Measurement of mouse liver glutathione S-transferase activity by the integrated method

Objective: The integrated method was investigated to measure Vm/Km of mouse liver glutathione S-transfer-ase (GST) activity on GSH and 7-Cl-4-nitrobenzofurazozan. Methods: Presetting concentration of one substrate twenty-fold above the other's and taking maximum product absorbance Am as parameter while Km as constant, Vm/Km was obtained by nonlinear fitting of GST reaction curve to the integrated Michaelis-Menten equation In [Am/(Am -Ai)] + Ai/ ( ξ× Km ) = ( Vm/Km )×ti (1). Results: Vm/Km for GST showed slight dependence on initial substrate concentration and data range, but it was resistant to background absorbance, error in reaction origin and small deviation in presetting Km. Vm/Km was proportional to the amount of GST with upper limit higher than that by initial rate. There was close correlation between Vm/Km and initial rate of the same GST. Consistent results were obtained by this integrated method and classical initial rate method for the measurement of mouse liver GST. Conclusion: With the concentration of one substrate twenty-fold above the other's, this integrated method was reliable to measure the activity of enzyme on two substrates , and substrate concentration of the lower one close to its apparent Km was able to be used.

Correlation of serum arylesterase activity on phenylacetate estimated by the integrated method to common classical biochemical indexes of liver damage

The correlation of serum arylesterase(PON1) activity on phenylacetate determined by an integrated method to clas-sical biochemical indexes of liver damage was investigated for the use of PON1 activity to evaluate liver damage.PON1 reaction curve as absorbance at 270 nm for 0.20 mmol/L phenylacetate hydrolysis was analyzed by the integrated method to determine maximal PON1 reaction rate.Classical biochemical indexes of liver damage were determined routinely.The 95% confidence threshold of PON1 activity in sera from healthy individuals was 2.12 mkat/L [(4.73±1.31) mkat/L,n=105].PON1 activity in clinical sera was closely correlated to serum albumin,total protein and the ratio of albumin to globulins,but was weakly correlated to both direct and total bilirubin in serum.There were no correlations of PON1 activity to γ-glutamyltransferase,alkaline phos-phatase,alanine aminotransferase and aspartate aminotransferase.Among 127 clinical sera with PON1 activity>2.12 mkat/L,there were 92% healthy individuals examined by albumin,90% healthy individuals examined by total protein,88% healthy individuals examined by total bilirubin,86% healthy individuals examined by direct bilirubin and 64% healthy individuals examined by the ratio of albumin to globulins,respectively.In each group of healthy individuals judged by classical biochemical indexes of close correlation to PON1 activity,percentage of healthy individuals examined by PON1 activity was always >80%.These results suggested PON1 activity on phenylacetate estimated by the integrated method was also suitable for the evaluation of liver damage.

An integrated method for matching forest machinery and a weight-value adjustment

Proper matching of forestry machinery is important when raising mechanization levels for forestry production. In the matching process, forestry machinery needs not only expertise, but also improved methods for solving problems. I propose combination of case-based reasoning （CBR） and rule-based reasoning （RBR） by calculating the similarity of quantitative parameters of various forestry machines in an analytical and hierarchical process. I calculated the similarity of machin-ery used in forest industries to enable better selection and matching of equipment. I propose a weight-value adjusting method based on sums of squares of deviations in which the individual parameter weights were modified in the process of application. During the process of system design, I put forward a design method knowledge base and generated a dynamic web reasoning framework to integrate the processes of forest industry machinery selection and weight-value adjustment. This enables expansion of the scope of the complete system and enhancement of the reasoning efficiency. I demonstrate the validity and practicability of this method using a practical example.

Highly Accurate Golden Section Search Algorithms and Fictitious Time Integration Method for Solving Nonlinear Eigenvalue Problems

This study sets up two new merit functions,which are minimized for the detection of real eigenvalue and complex eigenvalue to address nonlinear eigenvalue problems.For each eigen-parameter the vector variable is solved from a nonhomogeneous linear system obtained by reducing the number of eigen-equation one less,where one of the nonzero components of the eigenvector is normalized to the unit and moves the column containing that component to the right-hand side as a nonzero input vector.1D and 2D golden section search algorithms are employed to minimize the merit functions to locate real and complex eigenvalues.Simultaneously,the real and complex eigenvectors can be computed very accurately.A simpler approach to the nonlinear eigenvalue problems is proposed,which implements a normalization condition for the uniqueness of the eigenvector into the eigenequation directly.The real eigenvalues can be computed by the fictitious time integration method(FTIM),which saves computational costs compared to the one-dimensional golden section search algorithm(1D GSSA).The simpler method is also combined with the Newton iterationmethod,which is convergent very fast.All the proposed methods are easily programmed to compute the eigenvalue and eigenvector with high accuracy and efficiency.

Exponential Time Differencing Method for a Reaction-Diffusion System with Free Boundary

For reaction-diffusion equations in irregular domains with moving boundaries,the numerical stability constraints from the reaction and diffusion terms often require very restricted time step sizes,while complex geometries may lead to difficulties in the accuracy when discretizing the high-order derivatives on grid points near the boundary.It is very challenging to design numerical methods that can efficiently and accurately handle both difficulties.Applying an implicit scheme may be able to remove the stability constraints on the time step,however,it usually requires solving a large global system of nonlinear equations for each time step,and the computational cost could be significant.Integration factor(IF)or exponential time differencing(ETD)methods are one of the popular methods for temporal partial differential equations(PDEs)among many other methods.In our paper,we couple ETD methods with an embedded boundary method to solve a system of reaction-diffusion equations with complex geometries.In particular,we rewrite all ETD schemes into a linear combination of specificФ-functions and apply one state-of-the-art algorithm to compute the matrix-vector multiplications,which offers significant computational advantages with adaptive Krylov subspaces.In addition,we extend this method by incorporating the level set method to solve the free boundary problem.The accuracy,stability,and efficiency of the developed method are demonstrated by numerical examples.

FEA Versus IGA in a Two-Node Beam Element Based on Unified and Integrated Method

This paper presents a new concept called Unified and Integrated Method for a shear deformable beam element.In this method,Timoshenko beam theory is unified and integrated in such a way that takes into account the effect of transverse shear and maintains the shear locking free condition at the same time to generate proper behavior in the analysis of thin to thick beams.The unified and integrated method is applied to finite element analysis(FEA)and isogeometric analysis(IGA)on two-node beam element.This method will be used to analyze uniformly loaded beams with various boundary conditions.A shear influence factor of f,which is a function of beam thickness ratio(L/h),is expressed explicitly as control of the transverse shear strain effect.The analysis gives interesting results showing that applying the unified and integrated method in FEA and IGA will yield exact values of DOF’s and displacement function even when using only a single element.Numerical examples demonstrate the validity and efficiency of the unified and integrated methods.

Integrated method of guidance,control and morphing for hypersonic morphing vehicle in glide phase

The morphing technology of hypersonic vehicle improved the flight performance by changing aerodynamic characteristics with shape deformations,but the design of guidance and control system with morphing laws remained to be explored.An Integrated of Guidance,Control and Morphing(IGCM)method for Hypersonic Morphing Vehicle(HMV)was developed in this paper.The IGCM method contributed to an effective solution of morphing characteristic to improve flight performance and reject the disturbance for guidance and control system caused by the morphing system for HMV in gliding phase.The IGCM models were established based on the motion models and aerodynamic models of the variable span vehicle.Then the IGCM method was designed by adaptive block dynamic surface back-stepping method with stability proof.The parallel controlled simulations’results showed the effectiveness in accomplishing the flight mission of IGCM method in glide phase with smaller terminal errors.The velocity loss of HMV was reduced by 32.8%which inferred less flight time and larger terminal flight velocity than invariable span vehicle.Under the condition of large deviations of aerodynamic parameters and atmospheric density,the robustness of IGCM method with variable span was verified.

Benefit allocation model of distributed photovoltaic power generation vehicle shed and energy storage charging pile based on integrated weighting-Shapley method

In this study,to develop a benefit-allocation model,in-depth analysis of a distributed photovoltaic-powergeneration carport and energy-storage charging-pile project was performed;the model was developed using Shapley integrated-empowerment benefit-distribution method.First,through literature survey and expert interview to identify the risk factors at various stages of the project,a dynamic risk-factor indicator system is developed.Second,to obtain a more meaningful risk-calculation result,the subjective and objective weights are combined,the weights of the risk factors at each stage are determined by the expert scoring method and entropy weight method,and the interest distribution model based on multi-dimensional risk factors is established.Finally,an example is used to verify the rationality of the method for the benefit distribution of the charging-pile project.The results of the example indicate that the limitations of the Shapley method can be reasonably avoided,and the applicability of the model for the benefit distribution of the charging-pile project is verified.

Multiresolution method for bending of plates with complex shapes

A high-accuracy multiresolution method is proposed to solve mechanics problems subject to complex shapes or irregular domains.To realize this method,we design a new wavelet basis function,by which we construct a fifth-order numerical scheme for the approximation of multi-dimensional functions and their multiple integrals defined in complex domains.In the solution of differential equations,various derivatives of the unknown function are denoted as new functions.Then,the integral relations between these functions are applied in terms of wavelet approximation of multiple integrals.Therefore,the original equation with derivatives of various orders can be converted to a system of algebraic equations with discrete nodal values of the highest-order derivative.During the application of the proposed method,boundary conditions can be automatically included in the integration operations,and relevant matrices can be assured to exhibit perfect sparse patterns.As examples,we consider several second-order mathematics problems defined on regular and irregular domains and the fourth-order bending problems of plates with various shapes.By comparing the solutions obtained by the proposed method with the exact solutions,the new multiresolution method is found to have a convergence rate of fifth order.The solution accuracy of this method with only a few hundreds of nodes can be much higher than that of the finite element method(FEM)with tens of thousands of elements.In addition,because the accuracy order for direct approximation of a function using the proposed basis function is also fifth order,we may conclude that the accuracy of the proposed method is almost independent of the equation order and domain complexity.

A model for extracting large deformation mining subsidence using D-InSAR technique and probability integral method

Due to the difficulties in obtaining large deformation mining subsidence using differential Interferometric Synthetic Aperture Radar （D-InSAR） alone, a new algorithm was proposed to extract large deformation mining subsidence using D-InSAR technique and probability integral method. The details of the algorithm are as follows：the control points set, containing correct phase unwrapping points on the subsidence basin edge generated by D-InSAR and several observation points （near the maximum subsidence and inflection points）, was established at first; genetic algorithm （GA） was then used to optimize the parameters of probability integral method; at last, the surface subsidence was deduced according to the optimum parameters. The results of the experiment in Huaibei mining area, China, show that the presented method can generate the correct mining subsidence basin with a few surface observations, and the relative error of maximum subsidence point is about 8.3%, which is much better than that of conventional D-InSAR （relative error is 68.0%）.

A Survey of Mathematical Tools in Topology and Performance Integrated Modeling and Design of Robotic Mechanism

Topology and performance are the two main topics dealt in the development of robotic mechanisms.However,it is still a challenge to connect them by integrating the modeling and design process of both parts in a unified frame.As the properties associated with topology and performance,finite motion and instantaneous motion of the robot play key roles in the procedure.On the purpose of providing a fundamental preparation for integrated modeling and design,this paper carries out a review on the existing unified mathematic frameworks for motion description and computation,involving matrix Lie group and Lie algebra,dual quaternion and pure dual quaternion,finite screw and instantaneous screw.Besides the application in robotics,the review of the work from these mathematicians concentrates on the description,composition and intersection operations of the finite and instantaneous motions,especially on the exponential-differential maps which connect the two sides.Furthermore,an in-depth discussion is worked out by investigating the algebraical relationship among these methods and their further progress in integrated robotic development.The presented review offers insightful investigation to the motion description and computation,and therefore would help designers to choose appropriate mathematical tool in the integrated design and modeling and design of mechanisms and robots.

A new method for integration of a Birkhoffian system

The idea of the gradient method for integrating the dynamical equations of a nonconservative system presented by Vujanovic is transplanted to a Birkhoffian system, which is a new method for the integration of Birkhoff＇s equations. First, the differential equations of motion of the Birkhoffian system are written out. Secondly, 2n Birkhoff＇s variables are divided into two parts, and assume that a part of the variables is the functions of the remaining part of the variables and time. Thereby, the basic quasi-linear partial differential equations are established. If a complete solution of the basic partial differential equations is sought out, the solution of the problem is given by a set of algebraic equations. Since one can choose n arbitrary Birkhoff＇s variables as the functions of n remains of variables and time in a specific problem, the method has flexibility. The major difficulty of this method lies in finding a complete solution of the basic partial differential equation. Once one finds the complete solution, the motion of the systems can be obtained without doing further integration. Finally, two examples are given to illustrate the application of the results.

Method of variation of parameters for solving a constrained Birkhoffian system

For an in-depth study on the integration problem of the constrained mechanical systems the method of integration for the Birkhoffian system with constraints is discussed and the method of variation of parameters for solving the dynamical equations of the constrained Birkhoffian system is provided.First the differential equations of motion for the constrained Birkhoffian system as well as for the corresponding free Birkhoffian system are established.Secondly a system of auxiliary equations is constructed and the general solution of the equations is found.Finally by varying the parameters and utilizing the properties of the generalized canonical transformation of the Birkhoffian system the solution of the problem can be obtained.The proposed method reveals the inherent relationship between the solution of a free Birkhoffian system and that of a constrained Birkhoffian system. The research results are of universal significance which can be further used in a variety of constrained mechanical systems such as non-conservative systems and nonholonomic systems etc.

Fuzzy Inventory Model under Selling Price Dependent Demand and Variable Deterioration with Fully Backlogged Shortages

The objective is to develop a model considering demand dependent on selling price and deterioration occurs after a certain period of time, which follows two-parameter Weibull distribution. Shortages are allowed and fully backlogged. Fuzzy optimal solution is obtained by considering hexagonal fuzzy numbers and for defuzzification Graded Mean Integration Representation Method. A numerical example is provided for the illustration of crisp and fuzzy, both models. To observe the effect of changes in parameters, sensitivity analysis is carried out.

Progress on Therapy of Infertility by Integrated Traditional Chinese and Western Medicine

Infertility is one of the difficult complicated diseases. Many couples suffer from it. The pathogenesis is very complicated. The imbalance or lesson of any link of the reproductive system can cause infertility. This paper summarizes the treatment of female infertility by integrated Traditional Chinese Medicine (TCM) and Western Medicine (WM) which can not only improve the ovulation rate and pregnancy rate, but also decrease the complications. The effects are better than that by TCM or WM only. Therefore, the coupling method is worth to be used widely in clinical practice.

3D elastic wave equation forward modeling based on the precise integration method

The Finite Difference （FD） method is an important method for seismic numerical simulations. It helps us understand regular patterns in seismic wave propagation, analyze seismic attributes, and interpret seismic data. However, because of its discretization, the FD method is only stable under certain conditions. The Arbitrary Difference Precise Integration （ADPI） method is based on the FD method and adopts an integration scheme in the time domain and an arbitrary difference scheme in the space domain. Therefore, the ADPI method is a semi-analytical method. In this paper, we deduce the formula for the ADPI method based on the 3D elastic equation and improve its stability. In forward modeling cases, the ADPI method was implemented in 2D and 3D elastic wave equation forward modeling. Results show that the travel time of the reflected seismic wave is accurate. Compared with the acoustic wave field, the elastic wave field contains more wave types, including PS- and PP- reflected waves, transmitted waves, and diffracted waves, which is important to interpretation of seismic data. The method can be easily applied to elastic wave equation numerical simulations for eoloical models.

Study on Characteristics of 3-D Translating-Pulsating Source Green Function of Deep-Water Havelock Form and Its Fast Integration Method

The singularities, oscillatory performances and the contributing factors to the 3-＇D translating-pulsating source Green function of deep-water Havelock form which consists of a local disturbance part and a far-field wave-like part, are analyzed systematically. Relative numerical integral methods about the two parts are presented in this paper. An improved method based on LOBATTO rule is used to eliminate singularities caused respectively by infinite discontinuity and jump discontinuous node from the local disturbance part function, which makes the improvement of calculation efficiency and accuracy possible. And variable substitution is applied to remove the singularity existing at the end of the integral interval of the far-field wave-like part function. Two auxiliary techniques such as valid interval calculation and local refinement of integral steps technique in narrow zones near false singularities are applied so as to avoid unnecessary integration of invalid interval and improve integral accordance. Numerical test results have proved the efficiency and accuracy in these integral methods that thus can be applied to calculate hydrodynamic performance of floating structures moving in waves.

PARAMETRIC EQUATIONS OF NONHOLONOMIC NONCONSERVATIVE SYSTEMS IN THE EVENT SPACE AND THE METHOD OF THEIR INTEGRATION

In this paper,the parametric equations with multipliers of nonholonomic nonconservative sys- tems in the event space are established,their properties are studied,and their explicit formulation is obtained. And then the field method for integrating these equations is given.Finally,an example illustrating the appli- cation of the integration method is given.

Fast precise integration method for hyperbolic heat conduction problems

A fast precise integration method is developed for the time integral of the hyperbolic heat conduction problem. The wave nature of heat transfer is used to analyze the structure of the matrix exponential, leading to the fact that the matrix exponential is sparse. The presented method employs the sparsity of the matrix exponential to improve the original precise integration method. The merits are that the proposed method is suitable for large hyperbolic heat equations and inherits the accuracy of the original version and the good computational efficiency, which are verified by two numerical examples.

Calibration of Soil Electromagnetic Conductivity in Inverted Salinity Profiles with an Integration Method

Various calibration methods have been propounded to determine profiles of apparent bulk soil electrical conductivity (ECa) and soil electrical conductivity of a saturated soil paste extract (ECe) or a 1:5 soil water extract (EC1:5) using an electromagnetic induction instrument (EM38). The modeled coefficients, one of the successful and classical methods hitherto, were chosen to calibrate the EM38 measurements of the inverted salinity profiles of characteristic coastal saline soils at selected sites of Xincao Farm, Jiangsu Province, China. However, this method required three parameters for each depth layer. An integration approach, based on an exponential decay profile model, was proposed and the model was fitted to all the calibration sites. The obtained model can then be used to predict EC1:5 at a certain depth from electromagnetic measurements made using the EM38 device positioned in horizontal and vertical positions at the soil surface. This exponential decay model predicted the EC1:5 well according to the results of a one-way analysis of variance, and the further comparison indicated that the modeled coefficients appeared to be slightly superior to, but not statistically different from, this exponential decay model. Nevertheless, this exponential decay model was more significant and practical because it depended on less empirical parameters and could be used to perform point predictions of EC1:5 continuously with depth.