisposition to human neural tube defects (NTDs), in some populations. Our objective was therefore to study, for the first time in Algerian population, if A1298C polymorphism confers risk for the occurrence of this abnormality. We have examined the distribution of the genotype and the allele frequencies of A1298C mutation, and also their influence on plasma homocysteine (Hcy) concentration. Patients and Methods: We studied this polymorphism in 38 mothers of NTD cases and 67 control individuals of an eastern Algerian population. The mutation was determined by polymerase chain reaction-restriction fragment length polymorphism analysis (PCR/RFLP). Plasma homocysteine concentration was analyzed using an automated chemiluminescence method. Results: No signi?cant association could be observed between allele and genotypes frequencies of A1298C MTHFR gene polymorphism and NTDs risk. However, we could observe that A1298C polymorphism affects homocysteine metabolism in mothers of NTD cases leading to homocysteine concentration values higher in AA genotype and lower in AC/CC genotypes (15.29 ± 11.8 μmol/l vs. 8.63 ± 3.83 μmol/l, p < 0.05). Conclusion: Data indicate that A1298C MTHFR gene polymorphism might be a risk factor by affecting homocysteine metabolism in mothers of Algerian children with NTDs.

Inositol (1,4,5)-triphosphate Modulates Pollen Tube Polar Growth

In this article, the functions of D-myo-inositol-1,4,5-trisphosphate (Ins [1,4,5] P3) in regulating pollen tube polar growth were investigated by application of caged version of the phosphoinositides. To increase the intracellular Ins（1,4,5）P3 concentration at the apical region of pollen tube, the caged Ins（1,4,5）P3 loaded by osmotic shock was activated by 10 s 360 nm UV flash at this domain （10 μm from the tip）. More than 70% pollen tubes were induced swelling at apex and/or growth axis reorientation, accompanying temporarily growth arrest, by localized increase of Ins（1,4,5）P3 concentration （n=21）. While, pollen tubes without being loaded with caged Ins（1,4,5）P3 had not response to the same dosage UV flash. With FM 1-43 fluorescent staining, it was found that growth perturbation by UV activated caged Ins（1,4,5）P3 had tight link with membrane trafficking at the apical zone of pollen tubes. Upon UV pulse, the apical V-shaped bright area where was full of secretory vesicles spread to a much broader region, which implied that actin filaments at the apical region were remodeled. It was also observed that the FM 1-43 fluorescence intensity at tip remarkably increased than that before UV flash, which demonstrated that more secretory vesicles were accumulated at this region. To estimate the role of Ins（1,4,5）P3 in modulating intracellular calcium concentration and distribution, dextran conjugated fluorescent dye Calcium Green-1 and Rhodamine B were microinjected into pollen tubes together with caged Ins（1,4,5）P3. The results showed that calcium concentration at the subapical region increased upon UV released Ins（1,4,5）P3. Consequently, the tip-focused calcium gradient under the apical dome of pollen tube was disturbed. Simultaneously, the pollen tube bulged at the apical region and its growth rate decreased. As the tip-focused calcium gradient was reestablished, the pollen tube morphology and growth recovered to the normal level. Therefore, Ins（1,4,5）P3 can modulate pollen tube growth rate and direction through mobilizing intracellular calcium and regulating vesicle trafficking during pollen tube finding its way to the ovary.

对比研究ELISPOT与QFT-GIT对于结核病诊断的应用价值

目的比较ELISPOT和Quanti-FERON-TB Gold In-Tube(QFT-GIT)试剂盒,探讨ELISPOT方法在活动性肺结核诊断中的意义。方法应用ELISPOT和Quanti-FERON-TB Gold In-Tube(QFT-GIT)对105份活动性结核患者(A组)和60份非结核呼吸系统疾病对照组(B组)进行检测。结果 ELISPOT与QFT-GIT方法的敏感度、特异度、阳性预测值及阴性预测值分别为85.71%、86.67%、91.84%及77.61%和83.81%、76.67%、86.27%及73.02%,且两种方法差异无统计学意义(均P>0.05)。结论应于ESAT-6/CFP-10融合蛋白技术的ELISPOT检测方法能够诊断活动性肺结核,并对结核感染诊断提供一定的依据。

螺旋弹簧管的成型工艺研究

螺旋弹簧管是一种使用广泛的传感器零件,其形状对测量精度影响颇大,使用传统的工艺加工该零件时,通常需要额外的工序对焊接端修整。文中修正了基本螺旋线方程,提出了一种新的加工工艺,通过倾斜螺旋线中轴线,倾斜螺旋成圈机构,直接达到使零件的固定端和自由端分别所在的平面平行的目的,不需要二次加工。此方法对螺旋线类零件的加工具有一定的参考意义,可简化工序提高生产效率。

In-Tube SPME-GC/MS测定血浆中丁丙诺啡

目的:建立血浆中丁丙诺啡的毛细管固相微萃取(in-Tube SPME)GC/MS测定方法。方法:将含有丁丙诺啡的血浆经DB-5的毛细管萃取后GC/MS(SIM)分析。结果:血浆中丁丙诺啡回收率为21.2%,检测限为10 ng/ml,相对标准偏差11.2%(n=5)。结论:方法简便、快速,可用于实际临床案例中丁丙诺啡的检测。