Background and Objectives: Increased expression of the CD97, nuclear factor-kB (NF-kB) and cyclooxygenase-2 (COX-2) has been found to play an important role in development of many cancers, including gastric neoplasm. ...Background and Objectives: Increased expression of the CD97, nuclear factor-kB (NF-kB) and cyclooxygenase-2 (COX-2) has been found to play an important role in development of many cancers, including gastric neoplasm. However, the expression and biological behavior of CD97, NF-kB and COX-2 in gastric MALT (mucosa-associated lymphoid tissue) lymphoma has not been well investigated. Methods: The expressions of CD97, COX-2 and NF-kB in 47 cases of gastric MALT lymphoma were detected immunohistochemically, and the relevance between their expressions and the biological behavior was analyzed retrospectively. Results: 1) The expressions of CD97, NF-kB and COX-2 were 87.2%, 36.2% and 48.9%, respectively;2) The difference of CD97 expression between depth of invasion limited in mucosa and submucosa and beyond muscularis propria was significant (100.0% vs. 71.4%, P < 0.01). Moreover, the expression of nuclear CD97 between stage IIE, III, IV and stage I patients showed significant difference (96.4% vs. 73.7%, P < 0.05);3) The expression of NF-kB was significantly correlated with tumor size, depth of invasion and stage;4) The expression of COX-2 was significantly correlated with Helicobacter pylori infection, clinical stage, depth of invasion and tumor size (P < 0.05). Conclusions: Expressions of CD97, NF-κB and COX-2 were correlated with tumor invasion and metastasis in gastric MALT lymphoma.展开更多
目的建立兔颈总动脉-颈内静脉内瘘模型;探讨血管外膜涂层缓释西罗莫司对内膜增生的影响。方法健康雄性新西兰大耳白兔18只,随机分为对照组、F-127组和F-127+西罗莫司组,每组6只,将兔颈总动脉与颈内静脉端吻合,建立内瘘模型后,按组别在...目的建立兔颈总动脉-颈内静脉内瘘模型;探讨血管外膜涂层缓释西罗莫司对内膜增生的影响。方法健康雄性新西兰大耳白兔18只,随机分为对照组、F-127组和F-127+西罗莫司组,每组6只,将兔颈总动脉与颈内静脉端吻合,建立内瘘模型后,按组别在吻合口进行不同处理:对照组,不给予任何处理;F-127组,涂抹20%pluronic F-127多聚凝胶0.5 m L;F-127+西罗莫司组,涂抹携带西罗莫司0.5 mg的20%pluronic F-127多聚凝胶0.5 m L。术后3周,获取近吻合口处静脉血管,采用苏木精-伊红染色法观察静脉内膜增生情况,测量静脉血管内膜厚度。采用免疫组织化学法及Western blotting法检测各组转化生长因子β1(TGF-β1)和结缔组织生长因子(CTGF)的表达。结果术后3周,对照组和F-127组较F-127+西罗莫司组血管内膜增生明显(P<0.05);对照组和F-127组较F-127+西罗莫司组TGF-β1和CTGF表达明显升高(P<0.05),对照组与F-127组之间差异无统计学意义(P>0.05)。结论西罗莫司血管外膜涂层缓释可有效抑制内瘘血管内膜的增生;西罗莫司可通过抑制TGF-β1和CTGF的表达来抑制血管内膜纤维化过程。展开更多
文摘Background and Objectives: Increased expression of the CD97, nuclear factor-kB (NF-kB) and cyclooxygenase-2 (COX-2) has been found to play an important role in development of many cancers, including gastric neoplasm. However, the expression and biological behavior of CD97, NF-kB and COX-2 in gastric MALT (mucosa-associated lymphoid tissue) lymphoma has not been well investigated. Methods: The expressions of CD97, COX-2 and NF-kB in 47 cases of gastric MALT lymphoma were detected immunohistochemically, and the relevance between their expressions and the biological behavior was analyzed retrospectively. Results: 1) The expressions of CD97, NF-kB and COX-2 were 87.2%, 36.2% and 48.9%, respectively;2) The difference of CD97 expression between depth of invasion limited in mucosa and submucosa and beyond muscularis propria was significant (100.0% vs. 71.4%, P < 0.01). Moreover, the expression of nuclear CD97 between stage IIE, III, IV and stage I patients showed significant difference (96.4% vs. 73.7%, P < 0.05);3) The expression of NF-kB was significantly correlated with tumor size, depth of invasion and stage;4) The expression of COX-2 was significantly correlated with Helicobacter pylori infection, clinical stage, depth of invasion and tumor size (P < 0.05). Conclusions: Expressions of CD97, NF-κB and COX-2 were correlated with tumor invasion and metastasis in gastric MALT lymphoma.
文摘目的建立兔颈总动脉-颈内静脉内瘘模型;探讨血管外膜涂层缓释西罗莫司对内膜增生的影响。方法健康雄性新西兰大耳白兔18只,随机分为对照组、F-127组和F-127+西罗莫司组,每组6只,将兔颈总动脉与颈内静脉端吻合,建立内瘘模型后,按组别在吻合口进行不同处理:对照组,不给予任何处理;F-127组,涂抹20%pluronic F-127多聚凝胶0.5 m L;F-127+西罗莫司组,涂抹携带西罗莫司0.5 mg的20%pluronic F-127多聚凝胶0.5 m L。术后3周,获取近吻合口处静脉血管,采用苏木精-伊红染色法观察静脉内膜增生情况,测量静脉血管内膜厚度。采用免疫组织化学法及Western blotting法检测各组转化生长因子β1(TGF-β1)和结缔组织生长因子(CTGF)的表达。结果术后3周,对照组和F-127组较F-127+西罗莫司组血管内膜增生明显(P<0.05);对照组和F-127组较F-127+西罗莫司组TGF-β1和CTGF表达明显升高(P<0.05),对照组与F-127组之间差异无统计学意义(P>0.05)。结论西罗莫司血管外膜涂层缓释可有效抑制内瘘血管内膜的增生;西罗莫司可通过抑制TGF-β1和CTGF的表达来抑制血管内膜纤维化过程。
