Effect of Different Sowing Dates and Densities on Individual Morphological Development of Super Short-season Insect-resistant Cotton

[Objective] The paper was to explore the effect of different sowing dates and densities on individual morphological development of super short-season insect-resistant cotton,confirm their effects on vegetative and reproductive growth of cotton,so as to provide theoretical and practical guidance for sowing date and density management of cotton planting in Jidong cotton growing region in Yellow River Basin.[Method] With super short-season insect-resistant cotton＂546＂as materials,the effects of different sowing dates（sowing dateⅠ：May 20;sowing dateⅡ：June 2;sowing date Ⅲ：June 14）and densities（low density：120 000 plants/hm2;middle density：150 000 plants/hm2;high density：180 000 plants/hm2）on individual morphological development of super short-season insect-resistant cotton were explored.[Result] Different sowing dates and density treatments significantly affected the individual morphological development of super short-season insect-resistant cotton＂546＂.The effectiveness of sowing date was higher than the effectiveness of density,and the effectiveness of sowing date on development of number of individual fruit branches was higher than that on plant height and stem diameter.[Conclusion] The regulation of sowing date and density during the cultivation process of super short-season insect-resistant cotton ＂546＂ in Jidong cotton growing region in Yellow River Basin could effectively promote vegetative and reproductive growth of cotton,strengthening its production base.

Study on Insect-resistant Transgenic Cotton Harbouring Double-gene and Its Resistance to Insect Pests

By using the method of pollen tube pathway,the synthesized GFM CryIA gene and modified CpTI gene were transfered into the elite cotton(Gossypium hirsutun L.)varieties(lines).Through the field and lab identifications,the insect-resistant transgenic plants were obtained.PCR analysis indicated that both the synthesized GFM CryIA gene and modified CpTI gene presented positive reaction.In R1 the boliworm resistance of each transformant was different,and the insect-resistance of R3 of ZGK9708 was stable.

Effects of Potassium Fertilizing Rates on Yield, Fiber Quality and Potassium Use Efficiency of Three Hybrid Cotton Cultivars

The effects of potassium （K） application rates on the yield, fiber quality and K fertilizer use efficiency of three hybrid cotton varieties （Jin102, Xiangzamian8, Siyang328） were studied in field experiment. Fertilizer rates of K2O 135 and 270 kg/hm2, representing 1x and 2x recommended K rates, were applied, no application of k fertilizer as the CK. The results show that the lint yield increased 39.13%-57.48%with potassium application, highly significantly. Al yield components of the three hy-brid cotton varieties increased with the increase of K application amounts. The bol number per plant, single bol weight and lint percentage were increased by 14.24%-40.29%, 3.59%-15.51% and 0.16%-4.89%, respectively, and the fiber length and specific strength also increased with the increasing K application amounts, showing no significant influence on Micronaire. When the K application amounts increased from 135 to 270 kg/hm2, the partial factor productivity （PFPk） reduced by 45.93%-48.01%, and the agronomic efficiency （AEk） reduced by 37.1%-42.9%. The PFPk and KE （K efficiency coefficients） of S328 were the highest among the three varieties, which also showed the strongest resistance to low potassium stress, and with no potassi-um fertilizer application （K0）, the lint yield of S328 was 5.54% and 11.19% higher than that of X8 and J102. The AEk of J102 was the highest, and its reward of K fertilizer was the greatest among the three varieties.

Breeding of Transgenic Hybrid Cotton Variety‘Yumian 50' and Cultivation Technology

The mid-early-maturing new cotton variety ＇Yumian 50＇ was bred by In-stitute of Industrial Crops, Henan Academy of Agricultural Sciences. It was exam-ined and approved by Henan Provincial Crop Variety Approval Committee in 2016 （Yushenmian 2016002）. In order to study the variety characteristics of ＇Yumian 50＇ and the key points of its cultivation technology and accelerate its application in cot-ton production, the breeding process of ＇Yumian 50＇ was described in this paper. The test data of 26 times for 3 years of ＇Yumian 50＇ participating in Henan region-al tests in 2012-2014 were summarized. The results showed that ＇Yumian 50＇ had outstanding performance in Henan regional tests and production tests. ＇Yumian 50＇ is a new cotton variety with characteristics of high yield, good quality and high re-sistance to diseases and pests.

Comparisons of Photosynthetic Characteristics in Relation to Lint Yield Among F_1 Hybrids, Their F_2 Descendants and Parental Lines of Cotton

We compared the photosynthetic characteristics in relation to yield of two F1 cotton hybrids（Shiza 2-F1 and Xinluzao 43-F1）, their parental lines（NT2, H2 and 4-14） and their F2 descendants at different growth and development stages. The two F1 exhibited heterobeltiosis in net photosynthetic rate（Pn） by 8.1-52.1%, canopy apparent photosynthetic rate（CAP） by 8.2-57.6% and canopy respiration rate（CR） by 3.0-78.7% during the growing season. They also exhibited mid-parent heterosis by 2.0-5.2% in leaf chlorophyll content（SPAD） during the late growth and development stages. Regression analysis showed that both parents contributed to increase in Pn, SPAD and CAP in the F1. A low CR in the F1 matched a low CR of the parental line. Photosynthetic characteristics in the F2 were mainly dependent upon the magnitude and degeneration rate of the F1. Mid-parent heterosis in CAP and in CR during the late growth and development stage reduced the degeneration of the F2. Average dry matter accumulation was 10.7-34.7% higher in the parental lines of Xinluzao 43-F1 than in the parental lines of Shiza 2-F1. Heterobeltiosis in dry matter accumulation was 7.0-23.1% greater for Xinluzao 43-F1 than for Shiza 2-F1. Dry matter accumulation in the F1 was affected by either the dry matter accumulation of parents or heterobeltiosis. Dry matter accumulation in the F2 was mainly influenced by dry matter accumulation in the F1. The yields of the two F1 were 39.1-46.3% higher than their respective parents and 26.4-45.9% higher than that of the conventional cultivar Xinluzao 33. The yields of the two F2 were 9.2-12.8% higher than the parents and 14.9-27.4% higher than that of Xinluzao 33. The photosynthetic production and yield of the F1 and F2 were higher than that of their parents. The increases in Pn and CAP of the F1 and F2 were dependent on the photosynthetic characteristics of their parents. It is thus concluded that the photosynthetic performance, light use efficiency and yield of the F1 can be improved by using at least one parent with low CR, but high CAP, Pn and SPAD. This strategy might also improve the value of the F2.

The Utilization of HB-red Flower in Hybrid Cotton Breeding

The HB-red flower trait came from the filial generation of the interspecific cross of upland cotton(Gossypium hirsutum L.) and G.bickii.It exhibits pink petals and filaments,with a large

Improvement of Fiber Quality by Distant Hybridization in the Green Cotton

In order to improve fiber quality of green cotton,a wide hybrid was used between a green fiber cotton and Sea Island cotton.The results show that the hybrid F1 plants were stable,but F2

The Cloning and Fluorescence In situ HybridizationAnalysis of Cotton Telomere Sequence

Telomeres form the ends of eukaryotic chromosomes and serve as protective caps that keep chromosomes structure independency and completeness. The first plant telomere DNA was isolated from Arabidopsis thaliana and was shown to have tandemly repeated sequence 5-TTTAGGG-3： The Arabidopsis-type telomere has been found in many plants, but several reports indicate that this sequence is absent in some plants. Up to now, no research has been conducted on the telomere of cotton. In this paper, the Arabidopsis-type telomere sequence was amplified and cloned using the primers designed based on the fragment containing telomere sequence in an Arabidopsis bacterial artificial chromosome （BAC）. Fluorescence in situ hybridization （FISH） with cotton metaphase chromosomes using the Arabidopsis-type telomere sequence as probes indicated that the signals were located at all chromosome ends of seven diploid and two tetraploid cotton species with different signal intensities among chromosome complements of different cotton species, even between long and short arms of the same chromosome. To identify the signals of FISH, the genome DNA of Xinhai 7, a cultivar of Gossypium barbadense, digested by BAL-31 nuclease was introduced in this study. The result of BAL-31 digestion indicated that the hybridization signals of FISH represent the outermost DNA sequence of each cotton chromosomes. So we first proved that the telomeric repeats of cotton cross-hybridize with that of Arabidopsis. The results of terminal restriction fragment （TRF） showed significant variation in telomere length among cotton species. The telomere length of cultivated cotton was close to 20 kb and was larger than those of wild cotton species whose telomere length rahged from 6 to 20 kb.

Fiber Quality Improvement by Interspecific Hybridization in Upland Cotton

In order to transfer genes for extra fiberstrength to existing cotton cultivars,weintroduced 207 interspecific materials obtainedby outcrossing where G. hirsutum,G.barbadense,G.sturtianum,G.thurberi,G.harknessii and G.somalense etc.were involved asparents respectively.Under stringent

Ways of Developing Plants in Interspecific Hybridization of Cotton

It is known,that there are various barriers to fertilization,development of embryos,and endosperm because of different number of chromosomes in parents in the interspecific hybridization of cotton.Thus the factors providing normal cell fission of a germ and endosperm are necessary.It is necessary to culture embryos in vitro on the artificial environments containing various phytohormones,or

Test on Wilt-Resistance of Simple Hybrides F7 and Beckross Hybrid Lines F6 B1 of <i>G. hirsutum</i>L. of Cotton to New Virulent Isolates of Fungus <i>Fusarium verticillioides</i>and <i>Verticillium dahliae</i>

The article presents the results of studies on the resistance of hybrid cotton lines to a new virulent isolate (strain) of the fungus Fusarium verticillioides upon inoculation of the host plant. Based on the studies, it was found that the complex genotypic resistance of the studied lines, when the host plants are inoculated with isolates of -100 V. dahliae Kleb fungus and 103 Fusarium verticillioides fungi, depends on the degree of resistance of the parental forms and their combination ability.

Breeding of Good-quality High-yield Salt-tolerant and Drought-resistant Hybrid Cotton Variety Hengyou 12

Salt and drought tolerance, high yield and quality, disease and pest resistance of cotton varieties are new requirements for the current situation in cotton production. Hengyou 12 is a transgenic Bt cotton variety bred by hybridizing JD 28 （interspeciflc progeny of upland cotton, sea-island cotton, wild cotton species） as female parent with GK55 （transgenic Bt cotton variety） as male parent. The combining of parents was based on the complementary principle of parental traits. Hengyou 12 with strong heterosis was selected under disease stress, pest stress, salt stress and drought stress. This variety has many excellent characteristics, such as good resis- tance to diseases and pests, high yield, excellent quality, strong drought resistance and salt tolerance. ＇Hengyou12＇ was approved by Hebei Province in 2015, and is suitable for planting in middle and south areas of Hebei and similar ecological areas in the Yellow River basin.

Use of Interspecific Hybrids in the Texas A&M University Cotton Improvement Laboratory

Integrating alleles from Gossypium species into G.hirsutum is important for the enhancement of genetic variability and for creating polymorphism useful in molecular mapping studies.Through collaborative efforts,several species including G.barbadense,G.tomentosum,and G.mustelinum

Genotypic Resistance of F_1 Cotton Hybrids by Inoculation with Different Virulent Isolates of the Fungus Verticillium Dahliae Klebahn

The plant pathogen Verticillium dahliae causes severe cotton losses in Uzbekistan. To create cotton varieties that are resistant to the more virulent races of V.dahliae we wanted to determine

Cotton Plants Transformed with the Activated Chimeric Cry1Ac and API-B Genes

A chimeric gene, Bt29K, composed of coding sequences of activated Cry1Ac insecticidal protein and an endoplasm reticulum-retarding signal peptide, was synthesized. A plant expression vector containing two expression cassettes for the Bt29K and API-B genes was constructed. These two insect-resistant genes were transferred into two cotton ( Gossypium hirsutum L.) varieties ( or lines) via Agrobacterium-mediated transformation and nine homozygous transgenic cotton lines showing a mortality of 90.0% - 99.7% to cotton ballworm (Heliothis armigera) larvae and good agronomic traits were selected through six generations. Molecular biology analysis revealed that one or two copies of the insecticidal protein genes were integrated into the transgenic cotton genome and activated Cry1Ac and API-B protein expression was at a level of 0.17% and 0.09% of the total soluble protein in the transgenic cotton leaves, respectively. Comparison of the insect-resistance of the homozygous lines expressing the activated chimeric Cry1Ac and API-B with that expressing Cry1Ac only revealed that the insect-resistance of the former is apparently higher than the latter. These results also indicate that the strategy to construct a plant expression vector expressing two different insect-resistant genes reported here is reasonable.

黄萎病菌诱导下陆地棉酵母双杂交文库的构建及GhMAPKKK2互作蛋白的筛选

为了探究棉花响应黄萎病菌侵染的分子机制并筛选GhMAPKKK2的互作蛋白,以陆地棉XLZ35为材料,基于Gateway克隆技术构建了棉花黄萎病菌诱导的陆地棉酵母双杂交文库,其中初级文库和次级文库的库容分别为1.2×10^(7)和2.0×10^(7)CFU,插入片段重组率均为100%且平均长度大于1000 bp。次级文库质粒浓度为953 ng·μL^(-1),符合构建酵母文库的标准,可用于酵母双杂交筛选互作蛋白。构建了pGBKT7-GhMAPKKK2表达载体,并验证了该载体对酵母细胞无毒性且不存在自激活活性。以GhMAPKKK2为诱饵蛋白,利用酵母文库初步筛选,获得81个阳性互作蛋白,经测序对比选取6个候选蛋白,通过酵母双杂交及双分子荧光互补试验(BiFC)验证,明确了候选蛋白GhFLA与GhMAPKKK2存在互作关系。本研究结果为解析GhMAPKKK2调控棉花抗黄萎病反应分子机制及调控网络提供了重要参考。

Driving Mechanism of Cotton Comber's Detaching Roller Based on Time-Sharing Unidirectional Drive

The high-speed reciprocating motion of a detaching roller limits the velocity of a cotton comber and affects the quality of comber slivers. The article has proposed a controllable time-sharing unidirectional hybrid drive mechanism after analyzing detaching roller's current numerical control drive method. The analysis focuses on the detaching roller motion required according to cotton comber's velocity and process. The double-servo motors of the mechanism consists of differential gear trains. The mechanism addresses the problem of increased servo motor power,and failure of promptly responded to the positive inversion process of mechanism driven by servo motors. A velocity calculation model of the detaching roller controllable drive mechanism will be generated by using superposition method and design of differential gear trains. The accuracy of the model will be verified using the test platform. This study has presented a reliable and practical high-speed drive mechanism and can be a reference to future studies on high-speed reciprocating motion drive.

Genetic analysis of yield and fiber quality traits in upland cotton(Gossypium hirsutum L.) cultivated in different ecological regions of China

Background:Cotton is an important fiber crop worldwide.The yield potential of current genotypes of cotton can be exploited through hybridization.However,to develop superior hybrids with high yield and fiber quality traits,information of genetic control of traits is prerequisite.Therefore,genetic analysis plays pivotal role in plant breeding.Results:In present study,North Carolina II mating design was used to cross 5 female parents with 6 male parents to produce 30 intraspecific F1cotton hybrids.All plant materials were tested in three different ecological regions of China during the year of 2016-2017.Additive-dominance-environment(ADE)genetic model was used to estimate the genetic effects and genotypic and phenotypic correlation of yield and fiber quality traits.Results showed that yield traits except lint percentage were mainly controlled by genetic and environment interaction effects,whereas lint percentage and fiber quality traits were determined by main genetic effects.Moreover,dominant and additiveen vironine nt in teraction effects had more influence on yield traits,whereas additive and domi nance-e nviron ment interaction effects were found to be predominant for fiber traits.Broad-sense and its interaction heritability were significant for all yield and most of fiber quality traits.Narrow-sense and its interaction heritability were non-significant for boll number and seed cotton yield.Correlation analysis indicated that seed cotton yield had significant positive correlation with other yield attributes and non-significant with fiber quality traits.All fiber quality traits had signiflcant positive correlation with each other except micronaire.Conclusions:Results of current study provide important information about genetic control of yield and fiber quality traits.Further,this study identified that parental lines,e.g.,SJ48-1,ZB-1,851-2,and DT-8 can be utilized to improve yield and fiber quality traits in cotton.

Electrospun MgO/Nylon 6 Hybrid Nanofibers for Protective Clothing

Magnesia(MgO) nanoparticles were produced from magnesite ore(MgCO3) using ball mill. The crystalline size, morphology and specific SSA were characterized by X-ray diffraction analysis, transmission electron microscopy and Brunauer-Emmett-Teller method, respectively. MgO nanoparticle-incorporated nylon6 solutions were electrospun to produce nanofiber mats. Surface morphology and internal structure of the prepared hybrid nanofiber mats were examined by scanning electron microscopy and high-resolution transmission electron microscopy, respectively. The fire retardancy and antibacterial activity(Staphylococcus aureus and Escherichia coli) of coated fabrics made from MgO/nylon 6 hybrid nanofiber are better than those from nylon6 nanofiber.