Free radical scavenging potential and HPTLC analysis of Indigofera tinctoria linn(Fabaceae)

The objective of this study was to evaluate the free radical scavenging potential and high performance thin layer chromatography （HPTLC） fingerprinting of Indigofem tinctoria （I. tinctoria）, Phytochemical analysis was carried out using standard methods, and free radical scavenging activity of the plant was determined using 2,2-diphenyl-l-picrylhydrazy （DPPH）, nitric oxide （NO） and superoxide anion （O2-） radical scavenging capacities. HPTLC plate was kept in CAMAG TLC Scanner 3 and the Rf values at fin- gerprint data were recorded by WlNCATS software, Aqueous extract of I. tinctoria reliably showed the total phenolics （267.2 ± 2.42 mg/g）, flavonoids （75.43 ± 3.36 mg/g） and antioxidants （349.11 ±8.04 mg/g）. The extract was found to have DPPH （52.08%）, NO （23.12%） and 02 （26.79%） scavenging activities at the concentration of 250 pg/mL and the results were statistically significant compared with ascorbic acid standard （p 〈 0.05）. HPTLC results confirmed that the extract contained several potential active com- ponents such as phenols, flavonoids, saponins and terpenoids as the slides revealed multi-colored bands of varying intensities. This study confirmed that the plant had multipotential antioxidant and free ra- dicals scavenging activities.

Comparative and quantitative analysis of antioxidant and scavenging potential of Indigofera tinctoria Linn. extracts

OBJECTIVE：To compare and elucidate the antioxidant effi cacy of ethanolic and hydroethanolic extracts of Indigofera tinctoria Linn.（Fabaceae family）.METHODS：Various in-vitro antioxidant assays and free radical-scavenging assays were done.Quantitative measurements of various phytoconstituents,reductive abilities and chelating potential were carried out along with standard compounds.Half inhibitory concentration（IC50）values for ethanol and hydroethanol extracts were analyzed and compared with respective standards.RESULTS：Hydroethanolic extracts showed considerably more potent antioxidant activity in comparison to ethanol extracts.Hydroethanolic extracts had lower IC50values than ethanol extracts in the case of DPPH,metal chelation and hydroxyl radical-scavenging capacity（829,659 and 26.7μg/m L）but had slightly higher values than ethanol in case of SO2-and NO2-scavenging activity（P〈0.001 vs standard）.Quantitative measurements also showed that the abundance of phenolic and fl avonoid bioactive phytoconstituents were signifi cantly（P〈0.001）greater in hydroethanol extracts（212.920 and 149.770 mg GAE and rutin/g of plant extract respectively）than in ethanol extracts（211.691 and 132.603 mg GAE and rutin/g of plant extract respectively）.K arl Pearson’s co rrelation analysis（r2）between various antioxidant parameters and bioactive components also associated the antioxidant potential of I.tinctoria with various phytoconstituents,especially phenolics,fl avonoids,saponins and tannins.CONCLUSION：This study may be helpful to draw the attention of researchers towards the hydroethanol extracts of I.tinctoria,which has a high yield,and great prospects in herbal industries to produce inexpensive and powerful herbal products.

木蓝和胡枝子对高磷胁迫的生理生态响应及其磷吸收利用特征

通过模拟土壤不同磷水平,测定木蓝和胡枝子对高磷胁迫的生理生态特性、吸收固定能力的响应。结果表明,木蓝的净光合速率随着磷质量浓度的增大逐渐增大,且各处理间有极显著差异,蒸腾速率也随着磷质量浓度的增大而增大,但各处理间无显著差异;胡枝子的净光合速率和蒸腾速率随着磷质量浓度的增加而逐渐减小,其中净光合速率的变化在各处理间差异极显著,而胞间CO2摩尔分数随着磷质量浓度的增加而显著增加。磷质量浓度的变化对2种植物叶片叶绿素质量分数无显著性影响,但是可引起植物体内可溶性糖质量分数在一定范围内产生变化;木蓝对土壤磷的消减程度和植物体内组织的磷质量分数都高于胡枝子,表明木蓝对土壤的吸收和利用能力高于胡枝子。土壤磷质量分数的增加还会引起植物体内组织磷分配与利用的变化。总体而言,木蓝对高磷土壤的适应与吸收利用能力高于胡枝子,更适宜作为先锋物种对磷矿废弃地进行生态恢复。

蓝草类药材的本草考证

该文通过历代本草、医籍、方书的收集整理,结合近现代文献资料对蓝草类药材的名称、基原、产地、药用部位、采收加工及炮制的历史变迁进行了系统的梳理与考证。经考证可知蓝草最早以果实入药即蓝实,基原为蓼科植物蓼蓝Polygonum tinctorium,明清以后蓝实应用较少,至今临床已不再使用;魏晋时期药用部位扩展至茎叶,且多以取汁入药;唐代以来基原扩展至十字花科菘蓝Isatis indigotica、爵床科马蓝(板蓝)Baphicacanthus cusia、豆科木蓝Indigofera tinctoria、菊科植物吴蓝等;宋代扩展至根,始现"板蓝根",并逐步成为蓝类药材的主流药用部位,其主流基原为马蓝B.cusia,自清代将十字花科菘蓝逐步做为了正品板蓝根,而马蓝B.cusia则成为南板蓝根。青黛最早是从国外进口用来画眉的矿物染料,后作药用,又名青蛤粉,因发现其与蓝草的提取物功效一致,而将国产靛花亦命名为青黛,持续至今。大青叶历代主流均为马鞭草科大青属植物大青Clerodendrum cyrtophyllum的干燥茎叶,然自清代以来,逐步将菘蓝Isatis indigotica、蓼蓝P.tinctorium等蓝草的茎叶混入作为替代,并逐步成为主流。