Integrative cultivation practices(ICPs)are essential for enhancing cereal yield and resource use efficiency.However,the effects of ICP on the rhizosphere environment and roots of paddy rice are still poorly understood...Integrative cultivation practices(ICPs)are essential for enhancing cereal yield and resource use efficiency.However,the effects of ICP on the rhizosphere environment and roots of paddy rice are still poorly understood.In this study,four rice varieties were produced in the field.Each variety was treated with six different cultivation techniques,including zero nitrogen application(0 N),local farmers’practice(LFP),nitrogen reduction(NR),and three progressive ICP techniques comprised of enhanced fertilizer N practice and increased plant density(ICP1),a treatment similar to ICP1 but with alternate wetting and moderate drying instead of continuous flooding(ICP2),and the same practices as ICP2 with the application of organic fertilizer(ICP3).The ICPs had greater grain production and nitrogen use efficiency than the other three methods.Root length,dry weight,root diameter,activity of root oxidation,root bleeding rate,zeatin and zeatin riboside compositions,and total organic acids in root exudates were elevated with the introduction of the successive cultivation practices.ICPs enhanced nitrate nitrogen,the activities of urease and invertase,and the diversity of microbes(bacteria)in rhizosphere and non-rhizosphere soil,while reducing the ammonium nitrogen content.The nutrient contents(ammonium nitrogen,total nitrogen,total potassium,total phosphorus,nitrate,and available phosphorus)and urease activity in rhizosphere soil were reduced in all treatments in comparison with the non-rhizosphere soil,but the invertase activity and bacterial diversity were greater.The main root morphology and physiology,and the ammonium nitrogen contents in rhizosphere soil at the primary stages were closely correlated with grain yield and internal nitrogen use efficiency.These findings suggest that the coordinated enhancement of the root system and the environment of the rhizosphere under integrative cultivation approaches may lead to higher rice production.展开更多
A major challenge in rice(Oryza sativa L.)production is to cope with increasing grain yield and fertilizer use efficiency without compromising grain quality.This study was designed to determine if optimizing integrati...A major challenge in rice(Oryza sativa L.)production is to cope with increasing grain yield and fertilizer use efficiency without compromising grain quality.This study was designed to determine if optimizing integrative cultivation management in rice could improve grain quality while increase yield and nitrogen use efficiency(NUE).An indica-japonica hybrid rice cultivar and a japonica rice cultivar were grown in the field,with five cultivation managements including no N application(0 N),local farmer's practice(LFP),and three optimizi ng in teg rati ve cultivati on managements,reducing N rate and increasi ng plant density(ND),ND+alternate wetting and moderate soil drying irrigation(NDW),and NDW+applying rapeseed cake fertilizer(NDWR).The results showed that the optimizi ng integrative cultivati on man ageme nts could not only in crease grain yield,but also enhance NUE compared to LFP.Compared to LFP,NDWR sign ifica ntly in creased brow n,milled,head milled rice rate,ratio of the kern el le ngth to breadth and breakdown value of starch,whereas decreased amylose content,gel consiste ncy,prolamin con tent,setback value,perce ntage of chalky kern els,and chalki ness.The three optimizing in tegrative cultivation managements increased con tents of total protei ns,albumin and glutelin,activities of the key enzymes involved in the sucrose-starch con version in grains,root oxidati on activity,and malic and succinic acid concentrations in root exudates during the grain-filling period.The results suggested that optimizing integrative cultivation managements could improve grain quality meanwhile increase grain yield and NUE by enhancing physiological activities of rice plants.展开更多
基金supported by the National Key Research and Development Program of China (2022YFD2300304)the National Natural Science Foundation of China (32071944 and 32272197)+2 种基金the Hong Kong Research Grants Council, China (GRF 14177617, 12103219, 12103220, and AoE/M-403/16)the State Key Laboratory of Agrobiotechnology (Strategic Collaborative Projects) in The Chinese University of Hong Kong, China, the Six Talent Peaks Project in Jiangsu Province, China (SWYY151)the Project Funded by the Priority Academic Program Development of Jiangsu Higher Education Institutions, China (PAPD).
文摘Integrative cultivation practices(ICPs)are essential for enhancing cereal yield and resource use efficiency.However,the effects of ICP on the rhizosphere environment and roots of paddy rice are still poorly understood.In this study,four rice varieties were produced in the field.Each variety was treated with six different cultivation techniques,including zero nitrogen application(0 N),local farmers’practice(LFP),nitrogen reduction(NR),and three progressive ICP techniques comprised of enhanced fertilizer N practice and increased plant density(ICP1),a treatment similar to ICP1 but with alternate wetting and moderate drying instead of continuous flooding(ICP2),and the same practices as ICP2 with the application of organic fertilizer(ICP3).The ICPs had greater grain production and nitrogen use efficiency than the other three methods.Root length,dry weight,root diameter,activity of root oxidation,root bleeding rate,zeatin and zeatin riboside compositions,and total organic acids in root exudates were elevated with the introduction of the successive cultivation practices.ICPs enhanced nitrate nitrogen,the activities of urease and invertase,and the diversity of microbes(bacteria)in rhizosphere and non-rhizosphere soil,while reducing the ammonium nitrogen content.The nutrient contents(ammonium nitrogen,total nitrogen,total potassium,total phosphorus,nitrate,and available phosphorus)and urease activity in rhizosphere soil were reduced in all treatments in comparison with the non-rhizosphere soil,but the invertase activity and bacterial diversity were greater.The main root morphology and physiology,and the ammonium nitrogen contents in rhizosphere soil at the primary stages were closely correlated with grain yield and internal nitrogen use efficiency.These findings suggest that the coordinated enhancement of the root system and the environment of the rhizosphere under integrative cultivation approaches may lead to higher rice production.
基金supported by the National Basic Research Program of China (2015CB150404)the National Key Research and Development Program of China (2016YFD0300206-4, 2017YFD0301206, 2018YFD0300801)+4 种基金the National Natural Science Foundation of China (31201155, 31871559)the Natural Science Foundation of the Jiangsu Higher Education Department, China (15KJA210005)the Young Elite Scientists Sponsorship Program by China Association for Science and Technology (2016QNRC001)the Six Talent Peaks Project of Jiangsu Province, China (SWYY-151)the Project Funded by the Priority Academic Program Development of Jiangsu Higher Education Institutions, China and the Top Talent Support Plan of Yangzhou University, China (2015-01)
文摘A major challenge in rice(Oryza sativa L.)production is to cope with increasing grain yield and fertilizer use efficiency without compromising grain quality.This study was designed to determine if optimizing integrative cultivation management in rice could improve grain quality while increase yield and nitrogen use efficiency(NUE).An indica-japonica hybrid rice cultivar and a japonica rice cultivar were grown in the field,with five cultivation managements including no N application(0 N),local farmer's practice(LFP),and three optimizi ng in teg rati ve cultivati on managements,reducing N rate and increasi ng plant density(ND),ND+alternate wetting and moderate soil drying irrigation(NDW),and NDW+applying rapeseed cake fertilizer(NDWR).The results showed that the optimizi ng integrative cultivati on man ageme nts could not only in crease grain yield,but also enhance NUE compared to LFP.Compared to LFP,NDWR sign ifica ntly in creased brow n,milled,head milled rice rate,ratio of the kern el le ngth to breadth and breakdown value of starch,whereas decreased amylose content,gel consiste ncy,prolamin con tent,setback value,perce ntage of chalky kern els,and chalki ness.The three optimizing in tegrative cultivation managements increased con tents of total protei ns,albumin and glutelin,activities of the key enzymes involved in the sucrose-starch con version in grains,root oxidati on activity,and malic and succinic acid concentrations in root exudates during the grain-filling period.The results suggested that optimizing integrative cultivation managements could improve grain quality meanwhile increase grain yield and NUE by enhancing physiological activities of rice plants.
