Epithelial-mesenchymal transition （EMT） plays an important role in fibrotic diseases. We have previously showed that silica induces EMT in human bronchial epithelial cells （BECs）; however, the underlying mechanism...Epithelial-mesenchymal transition （EMT） plays an important role in fibrotic diseases. We have previously showed that silica induces EMT in human bronchial epithelial cells （BECs）; however, the underlying mechanism of silica-induced EMT is poorly understood. In the present study, we investigated the role of Snail in silica-induced EMT in human BECs in vitro. Human BECs were treated with silica at various concentrations and incubation times. Then MTr assay, western blot, electrophoretic mobility shift assay （EMSA）, and small interfering RNA （siRNA） transfection were performed. We found that silica increased the expression and DNA binding activity of Snail in human BECs. SNAI silica-induced expression siRNA upregulated the siRNA inhibited the of Snail. Moreover, SNAI expression of epithelial marker E-cadherin, but attenuated the expression of mesenchymal marker a-smooth muscle actin and vimentin in silica-stimulated cells. These results suggest that Snail mediates the silica-induced EMT in human BECs.展开更多
Particulate matter （PM）, which is a great environmental concern, has been classified as a Group 1 human carcinogen by the International Agency for Research on Cancer （IARC）;.Epidemiological and experimental studie...Particulate matter （PM）, which is a great environmental concern, has been classified as a Group 1 human carcinogen by the International Agency for Research on Cancer （IARC）;.Epidemiological and experimental studies have indicated that chronic exposure to PM, especially PM;(particles with an aerodynamic diameter less than展开更多
目的:观察消痛方外敷联合健腰活络针法治疗腰椎术后残余腰背痛的临床疗效。方法:将136例腰椎术后残余腰背痛患者按照随机数字表法分为对照组和观察组各68例。对照组给予洛索洛芬钠片治疗,观察组在对照组基础上给予消痛方外敷联合健腰活...目的:观察消痛方外敷联合健腰活络针法治疗腰椎术后残余腰背痛的临床疗效。方法:将136例腰椎术后残余腰背痛患者按照随机数字表法分为对照组和观察组各68例。对照组给予洛索洛芬钠片治疗,观察组在对照组基础上给予消痛方外敷联合健腰活络针法治疗,7 d为1个疗程,连续治疗2个疗程。比较2组治疗前后疼痛视觉模拟评分法(VAS)评分、腰椎Oswestry功能障碍指数(ODI)、腰椎后伸、前屈活动度、氧化应激指标[丙二醛(MDA)、谷胱甘肽过氧化物酶(GSH-PX)、超氧化物歧化酶(SOD)]及血清中疼痛介质[神经生长因子(NGF)、β-内啡肽(β-EP)、P物质(SP)]含量,并比较2组临床总有效率、不良反应发生率、复发率。结果:治疗7 d、14 d 2组VAS评分均逐渐降低(P<0.05),且同一时间点观察组低于对照组(P<0.05)。治疗后,2组腰椎功能ODI指数均降低(P<0.05),且观察组低于对照组(P<0.05)。治疗后,2组腰椎后伸、前屈活动度均增大(P<0.05),且观察组大于对照组(P<0.05)。治疗后,2组血清MDA水平均降低(P<0.05),且观察组低于对照组(P<0.05);2组血清GSH-PX、SOD水平均升高(P<0.05),且观察组高于对照组(P<0.05)。治疗后,2组NGF、SP水平均降低(P<0.05),且观察组低于对照组(P<0.05);2组β-EP水平均升高(P<0.05),且观察组高于对照组(P<0.05)。治疗后,观察组治疗总有效率95.59%,高于对照组85.29%(P<0.05)。2组不良反应发生率比较,差异无统计学意义(P>0.05)。随访3个月,2组复发率比较,差异无统计学意义(P>0.05)。随访6个月,观察组复发率3.08%,低于对照组15.52%(P<0.05)。结论:消痛方外敷联合健腰活络针法治疗腰椎术后残余腰背痛疗效确切,利于缓解痛感,提高腰椎功能,改善腰椎活动度,抗氧化应激损伤,抑制血清中致痛介质表达,且不良反应少,复发率明显降低。展开更多
基金supported by the National Natural Science Foundation of China(No.30700661,81170023,81470266)China Postdoctoral Science Foundation(2014M562139)Hunan Province Natural Science Foundation(14JJ2041)
文摘Epithelial-mesenchymal transition （EMT） plays an important role in fibrotic diseases. We have previously showed that silica induces EMT in human bronchial epithelial cells （BECs）; however, the underlying mechanism of silica-induced EMT is poorly understood. In the present study, we investigated the role of Snail in silica-induced EMT in human BECs in vitro. Human BECs were treated with silica at various concentrations and incubation times. Then MTr assay, western blot, electrophoretic mobility shift assay （EMSA）, and small interfering RNA （siRNA） transfection were performed. We found that silica increased the expression and DNA binding activity of Snail in human BECs. SNAI silica-induced expression siRNA upregulated the siRNA inhibited the of Snail. Moreover, SNAI expression of epithelial marker E-cadherin, but attenuated the expression of mesenchymal marker a-smooth muscle actin and vimentin in silica-stimulated cells. These results suggest that Snail mediates the silica-induced EMT in human BECs.
基金supported by the National Natural Science Foundation of China[81202231 to LLH]the Medical Scientific Research Funding of Guangdong Province,China[A2018225 to LLH]+1 种基金the College Students Cultivate Special Science and Technology Innovation from Education Department of Guangdong Province,China[pdjh2016a0212]the Project for Creative Talent of Guangdong Education Department[2014KQNCX102]
文摘Particulate matter （PM）, which is a great environmental concern, has been classified as a Group 1 human carcinogen by the International Agency for Research on Cancer （IARC）;.Epidemiological and experimental studies have indicated that chronic exposure to PM, especially PM;(particles with an aerodynamic diameter less than
文摘目的:观察消痛方外敷联合健腰活络针法治疗腰椎术后残余腰背痛的临床疗效。方法:将136例腰椎术后残余腰背痛患者按照随机数字表法分为对照组和观察组各68例。对照组给予洛索洛芬钠片治疗,观察组在对照组基础上给予消痛方外敷联合健腰活络针法治疗,7 d为1个疗程,连续治疗2个疗程。比较2组治疗前后疼痛视觉模拟评分法(VAS)评分、腰椎Oswestry功能障碍指数(ODI)、腰椎后伸、前屈活动度、氧化应激指标[丙二醛(MDA)、谷胱甘肽过氧化物酶(GSH-PX)、超氧化物歧化酶(SOD)]及血清中疼痛介质[神经生长因子(NGF)、β-内啡肽(β-EP)、P物质(SP)]含量,并比较2组临床总有效率、不良反应发生率、复发率。结果:治疗7 d、14 d 2组VAS评分均逐渐降低(P<0.05),且同一时间点观察组低于对照组(P<0.05)。治疗后,2组腰椎功能ODI指数均降低(P<0.05),且观察组低于对照组(P<0.05)。治疗后,2组腰椎后伸、前屈活动度均增大(P<0.05),且观察组大于对照组(P<0.05)。治疗后,2组血清MDA水平均降低(P<0.05),且观察组低于对照组(P<0.05);2组血清GSH-PX、SOD水平均升高(P<0.05),且观察组高于对照组(P<0.05)。治疗后,2组NGF、SP水平均降低(P<0.05),且观察组低于对照组(P<0.05);2组β-EP水平均升高(P<0.05),且观察组高于对照组(P<0.05)。治疗后,观察组治疗总有效率95.59%,高于对照组85.29%(P<0.05)。2组不良反应发生率比较,差异无统计学意义(P>0.05)。随访3个月,2组复发率比较,差异无统计学意义(P>0.05)。随访6个月,观察组复发率3.08%,低于对照组15.52%(P<0.05)。结论:消痛方外敷联合健腰活络针法治疗腰椎术后残余腰背痛疗效确切,利于缓解痛感,提高腰椎功能,改善腰椎活动度,抗氧化应激损伤,抑制血清中致痛介质表达,且不良反应少,复发率明显降低。
