China is currently the world＇s leading producer of Lentinula edodes and owns many cultivated strains of this species. This study was performed in order to investigate intergenic spacer I （IGS1） polymorphism and cla...China is currently the world＇s leading producer of Lentinula edodes and owns many cultivated strains of this species. This study was performed in order to investigate intergenic spacer I （IGS1） polymorphism and classification among 49 popular cultivated strains. The great majority of the 49 strains possessed two different IGS1 sequences, with distinct lengths and homologies. Based on the length and homology of the IGS1 sequences of the 49 strains, the strains were classified into two groups： A and B. Group A was subdivided into six subgroups. Forty-seven strains were homozygous or heterozygous among these six subgroups in group A, Cr01 was heterozygous between A and B, and Guangxiang 9 was homozygous in group B. An IGS1 polymorphism map of each cultivated L. edodes strain is reported for the first time and could be used as a marker for the initial classification and management of cultivated L. edodes strains in China.展开更多
Phylogenetic relationships of Arundinaria and related genera (Pleioblastus, Pseudosasa, Oligostachyum, Bashania, Clavinodum, etc.) were assessed by analyzing the sequences of the nrDNA internal transcribed spacer (ITS...Phylogenetic relationships of Arundinaria and related genera (Pleioblastus, Pseudosasa, Oligostachyum, Bashania, Clavinodum, etc.) were assessed by analyzing the sequences of the nrDNA internal transcribed spacer (ITS) and the cpDNA trnL-F intergenic spacer (IGS). Comparison with trnL-F IGS sequence, the ITS region provided the higher number of parsimony informative characters, and the interspecific variation of the ITS sequence was higher than that of the trnL-F IGS sequence.The tree obtained by combining both sets of data showed that the species sampled in Arundinaria and the related genera were monophyletic and divided into two clades. The relationships and positioning of all the taxa surveryed (including A. oleosa, A. hsienchuensis, A. chino, A. amara, A. yixingensis, A. amabilis, A. fortunei, A. pygmaea, A. gramineus, A. fargesii, A. faberi, A. hupehense, Pseudosasa japonica cv. Tsutsumiana, P. japonica and Brachystachyum densiflorum) were also discussed. The results from the sequences were broadly consistent with morphological characters, appearing all these taxa sampled belong to the genus of Arundinaria. The topologies of the trees generated from individual data and the combined data were similar.展开更多
An improved method of PCR in which the small segment of conchocelis is amplified directly without DNA extraction was used to amplify a RUBISCO intergenic spacer DNA fragment from nine species of red algal genus Porphy...An improved method of PCR in which the small segment of conchocelis is amplified directly without DNA extraction was used to amplify a RUBISCO intergenic spacer DNA fragment from nine species of red algal genus Porphyra(Bangiales,Rhodophyta),including Porphyra yezoensis(Jiangsu,China),P.haitanensis(Fujian,China),P.oligospermatangia(Qingdao,China),P.katadai(Qingdao,China),P.tenera(Qingdao,China),P.suborboculata(Fujian,China),P.pseudolinearis(Kogendo,Korea),P.linearis(Devon,England),and P.fallax(Seattle,USA).Standard PCR and the method developed here were both conducted using primers specific for the RUBISCO spacer region,after which the two PCR products were sequenced.The sequencing data of the amplicons obtained using both methods were identical,suggesting that the improved PCR method was functional.These findings indicate that the method developed here may be useful for the rapid identification of species of Porphyra in a germplasm bank.In addition,a phylogenetic tree was constructed using the RUBISCO spacer and partial rbcS sequence,and the results were in concordant with possible alternative phylogenies based on traditional morphological taxonomic characteristics,indicating that the RUBISCO spacer is a useful region for phylogenetic studies.展开更多
以采自云南地区的5个牛肝菌（Boletus sp.）样品为材料,根据样品的形态特征,并结合r DNA ITS和IGS1序列分析对5个样品进行分类鉴定,通过与Gen Bank上已登录的牛肝菌序列比对,采用邻接法（neighbor-joining,N-J）构建系统发育树。结果表...以采自云南地区的5个牛肝菌（Boletus sp.）样品为材料,根据样品的形态特征,并结合r DNA ITS和IGS1序列分析对5个样品进行分类鉴定,通过与Gen Bank上已登录的牛肝菌序列比对,采用邻接法（neighbor-joining,N-J）构建系统发育树。结果表明,所有供试材料的ITS和IGS1全长分别在739~787bp和537~583bp范围内,GC含量分别在48.31%~51.01%和48.42%~52.89%之间,遗传距离在0.017~0.248和0.002~0.225之间,上述数据表明云南5个牛肝菌样品之间存在一定的遗传差异。5个牛肝菌样品的ITS和IGS1序列聚类分析均表明,供试样品被分为两大类,其中样品YX1-2和QJ3-4聚为一个类群,样品CX2-10、QJ3-5和YX1-3聚为另一个大的类群,两种分析手段相互印证,且ITS序列聚类分析能将云南不同地点的牛肝菌鉴定到属甚至种的水平,上述分析结果表明ITS和IGS1序列分析相结合可作为牛肝菌亲缘关系鉴定的有效分子标记方法。展开更多
基金supported by the earmarked fund for China Agriculture Research System (CARS-20)the Youth Talent Development Plan of Shanghai Municipal Agricultural System, China (20160113)the Agriculture Research System of Shanghai, China (201809)
文摘China is currently the world＇s leading producer of Lentinula edodes and owns many cultivated strains of this species. This study was performed in order to investigate intergenic spacer I （IGS1） polymorphism and classification among 49 popular cultivated strains. The great majority of the 49 strains possessed two different IGS1 sequences, with distinct lengths and homologies. Based on the length and homology of the IGS1 sequences of the 49 strains, the strains were classified into two groups： A and B. Group A was subdivided into six subgroups. Forty-seven strains were homozygous or heterozygous among these six subgroups in group A, Cr01 was heterozygous between A and B, and Guangxiang 9 was homozygous in group B. An IGS1 polymorphism map of each cultivated L. edodes strain is reported for the first time and could be used as a marker for the initial classification and management of cultivated L. edodes strains in China.
文摘Phylogenetic relationships of Arundinaria and related genera (Pleioblastus, Pseudosasa, Oligostachyum, Bashania, Clavinodum, etc.) were assessed by analyzing the sequences of the nrDNA internal transcribed spacer (ITS) and the cpDNA trnL-F intergenic spacer (IGS). Comparison with trnL-F IGS sequence, the ITS region provided the higher number of parsimony informative characters, and the interspecific variation of the ITS sequence was higher than that of the trnL-F IGS sequence.The tree obtained by combining both sets of data showed that the species sampled in Arundinaria and the related genera were monophyletic and divided into two clades. The relationships and positioning of all the taxa surveryed (including A. oleosa, A. hsienchuensis, A. chino, A. amara, A. yixingensis, A. amabilis, A. fortunei, A. pygmaea, A. gramineus, A. fargesii, A. faberi, A. hupehense, Pseudosasa japonica cv. Tsutsumiana, P. japonica and Brachystachyum densiflorum) were also discussed. The results from the sequences were broadly consistent with morphological characters, appearing all these taxa sampled belong to the genus of Arundinaria. The topologies of the trees generated from individual data and the combined data were similar.
基金Supported by the National High Technology Research and Development Program of China (863 Program)(No 2006AA10A402)Project for Supporting National Development (No 2006BAD09A04)+2 种基金the National Natural Science Foundation of China (Nos U0633006,40476059)the Natural Science Foundation of Qingdao (No 05-2-p-2)the Knowledge Innovation Program of the Chinese Academy of Sciences (No KZCX2-211)
文摘An improved method of PCR in which the small segment of conchocelis is amplified directly without DNA extraction was used to amplify a RUBISCO intergenic spacer DNA fragment from nine species of red algal genus Porphyra(Bangiales,Rhodophyta),including Porphyra yezoensis(Jiangsu,China),P.haitanensis(Fujian,China),P.oligospermatangia(Qingdao,China),P.katadai(Qingdao,China),P.tenera(Qingdao,China),P.suborboculata(Fujian,China),P.pseudolinearis(Kogendo,Korea),P.linearis(Devon,England),and P.fallax(Seattle,USA).Standard PCR and the method developed here were both conducted using primers specific for the RUBISCO spacer region,after which the two PCR products were sequenced.The sequencing data of the amplicons obtained using both methods were identical,suggesting that the improved PCR method was functional.These findings indicate that the method developed here may be useful for the rapid identification of species of Porphyra in a germplasm bank.In addition,a phylogenetic tree was constructed using the RUBISCO spacer and partial rbcS sequence,and the results were in concordant with possible alternative phylogenies based on traditional morphological taxonomic characteristics,indicating that the RUBISCO spacer is a useful region for phylogenetic studies.
文摘以采自云南地区的5个牛肝菌（Boletus sp.）样品为材料,根据样品的形态特征,并结合r DNA ITS和IGS1序列分析对5个样品进行分类鉴定,通过与Gen Bank上已登录的牛肝菌序列比对,采用邻接法（neighbor-joining,N-J）构建系统发育树。结果表明,所有供试材料的ITS和IGS1全长分别在739~787bp和537~583bp范围内,GC含量分别在48.31%~51.01%和48.42%~52.89%之间,遗传距离在0.017~0.248和0.002~0.225之间,上述数据表明云南5个牛肝菌样品之间存在一定的遗传差异。5个牛肝菌样品的ITS和IGS1序列聚类分析均表明,供试样品被分为两大类,其中样品YX1-2和QJ3-4聚为一个类群,样品CX2-10、QJ3-5和YX1-3聚为另一个大的类群,两种分析手段相互印证,且ITS序列聚类分析能将云南不同地点的牛肝菌鉴定到属甚至种的水平,上述分析结果表明ITS和IGS1序列分析相结合可作为牛肝菌亲缘关系鉴定的有效分子标记方法。
