Cloning of Hoxc8 Promoter in Mongolian Sheep by Thermal Asymmetric Interlaced PCR

[Objective] The research aimed toexplore the method to obtain Hoxc8 pro- moter of Mongolian Sheep. [Method] Thermal asymmetric interlaced PCR was used to amplify the promoter sequence of Hoxc8 inMongolian Sheep. [Result] The ob- tained sequence by usingthermal asymmetric interlaced PCRwas not ideal and the sequencing results were not matching to the known sequence. Though promoter se- quence of Hoxc8 in Mongolian Sheep was not obtained by thermal asymmetric in- terlaced PCR, but the results could provide references for the relevant studies in the future. [Conclusion] The research laid the foundation for further study on the methy- lation status Hoxc8 promoter in Mongolian Sheep.

Full‑Fiber Auxetic‑Interlaced Yarn Sensor for Sign‑Language Translation Glove Assisted by Artificial Neural Network

Yarn sensors have shown promising application prospects in wearable electronics owing to their shape adaptability, good flexibility, and weavability. However, it is still a critical challenge to develop simultaneously structure stable, fast response, body conformal, mechanical robust yarn sensor using full microfibers in an industrial-scalable manner. Herein, a full-fiber auxetic-interlaced yarn sensor(AIYS) with negative Poisson’s ratio is designed and fabricated using a continuous, mass-producible, structure-programmable, and low-cost spinning technology. Based on the unique microfiber interlaced architecture, AIYS simultaneously achieves a Poisson’s ratio of-1.5, a robust mechanical property(0.6 c N/dtex), and a fast train-resistance responsiveness(0.025 s), which enhances conformality with the human body and quickly transduce human joint bending and/or stretching into electrical signals. Moreover, AIYS shows good flexibility, washability, weavability, and high repeatability. Furtherly, with the AIYS array, an ultrafast full-letter sign-language translation glove is developed using artificial neural network. The sign-language translation glove achieves an accuracy of 99.8% for all letters of the English alphabet within a short time of 0.25 s. Furthermore, owing to excellent full letter-recognition ability, real-time translation of daily dialogues and complex sentences is also demonstrated. The smart glove exhibits a remarkable potential in eliminating the communication barriers between signers and non-signers.

Methods for obtaining characteristic γ-ray net peak count from interlaced overlap peak in HPGe γ-ray spectrometer system

For a characteristic c-ray with interlaced overlap peak, and the case where its reliable and credible net count cannot be obtained using the current high-purity germanium(HPGe) multichannel γ-ray spectrum software, two new methods are proposed herein to obtain the γ-ray net peak count from the interlaced overlap peak in the HPGe cray spectrometer system, of which one is the symmetric conversion method based on Gaussian distribution and the other is where the energy average value of two close γ-rays is regarded as the γ-ray energy. The experimental results indicate that the two methods mentioned above are reliable and credible. This study is significant for the development of better γ-ray spectrum processing software for measuring complex γ-ray spectra concerning the nuclear reaction cross section, neutron activation analysis, and analysis of transuranium elements, using an HPGe detector.

Near-wellbore fracture propagation physical simulation based on innovative interlaced fixed perforation

An innovative perforation method of interlaced fixed perforation was put forward based on the analysis of the characteristics of fractures in various periods of perforation and conventional perforation modes.By conducting a large-scale perforation shooting experiments,we investigated the morphology,propagation mechanism and propagation law of the near-wellbore fractures generated during perforating processes under different fixed angle and interlaced angle combinations,and discussed the control method of near-wellbore fractures in different types of unconventional oil and gas reservoirs.The experimental results show that:(1)The interlaced fixed perforation strengthens the connectivity between the perforation tunnels not only in the same fixed plane but also in adjacent fixed planes,making it likely to form near-wellbore connected fractures which propagate in order.(2)Three kinds of micro-fractures will come up around the perforation tunnel during perforation,namely typeⅠradial micro-fracture,typeⅡoblique micro-fracture and typeⅢdivergent micro-fracture at the perforation tip,which are interconnected into complex near-wellbore fracture system.(3)Different types of perforation bullets under different combinations of fixed angles and interlaced angles result in different shapes of near-wellbore fractures propagating in different patterns.(4)By using the interlaced perforation on fixed planes,arranging fixed planes according to the spiral mode or the continuous"zigzag"shape,the desired near-wellbore fractures can be obtained,which is conducive to the manual control of main fractures in the fracturing of unconventional or complex conventional reservoirs.

Compact Interlaced Dual Circularly Polarized Sequentially Rotated Dielectric-Resonator Antenna Array

In this study,a compact 2×2 interlaced sequentially rotated dual-polarized dielectric-resonator antenna array is proposed for 5.8 GHz applications.The array is composed of a novel unit elements that are made of rectangular dielectric resonator(RDR)coupled to an eye slot for generating the orthogonal modes,TEδx 21 and TE1yδ1 to acquire circular polarization(CP)radiation.For the purpose of miniaturization and achieving dual polarized resonance,the array is fed by two interlaced ports and each port excites two radiating elements.The first port feeds horizontal elements to obtain left hand circular polarization(LHCP).The second port feeds vertical elements to obtain right hand circular polarization(RHCP).A quarter-wave length transformer is employed to reduce the attenuation and consequently increase the array gain performance.The 35×35 mm2(0.676λ0×0.676λ0)gains were 8.4 and 8.2 dBi for port 1 and port 2,respectively,with port isolations of−33.51 dB.The design achieves a voltage standing-wave ratio(VSWR)<−10 dB and an axial ratio(AR)<−3 dB bandwidth of 2.48%(5.766 to 5.911 GHz)for LHCP at port 1 and a VSWR<−10 dB and AR<−3 dB bandwidth of 2.28%(5.788 to 5.922 GHz)for RHCP at port 2.The findings of the proposed design validate its use for ISM band applications.

Using Self-referencing Interlaced Submatrices to Determine the Number of Chemical Species in a Mixture

Determining the number of chemical species is the first step in analyses of a chemical or biological system. A novel method is proposed to address this issue by taking advantage of frequency differences between chemical information and noise. Two interlaced submatrices were obtained by downsampling an original data spectra matrix in an interlacing manner. The two interlaced submatrices contained similar chemical information but different noise levels. The number of relevant chemical species was determined through pairwise comparisons of principal components obtained by principal component analysis of the two interlaced submatrices. The proposed method, referred to as SRISM, uses two self-referencing interlaced submatrices to make the determination. SRISM was able to selectively distinguish relevant chemical species from various types of interference factors such as signal overlapping, minor components and noise in simulated datasets. Its performance was further validated using experimental datasets that contained high-levels of instrument aberrations, signal overlapping and collinearity. SRISM was also applied to infrared spectral data obtained from atmospheric monitoring. It has great potential for overcoming various types of interference factor. This method is mathematically rigorous, computationally efficient, and readily automated.

Effect of Processing Conditions on the Interlaced Yarn

A new interlacer is used in this paper to investigate the effect of processing conditions on the properties of interlaced yarn. The experimental results show that number of tangles of interlaced yarn changes little with air pressure from 0.2 MPa to 0.5 MPa, and the number of tangles has a maximum value with yarn speed changing. For two yarn directions of entering and leaving yarn guide of interalcer, the yarn speed for the maximum number of tangles is 400 m/rain and 600 m/rain, respectively. The number of tangles changes with air pressure and yarn speed for two yarn directions is compared.

Exploiting MCF-7 Cells’ Calcium Dependence with Interlaced Therapy

The purpose of this study is to demonstrate MCF-7 cells’ dependence on calcium for growth and to exploit that dependence to improve chemotherapy efficacy. Fura-2 fluorescence imaging shows that MCF-7 cells maintain a higher basal intracellular calcium concentration than non-tumorigenic MCF-10A cells. Blocking T-type calcium channels with mibefradil reduced MCF-7 intracellular calcium concentration. Flow cytometry shows that knocking down T-type calcium channel expression with siRNA caused an increase in MCF-7 cells in G1 phase and a decrease in cells in S phase. Proliferation assays of MCF-7 cells treated with EGTA and thapsigargin reveal the dependence of MCF-7 cell growth on extracellular and intracellular calcium sources, respectively. In vitro, interlaced treatment that alternated the T-type calcium channel blocker NNC-55-0396 with paclitaxel more effectively reduced MCF-7 cell number than chemotherapy alone. In a mouse in vivo model, interlaced mibefradil and paclitaxel more effectively reduced MCF-7 xenograft size than chemotherapy alone. These findings indicate that MCF-7 cells are dependent on calcium for proliferation, particularly in passing the G1/S cell cycle checkpoint. Further, this dependence on calcium can be exploited by alternating treatment with T-type calcium channel blockers with paclitaxel in an interlaced therapy scheme that increases the efficacy of the chemotherapy.

A Probabilistic Paraconsistent Logical Model for Non-Relativistic Quantum Mechanics Using Interlaced Bilattices with Conflation and Bernoulli Distribution

In this work, we make a representation of non-relativistic quantum theory based on foundations of paraconsistent annotated logic (PAL), a propositional and evidential logic with an associated lattice FOUR. We use the PAL version with annotation of two values (PAL2v), named paraquantum logic (PQL), where the evidence signals are normalized values and the intensities of the inconsistencies are represented by degrees of contradiction. Quantum mechanics is represented through mapping on the interlaced bilattices where this logical formalization allows annotation of two values in the format of degrees of evidence of probability. The Bernoulli probability distribution is used to establish probabilistic logical states that identify the superposition of states and quantum entanglement with the equations and determine the state vectors located inside the interlaced Bilattice. In the proposed logical probabilistic paraquantum logic model (pPQL Model), we introduce the operation of logical conflation into interlaced bilattice. We verify that in the pPQL Model, the operation of logical conflation is responsible for providing a suitable model for various phenomena of quantum mechanics, mainly the quantum entanglement. The results obtained from the entanglement equations demonstrate the formalization and completeness of paraquantum logic that allows for interpretations of similar phenomena of quantum mechanics, including EPR paradox and the wave-particle theory.

A Comparative Analysis for Land Utilization: Steel and R/C Interlaced Structures

In architecture,interlace structural concept is considered as a new design approach for cosmopolitan cities with high density to minimize the land use and increase the interaction.With various architectural approach,land resources can be minimized by this interlace concept for residential complexes.Such buildings will eliminate the reduction of land resource problem and on the other side safety measures in structural design is incorporated by interlace concept of buildings.This new concept can be constructed steel or reinforced concrete.In this paper,an analytical approach has been presented for these buildings in architecture and structural design.In the research,design considerations were taken for interlaced structures with reinforced concrete and steel.Components of steel structure,isolated footing,and columns.This paper is presenting a step wise process for interlaced structures.They are identification of project area,layout and model preparation,analysis and design of concrete interlaced structure,analysis and design of steel interlaced structure,drafting of the plans and costing and estimation of the structures.Comparison of both reinforced concrete and steel structures were carried out.The main aim of the paper is to provide a comparison between steel and concrete interlaced structure.A cost estimation was carried out to determine optimum design and construction for interlaced structures.

Interlaced optimal-REQUEST and unscented Kalman filtering for attitude determination

Aimed at low accuracy of attitude determination because of using low-cost components which may result in non-linearity in integrated attitude determination systems, a novel attitude determination algorithm using vector observations and gyro measurements is presented. The various features of the unscented Kalman filter （UKF） and optimal-REQUEST （quaternion estimator） algorithms are introduced for attitude determination. An interlaced filtering method is presented for the attitude determination of nano-spacecraft by setting the quaternion as the attitude representation, using the UKF and optimal-REQUEST to estimate the gyro drifts and the quaternion, respectively. The optimal-REQUEST and UKF are not isolated from each other. When the optimal-REQUEST algorithm estimates the attitude quaternion, the gyro drifts are estimated by the UKF algorithm synchronously by using the estimated attitude quaternion. Furthermore, the speed of attitude determination is improved by setting the state dimension to three. Experimental results show that the presented method has higher performance in attitude determination compared to the UKF algorithm and the traditional interlaced filtering method and can estimate the gyro drifts quickly.

A low-power DCO using inverter interlaced cascaded delay cell

This paper presents a low-power small-area digitally controlled oscillator （DCO） using an inverters interlaced cascaded delay cell （IICDC）. It uses a coarse-fine architecture with binary-weighted delay stages for the delay range and resolution. The coarse-tuning stage of the DCO uses IICDC, which is power and area efficient with low phase noise, as compared with conventional delay cells. The ADPLL with a DCO is fabricated in the UMC 180-nm CMOS process with an active area of 0.071 mm2. The output frequency range is 140-600 MHz at the power supply of 1.8 V. The power consumption is 2.34 mW@ a 200 MHz output.

Molecular Cloning and Characterization of a Novel Gene Involved in Fatty Acid Synthesis in Brassica napus L.

Based on the sequence of a novel expressed sequence tag （EST）, the full-length cDNA of 1 017 nucleotides was cloned from Brassica napus cv. Xiangyou 15 through rapid amplification of cDNA ends （RACE）. The gene was designated as Bnhol34 （HQ585980）, encoding a protein of 338 amino acids. BLAST analysis showed no high degree of sequence identity to any known gene. The calculated molecular weight of the Bnhol34 protein was 36.23 kDa, and the theoretical isoelectric point was 8.74. The Bnhol34 was also cloned from a high oleic acid mutant 854-1 through homologous cloning. There was no difference between the two Bnhol34 genes. Bnhol34 was localized in a tissue-specific manner in B. napus, and its expression level was about eight-fold greater in Xiangyou 15 seeds than in 854-1. The promoter region sequences of Bnhol34 were then isolated from Xiangyou 15 and 854-1, and a 93-bp deletion was found to occur in the Bnhol34 promoter region of 854-1. Three abscisic acid-responsive cis-elements （ABRE） were identified in the promoter region of Xiangyou 15. Real-time PCR analyses revealed that exogenous abscisic acid increased Bnhol34 expression by about four-fold in Xiangyou 15 seeds, yet did not change Bnhol34 expression in 854-1. It appeared that Bnhol34 might be abscisic acid insensitive in 854-1.

Solvability Condition for Robust Stabilization Problem of Control Systems with Parameter Uncertainties

Aim The solvability condition for robust stabilization problem associated with a plant family P(s,δ) having parameter uncertainty δ was considered. Methods Using Youla parameterization of the stabilizers this problem was transformed into a strong stabilization problem associated with a related plant family G (s, δ). Results A necessary solvability condition was established in terms of the parity interlacing property of each element in G(s,δ). Another apparently necessary solvability condition is that every element in P(s,δ) must be stabilizable. Conclusion The two conditions will be compared with each other and it will be shown that every element in G(s,δ) possesses parity interlacing property if P(s,δ) is stabilizable.

Improving breakdown voltage performance of SOI power device with folded drift region

A novel silicon-on-insulator（SOI） high breakdown voltage（BV） power device with interlaced dielectric trenches（IDT） and N/P pillars is proposed. In the studied structure, the drift region is folded by IDT embedded in the active layer,which results in an increase of length of ionization integral remarkably. The crowding phenomenon of electric field in the corner of IDT is relieved by the N/P pillars. Both traits improve two key factors of BV, the ionization integral length and electric field magnitude, and thus BV is significantly enhanced. The electric field in the dielectric layer is enhanced and a major portion of bias is borne by the oxide layer due to the accumulation of inverse charges（holes） at the corner of IDT.The average value of the lateral electric field of the proposed device reaches 60 V/μm with a 10 μm drift length, which increases by 200% in comparison to the conventional SOI LDMOS, resulting in a breakdown voltage of 607 V.

Commentary of Wind and Window Flower by Robert Frost

Wind and Window Flower is a special metaphorical love poem by Robert Frost. This tale of love actually presents a love journey of temptation, excitement, and disappointment. This paper tries to analysis the poem from the perspective of the sound of the poetry, the metro and rhyme, the conceptual and rhetorical.

基于Kalman预测的自适应De-interlace算法

本文提出一种基于Kalman预测的自适应De-interlace算法。它包括运动块检测、自适应的运动估计以及对运动块的运动补偿和对静止块的场复制等模块。其中,运动块检测用了连续的4场图像,能够更精确地实现运动检测;对检测到的运动块用Kalman滤波进行自适应地运动估计,并根据估计到的运动矢量进行运动补偿;对静止块,用场复制法进行De-interlace处理。该算法在de-interlace性能接近全搜索算法的前提下,运算量降低了三个数量级。

Kagome Project:Physical and Numerical Modeling Comparison for a Post-formed Elastic Gridshell

An elastic gridshell is an efficient constructive typology for crossing large spans with little material.A flat elastic grid is built before buckling the structure into shape,in active and post-formed bending.The design and structural analysis of such a structure requires a stage of form finding that can mainly be done:(1)With a physical model:either by a suspended net method,or an active bending model;(2)With a numerical model performed by dynamic relaxation.All these solutions have various biases and assumptions that make them reflect more or less the reality.These three methods have been applied by Happold and Liddell[1]during the design of the Frei Otto’s Mannheim Gridshell which has allowed us to compare the results,and to highlight the significant differences between digital and physical models.Based on our own algorithm called ELASTICA[2],our study focuses on:(1)Comparing the results of the ELASTICA’s numerical models to load tests on physical models;(2)The identification of the various factors that can influence the results and explain the observed differences,some of which are then studied;(3)Applying the results to build a full-scale interlaced lattice elastic gridshell based on the Japanese Kagome pattern.

解码MPEG4视频流中interlace宏块的原理和实现方法

在 MPEG4中 interlace可以做到宏块级。当宏块使用 interlace的方式进行编码的时候 ,可以结合 dct_ type,field_ prediction,forword_ top_ field_ reference,forword_ bot_ field_ reference等标志来确定 interlace宏块的解码方法。

THEORETICAL CALCULATION OF SPECIFIC INTERFACIALENERGY OF SEMICOHERENT INTERFACE BETWEEN MICROALLOY CARBONITRIDES AND AUSTENITE

According to the misfitting dislocation theory,a method of theoretical calculation was devel- oped for the specific energy of the semicoherent interface between microalloy carbonitrides and austenite matrix.The calculating formulae were derived and the results were satisfactorily applied on the research works.