The functional and structural integrity of the blood-brain barrier is crucial in maintaining homeostasis in the brain microenvironment;however,the molecular mechanisms underlying the formation and function of the bloo...The functional and structural integrity of the blood-brain barrier is crucial in maintaining homeostasis in the brain microenvironment;however,the molecular mechanisms underlying the formation and function of the blood-brain barrier remain poorly understood.The major facilitator superfamily domain containing 2A has been identified as a key regulator of blood-brain barrier function.It plays a critical role in promoting and maintaining the formation and functional stability of the blood-brain barrier,in addition to the transport of lipids,such as docosahexaenoic acid,across the blood-brain barrier.Furthermore,an increasing number of studies have suggested that major facilitator superfamily domain containing 2A is involved in the molecular mechanisms of blood-brain barrier dysfunction in a variety of neurological diseases;however,little is known regarding the mechanisms by which major facilitator superfamily domain containing 2A affects the blood-brain barrier.This paper provides a comprehensive and systematic review of the close relationship between major facilitator superfamily domain containing 2A proteins and the blood-brain barrier,including their basic structures and functions,cross-linking between major facilitator superfamily domain containing 2A and the blood-brain barrier,and the in-depth studies on lipid transport and the regulation of blood-brain barrier permeability.This comprehensive systematic review contributes to an in-depth understanding of the important role of major facilitator superfamily domain containing 2A proteins in maintaining the structure and function of the blood-brain barrier and the research progress to date.This will not only help to elucidate the pathogenesis of neurological diseases,improve the accuracy of laboratory diagnosis,and optimize clinical treatment strategies,but it may also play an important role in prognostic monitoring.In addition,the effects of major facilitator superfamily domain containing 2A on blood-brain barrier leakage in various diseases and the research progress on cross-blood-brain barrier drug delivery are summarized.This review may contribute to the development of new approaches for the treatment of neurological diseases.展开更多
BACKGROUND The 5-year survival rate of patients with colorectal cancer(CRC)in China is only 56.9%,highlighting the need for new therapeutic drugs.Previous studies have shown that matrine exhibits antitumor effects by ...BACKGROUND The 5-year survival rate of patients with colorectal cancer(CRC)in China is only 56.9%,highlighting the need for new therapeutic drugs.Previous studies have shown that matrine exhibits antitumor effects by inducing apoptosis.However,the mechanism by which matrine regulates antiapoptotic proteins in CRC remains unclear.AIM To identify apoptotic proteins from proteomics and investigate the role of matrine in impeding CRC apoptosis by regulating these proteins.METHODS Tumor and adjacent normal tissues were collected from 52 patients with CRC who underwent surgery between January and December 2021.Data-independent acquisition quantitative proteomic analysis was performed to identify differentially expressed apoptotic proteins.The selected apoptotic proteins were identified through their association with tumor-node-metastasis(TNM)stage and prognosis,then confirmed by immunohistochemical(IHC)staining in validation cohort.In vitro,the role of matrine or apoptotic proteins on cancer cells were analyzed.RESULTS Compared to normal tissues,88 anti-apoptotic proteins from proteomic results were selected.Among them,Shankassociated RH domain interactor(SHARPIN)was identified because of its relationship with TNM stage and overall survival in TCGA database.In the IHC-confirmed cohort,SHARPIN was highly expressed in CRC tissues and localized in the cytoplasm.Higher SHARPIN expression was associated with TNM stage,carbohydrate antigen 153 levels,and gross type compared to low expression.SHARPIN knockdown promoted apoptosis,significantly upregulated the expression of Bcl-2 associated agonist of cell death,Bcl-2 associated X protein,caspase 3,and caspase 8,and downregulated B-cell lymphoma-2(P<0.05).Importantly,matrine treatment promoted apoptosis and reversed the proliferation,invasion,and migration of CRC cells by repressing SHARPIN.CONCLUSION SHARPIN was identified as an upregulated anti-apoptotic protein in CRC,and matrine exhibited anticancer effects by downregulating its expression.Thus,matrine appears to be a promising drug for CRC.展开更多
The mitogen-activated protein kinase kinase kinase kinases(MAP4Ks)signaling pathway plays a pivotal role in axonal regrowth and neuronal degeneration following insults.Whether targeting this pathway is beneficial to b...The mitogen-activated protein kinase kinase kinase kinases(MAP4Ks)signaling pathway plays a pivotal role in axonal regrowth and neuronal degeneration following insults.Whether targeting this pathway is beneficial to brain injury remains unclear.In this study,we showed that adeno-associated virus-delivery of the Citron homology domain of MAP4Ks effectively reduces traumatic brain injury-induced reactive gliosis,tauopathy,lesion size,and behavioral deficits.Pharmacological inhibition of MAP4Ks replicated the ameliorative effects observed with expression of the Citron homology domain.Mechanistically,the Citron homology domain acted as a dominant-negative mutant,impeding MAP4K-mediated phosphorylation of the dishevelled proteins and thereby controlling the Wnt/β-catenin pathway.These findings implicate a therapeutic potential of targeting MAP4Ks to alleviate the detrimental effects of traumatic brain injury.展开更多
Based on the microscopic phase-field model, ordered domain interfaces formed between D022 (Ni3V) phases along [001] direction in Ni75AlxV25-x alloys were simulated, and the effects of atomic structure on the migrati...Based on the microscopic phase-field model, ordered domain interfaces formed between D022 (Ni3V) phases along [001] direction in Ni75AlxV25-x alloys were simulated, and the effects of atomic structure on the migration characteristic and solute segregation of interfaces were studied. It is found that the migration ability is related to the atomic structure of interfaces, and three kinds of interfaces can migrate except the interface (001)//(002) which has the characteristic of L12 (Ni3Al) structure. V atoms jump to the nearest neighbor site and substitute for Ni, and vice versa. Because of the site selectivity behaviors of jumping atoms, the number of jumping atoms during the migration is the least and the jumping distance of atoms is the shortest among all possible modes, and the atomic structures of interfaces are unchanged before and after the migration. The preferences and degree of segregation or depletion of alloy elements are also related to the atomic structure of interface.展开更多
Wavefield separation of multicomponent seismic data to image subsurface structures can be realized in either the space domain or the wavenumber domain. However, as the particle velocity components used in the wavenumb...Wavefield separation of multicomponent seismic data to image subsurface structures can be realized in either the space domain or the wavenumber domain. However, as the particle velocity components used in the wavenumber-domain wavefield separation are not defined at the same grid point with the staggered-grid finite-difference method for elastic wavefield simulation, we propose the wavenumber-domain interpolation method to estimate the required values at the common grid points prior to the wavenumber-domain true-amplitude wavefield separation. Moreover, numerical experiments show that the wavenumber-domain interpolation method has high interpolation accuracy and the trueamplitude wavefield separation method shows good amplitude preservation. The application of the proposed methodology to elastic reverse-time migration can obtain good amplitudepreserved images even in the case of some velocity error.展开更多
Two DNA fragments encoding PDZ domain (21-110 residues) and BAR domain ( 150-360 residues) from PICK1 (1-416 residues) were amplified by PCR and then introduced into vectors, pET-32M and pMAL-e2X respectively to...Two DNA fragments encoding PDZ domain (21-110 residues) and BAR domain ( 150-360 residues) from PICK1 (1-416 residues) were amplified by PCR and then introduced into vectors, pET-32M and pMAL-e2X respectively to generate recombinant plasmids, pE-pdz and pM-bar. Having been separately transferred into the hosts E. coli BL21 and E. coli JM109, these two strains can express fusion proteins: His-tagged PDZ(PDZ domain) and maltose binding protein-BAR( MBP-BAR domain) respectively, as confirmed by both SDS-PAGE and Wostem blotting. The interaction between these two domains is dose-dependence, as identified by a pull-down test. Moreover, it has been shown from the ELISA analysis that the actual amount of PDZ bound to MBP-BAR-amylose beads reaches ( 16 ± 0. 5)%, as calculated by the molar ratio of PDZ to MBP-BAR. In addition, the interaction between BAR(bait) and PDZ(prey) in vivo was also examined with a yeast two-hybrid system.展开更多
The normalized weighted capacity reliability index of a sub-domain interconnection large scale communication network is defined and a new algorithm to calculate the reliability index is proposed, The proposed algorith...The normalized weighted capacity reliability index of a sub-domain interconnection large scale communication network is defined and a new algorithm to calculate the reliability index is proposed, The proposed algorithm can be performed using logical or algebraic operation by means of computer-aided programming and the correctness of each key step is validated in detail. This paper takes the sub-domain interconnection symmetrical topologi- cal network for a typical example to calculate the network reliability index and verifies the correctness of the proposed algorithm us- ing the real measured statistical data. The real measured results are well in accordance with the results obtained by the proposed algorithm. The result shows that the proposed algorithm is a valid means to estimate the reliability index of a sub-domain intercon- nection large-scale communication network.展开更多
In order to indicate the performances of a large-scale communication network with domain partition and interconnection today, a kind of reliability index weighed by normalized capacity is defined. Based on the route r...In order to indicate the performances of a large-scale communication network with domain partition and interconnection today, a kind of reliability index weighed by normalized capacity is defined. Based on the route rules of network with domain partition and interconnection, the interconnection indexes among the nodes within the domain and among the domains are given from several aspects. It is expatiated on that the index can thoroughly represent the effect on the reliability index of the objective factor and the subjective measures of the designer, which obeys the route rules of a network with domain partition and interconnection. It is discussed that the defined index is rational and compatible with the traditional index.展开更多
The typical magnetic domains of Sm(CObalFe0.25Cuo.07Zr0.02)7.4 magnets quenched through various heattreatment steps have been revealed by using magnetic force microscopy (MFM). For the specimens in which the nomin...The typical magnetic domains of Sm(CObalFe0.25Cuo.07Zr0.02)7.4 magnets quenched through various heattreatment steps have been revealed by using magnetic force microscopy (MFM). For the specimens in which the nominal c-axis is perpendicular to the imaging plane, the domain configurations change from plate-like for the as-sintered magnet to corrugation and spike-like for the homogenized one, and then to a coarse and finally to a finer domain structure when isothermally aged at 830℃ and then annealed at 400℃. However, only plate-like domains can be detected on the surfaces with the nominal c-axis parallel to the imaging plane. The finer domain (so-called interaction domain) is a characteristic magnetic domain pattern of the SmCo 2:IT-type magnets with high coercivities. Domain walls in a zigzag shape are revealed by means of MFM in final bulk SraCo 2:17-type sintered magnets.展开更多
Consideration of structure-foundation-soil dynamic interaction is a basic requirement in the evaluation of the seismic safety of nuclear power facilities. An efficient and accurate dynamic interaction numerical model ...Consideration of structure-foundation-soil dynamic interaction is a basic requirement in the evaluation of the seismic safety of nuclear power facilities. An efficient and accurate dynamic interaction numerical model in the time domain has become an important topic of current research. In this study, the scaled boundary finite element method (SBFEM) is improved for use as an effective numerical approach with good application prospects. This method has several advantages, including dimensionality reduction, accuracy of the radial analytical solution, and unlike other boundary element methods, it does not require a fundamental solution. This study focuses on establishing a high performance scaled boundary finite element interaction analysis model in the time domain based on the acceleration unit-impulse response matrix, in which several new solution techniques, such as a dimensionless method to solve the interaction force, are applied to improve the numerical stability of the actual soil parameters and reduce the amount of calculation. Finally, the feasibility of the time domain methods are illustrated by the response of the nuclear power structure and the accuracy of the algorithms are dynamically verified by comparison with the refinement of a large-scale viscoelastic soil model.展开更多
Studies on nonlinear behavior at oil/water interface membrane were performed. This system showed rhythmic oscillations and chaos of electrical potential in a given concentration domain. The nonlinear behavior response...Studies on nonlinear behavior at oil/water interface membrane were performed. This system showed rhythmic oscillations and chaos of electrical potential in a given concentration domain. The nonlinear behavior response at the liquid membrane apparently resembled that of biological chemoreceptive membrane. The possibility of developing a new type of chemical sensor with the ability to simulate substance equilibrium in living organisms was suggested in the paper.展开更多
Amplitude variations with offset or incident angle (AVO/AVA) inversion are typically combined with statistical methods, such as Bayesian inference or deterministic inversion. We propose a joint elastic inversion met...Amplitude variations with offset or incident angle (AVO/AVA) inversion are typically combined with statistical methods, such as Bayesian inference or deterministic inversion. We propose a joint elastic inversion method in the time and frequency domain based on Bayesian inversion theory to improve the resolution of the estimated P- and S-wave velocities and density. We initially construct the objective function using Bayesian inference by combining seismic data in the time and frequency domain. We use Cauchy and Gaussian probability distribution density functions to obtain the prior information for the model parameters and the likelihood function, respectively. We estimate the elastic parameters by solving the initial objective function with added model constraints to improve the inversion robustness. The results of the synthetic data suggest that the frequency spectra of the estimated parameters are wider than those obtained with conventional elastic inversion in the time domain. In addition, the proposed inversion approach offers stronger antinoising compared to the inversion approach in the frequency domain. Furthermore, results from synthetic examples with added Gaussian noise demonstrate the robustness of the proposed approach. From the real data, we infer that more model parameter details can be reproduced with the proposed joint elastic inversion.展开更多
AIM: To investigate the role of P-selectin, intercellular adhesion molecule-1 (ICAM-1) and dendritic cells (DCs)in liver/kidney of rats with hepatic/renal ischemiareperfusion injury and the preventive effect of anti-P...AIM: To investigate the role of P-selectin, intercellular adhesion molecule-1 (ICAM-1) and dendritic cells (DCs)in liver/kidney of rats with hepatic/renal ischemiareperfusion injury and the preventive effect of anti-Pselectin lectin-EGF domain monoclonal antibody (anti-PsLEGFmAb) on the injury.METHODS: Rat models of hepatic and renal ischemiareperfusion were established. The rats were then divided into two groups, one group treated with anti-PsL-EGFmAb(n = 20) and control treated with saline (n = 20). Both groups were subdivided into four groups according to reperfusion time (1, 3, 6 and 24 h). The sham-operated group (n = 5) served as a control group. DCs were observed by the microscopic image method, while P-selectin and ICAM-1 were analyzed by immunohistochemistry.RESULTS: P-selectin increased significantly in hepatic sinusoidal endothelial cells and renal tubular epithelial cells 1 h after ischemia-reperfusion, and the expression of ICAM-1 was up-regulated in hepatic sinusoid and renal vessels after 6 h. CD1a+CD80+DCs gradually increased in hepatic sinusoidal endothelium and renal tubules and interstitium 1 h after ischemia-reperfusion, and there was the most number of DCs in 24-h group. The localization of DCs was associated with rat hepatic/renal function.These changes became less significant in rats treated with anti-PsL-EGFmAb.CONCLUSION: DCs play an important role in immune pathogenesis of hepatic/renal ischemia-reperfusion injury.Anti-PsL-EGFmAb may regulate and inhibit local DC immigration and accumulation in liver/kidney.展开更多
Ferromagnetic shape memory Ni-Mn-Ga films with 7M modulated structure were prepared on MgO (001) substrates by magnetron sputtering. Magnetization process with a typical two-hysteresis loop indicates the occurrence ...Ferromagnetic shape memory Ni-Mn-Ga films with 7M modulated structure were prepared on MgO (001) substrates by magnetron sputtering. Magnetization process with a typical two-hysteresis loop indicates the occurrence of the reversible magnetic field-induced reorientation. Magnetic domain structure and twin structure of the film were controlled by the in- terplay of the magnetic and temperature field. With cooling under an out-of-plane magnetic field, the evolution of magnetic domain structure reveals that martensitic transformation could be divided into two periods: nucleation and growth. With an in-plane magnetic field applied to a thermomagnetic-treated film, the evolution of magnetic domain structure gives evidence of a reorientation of twin variants of martensite. A microstructural model is described to define the twin structure and to produce the magnetic domain structure at the beginning of martensitic transformation; based on this model, the relationship between the twin structure and the magnetic domain structure for the treated film under an in-plane field is also described.展开更多
Plant cellulose synthases (CesAs) are the key enzymes necessary for cellulose biosynthesis. In Arabidopsis, two distinct groups of three CesAs each are necessary for cellulose synthesis during primary and secondary ce...Plant cellulose synthases (CesAs) are the key enzymes necessary for cellulose biosynthesis. In Arabidopsis, two distinct groups of three CesAs each are necessary for cellulose synthesis during primary and secondary cell wall formation. It has also been suggested that such three CesAs interact with each other to form plasma-membrane bound rosette complexes that are functional during cellulose production. However, in vivo demonstration of such assemblies of three CesAs into rosettes has not been possible. We used yeast two-hybrid assays to demonstrate the possible interactions among several CesAs from Arabidopsis and aspen via their N-terminal zinc-binding domains (ZnBDs). While strong positive interactions were detected among ZnBDs from secondary wall associated CesAs of both Arabidopsis and aspen, the intergeneric interactions between Arabidopsis and aspen CesAs were weak. Moreover, in aspen, three primary wall associated CesA ZnBDs positively interacted with each other as well as with secondary CesAs. These results suggest that ZnBDs from either primary or secondary CesAs, and even from different plant species could interact but are perhaps insufficient for specificities of such interactions among CesAs. These observations suggest that some other more specific interacting regions might exist within CesAs. It is also possible that some hitherto unknown mechanism exists in plants for assembling the rosette complexes with different compositions of CesAs. Understanding how cellulose is synthesized will have a direct impact on utilization of lignocellulosic biomass for bioenergy production.展开更多
A modified time domain interpolation method is proposed for orthogonal frequency division multiplexing(OFDM)systems to address the problem that time domain interpolation in the least square(LS)channel estimation metho...A modified time domain interpolation method is proposed for orthogonal frequency division multiplexing(OFDM)systems to address the problem that time domain interpolation in the least square(LS)channel estimation method based on comb-type pilots cannot choose the pilot spacing flexibly.Firstly,the estimated channel frequency response(CFR)at pilot positions in the frequency domain is obtained by LS channel estimation based on comb-type pilots,and the estimated channel impulse response(CIR)in the time domain is obtained by linear interpolation and inverse fast Fourier transform(IFFT).Secondly,the error of the estimated CIR obtained by linear interpolation is analyzed by theoretical deduction,and a method for correcting it is proposed.Finally,an estimated CFR at all subcarrier positions in the frequency domain is obtained by performing zero padding in the time domain and fast Fourier transform(FFT)on the modified CIR.The simulation results suggest that the proposed method gives similar performance to time domain interpolation,yet it does not need to meet the condition of time domain interpolation that the number of subcarriers must be an integral multiple of pilot spacing to use it.The proposed method allows for flexible pilot spacing,reducing the number of pilots and the consumption of subcarriers used for channel estimation.展开更多
Due to the strong electromagnetic interferences and human interference,traditional electromagnetic methods cannot obtain high quality resistivity data of mineral deposits in Chinese mines.The wide field electromagneti...Due to the strong electromagnetic interferences and human interference,traditional electromagnetic methods cannot obtain high quality resistivity data of mineral deposits in Chinese mines.The wide field electromagnetic method(WFEM),in which the pseudo-random signal is taken as the transmitter source,can extract high quality resistivity data in areas with sever interference by only measuring the electric field component.We use the WFEM to extract the resistivity information of the Dongguashan mine in southeast China.Compared with the audio magnetotelluric(AMT)method,and the controlled source audio-frequency magnetotelluric(CSAMT) method,the WFEM can obtain data with higher quality and simpler operations.The inversion results indicate that the WFEM can accurately identify the location of the main ore-body,which can be used for deep mine exploration in areas with strong interference.展开更多
In this paper we investigate simultaneous approximation for arbitrary system of nodes on smooth domain in complex plane. Some results which are better than those of known theorems are obtained.
In this paper, a 3D time domain technique is adopted to calculate the coupled hydrodynamic interaction between two bodies without flare in waves. For verifying the code, two same cylinders are selected to calculate co...In this paper, a 3D time domain technique is adopted to calculate the coupled hydrodynamic interaction between two bodies without flare in waves. For verifying the code, two same cylinders are selected to calculate coupled hydrodynamic effects by comparison with the results obtained by 3D frequency method which has been proved to be efficient for solving such problems. In order to improve efficiency of calculation, the effect of history time has been discussed, and an improved method is presented. Moreover, the effect of lateral separation distance is also discussed in detail. The technique developed here may serve as a more rigorous tool to analyze the related transient problems of two ships doing underway replenishment in waves.展开更多
The distributed Lagrange multiplier/fictitious domain(DLM/FD)-mixed finite element method is developed and analyzed in this paper for a transient Stokes interface problem with jump coefficients.The semi-and fully disc...The distributed Lagrange multiplier/fictitious domain(DLM/FD)-mixed finite element method is developed and analyzed in this paper for a transient Stokes interface problem with jump coefficients.The semi-and fully discrete DLM/FD-mixed finite element scheme are developed for the first time for this problem with a moving interface,where the arbitrary Lagrangian-Eulerian(ALE)technique is employed to deal with the moving and immersed subdomain.Stability and optimal convergence properties are obtained for both schemes.Numerical experiments are carried out for different scenarios of jump coefficients,and all theoretical results are validated.展开更多
基金supported by the National Natural Science Foundation of China,No.82104412(to TD)Shaanxi Provincial Key R&D Program,No.2023-YBSF-165(to TD)+1 种基金the Natural Science Foundation of Shaanxi Department of Science and Technology,No.2018JM7022(to FM)Shaanxi Provincial Key Industry Chain Project,No.2021ZDLSF04-11(to PW)。
文摘The functional and structural integrity of the blood-brain barrier is crucial in maintaining homeostasis in the brain microenvironment;however,the molecular mechanisms underlying the formation and function of the blood-brain barrier remain poorly understood.The major facilitator superfamily domain containing 2A has been identified as a key regulator of blood-brain barrier function.It plays a critical role in promoting and maintaining the formation and functional stability of the blood-brain barrier,in addition to the transport of lipids,such as docosahexaenoic acid,across the blood-brain barrier.Furthermore,an increasing number of studies have suggested that major facilitator superfamily domain containing 2A is involved in the molecular mechanisms of blood-brain barrier dysfunction in a variety of neurological diseases;however,little is known regarding the mechanisms by which major facilitator superfamily domain containing 2A affects the blood-brain barrier.This paper provides a comprehensive and systematic review of the close relationship between major facilitator superfamily domain containing 2A proteins and the blood-brain barrier,including their basic structures and functions,cross-linking between major facilitator superfamily domain containing 2A and the blood-brain barrier,and the in-depth studies on lipid transport and the regulation of blood-brain barrier permeability.This comprehensive systematic review contributes to an in-depth understanding of the important role of major facilitator superfamily domain containing 2A proteins in maintaining the structure and function of the blood-brain barrier and the research progress to date.This will not only help to elucidate the pathogenesis of neurological diseases,improve the accuracy of laboratory diagnosis,and optimize clinical treatment strategies,but it may also play an important role in prognostic monitoring.In addition,the effects of major facilitator superfamily domain containing 2A on blood-brain barrier leakage in various diseases and the research progress on cross-blood-brain barrier drug delivery are summarized.This review may contribute to the development of new approaches for the treatment of neurological diseases.
基金Supported by National Key Development Plan for Precision Medicine Research,No.2017YFC0910002.
文摘BACKGROUND The 5-year survival rate of patients with colorectal cancer(CRC)in China is only 56.9%,highlighting the need for new therapeutic drugs.Previous studies have shown that matrine exhibits antitumor effects by inducing apoptosis.However,the mechanism by which matrine regulates antiapoptotic proteins in CRC remains unclear.AIM To identify apoptotic proteins from proteomics and investigate the role of matrine in impeding CRC apoptosis by regulating these proteins.METHODS Tumor and adjacent normal tissues were collected from 52 patients with CRC who underwent surgery between January and December 2021.Data-independent acquisition quantitative proteomic analysis was performed to identify differentially expressed apoptotic proteins.The selected apoptotic proteins were identified through their association with tumor-node-metastasis(TNM)stage and prognosis,then confirmed by immunohistochemical(IHC)staining in validation cohort.In vitro,the role of matrine or apoptotic proteins on cancer cells were analyzed.RESULTS Compared to normal tissues,88 anti-apoptotic proteins from proteomic results were selected.Among them,Shankassociated RH domain interactor(SHARPIN)was identified because of its relationship with TNM stage and overall survival in TCGA database.In the IHC-confirmed cohort,SHARPIN was highly expressed in CRC tissues and localized in the cytoplasm.Higher SHARPIN expression was associated with TNM stage,carbohydrate antigen 153 levels,and gross type compared to low expression.SHARPIN knockdown promoted apoptosis,significantly upregulated the expression of Bcl-2 associated agonist of cell death,Bcl-2 associated X protein,caspase 3,and caspase 8,and downregulated B-cell lymphoma-2(P<0.05).Importantly,matrine treatment promoted apoptosis and reversed the proliferation,invasion,and migration of CRC cells by repressing SHARPIN.CONCLUSION SHARPIN was identified as an upregulated anti-apoptotic protein in CRC,and matrine exhibited anticancer effects by downregulating its expression.Thus,matrine appears to be a promising drug for CRC.
基金supported by the TARCC,Welch Foundation Award(I-1724)the Decherd Foundationthe Pape Adams Foundation,NIH grants NS092616,NS127375,NS117065,NS111776。
文摘The mitogen-activated protein kinase kinase kinase kinases(MAP4Ks)signaling pathway plays a pivotal role in axonal regrowth and neuronal degeneration following insults.Whether targeting this pathway is beneficial to brain injury remains unclear.In this study,we showed that adeno-associated virus-delivery of the Citron homology domain of MAP4Ks effectively reduces traumatic brain injury-induced reactive gliosis,tauopathy,lesion size,and behavioral deficits.Pharmacological inhibition of MAP4Ks replicated the ameliorative effects observed with expression of the Citron homology domain.Mechanistically,the Citron homology domain acted as a dominant-negative mutant,impeding MAP4K-mediated phosphorylation of the dishevelled proteins and thereby controlling the Wnt/β-catenin pathway.These findings implicate a therapeutic potential of targeting MAP4Ks to alleviate the detrimental effects of traumatic brain injury.
基金Projects (50941020, 10902086, 50875217, 20903075) supported by the National Natural Science Foundation of ChinaProjects (SJ08-ZT05, SJ08-B14) supported by the Natural Science Foundation of Shaanxi Province, ChinaProject (CX200905) supported by the Doctorate Foundation of Northwestern Polytechnical University, China
文摘Based on the microscopic phase-field model, ordered domain interfaces formed between D022 (Ni3V) phases along [001] direction in Ni75AlxV25-x alloys were simulated, and the effects of atomic structure on the migration characteristic and solute segregation of interfaces were studied. It is found that the migration ability is related to the atomic structure of interfaces, and three kinds of interfaces can migrate except the interface (001)//(002) which has the characteristic of L12 (Ni3Al) structure. V atoms jump to the nearest neighbor site and substitute for Ni, and vice versa. Because of the site selectivity behaviors of jumping atoms, the number of jumping atoms during the migration is the least and the jumping distance of atoms is the shortest among all possible modes, and the atomic structures of interfaces are unchanged before and after the migration. The preferences and degree of segregation or depletion of alloy elements are also related to the atomic structure of interface.
基金supported by the National Science Foundation of China(No.41174100)the Large-scale Oil and Gas Field and Coalbed Methane Development Major Projects(No.2011ZX05019-008-08)the China National Petroleum Corporation(No.2014A-3609)
文摘Wavefield separation of multicomponent seismic data to image subsurface structures can be realized in either the space domain or the wavenumber domain. However, as the particle velocity components used in the wavenumber-domain wavefield separation are not defined at the same grid point with the staggered-grid finite-difference method for elastic wavefield simulation, we propose the wavenumber-domain interpolation method to estimate the required values at the common grid points prior to the wavenumber-domain true-amplitude wavefield separation. Moreover, numerical experiments show that the wavenumber-domain interpolation method has high interpolation accuracy and the trueamplitude wavefield separation method shows good amplitude preservation. The application of the proposed methodology to elastic reverse-time migration can obtain good amplitudepreserved images even in the case of some velocity error.
基金the National Natural Science Foundation of China(No 30400065)
文摘Two DNA fragments encoding PDZ domain (21-110 residues) and BAR domain ( 150-360 residues) from PICK1 (1-416 residues) were amplified by PCR and then introduced into vectors, pET-32M and pMAL-e2X respectively to generate recombinant plasmids, pE-pdz and pM-bar. Having been separately transferred into the hosts E. coli BL21 and E. coli JM109, these two strains can express fusion proteins: His-tagged PDZ(PDZ domain) and maltose binding protein-BAR( MBP-BAR domain) respectively, as confirmed by both SDS-PAGE and Wostem blotting. The interaction between these two domains is dose-dependence, as identified by a pull-down test. Moreover, it has been shown from the ELISA analysis that the actual amount of PDZ bound to MBP-BAR-amylose beads reaches ( 16 ± 0. 5)%, as calculated by the molar ratio of PDZ to MBP-BAR. In addition, the interaction between BAR(bait) and PDZ(prey) in vivo was also examined with a yeast two-hybrid system.
文摘The normalized weighted capacity reliability index of a sub-domain interconnection large scale communication network is defined and a new algorithm to calculate the reliability index is proposed, The proposed algorithm can be performed using logical or algebraic operation by means of computer-aided programming and the correctness of each key step is validated in detail. This paper takes the sub-domain interconnection symmetrical topologi- cal network for a typical example to calculate the network reliability index and verifies the correctness of the proposed algorithm us- ing the real measured statistical data. The real measured results are well in accordance with the results obtained by the proposed algorithm. The result shows that the proposed algorithm is a valid means to estimate the reliability index of a sub-domain intercon- nection large-scale communication network.
文摘In order to indicate the performances of a large-scale communication network with domain partition and interconnection today, a kind of reliability index weighed by normalized capacity is defined. Based on the route rules of network with domain partition and interconnection, the interconnection indexes among the nodes within the domain and among the domains are given from several aspects. It is expatiated on that the index can thoroughly represent the effect on the reliability index of the objective factor and the subjective measures of the designer, which obeys the route rules of a network with domain partition and interconnection. It is discussed that the defined index is rational and compatible with the traditional index.
基金Project supported by the Beijing Municipal Science and Technology Committee Key Project, China (Grant No D0406002000091)the National Natural Science Foundation of China (Grant No 10374110)
文摘The typical magnetic domains of Sm(CObalFe0.25Cuo.07Zr0.02)7.4 magnets quenched through various heattreatment steps have been revealed by using magnetic force microscopy (MFM). For the specimens in which the nominal c-axis is perpendicular to the imaging plane, the domain configurations change from plate-like for the as-sintered magnet to corrugation and spike-like for the homogenized one, and then to a coarse and finally to a finer domain structure when isothermally aged at 830℃ and then annealed at 400℃. However, only plate-like domains can be detected on the surfaces with the nominal c-axis parallel to the imaging plane. The finer domain (so-called interaction domain) is a characteristic magnetic domain pattern of the SmCo 2:IT-type magnets with high coercivities. Domain walls in a zigzag shape are revealed by means of MFM in final bulk SraCo 2:17-type sintered magnets.
基金the State Key Program of National Natural Science of China under Grant No.51138001Science Fund for Creative Research Groups of the National Natural Science Foundation of China under Grant No.51121005Open Research Fund Program of State key Laboratory of Hydro science and Engineering under Grant No.shlhse-2010-C-03
文摘Consideration of structure-foundation-soil dynamic interaction is a basic requirement in the evaluation of the seismic safety of nuclear power facilities. An efficient and accurate dynamic interaction numerical model in the time domain has become an important topic of current research. In this study, the scaled boundary finite element method (SBFEM) is improved for use as an effective numerical approach with good application prospects. This method has several advantages, including dimensionality reduction, accuracy of the radial analytical solution, and unlike other boundary element methods, it does not require a fundamental solution. This study focuses on establishing a high performance scaled boundary finite element interaction analysis model in the time domain based on the acceleration unit-impulse response matrix, in which several new solution techniques, such as a dimensionless method to solve the interaction force, are applied to improve the numerical stability of the actual soil parameters and reduce the amount of calculation. Finally, the feasibility of the time domain methods are illustrated by the response of the nuclear power structure and the accuracy of the algorithms are dynamically verified by comparison with the refinement of a large-scale viscoelastic soil model.
文摘Studies on nonlinear behavior at oil/water interface membrane were performed. This system showed rhythmic oscillations and chaos of electrical potential in a given concentration domain. The nonlinear behavior response at the liquid membrane apparently resembled that of biological chemoreceptive membrane. The possibility of developing a new type of chemical sensor with the ability to simulate substance equilibrium in living organisms was suggested in the paper.
基金supported by the National Nature Science Foundation Project(Nos.41604101 and U1562215)the National Grand Project for Science and Technology(No.2016ZX05024-004)+2 种基金the Natural Science Foundation of Shandong(No.BS2014NJ005)Science Foundation from SINOPEC Key Laboratory of Geophysics(No.33550006-15-FW2099-0027)the Fundamental Research Funds for the Central Universities
文摘Amplitude variations with offset or incident angle (AVO/AVA) inversion are typically combined with statistical methods, such as Bayesian inference or deterministic inversion. We propose a joint elastic inversion method in the time and frequency domain based on Bayesian inversion theory to improve the resolution of the estimated P- and S-wave velocities and density. We initially construct the objective function using Bayesian inference by combining seismic data in the time and frequency domain. We use Cauchy and Gaussian probability distribution density functions to obtain the prior information for the model parameters and the likelihood function, respectively. We estimate the elastic parameters by solving the initial objective function with added model constraints to improve the inversion robustness. The results of the synthetic data suggest that the frequency spectra of the estimated parameters are wider than those obtained with conventional elastic inversion in the time domain. In addition, the proposed inversion approach offers stronger antinoising compared to the inversion approach in the frequency domain. Furthermore, results from synthetic examples with added Gaussian noise demonstrate the robustness of the proposed approach. From the real data, we infer that more model parameter details can be reproduced with the proposed joint elastic inversion.
基金Supported by Grants from the National Natural Science Foundation of China, No. 39970340the Scientific Fund of the Chinese Ministry of Health, 98-2-283the Natural Science Foundation of Shanghai,No. 02ZB14041 and 034119916
文摘AIM: To investigate the role of P-selectin, intercellular adhesion molecule-1 (ICAM-1) and dendritic cells (DCs)in liver/kidney of rats with hepatic/renal ischemiareperfusion injury and the preventive effect of anti-Pselectin lectin-EGF domain monoclonal antibody (anti-PsLEGFmAb) on the injury.METHODS: Rat models of hepatic and renal ischemiareperfusion were established. The rats were then divided into two groups, one group treated with anti-PsL-EGFmAb(n = 20) and control treated with saline (n = 20). Both groups were subdivided into four groups according to reperfusion time (1, 3, 6 and 24 h). The sham-operated group (n = 5) served as a control group. DCs were observed by the microscopic image method, while P-selectin and ICAM-1 were analyzed by immunohistochemistry.RESULTS: P-selectin increased significantly in hepatic sinusoidal endothelial cells and renal tubular epithelial cells 1 h after ischemia-reperfusion, and the expression of ICAM-1 was up-regulated in hepatic sinusoid and renal vessels after 6 h. CD1a+CD80+DCs gradually increased in hepatic sinusoidal endothelium and renal tubules and interstitium 1 h after ischemia-reperfusion, and there was the most number of DCs in 24-h group. The localization of DCs was associated with rat hepatic/renal function.These changes became less significant in rats treated with anti-PsL-EGFmAb.CONCLUSION: DCs play an important role in immune pathogenesis of hepatic/renal ischemia-reperfusion injury.Anti-PsL-EGFmAb may regulate and inhibit local DC immigration and accumulation in liver/kidney.
基金supported by the National Key Project of Fundamental Research of China(Grant No.2012CB932304)the National Natural Science Foundation of China(Grant No.50831006)+1 种基金the Program for New Century Excellent Talents in University(Grant No.NCET-11-0156)the Priority Academic Program Development of Jiangsu Higher Education Institutions
文摘Ferromagnetic shape memory Ni-Mn-Ga films with 7M modulated structure were prepared on MgO (001) substrates by magnetron sputtering. Magnetization process with a typical two-hysteresis loop indicates the occurrence of the reversible magnetic field-induced reorientation. Magnetic domain structure and twin structure of the film were controlled by the in- terplay of the magnetic and temperature field. With cooling under an out-of-plane magnetic field, the evolution of magnetic domain structure reveals that martensitic transformation could be divided into two periods: nucleation and growth. With an in-plane magnetic field applied to a thermomagnetic-treated film, the evolution of magnetic domain structure gives evidence of a reorientation of twin variants of martensite. A microstructural model is described to define the twin structure and to produce the magnetic domain structure at the beginning of martensitic transformation; based on this model, the relationship between the twin structure and the magnetic domain structure for the treated film under an in-plane field is also described.
文摘Plant cellulose synthases (CesAs) are the key enzymes necessary for cellulose biosynthesis. In Arabidopsis, two distinct groups of three CesAs each are necessary for cellulose synthesis during primary and secondary cell wall formation. It has also been suggested that such three CesAs interact with each other to form plasma-membrane bound rosette complexes that are functional during cellulose production. However, in vivo demonstration of such assemblies of three CesAs into rosettes has not been possible. We used yeast two-hybrid assays to demonstrate the possible interactions among several CesAs from Arabidopsis and aspen via their N-terminal zinc-binding domains (ZnBDs). While strong positive interactions were detected among ZnBDs from secondary wall associated CesAs of both Arabidopsis and aspen, the intergeneric interactions between Arabidopsis and aspen CesAs were weak. Moreover, in aspen, three primary wall associated CesA ZnBDs positively interacted with each other as well as with secondary CesAs. These results suggest that ZnBDs from either primary or secondary CesAs, and even from different plant species could interact but are perhaps insufficient for specificities of such interactions among CesAs. These observations suggest that some other more specific interacting regions might exist within CesAs. It is also possible that some hitherto unknown mechanism exists in plants for assembling the rosette complexes with different compositions of CesAs. Understanding how cellulose is synthesized will have a direct impact on utilization of lignocellulosic biomass for bioenergy production.
基金The National Natural Science Foundation of China(No.51975117)。
文摘A modified time domain interpolation method is proposed for orthogonal frequency division multiplexing(OFDM)systems to address the problem that time domain interpolation in the least square(LS)channel estimation method based on comb-type pilots cannot choose the pilot spacing flexibly.Firstly,the estimated channel frequency response(CFR)at pilot positions in the frequency domain is obtained by LS channel estimation based on comb-type pilots,and the estimated channel impulse response(CIR)in the time domain is obtained by linear interpolation and inverse fast Fourier transform(IFFT).Secondly,the error of the estimated CIR obtained by linear interpolation is analyzed by theoretical deduction,and a method for correcting it is proposed.Finally,an estimated CFR at all subcarrier positions in the frequency domain is obtained by performing zero padding in the time domain and fast Fourier transform(FFT)on the modified CIR.The simulation results suggest that the proposed method gives similar performance to time domain interpolation,yet it does not need to meet the condition of time domain interpolation that the number of subcarriers must be an integral multiple of pilot spacing to use it.The proposed method allows for flexible pilot spacing,reducing the number of pilots and the consumption of subcarriers used for channel estimation.
基金Project(2018YFC0807802)supported by the National Key R&D Program of ChinaProject(41874081)supported by the National Natural Science Foundation of China
文摘Due to the strong electromagnetic interferences and human interference,traditional electromagnetic methods cannot obtain high quality resistivity data of mineral deposits in Chinese mines.The wide field electromagnetic method(WFEM),in which the pseudo-random signal is taken as the transmitter source,can extract high quality resistivity data in areas with sever interference by only measuring the electric field component.We use the WFEM to extract the resistivity information of the Dongguashan mine in southeast China.Compared with the audio magnetotelluric(AMT)method,and the controlled source audio-frequency magnetotelluric(CSAMT) method,the WFEM can obtain data with higher quality and simpler operations.The inversion results indicate that the WFEM can accurately identify the location of the main ore-body,which can be used for deep mine exploration in areas with strong interference.
文摘In this paper we investigate simultaneous approximation for arbitrary system of nodes on smooth domain in complex plane. Some results which are better than those of known theorems are obtained.
文摘In this paper, a 3D time domain technique is adopted to calculate the coupled hydrodynamic interaction between two bodies without flare in waves. For verifying the code, two same cylinders are selected to calculate coupled hydrodynamic effects by comparison with the results obtained by 3D frequency method which has been proved to be efficient for solving such problems. In order to improve efficiency of calculation, the effect of history time has been discussed, and an improved method is presented. Moreover, the effect of lateral separation distance is also discussed in detail. The technique developed here may serve as a more rigorous tool to analyze the related transient problems of two ships doing underway replenishment in waves.
基金P.Sun was supported by NSF Grant DMS-1418806C.S.Zhang was partially supported by the National Key Research and Development Program of China(Grant No.2016YFB0201304)+1 种基金the Major Research Plan of National Natural Science Foundation of China(Grant Nos.91430215,91530323)the Key Research Program of Frontier Sciences of CAS.
文摘The distributed Lagrange multiplier/fictitious domain(DLM/FD)-mixed finite element method is developed and analyzed in this paper for a transient Stokes interface problem with jump coefficients.The semi-and fully discrete DLM/FD-mixed finite element scheme are developed for the first time for this problem with a moving interface,where the arbitrary Lagrangian-Eulerian(ALE)technique is employed to deal with the moving and immersed subdomain.Stability and optimal convergence properties are obtained for both schemes.Numerical experiments are carried out for different scenarios of jump coefficients,and all theoretical results are validated.