To investigate the impact of different kinds of nerve injuries of early-stage fracture healing.Methods Three groups of rats were included in the experiment among which group 1 was inflicted with femoral fracture and T...To investigate the impact of different kinds of nerve injuries of early-stage fracture healing.Methods Three groups of rats were included in the experiment among which group 1 was inflicted with femoral fracture and T10 spinal cord transsection (SCI),group 2 was inflicted with femoral and peripheral nerve resection (PNR),and group 3 with simple femoral fracture as control group.Two weeks after operation the femoral bones were collected for X-ray checking and 2 more weeks later X-ray checking was performed again followed by pathomorphologic exams.Results X-ray result showed no massive calluses in the bones in the 2nd week postoperatively,while in the 4th week,callus appeared with larger size in group 3 than that of group 1 and with smaller size than that of group 2.It was the same with the result of pathomorphologic examining.Cortical bone bridges between fracture point and osteiod were also found in group 2 and there were less normal blood vessels and worse bone remodeling than that of group 3.There were relatively immature calluses with more fibroblast-like cells and disordered bone structure in group 2.Group 3 showed normal healing process and callus structure.Conclusion Early-stage bone fracture healing can be influenced significantly by different kinds of nerve injuries.6 refs,6 figs.展开更多
Early detection and timely treatment of nerve injury is crucial for the repair of nerve function. One week following a crush injury, heat shock protein 27(HSP27) is over-expressed along the entire length of the sciati...Early detection and timely treatment of nerve injury is crucial for the repair of nerve function. One week following a crush injury, heat shock protein 27(HSP27) is over-expressed along the entire length of the sciatic nerve.Herein, we present an approach to detect injured nerves by photoacoustic microscopy after labeling the injured nerve with HSP27 antibody-conjugated gold nanoparticles. The studies reveal that nanoprobe administration enabled the detection of injured nerves by photoacoustic microscopy, especially during the early stages within3–7 days post injury. In conclusion, photoacoustic microscopy combined with antibody-conjugated nanoparticles holds potential for the early detection of nerve injury.展开更多
文摘To investigate the impact of different kinds of nerve injuries of early-stage fracture healing.Methods Three groups of rats were included in the experiment among which group 1 was inflicted with femoral fracture and T10 spinal cord transsection (SCI),group 2 was inflicted with femoral and peripheral nerve resection (PNR),and group 3 with simple femoral fracture as control group.Two weeks after operation the femoral bones were collected for X-ray checking and 2 more weeks later X-ray checking was performed again followed by pathomorphologic exams.Results X-ray result showed no massive calluses in the bones in the 2nd week postoperatively,while in the 4th week,callus appeared with larger size in group 3 than that of group 1 and with smaller size than that of group 2.It was the same with the result of pathomorphologic examining.Cortical bone bridges between fracture point and osteiod were also found in group 2 and there were less normal blood vessels and worse bone remodeling than that of group 3.There were relatively immature calluses with more fibroblast-like cells and disordered bone structure in group 2.Group 3 showed normal healing process and callus structure.Conclusion Early-stage bone fracture healing can be influenced significantly by different kinds of nerve injuries.6 refs,6 figs.
基金supported by the National Natural Science Foundation of China(No.81271619)the Natural Science Foundation of Guangdong Province(No.10151503102000016)+2 种基金the National High Technology Research and Development Program of China(No.2015AA020901)the Science and Technology Planning Project of Guangdong Province(No.2013B090500122)the Guangdong Natural Science Foundation(No.S2013020012646)
文摘Early detection and timely treatment of nerve injury is crucial for the repair of nerve function. One week following a crush injury, heat shock protein 27(HSP27) is over-expressed along the entire length of the sciatic nerve.Herein, we present an approach to detect injured nerves by photoacoustic microscopy after labeling the injured nerve with HSP27 antibody-conjugated gold nanoparticles. The studies reveal that nanoprobe administration enabled the detection of injured nerves by photoacoustic microscopy, especially during the early stages within3–7 days post injury. In conclusion, photoacoustic microscopy combined with antibody-conjugated nanoparticles holds potential for the early detection of nerve injury.
