In vivo fluorescence has a wide application in analyzing microalgae, including assessing phytoplankton biomass, rates of primary production and physiological status. This study describes a preliminary investigation on...In vivo fluorescence has a wide application in analyzing microalgae, including assessing phytoplankton biomass, rates of primary production and physiological status. This study describes a preliminary investigation on the joint application of the three kinds of fluorescence analysis in the physiological study of microalgae. Flow cytometry and fluorescence spectrometry were used to obtain the in vivo static fluorescence information of pigments, and a Pulsed-Amplitude-Modulation chlorophyll fluorometer was used to detect the dynamic fluorescence of chlorophyll. The validity of the joint application was proved by analyzing two labora- tory cultured Arctic microalgae, Pseudo-nitzschia delicatissima (Bacillariophyceae) and Thalassiosira sp. The higher value of minimum fluorescence yield in dark-adapted state (Fo), actual photochemical efficiency of PSll (ФPSII), and electron transport rate (ETR) exhibited positive results in a higher cell abundance and chlorophyll a content of P. delicatissima; whereas higher fl-carotene content of Thalassiosira sp. played an important role in the protection of photosynthesis.展开更多
This paper suggests effectively integrating national spirits,national ethos and social responsibilities through the inheritance and development direction of traditional Chinese culture to enhance the significance and ...This paper suggests effectively integrating national spirits,national ethos and social responsibilities through the inheritance and development direction of traditional Chinese culture to enhance the significance and national value of traditional Chinese sports culture and proposes scientifically constructing the goals of the development of traditional sports culture with respect to the general characteristics of regional humanity and cultural development,thus building up an innovative development path for the construction of traditional sports culture,satisfying the new requirements for traditional sports culture to develop with the times,and fueling the construction and formation of the new normal of its development.展开更多
基金financially supported by the National Natural Science Foundation of China (Grant no.41076130)the SOA Youth Marine Science Foundation (Grant no.2010116)the Open Research Foundation of Laboratory of Marine Ecosystem and Biogeochemistry,SOA (Grant no.LMEB200902)
文摘In vivo fluorescence has a wide application in analyzing microalgae, including assessing phytoplankton biomass, rates of primary production and physiological status. This study describes a preliminary investigation on the joint application of the three kinds of fluorescence analysis in the physiological study of microalgae. Flow cytometry and fluorescence spectrometry were used to obtain the in vivo static fluorescence information of pigments, and a Pulsed-Amplitude-Modulation chlorophyll fluorometer was used to detect the dynamic fluorescence of chlorophyll. The validity of the joint application was proved by analyzing two labora- tory cultured Arctic microalgae, Pseudo-nitzschia delicatissima (Bacillariophyceae) and Thalassiosira sp. The higher value of minimum fluorescence yield in dark-adapted state (Fo), actual photochemical efficiency of PSll (ФPSII), and electron transport rate (ETR) exhibited positive results in a higher cell abundance and chlorophyll a content of P. delicatissima; whereas higher fl-carotene content of Thalassiosira sp. played an important role in the protection of photosynthesis.
文摘This paper suggests effectively integrating national spirits,national ethos and social responsibilities through the inheritance and development direction of traditional Chinese culture to enhance the significance and national value of traditional Chinese sports culture and proposes scientifically constructing the goals of the development of traditional sports culture with respect to the general characteristics of regional humanity and cultural development,thus building up an innovative development path for the construction of traditional sports culture,satisfying the new requirements for traditional sports culture to develop with the times,and fueling the construction and formation of the new normal of its development.