Background： Interleukin-1β（IL-1β） is important mediator of inflammatory-induced suppression of reproductive axis at the hypothalamic level. At the beginning of inflammation, the main source of cytokines in the ce...Background： Interleukin-1β（IL-1β） is important mediator of inflammatory-induced suppression of reproductive axis at the hypothalamic level. At the beginning of inflammation, the main source of cytokines in the cerebrospinal fluid（CSF） is peripheral circulation, while over time, cytokines produced in the brain are more important. Melatonin has been shown to decrease pro-inflammatory cytokines concentration in the brain. In ewes, melatonin is used to advance the onset of a breading season. Little is known about CSF concentration of IL-1β in ewes and its correlation with plasma during inflammation as well as melatonin action on the concentration of IL-1β in blood plasma and the CSF, and brain barriers permeability in early stage of lipopolysaccharide（LPS）-induced inflammation.Methods： Systemic inflammation was induced through LPS administration in melatonin-and sham-implanted ewes. Blood and CSF samples were collected before and after LPS administration and IL-1β and albumin concentration were measured. To assess the functions of brain barriers albumin quotient（QAlb） was used.Expression of IL-1β（Il1B） and its receptor type Ⅰ（Il1r1） and type Ⅱ（Il1r2） and matrix metalloproteinase（Mmp） 3 and 9 was evaluated in the choroid plexus（CP）.Results： Before LPS administration, IL-1β was on the level of 62.0 ± 29.7 pg/mL and 66.4 ± 32.1 pg/mL in plasma and 26.2 ± 5.4 pg/mL and 21.3 ± 8.7 pg/mL in the CSF in sham-and melatonin-implanted group, respectively.Following LPS it increased to 159.3 ± 53.1 pg/mL and 197.8 ± 42.8 pg/mL in plasma and 129.8 ± 54.2 pg/mL and139.6 ± 51.5 pg/mL in the CSF. No correlations was found between plasma and CSF IL-1β concentration after LPS in both groups. The QAlb calculated before LPS and 6 h after was similar in all groups. Melatonin did not affected m RNA expression of Il1B, Il1r1 and Il1r2 in the CP. The m RNA expression of Mmp3 and Mmp9 was not detected.Conclusions： The lack of correlation between plasma and CSF IL-1β concentration indicates that at the beginning of inflammation the local synthesis of IL-1β in the CP is an important source of IL-1β in the CSF. Melatonin from slow-release implants does not affect IL-1β concentration in plasma and CSF in early stage of systemic inflammation.展开更多
Objective: To determine if an amniotic fluid (AF)-specific marker is present and if its concentration changes with the presence of labor. Study Design: Twenty-six healthy women who gave birth to healthy newborns at te...Objective: To determine if an amniotic fluid (AF)-specific marker is present and if its concentration changes with the presence of labor. Study Design: Twenty-six healthy women who gave birth to healthy newborns at term during the period from July 2009 to January 2010 were included in the study. Six candidate markers were assessed by commercially available ELISA kits: interleukin (IL)-6, squamous cell carcinoma (SCC) antigen, insulin-like growth factor (IGFBP)-1, osteopontin (OPN), CA125, and sialyl Tn (STN). Results: The AF/maternal serum (MS) measurement based on IL-6 or SCC has proved to be superior to IGFBP-1, CA125, OPN and STN. Women with spontaneous labor at term had significantly higher IL-6 and IGFBP-1 concentrations in AF compared with those without labor. No significant differences were observed in the AF concentrations of SCC, OPN, CA125 and STN between women with labor and those not in labor. Conclusion: Our observation of IL-6 and SCC in AF may open a new area of research to assess their usefulness as biological markers of obstetrical disorders.展开更多
Interleukin-6 has been shown to be involved in nerve injury and nerve regeneration, but the effects of long-term administration of high concentrations of interleukin-6 on neurons in the central nervous system is poorl...Interleukin-6 has been shown to be involved in nerve injury and nerve regeneration, but the effects of long-term administration of high concentrations of interleukin-6 on neurons in the central nervous system is poorly understood. This study investigated the effects of 24 hour expo-sure of interleukin-6 on cortical neurons at various concentrations （0.1, 1, 5 and 10 ng/mL） and the effects of 10 ng/mL interleukin-6 exposure to cortical neurons for various durations （2, 4, 8, 24 and 48 hours） by studying voltage-gated Na＋ channels using a patch-clamp technique. Volt-age-clamp recording results demonstrated that interleukin-6 suppressed Na＋ currents through its receptor in a time- and dose-dependent manner, but did not alter voltage-dependent activation and inactivation. Current-clamp recording results were consistent with voltage-clamp recording results. Interleukin-6 reduced the action potential amplitude of cortical neurons, but did not change the action potential threshold. The regulation of voltage-gated Na＋channels in rat corti-cal neurons by interleukin-6 is time- and dose-dependent.展开更多
To investigate the levels of cytokines in subeschar edema fluid (SEF). Methods: A guinea pig model with III°30% total body surface area (TBSA) was designed. SEF was collected serially at the time of escharectomy ...To investigate the levels of cytokines in subeschar edema fluid (SEF). Methods: A guinea pig model with III°30% total body surface area (TBSA) was designed. SEF was collected serially at the time of escharectomy for up to 72 h after scald injury. The levels of tumor necrosis factor.(TNFα), interleukin-6 (IL-6) and interleukin-8 (IL-8) were determined by using the enzyme-linked immunosorbent assay (ELISA). Results: The levels of TNFα,IL-6 and IL-8 in plasma and SEF increased in the early phase of scald injury. Furthermore, the contents of TNFα， IL-6 and IL-8 in SEF were higher than those in plasma.Conclusion: The findings indicate that the subeschar edema fluid is a biologically active reservoir, and is an important participant in the postburn pathophysiologic process.展开更多
目的分析外伤性脑损伤（TBI）患者脑脊液及血清Nogo-A基因、白细胞介素1β（IL-1β）及白细胞介素6（IL-6）的表达。方法选取2012年10月~2015年5月江苏大学附属武进医院及江苏省宜兴市人民医院收治的70例TBI患者,按照格拉斯哥昏迷评分（...目的分析外伤性脑损伤（TBI）患者脑脊液及血清Nogo-A基因、白细胞介素1β（IL-1β）及白细胞介素6（IL-6）的表达。方法选取2012年10月~2015年5月江苏大学附属武进医院及江苏省宜兴市人民医院收治的70例TBI患者,按照格拉斯哥昏迷评分（GCS）分将其为轻度组（n=26）、中度组（n=21）及重度组（n=23）,分别于入院第1、4、7、10天采集其脑脊液及静脉血,比较各组患者脑脊液及血清Nogo-A m RNA、Nogo-A蛋白、IL-1β及IL-6表达的差异。结果随着伤情的加重及时间的增加,TBI患者脑脊液及血清中Nogo-A m RNA表达水平、NogoA蛋白浓度、IL-1β、IL-6均呈现上升趋势,伤后第4天达到峰值,并于受伤7 d后逐步下降（P〈0.05）。Pearson相关分析显示,脑脊液和血清中上述因子水平与患者的预后质量呈负相关,且差异有统计学意义（P〈0.05）。结论随着TBI严重程度的增加,患者脑脊液、血清Nogo-A基因、IL-1β、IL-6表达均显著增加,与患者的预后呈负相关,脑脊液内Nogo-A、IL-1β、IL-6表达水平与患者病情变化更为一致。展开更多
基金supported by a project funded by the National Science Centre allocated on the basis of decision-DEC 2011/03/B/NZ9/00118supported by Ministry of Science and High Education
文摘Background： Interleukin-1β（IL-1β） is important mediator of inflammatory-induced suppression of reproductive axis at the hypothalamic level. At the beginning of inflammation, the main source of cytokines in the cerebrospinal fluid（CSF） is peripheral circulation, while over time, cytokines produced in the brain are more important. Melatonin has been shown to decrease pro-inflammatory cytokines concentration in the brain. In ewes, melatonin is used to advance the onset of a breading season. Little is known about CSF concentration of IL-1β in ewes and its correlation with plasma during inflammation as well as melatonin action on the concentration of IL-1β in blood plasma and the CSF, and brain barriers permeability in early stage of lipopolysaccharide（LPS）-induced inflammation.Methods： Systemic inflammation was induced through LPS administration in melatonin-and sham-implanted ewes. Blood and CSF samples were collected before and after LPS administration and IL-1β and albumin concentration were measured. To assess the functions of brain barriers albumin quotient（QAlb） was used.Expression of IL-1β（Il1B） and its receptor type Ⅰ（Il1r1） and type Ⅱ（Il1r2） and matrix metalloproteinase（Mmp） 3 and 9 was evaluated in the choroid plexus（CP）.Results： Before LPS administration, IL-1β was on the level of 62.0 ± 29.7 pg/mL and 66.4 ± 32.1 pg/mL in plasma and 26.2 ± 5.4 pg/mL and 21.3 ± 8.7 pg/mL in the CSF in sham-and melatonin-implanted group, respectively.Following LPS it increased to 159.3 ± 53.1 pg/mL and 197.8 ± 42.8 pg/mL in plasma and 129.8 ± 54.2 pg/mL and139.6 ± 51.5 pg/mL in the CSF. No correlations was found between plasma and CSF IL-1β concentration after LPS in both groups. The QAlb calculated before LPS and 6 h after was similar in all groups. Melatonin did not affected m RNA expression of Il1B, Il1r1 and Il1r2 in the CP. The m RNA expression of Mmp3 and Mmp9 was not detected.Conclusions： The lack of correlation between plasma and CSF IL-1β concentration indicates that at the beginning of inflammation the local synthesis of IL-1β in the CP is an important source of IL-1β in the CSF. Melatonin from slow-release implants does not affect IL-1β concentration in plasma and CSF in early stage of systemic inflammation.
文摘Objective: To determine if an amniotic fluid (AF)-specific marker is present and if its concentration changes with the presence of labor. Study Design: Twenty-six healthy women who gave birth to healthy newborns at term during the period from July 2009 to January 2010 were included in the study. Six candidate markers were assessed by commercially available ELISA kits: interleukin (IL)-6, squamous cell carcinoma (SCC) antigen, insulin-like growth factor (IGFBP)-1, osteopontin (OPN), CA125, and sialyl Tn (STN). Results: The AF/maternal serum (MS) measurement based on IL-6 or SCC has proved to be superior to IGFBP-1, CA125, OPN and STN. Women with spontaneous labor at term had significantly higher IL-6 and IGFBP-1 concentrations in AF compared with those without labor. No significant differences were observed in the AF concentrations of SCC, OPN, CA125 and STN between women with labor and those not in labor. Conclusion: Our observation of IL-6 and SCC in AF may open a new area of research to assess their usefulness as biological markers of obstetrical disorders.
基金supported by a grant from the National Natural Science Foundation of China,No.30972766,31170852,81001322,81172795,81173048the Specialized Research Fund for the Doctoral Program of Colleges and Universities,No.20094402110004
文摘Interleukin-6 has been shown to be involved in nerve injury and nerve regeneration, but the effects of long-term administration of high concentrations of interleukin-6 on neurons in the central nervous system is poorly understood. This study investigated the effects of 24 hour expo-sure of interleukin-6 on cortical neurons at various concentrations （0.1, 1, 5 and 10 ng/mL） and the effects of 10 ng/mL interleukin-6 exposure to cortical neurons for various durations （2, 4, 8, 24 and 48 hours） by studying voltage-gated Na＋ channels using a patch-clamp technique. Volt-age-clamp recording results demonstrated that interleukin-6 suppressed Na＋ currents through its receptor in a time- and dose-dependent manner, but did not alter voltage-dependent activation and inactivation. Current-clamp recording results were consistent with voltage-clamp recording results. Interleukin-6 reduced the action potential amplitude of cortical neurons, but did not change the action potential threshold. The regulation of voltage-gated Na＋channels in rat corti-cal neurons by interleukin-6 is time- and dose-dependent.
文摘To investigate the levels of cytokines in subeschar edema fluid (SEF). Methods: A guinea pig model with III°30% total body surface area (TBSA) was designed. SEF was collected serially at the time of escharectomy for up to 72 h after scald injury. The levels of tumor necrosis factor.(TNFα), interleukin-6 (IL-6) and interleukin-8 (IL-8) were determined by using the enzyme-linked immunosorbent assay (ELISA). Results: The levels of TNFα,IL-6 and IL-8 in plasma and SEF increased in the early phase of scald injury. Furthermore, the contents of TNFα， IL-6 and IL-8 in SEF were higher than those in plasma.Conclusion: The findings indicate that the subeschar edema fluid is a biologically active reservoir, and is an important participant in the postburn pathophysiologic process.
文摘目的分析外伤性脑损伤（TBI）患者脑脊液及血清Nogo-A基因、白细胞介素1β（IL-1β）及白细胞介素6（IL-6）的表达。方法选取2012年10月~2015年5月江苏大学附属武进医院及江苏省宜兴市人民医院收治的70例TBI患者,按照格拉斯哥昏迷评分（GCS）分将其为轻度组（n=26）、中度组（n=21）及重度组（n=23）,分别于入院第1、4、7、10天采集其脑脊液及静脉血,比较各组患者脑脊液及血清Nogo-A m RNA、Nogo-A蛋白、IL-1β及IL-6表达的差异。结果随着伤情的加重及时间的增加,TBI患者脑脊液及血清中Nogo-A m RNA表达水平、NogoA蛋白浓度、IL-1β、IL-6均呈现上升趋势,伤后第4天达到峰值,并于受伤7 d后逐步下降（P〈0.05）。Pearson相关分析显示,脑脊液和血清中上述因子水平与患者的预后质量呈负相关,且差异有统计学意义（P〈0.05）。结论随着TBI严重程度的增加,患者脑脊液、血清Nogo-A基因、IL-1β、IL-6表达均显著增加,与患者的预后呈负相关,脑脊液内Nogo-A、IL-1β、IL-6表达水平与患者病情变化更为一致。
