Num an Reproductive Health MedicalCenter # Departm entof Physiology Corresponding author: YEYang Fax: 010-62179119; E-m ail: yey@ m ail. Com NationalResearch Institute for Fam ily Planning, Beijing 100081, China...Num an Reproductive Health MedicalCenter # Departm entof Physiology Corresponding author: YEYang Fax: 010-62179119; E-m ail: yey@ m ail. Com NationalResearch Institute for Fam ily Planning, Beijing 100081, China In thepresentstudy, m aleSD ratsw ereused to evaluatetheefficacy of a new vas occlusion device, intra-vasdevice(IVD) and long-term m orphologicalchange of both vasand IVD. After inserting IVD into bilateralvas, allrats (9) becam e sterilized as proved by m atetestand thefactthatno sperm w asfound in fem alevagina afterm at- ing. Half a year later, there had been a large am ountof sperm in vaslum en dilated and sperm granulom a w asfound in theproxim alend of IVD. Scanning electron m i- croscope studiesshow ed thatnylon threadsin IVD w ere covered by protein likem atter. There w ere som e norm al sperm s betw een the nylon sutures. A few sperm s w ith de- form ed structurew erefound in thedistalend of IVD. Conclusion: our studiesshow ed that IVD could be an effective device in SD rats. Fluid and som e deform ed sperm s m ay pass through IVD, but these sperm s have no fertility. The m echanism of IVD needsto be further investigated.展开更多
The Context: Aims: To evaluate the biocompatibility and in vitro genotoxicity of a non-copper nano silica polymer modified composite for filtering-type intra-vas devices. Settings and Design: Academic research laborat...The Context: Aims: To evaluate the biocompatibility and in vitro genotoxicity of a non-copper nano silica polymer modified composite for filtering-type intra-vas devices. Settings and Design: Academic research laboratory, Huazhong University of Science and Technology. Prospective experimental study. Methods and Material: Non-copper nano silica polymer modified composite rods were implanted into the back muscle of rabbits for biocompatibility evaluation. Comet assay was applied to the determination of DNA damage, while, Mutagenic activity was tested by means of Ames test using Salmonella typhimurium TA98 and TA 100 tester strains with and without metabolic activation. Statistical analysis used: qualitative and quantitative data were tested using the Chi-square test and Student’s test. Results: Only mild inflammatory reaction was observed in the surrounding tissues of the implanted nano-silica modified polymer composite in the early implantation stage, which was similar to that of the sham-operated group. The inflammatory reaction was completely disappeared after 12 weeks. No significant DNA damage (P > 0.05) were tested on the nano-silica modified polymer composite in Comet assay. In Ames test, the extracts from non-copper composite did not exert mutagenic effect on the bacterial. Conclusions: The non-copper nano silica modified composite did not exhibit in vitro genotoxicity and obvious inflammation in tissue, it would be a safe biomaterial for further clinical trial.展开更多
文摘Num an Reproductive Health MedicalCenter # Departm entof Physiology Corresponding author: YEYang Fax: 010-62179119; E-m ail: yey@ m ail. Com NationalResearch Institute for Fam ily Planning, Beijing 100081, China In thepresentstudy, m aleSD ratsw ereused to evaluatetheefficacy of a new vas occlusion device, intra-vasdevice(IVD) and long-term m orphologicalchange of both vasand IVD. After inserting IVD into bilateralvas, allrats (9) becam e sterilized as proved by m atetestand thefactthatno sperm w asfound in fem alevagina afterm at- ing. Half a year later, there had been a large am ountof sperm in vaslum en dilated and sperm granulom a w asfound in theproxim alend of IVD. Scanning electron m i- croscope studiesshow ed thatnylon threadsin IVD w ere covered by protein likem atter. There w ere som e norm al sperm s betw een the nylon sutures. A few sperm s w ith de- form ed structurew erefound in thedistalend of IVD. Conclusion: our studiesshow ed that IVD could be an effective device in SD rats. Fluid and som e deform ed sperm s m ay pass through IVD, but these sperm s have no fertility. The m echanism of IVD needsto be further investigated.
文摘The Context: Aims: To evaluate the biocompatibility and in vitro genotoxicity of a non-copper nano silica polymer modified composite for filtering-type intra-vas devices. Settings and Design: Academic research laboratory, Huazhong University of Science and Technology. Prospective experimental study. Methods and Material: Non-copper nano silica polymer modified composite rods were implanted into the back muscle of rabbits for biocompatibility evaluation. Comet assay was applied to the determination of DNA damage, while, Mutagenic activity was tested by means of Ames test using Salmonella typhimurium TA98 and TA 100 tester strains with and without metabolic activation. Statistical analysis used: qualitative and quantitative data were tested using the Chi-square test and Student’s test. Results: Only mild inflammatory reaction was observed in the surrounding tissues of the implanted nano-silica modified polymer composite in the early implantation stage, which was similar to that of the sham-operated group. The inflammatory reaction was completely disappeared after 12 weeks. No significant DNA damage (P > 0.05) were tested on the nano-silica modified polymer composite in Comet assay. In Ames test, the extracts from non-copper composite did not exert mutagenic effect on the bacterial. Conclusions: The non-copper nano silica modified composite did not exhibit in vitro genotoxicity and obvious inflammation in tissue, it would be a safe biomaterial for further clinical trial.
