Multi‑omics integration identifies regulatory factors underlying bovine subclinical mastitis

Background Mastitis caused by multiple factors remains one of the most common and costly disease of the dairy industry.Multi-omics approaches enable the comprehensive investigation of the complex interactions between mul-tiple layers of information to provide a more holistic view of disease pathogenesis.Therefore,this study investigated the genomic and epigenomic signatures and the possible regulatory mechanisms underlying subclinical mastitis by integrating RNA sequencing data(mRNA and lncRNA),small RNA sequencing data(miRNA)and DNA methylation sequencing data of milk somatic cells from 10 healthy cows and 20 cows with naturally occurring subclinical mastitis caused by Staphylococcus aureus or Staphylococcus chromogenes.Results Functional investigation of the data sets through gene set analysis uncovered 3458 biological process GO terms and 170 KEGG pathways with altered activities during subclinical mastitis,provided further insights into subclin-ical mastitis and revealed the involvement of multi-omics signatures in the altered immune responses and impaired mammary gland productivity during subclinical mastitis.The abundant genomic and epigenomic signatures with sig-nificant alterations related to subclinical mastitis were observed,including 30,846,2552,1276 and 57 differential methylation haplotype blocks(dMHBs),differentially expressed genes(DEGs),lncRNAs(DELs)and miRNAs(DEMs),respectively.Next,5 factors presenting the principal variation of differential multi-omics signatures were identified.The important roles of Factor 1(DEG,DEM and DEL)and Factor 2(dMHB and DEM),in the regulation of immune defense and impaired mammary gland functions during subclinical mastitis were revealed.Each of the omics within Factors 1 and 2 explained about 20%of the source of variation in subclinical mastitis.Also,networks of impor-tant functional gene sets with the involvement of multi-omics signatures were demonstrated,which contributed to a comprehensive view of the possible regulatory mechanisms underlying subclinical mastitis.Furthermore,multi-omics integration enabled the association of the epigenomic regulatory factors(dMHBs,DELs and DEMs)of altered genes in important pathways,such as‘Staphylococcus aureus infection pathway’and‘natural killer cell mediated cyto-toxicity pathway’,etc.,which provides further insights into mastitis regulatory mechanisms.Moreover,few multi-omics signatures(14 dMHBs,25 DEGs,18 DELs and 5 DEMs)were identified as candidate discriminant signatures with capac-ity of distinguishing subclinical mastitis cows from healthy cows.Conclusion The integration of genomic and epigenomic data by multi-omics approaches in this study provided a better understanding of the molecular mechanisms underlying subclinical mastitis and identified multi-omics candidate discriminant signatures for subclinical mastitis,which may ultimately lead to the development of more effective mastitis control and management strategies.

System integration and innovation of operating 20 kt combined heavy-haul train on Datong-Qinhuangdao line

Datong-Qinhuangdao line, as the first electrified double-track heavy-haul line dedicated to coal transport in China, extends from Datong in the west, and reaches Qinhuangdao in the east, opened to traffic in December, 1992, totaling 653 km with the designed annual traffic volume of 1×108 t. In order to meet the demands of national economic development, the transport capacity of the line must be enhanced greatly. Depending on independent innovation, MOR, for the first time in the world,realizes the integration between GSM-R and Locotrol, the integration between 800 MHz digital radio and Locotrol, and the integration between a single set of Locotrol and SS4 locomotive. Meanwhile, CR develops equipment portfolio for heavy-haul through combining 2 high power locomotives of HXD series (means harmony) with controllable EOT. Relying on integration and innovation, it succeeds in operating 20 kt-level combined heavy-haul train on Datong-Qinhuangdao line, which tripled the annual traffic volume of the line from 1×108 t in 2002 to 3×108 t in 2007.

Numerical Solution of Two-Dimensional Nonlinear Stochastic Ito-Volterra Integral Equations by Applying Block Pulse Functions

This paper investigates the numerical solution of two-dimensional nonlinear stochastic Itô-Volterra integral equations based on block pulse functions. The nonlinear stochastic integral equation is transformed into a set of algebraic equations by operational matrix of block pulse functions. Then, we give error analysis and prove that the rate of convergence of this method is efficient. Lastly, a numerical example is given to confirm the method.

THE PROBLEM OF PARAMETRIC INTEGRATION TO THE SLIP LINE FIELD IN THE COMPRESSION OF THICK WORKPIECE

An integration depending on a parameter to the compression of a thick workpiece has been obtained. For the conventional prevailing numerical formulaa definite functional relationship bet-ween and y is found. Therefore a parametric integration can be used to get an analytical solution. Take the slip line field for l/h= 0.121 as an example, the analytical solution is basically the same as the prevailing numerical one. It is justified theoretically that for the slip line field a parametric integration is perfectly possible for a satisfactory analytical solution.

Integration of three-dimensional continuum model and two-dimensional bonded block model for studying the damage process in a granite pillar at the Creighton Mine,Sudbury,Canada

Bonded blockmodel(BBM)has shownpotential in replicating rockmass behavior aswell as the rockesupport interactionmechanism,but their practical application is limited totwo dimensions due to the high associated computational demand.To allow for the use of BBM in simulating three-dimensional(3D)problems,this study proposes an integrated 3D continuumetwo-dimensional(2D)discontinuum approach,in context of rock pillars.A cross-section of a granite pillar was simulated using a BBM with a load path from a calibrated mine-scale FLAC^(3D)model.Pillar support as employed in the mine was also incorporated in different stages during the simulation.Themodel was calibrated by varying the input parameters until the displacements at six locations within the pillarmatchedthosemeasuredby amulti-point borehole extensometer(MPBX)inthe field.The calibratedmodel was subsequently used to understand how the support and load path influenced the damage evolution in the pillar.The shear component of the load pathwas found to have amajor effect on the severity and extent of the damaged regions.When the support density was increased in the model,the lateral displacements along the pillar walls were significantly suppressed in a somewhat unpredictable manner.Thiswas explained by the interaction between the supports and the damaged regions at the corners,which ultimately modified the stresses along the pillar periphery.The amount of displacement reduction obtained by increasing the support density illustrates the potential of BBMto be used as a support design tool.

Runge-Kutta Method and Bolck by Block Method to Solve Nonlinear Fredholm-Volterra Integral Equation with Continuous Kernel

In this paper, the existence and uniqueness of the solution of Fredholm-Volterra integral equation is considered (NF-VIE) with continuous kernel;then we used a numerical method to reduce this type of equations to a system of nonlinear Volterra integral equations. Runge-Kutta method (RKM) and Bolck by block method (BBM) are used to solve the system of nonlinear Volterra integral equations of the second kind (SNVIEs) with continuous kernel. The error in each case is calculated.

Implementation of Dynamic Line Rating in a Sub-Transmission System for Wind Power Integration

Based on conventional static line rating method, the actual current carrying capability of overhead conductors cannot be judged. Due to continuous increment in electricity demand and the difficulties associated with new line constructions, the overhead lines are therefore required to be rated based on a method that should establish their real-time capability in terms of electricity transmission. The method used to determine the real-time ampacity of overhead conductors not only can enhance their transmission capacity but can also help in allowing excessive renewable generation in the electricity network. In this research work, the issues related to analyzing an impact of wind power on periodical loading of overhead line as well as finding its static and dynamic ampacities with line current are investigated in detail. Moreover, the investigation related to finding a suitable location for the construction of a 60 MW wind farm is taken on board. Thereafter, the wind park is integrated with a regional grid, owned by Fortum Distribution AB. In addition to that, the electricity generated from the wind park is also calculated in this project. Later on, the work is devoted to finding the static and dynamic line ratings for “VL3” overhead conductor by using IEEE-738-2006 standard. Furthermore, the project also deals with finding the line current and making its comparison with maximum capacity of overhead conductor (VL3) for loading it in such a way that no any violation of safe ground clearance requirements is observed at all. Besides, the line current, knowing the conductor temperature when it transmits the required electricity in the presence of wind power generation is also an important factor to be taken into consideration. Therefore, based on real-time ambient conditions with actual line loading and with the help of IEEE-738-2006 standard, the conductor temperature is also calculated in this project. At the end, an economic analysis is performed to evaluate the financial advantages related to applying the dynamic line ratings approach in place of traditional static line ratings technique across an overhead conductor (VL3) and to know how much beneficial it is to temporarily postpone the rebuilding and/or construction of a new transmission line. Furthermore, an economic analysis related to wind power system is taken into consideration as well to get familiar with the costs related to building and connecting a 60 MW wind farm with the regional grid.

KIRCHHOFF FORMULA WITH LINE INTEGRAL FORM AND ITS APPLICATIONS

In this paper, Kirchhoff formula has been transformed from surface integral form into a line integral form. The new form of the formula can be applied to separate geometrical optical field from diffraction field, and reduce the time of numerical computation greatly. Based on the new form, an analytical formula of diffraction field in the far zone has been presented for the polygonal aperture illuminated by a uniform plane wave.

分组密码算法uBlock积分攻击的改进

积分攻击是由Daemen等人(doi:10.1007/BFb0052343)于1997年提出的一种密码分析方法,是继差分分析和线性分析之后最有效的密码分析方法之一。作为2018年全国密码算法设计竞赛分组算法的获胜算法,uB-lock抵抗积分攻击的能力受到较多的关注。为了重新评估uBlock家族密码算法抵抗积分攻击的安全性,该文利用单项式传播技术,结合混合整数线性规划(MILP)工具搜索积分区分器,并利用部分和技术进行密钥恢复攻击。对于uBlock-128/128和uBlock-128/256,基于搜索到的9轮积分区分器分别进行了首个11轮和12轮攻击,数据复杂度为2~(127)选择明文,时间复杂度分别为2~(127.06)和2~(224)次加密,存储复杂度分别为2~(44.58)和2~(138)字节;对于uBlock-256/256,基于搜索到的10轮积分区分器进行了首个12轮攻击,数据复杂度为2~(253)选择明文,时间复杂度为2~(253.06)次加密,存储复杂度为2~(44.46)字节。与之前uBlock的最优积分攻击结果相比,uBlock-128/128和uBlock-256/256的攻击轮数分别提高2轮,uBlock-128/256的攻击轮数提高3轮。本文的攻击说明,uBlock针对积分攻击依然有足够的安全冗余。

Linkage analysis and integrated software GAPL for pure-line populations derived from four-way and eight-way crosses

Pure lines derived from multiple parents provide abundant variation for genetic study.However,efficient genetic analysis methods and user-friendly software are still lacking.In this study, we developed linkage analysis methods and integrated analysis software for pure-line populations derived from four-way and eight-way crosses.First, polymorphic markers are classified into different categories according to the number of identifiable alleles in the inbred parents.Expected genotypic probability is then derived for each pair of complete markers, and based on them a maximum likelihood estimate(MLE) of recombination frequency is calculated.An EM algorithm is proposed for calculating recombination frequencies in scenarios that at least one marker is incomplete.A linkage map can thus be constructed using estimated recombination frequencies.We describe a software package called GAPL for recombination frequency estimation and linkage map construction in multi-parental pure-line populations.Both simulation studies and results from a reported four-way cross recombinant inbred line population demonstrate that the proposed method and software can build more accurate linkage maps in shorter times than other published software packages.The GAPL software is freely available from www.isbreeding.net and can also be used for QTL mapping in multi-parental populations.

Analytical solution based on the wavenumber integration method for the acoustic field in a Pekeris waveguide

An exact solution based on the wavenumber integration method is proposed and implemented in a numerical model for the acoustic field in a Pekeris waveguide excited by either a point source in cylindrical geometry or a line source in plane geometry. Besides, an unconditionally stable numerical solution is also presented, which entirely resolves the stability problem in previous methods. Generally the branch line integral contributes to the total field only at short ranges, and hence is usually ignored in traditional normal mode models. However, for the special case where a mode lies near the branch cut, the branch line integral can contribute to the total field significantly at all ranges. The wavenumber integration method is well-suited for such problems. Numerical results are also provided, which show that the present model can serve as a benchmark for sound propagation in a Pekeris waveguide.

ON PARAMETRIC MARCINKIEWICZ INTEGRALS RELATED TO BLOCK SPACES

In this paper,the L2-boundedness of a class of parametric Marcinkiewicz integral μρ Ω,h with kernel function Ω in B 0,0 q(S n-1) for some q>1,and the radial function h(x)∈l∞(Ls)(R +) for 1<s≤∞ are given.The Lp(Rn)(2≤p<∞) boundedness of μ *,ρ Ω,h,λ and μ ρ Ω,h,S with Ω in B 0,0 q(S n-1) and h(|x|)∈l∞(Ls)(R +) in application are obtained.Here μ *,ρ Ω,h,λ and μ ρ Ω,h,S are parametric Marcinkiewicz integrals corresponding to the Littlewood-Paley g* λ function and the Lusin area function S,respectively.

APPLYING FAST WALSH TRANSFORM TECHNIQUE TO ANALYZE HIGH-SPEED DIGITAL INTEGRAL TRANSMISSION LINES

A new approach, based on the waveform relaxation technique and fast Walsh trans-form, is presented to analyze the coupled loosy transmission lines (CLTL) with arbitrary terminalnetworks. The simulation accuracy of the new method can be greatly improved, the disadvantagewhich always exists in previous methods can be avoided and a considerable saving in time andmemory of CPU is obtained.

An Integration Depending on Parameter Ф for Analytical Solution of the Compression of Thin Workpiece

An integration depending on the parameter Ф has been applied to the function Фdx so that an explicit formula of specific pressure calculation and an analytical solution are obtained.It has been found that the analytical solution is basically the same as the prevailing numerical one which is from the formula There- fore,it is justified theoretically that for the slip line field in the compression of a thin workpiece a parametric integra- tion is perfectly possible for satisfactory analytical solution.

Innovative integrating of hot-dipped(aluminizing) galvanizing lines and engineering of related equipment

Based on the continuous hot(aluminizing) galvanizing lines and related non-standard equipment of Meishan Steel,this paper presents the main contents of such integrated engineering and designing of equipment for the two hot-dipped galvanizing lines,as well as innovative techniques and their features.Led by Cold Rolling Project Department of Meishan Steel,Baosteel Engineering and Technology Group Co.,Ltd. ,working as the responsible unit of this project,develops key technologies and integrates the system by combining the equipment design and development capacity of Baosteel Engineering,research and development capacity of Research Institute of Baosteel,process production and equipment maintenance experience of Cold Rolling Plant,Equipment Department and Manufacture Department of Baosteel Corporation.Core technologies of the two lines are developed and designed through original and integrated innovation and innovation after digestion,focusing on eight core techniques in process equipment,annealing furnace,fluid installation and EIC system.As a result of integration in key process equipment,industrial furnace and EIC control,the cold rolling hot dipped(aluminizing) galvanizing lines take on a new look and reach an annual capacity of 200 000 t galvanized products and 250 000 t galvanized aluminium base strips respectively.These two world advanced lines are able to produce hot-dipped galvanizing products with high quality surface and fine shape,such as household appliance board and senior construction board.As a result, the process and equipment technology of the lines have formed domestic intellectual property.Thus,the technology has reference value for self-integration and equipment technology innovation of similar lines.

INTEGRAL REPRESENTATION OF SLOWLY GROWING EQUIDISTANT SPLINES

In this paper we consider polynomial splines S(x) with equidistant nodes which may grow a5 O (|x|~5). We present an integral representation of such splines with a distribution kernel. This repre- sentation is related to the Fourier integral of slowly growing functions. The part of the Fourier ex- ponentials herewith play the so called exponential splines by Schoenberg. The integral representation provides a flexible tool for dealing with the growing equidistant splines. First. it allows us to con- struct a rich library of splines possessing the property that translations of any such spline form a ba- sis of corresponding spline space. It is shown that any such spline is associated with a dual spline whose translations form a biorthogonal basis. As examples we present solutions of the problems of projection of a growing function onto spline spaces and of spline interpolation of growing func- tion. We derive formulas for approximate evaluation of splines projecting a function onto the spline space and establish therewith exact estimations of the approximation errors.

Integrated On-line Instrumentation System of Magnetic Properties Measurement

A portable microcomputer-controlled inspection system has been developed for detection of magnetic properties of soft magnetic materials. It incorporates custom designed software for control of the magnetic field during operation such as demagnetization, field sweeping, and for data logging and analysis. Results are recorded using a 12-bit analog to digital converter and are then stored on disk. The magnetic hysteresis loop and Barkhausen noise data can be converted into important magnetic parameters: coecivity, remanence, and hysteresis loss, Barkhausen amplitude, and Barkhausen noise energy. This system incorporated with the magnetostriction, and magnetoacoustic emission, is then related with the nondestructive detection of material degradation.

Pattern of Opening Up,Integration and Reshaping Economic Geography——A New Economic Geography Analysis of the Belt and Road Initiative and the Yangtze River Economic Belt

Since the 18 th National Congress of the Communist Party of China,the central government has implemented several regional development plans,including the Belt and Road Initiative and the Yangtze River Economic Belt,aiming to reshape the economic geography of China.This paper is based on the Belt and Road Initiative and the Yangtze River Economic Belt and outlines the pattern of opening up.To analyze the impact of the pattern of opening up on the regional integration of China's economic geography,this paper has developed a new economic geography model comprised of four regions and two countries.From this,it draws some conclusions.First,the change of pattern of opening up will lead to shifting centripetal and centrifugal forces from the international market.The greater the scale of neighboring markets,the stronger the agglomeration force will be; the more product varieties made in neighboring countries,the more potent its dispersion force.Second,developing an inland international trade corridor will decentralize industry distribution and reduce the impact of external market integration on home countries.Third,the inland international trade corridor could alter the effect of domestic market integration on the economic geography.When domestic integration occurs prior to international integration,further integration of a domestic market leads to greater industrial distribution along a bell-shaped curve,which describes economic activities from dispersion to agglomeration to decentralization.Therefore,developing an integrated market system of major countries and opening a corridor between China and Europe is not only the key to reshaping China's economic geography but also the path to solving the problems of the Heihe-Tengchong Line.

The Integrated System of Aerial Photogrammetry and Tower Locations Optimization for Transmission Lines

This paper describes the functions and the features of the integrated system of aerial survey and tower locations optimization for transmission lines, which includes all stages from data acquisition, data transmission and data processing to automatic optimization of the tower locations and drawing. The paper also briefly describes the economic benefit gained from this system, and finally proposes the directions of the future development for this system.

Independent integration practice on revamping 4200/3500 mm medium and heavy plate rolling mill of Bayi Steel in terms of process and make-up equipment

This study systematically reviews and summarizes relocation and revamping project of medium and heavy plate rolling mill in Bayi Steel.After detailed analysis was made on the main deficiency and problems of the original production line of Pudong Steel,systematical integration and improvement for the process and equipment were carried out accordingly.Through innovative practice of integration,Baosteel Engineering has greatly improved the technical process and equipment make-up,as well as the grades and quality of the products.The original man-operating line is redesigned into an automatic system controlled by Level 2 computer,reaching the advanced technical level of home-made medium and heavy plate production line in recent years.The project is constructed in two phases;The original product mix of Pudong Steel is maintained as much as possible in Phase I.After 3 500 mm single stand finishing mill is put into operation, capacity of hot- rolling medium and heavy plate reaches 650 000 t/a,with the maximum thickness at 40 mm.Equipment or facilities are reserved for production of pipeline steel and other grades.Pipeline steel, vessel plate,wind power plate and other TMCP and heat treatment products are projected in PhaseⅡ.After 4 200 mm roughing mill and its matched facilities are put into operation,capacity of hot-rolling medium and heavy plate will reach 120 000 t/a,with the maximum thickness at 80 mm.Through the optimization of process equipment and its function,the relocated medium and heavy plate mill production line has reached advanced level among similar domestic mills.The prominent achievements of the project are the revamping of thickness control system of the finishing mill and the newly installed shape control system.Automation control of the whole line is realized.