Establishing a reliable electrophysiological recording platform is crucial for cardiology and neuroscience research.Noninvasive and label-free planar multitransistors and multielectrode arrays are conducive to perform...Establishing a reliable electrophysiological recording platform is crucial for cardiology and neuroscience research.Noninvasive and label-free planar multitransistors and multielectrode arrays are conducive to perform the large-scale cellular electrical activity recordings,but the signal attenua-tion limits these extracellular devices to record subthreshold activities.In recent decade,in-cell nanoelectronics have been rapidly developed to open the door to intracellular electrophysi-ology.With the unique three-dimensional nanotopography and advanced penetration strategies,high-throughput and high-fidelity action potential like signal recordings is expected to be realized.This review summarizes in-cell nanoelectronics from versatile nano-biointerfaces,penetration strategies,active/pas-sive nanodevices,systematically analyses the applications in electrogenic cells and especially evaluates the influence of nanodevices on the high-quality intracellular electrophysiological signals.Further,the opportunities,challenges and broad prospects of in-cell nanoelectronics are prospected,expecting to promote the development of in-cell electrophysiological platforms to meet the demand of theoretical investigation and clinical application.展开更多
A novel idea of in-cell iR compensation was proposed by using a four-electrode electrochemical system, which was consisted of two working electrodes, one reference electrode （RE） and one auxiliary electrode （AE）. ...A novel idea of in-cell iR compensation was proposed by using a four-electrode electrochemical system, which was consisted of two working electrodes, one reference electrode （RE） and one auxiliary electrode （AE）. One of the two working electrodes was called the auxiliary working electrode （AWE）, which was directly connected to the ground. Another working electrode was used as a regular working electrode （WE） for electrochemical testing. The reference electrode was set in a frit close to the AWE for potential sampling. The other electrodes, WE, RE and AE, were connected to a conventional potentiostat of three-electrode system for electrochemical measurements. A linear narrow electrochemical cell was designed for setting AE at one end and AWE with RE at another end, and setting WE in between AE and AWE. In this way, a positive feedback potential was generated at the working electrode from the solution resistance and the current flow in the solution. An formal iR compensation over 100%, as high as 500%, had been achieved without potential oscillation. The electrochemical cell design, the principle of the in-cell iR compensation, and the preliminary voltammetric characterization by using the redox reaction of ferrocyanide anions were reported.展开更多
基金The work is supported in part by the National Natural Science Foundation of China(Grant Nos.82061148011,61771498)Guangdong Basic and Applied Basic Research Foundation(Grant No.2020A1515010665)+2 种基金Department of Science and Technology of Guangdong Province Project(Grant No.2020B1212060030)Foundation of Sun Yat-sen University(Grant Nos.76120-18821104,20lgpy47,20lgzd14)Open Project of Chinese Academy of Sciences(Grant No.SKT2006).
文摘Establishing a reliable electrophysiological recording platform is crucial for cardiology and neuroscience research.Noninvasive and label-free planar multitransistors and multielectrode arrays are conducive to perform the large-scale cellular electrical activity recordings,but the signal attenua-tion limits these extracellular devices to record subthreshold activities.In recent decade,in-cell nanoelectronics have been rapidly developed to open the door to intracellular electrophysi-ology.With the unique three-dimensional nanotopography and advanced penetration strategies,high-throughput and high-fidelity action potential like signal recordings is expected to be realized.This review summarizes in-cell nanoelectronics from versatile nano-biointerfaces,penetration strategies,active/pas-sive nanodevices,systematically analyses the applications in electrogenic cells and especially evaluates the influence of nanodevices on the high-quality intracellular electrophysiological signals.Further,the opportunities,challenges and broad prospects of in-cell nanoelectronics are prospected,expecting to promote the development of in-cell electrophysiological platforms to meet the demand of theoretical investigation and clinical application.
文摘A novel idea of in-cell iR compensation was proposed by using a four-electrode electrochemical system, which was consisted of two working electrodes, one reference electrode （RE） and one auxiliary electrode （AE）. One of the two working electrodes was called the auxiliary working electrode （AWE）, which was directly connected to the ground. Another working electrode was used as a regular working electrode （WE） for electrochemical testing. The reference electrode was set in a frit close to the AWE for potential sampling. The other electrodes, WE, RE and AE, were connected to a conventional potentiostat of three-electrode system for electrochemical measurements. A linear narrow electrochemical cell was designed for setting AE at one end and AWE with RE at another end, and setting WE in between AE and AWE. In this way, a positive feedback potential was generated at the working electrode from the solution resistance and the current flow in the solution. An formal iR compensation over 100%, as high as 500%, had been achieved without potential oscillation. The electrochemical cell design, the principle of the in-cell iR compensation, and the preliminary voltammetric characterization by using the redox reaction of ferrocyanide anions were reported.
