Cytosolic protein delivery techniques are of great importance for cell biology,biotechnology and protein drug development.The design of carriers with robust efficiency in cytosolic protein delivery is challenging.This...Cytosolic protein delivery techniques are of great importance for cell biology,biotechnology and protein drug development.The design of carriers with robust efficiency in cytosolic protein delivery is challenging.This account provides a progress report of polymeric carriers for this purpose in our group.During the past years,we have developed several types of functionalized polymers for cytosolic protein and peptide delivery by engineering polymers with ligands such as guanidinium,boronate,coordination ligands and fluoroalkyls.The designed polymers showed improved protein/peptide binding affinities,and successfully delivered various cargo proteins into the cytosol of living cells,while maintaining their bioactivity.In addition,the polymers showed potent efficiencies in the delivery of tumor antigens,therapeutic peptides,toxins and antioxidant proteins in vivo.We hope these polymers could be translated for protein delivery in the treatment of various diseases in the future.展开更多
基金the National Key R&D Program of China,Synthetic Biology Research(No.2019YFA0904500)the National Natural Science Foundation of China(No.21725402)the Guangdong Provincial Key Laboratory of Functional and Intelligent Hybrid Materials and Devices(No.2019B121203003).
文摘Cytosolic protein delivery techniques are of great importance for cell biology,biotechnology and protein drug development.The design of carriers with robust efficiency in cytosolic protein delivery is challenging.This account provides a progress report of polymeric carriers for this purpose in our group.During the past years,we have developed several types of functionalized polymers for cytosolic protein and peptide delivery by engineering polymers with ligands such as guanidinium,boronate,coordination ligands and fluoroalkyls.The designed polymers showed improved protein/peptide binding affinities,and successfully delivered various cargo proteins into the cytosol of living cells,while maintaining their bioactivity.In addition,the polymers showed potent efficiencies in the delivery of tumor antigens,therapeutic peptides,toxins and antioxidant proteins in vivo.We hope these polymers could be translated for protein delivery in the treatment of various diseases in the future.
