目的 :观察内毒素 (L PS)致伤大鼠血浆 IL 10及肺组织 IL 10 m RNA含量和激活蛋白 1(AP 1)活性的变化 ,探讨 AP 1对 IL 10基因表达的作用。方法 :采用 L PS(2、4、6和 8mg/kg)致伤 Wistar大鼠 ,在各时间点 (1、2、4和 6小时 )制备动脉...目的 :观察内毒素 (L PS)致伤大鼠血浆 IL 10及肺组织 IL 10 m RNA含量和激活蛋白 1(AP 1)活性的变化 ,探讨 AP 1对 IL 10基因表达的作用。方法 :采用 L PS(2、4、6和 8mg/kg)致伤 Wistar大鼠 ,在各时间点 (1、2、4和 6小时 )制备动脉血浆并取肺组织。采用酶联免疫吸附法 (EL ISA)检测血浆 IL 10含量 ,采用逆转录聚合酶链反应 (RT PCR)检测 IL 10 m RNA含量 ;采用凝胶迁移率分析法 (EMSA)检测 AP 1活性。结果 :1L PS可使血浆 IL 10含量及肺组织的 IL 10 m RNA含量和 AP 1活性同步升高 ;2 L PS≥6 mg/kg时 ,血浆 IL 10含量及肺组织的 IL 10 m RNA含量和 AP 1活性的高峰增长幅度显著加大。结论 :急性肺损伤或急性呼吸窘迫综合征时 IL 10基因表达增强与 AP 1活性同步升高有关。展开更多
[Objective] This study aimed to obtain IL-10 (interleukin 10) full-length cDNA of common carp (Cyprinus carpio L.) and conduct the sequence analysis. [Method] The differentially expressed cDNA fragment was obtained by...[Objective] This study aimed to obtain IL-10 (interleukin 10) full-length cDNA of common carp (Cyprinus carpio L.) and conduct the sequence analysis. [Method] The differentially expressed cDNA fragment was obtained by DD-RTPCR (differential display RT-PCR). The cDNA library of peripheral blood leukocytes which were separated from common carp and stimulated by mitogen was screened with a probe labeled with DIG (digoxigenin). The IL-10 full-length cDNA was cloned from 0.8×104 pfu of recombinant phages, and the sequence analysis and homology comparison were carried out. [Result] Sequence analysis indicated that the IL-10 full-length cDNA of common carp was 1 117 bp long, containing a 55 bp 5’-UTR, a 522 bp 3’-UTR, and a 540 bp open reading frame(ORF) encoding 179 amino acids. In addition, there were three mRNA instability motifs (ATTTA) in the 3’-untranslated region. The deduced protein sequence shared typical sequence features of the IL-10 family. Homology comparison indicated that the obtained sequence shared 89.1% homology with the carp IL-10 gene from GenBank. [Conclusion] This study laid foundation for further study of the expression manner, functional characteristic and regulation mechanism of IL-10 in vivo and the interaction mechanism in the inflammatory reaction and immune response.展开更多
丙型肝炎病毒（hepatitis C virus,HCV）是导致慢性肝炎的主要病原之一^（[1]）,HCV感染后20%～30%患者能自主清除,近70%可发展为慢性感染^（[2]）。免疫应答过程中分泌的各种细胞因子在病毒性肝炎转归期起重要作用[3],白细胞介素10（in...丙型肝炎病毒（hepatitis C virus,HCV）是导致慢性肝炎的主要病原之一^（[1]）,HCV感染后20%～30%患者能自主清除,近70%可发展为慢性感染^（[2]）。免疫应答过程中分泌的各种细胞因子在病毒性肝炎转归期起重要作用[3],白细胞介素10（interleukin 10,IL-10）和IL-12在宿主防御、免疫稳态中起重要调节作用。展开更多
文摘目的 :观察内毒素 (L PS)致伤大鼠血浆 IL 10及肺组织 IL 10 m RNA含量和激活蛋白 1(AP 1)活性的变化 ,探讨 AP 1对 IL 10基因表达的作用。方法 :采用 L PS(2、4、6和 8mg/kg)致伤 Wistar大鼠 ,在各时间点 (1、2、4和 6小时 )制备动脉血浆并取肺组织。采用酶联免疫吸附法 (EL ISA)检测血浆 IL 10含量 ,采用逆转录聚合酶链反应 (RT PCR)检测 IL 10 m RNA含量 ;采用凝胶迁移率分析法 (EMSA)检测 AP 1活性。结果 :1L PS可使血浆 IL 10含量及肺组织的 IL 10 m RNA含量和 AP 1活性同步升高 ;2 L PS≥6 mg/kg时 ,血浆 IL 10含量及肺组织的 IL 10 m RNA含量和 AP 1活性的高峰增长幅度显著加大。结论 :急性肺损伤或急性呼吸窘迫综合征时 IL 10基因表达增强与 AP 1活性同步升高有关。
文摘[Objective] This study aimed to obtain IL-10 (interleukin 10) full-length cDNA of common carp (Cyprinus carpio L.) and conduct the sequence analysis. [Method] The differentially expressed cDNA fragment was obtained by DD-RTPCR (differential display RT-PCR). The cDNA library of peripheral blood leukocytes which were separated from common carp and stimulated by mitogen was screened with a probe labeled with DIG (digoxigenin). The IL-10 full-length cDNA was cloned from 0.8×104 pfu of recombinant phages, and the sequence analysis and homology comparison were carried out. [Result] Sequence analysis indicated that the IL-10 full-length cDNA of common carp was 1 117 bp long, containing a 55 bp 5’-UTR, a 522 bp 3’-UTR, and a 540 bp open reading frame(ORF) encoding 179 amino acids. In addition, there were three mRNA instability motifs (ATTTA) in the 3’-untranslated region. The deduced protein sequence shared typical sequence features of the IL-10 family. Homology comparison indicated that the obtained sequence shared 89.1% homology with the carp IL-10 gene from GenBank. [Conclusion] This study laid foundation for further study of the expression manner, functional characteristic and regulation mechanism of IL-10 in vivo and the interaction mechanism in the inflammatory reaction and immune response.
文摘丙型肝炎病毒（hepatitis C virus,HCV）是导致慢性肝炎的主要病原之一^（[1]）,HCV感染后20%～30%患者能自主清除,近70%可发展为慢性感染^（[2]）。免疫应答过程中分泌的各种细胞因子在病毒性肝炎转归期起重要作用[3],白细胞介素10（interleukin 10,IL-10）和IL-12在宿主防御、免疫稳态中起重要调节作用。
