Objective To analyze the blastocyst formation and chromosome statuses of reconstructed embryos derived from human-goat interspecies somatic cell nuclear transfer （iSCNT）, exploring the development retardant factors....Objective To analyze the blastocyst formation and chromosome statuses of reconstructed embryos derived from human-goat interspecies somatic cell nuclear transfer （iSCNT）, exploring the development retardant factors. Methods Human specific point probes cep2, cep6, tel2 and 13q14.2, 21q22.13 combining fluorescence in-situ hybridization （FISH） technology were used to test trophectoderm cells of blastocyst and blastomeres of development arrest nuclear transfer （NT） embryos. Results A total of 209 reconstructed embryos were recovered, and the rate of blastocyst formation was 3.8% （8/209）. FISH signals showed that chromosomal abnormalities were present in 2 blastocysts （2/8） and 146 development arrest embryos （146/201）. Conclusion The rate of blastocyst formation is low, and reconstituted embryos of development arrest showed extensive chromosome abnormalities, suggesting that a chromosomal mechanism may underlie their developmental arrest.展开更多
reconstituted embryos were produced by nuclear transplantation using bovine ear fibroblasts at G 0 or non-G 0 stage as donor nuclei and oocytes collected from superovulated multiparous or young rabbits as recipi...reconstituted embryos were produced by nuclear transplantation using bovine ear fibroblasts at G 0 or non-G 0 stage as donor nuclei and oocytes collected from superovulated multiparous or young rabbits as recipients. After cultivation in two kinds of medium M199+10%FBS or RD+10%FBS, 112 of them developed to 2-cell stage (62.2%) and 26 to morula stage (14.4%) and 20 of them eventually developed to blastocyst stage (11.1%). There is no significant difference for the cleavage rates in two groups of reconstituted embryos derived from G 0 -stage and non-G 0 stage donor cells respectively. However, G 0-stage donor cells could result in higher rate of 8-cell16-cell stage embryos significantly (P<0.05), as well as higher rate of blastocysts (P<0.01). It seems that using two different culture systems had no significant effects on the cleavage rate, morula rate or blastocyst rate (P>0.05).展开更多
基金supported by Grants from Special Fund for Excellent Young University teachers in Shanghai 2012Shanghai Science and Technology Developmental Foundations (Grant number: 09ZR1419000)
文摘Objective To analyze the blastocyst formation and chromosome statuses of reconstructed embryos derived from human-goat interspecies somatic cell nuclear transfer （iSCNT）, exploring the development retardant factors. Methods Human specific point probes cep2, cep6, tel2 and 13q14.2, 21q22.13 combining fluorescence in-situ hybridization （FISH） technology were used to test trophectoderm cells of blastocyst and blastomeres of development arrest nuclear transfer （NT） embryos. Results A total of 209 reconstructed embryos were recovered, and the rate of blastocyst formation was 3.8% （8/209）. FISH signals showed that chromosomal abnormalities were present in 2 blastocysts （2/8） and 146 development arrest embryos （146/201）. Conclusion The rate of blastocyst formation is low, and reconstituted embryos of development arrest showed extensive chromosome abnormalities, suggesting that a chromosomal mechanism may underlie their developmental arrest.
基金The research was supported by a Key Project of the“Tenth Five-Year”Science&Technology Development Plan(01606006)from Natural Science Foundation of Anhui Province,China.
文摘reconstituted embryos were produced by nuclear transplantation using bovine ear fibroblasts at G 0 or non-G 0 stage as donor nuclei and oocytes collected from superovulated multiparous or young rabbits as recipients. After cultivation in two kinds of medium M199+10%FBS or RD+10%FBS, 112 of them developed to 2-cell stage (62.2%) and 26 to morula stage (14.4%) and 20 of them eventually developed to blastocyst stage (11.1%). There is no significant difference for the cleavage rates in two groups of reconstituted embryos derived from G 0 -stage and non-G 0 stage donor cells respectively. However, G 0-stage donor cells could result in higher rate of 8-cell16-cell stage embryos significantly (P<0.05), as well as higher rate of blastocysts (P<0.01). It seems that using two different culture systems had no significant effects on the cleavage rate, morula rate or blastocyst rate (P>0.05).
