Herbal Textual Research of Inulae Flos in Chinese Classic Prescription

The herbal textual research of Inulae Flos was carried out from the aspects of name,origin,habit and distribution,nature and flavor,meridian,efficacy and indications by referring to ancient Chinese herbal books and different editions of Pharmacopoeia of People’s Republic of China.After textual research,Inulae Flos has been named as"Inulae Flos"since Shennong Bencaojing(Shennong’s Herbal Classic).Its original plant is Inula japonica Thunb.or I.britannica L.,which is easy to multiply and widely distributed.Inulae Flos is used medically with clean and dry capitulum,and there is also honey-burning method.The description of Inulae Flos in Pharmacopoeia of People’s Republic of China,such as nature and flavor,meridian,efficacy and indications,is generally consistent with the records in traditional herbal books.

金银花茎的浸提液对旋覆花的化感作用

为了研究金银花茎的浸提液对旋覆花幼苗的化感作用及利用化感作用进行生物防除的可能性,论文采用水提法提取金银花茎的浸提液.试验设置0.02、0.04、0.06、0.08 g/mL四个质量浓度梯度,并以蒸馏水为对照组(CK),通过测定旋覆花幼苗的形态与生理指标,研究金银花茎的浸提液对旋覆花的化感效应.结果表明,旋覆花幼苗在金银花茎浸提液的作用下,除旋覆花叶片中的含丙二醛量有所升高外,旋覆花的苗高、根长、苗的干质量以及叶片中过氧化物酶活性等都受到抑制,且随着浸提液质量浓度的升高,抑制作用增强.当质量浓度为0.08 g/mL时,苗高、根长、苗的干生物量及过氧化物酶活性与对照相比分别降低了11.95%、24.95%、31.25%、30.90%.由此可得,金银花茎的浸提液对旋覆花幼苗生长有明显的化感抑制作用,因此其对一定区域的旋覆花幼苗的防除是可行的.

基于UPLC-Q-Orbitrap HRMS及GC-MS结合网络药理学和分子对接的彝药黑根抗类风湿性关节炎的药效物质研究

目的:探讨彝药黑根治疗类风湿性关节炎潜在的作用机制以及主要活性成分。方法:运用UPLC-Q-Orbitrap HRMS及GC-MS技术分析黑根的化学成分,利用Swiss Target进行活性成分的靶点筛选和预测,同时在Genecard、OMIM、Dis Ge NET等数据库中筛选类风湿性关节炎相关靶点,利用Cytoscape3.9.0软件构建“活性成分-靶点”网络模型,运用String数据分析平台进行蛋白互作网络分析,并且利用基因富集分析在线工具对核心靶点进行GO富集分析和KEGG通路分析,最后使用分子对接对网络药理学内容进行初步验证。结果:共鉴定出黑根化学成分78个,筛选后活性成分50个,237个潜在作用靶点与类风湿性关节炎相关,包括STAT3、AKT1、EGFR等关键靶点。通路富集分析PI3K-Akt通路、JAK-STAT等信号通路可能是黑根发挥抗类风湿性关节炎的主要途径。通过分子对接技术得出主要活性成分(异绿原酸B、异绿原酸C)与关键靶点有较好结合力。结论:研究初步表明黑根通过多成分、多靶点、多途径治疗类风湿性关节炎的作用,为后续黑根物质基础研究提供了理论依据。

沙旋覆花石油醚部位洗脱组分的体外抗肿瘤活性研究

采用CCK-8法从沙旋覆花中筛选出具有抗肿瘤活性的石油醚部位洗脱组分,研究了其对人食管癌细胞Eca109增殖的抑制作用,并通过4′,6-二脒基-2-苯基吲哚(DAPI)染色法探究了其对Eca109细胞抑制作用的方式。结果表明,沙旋覆花石油醚部位洗脱组分(除23^(#)外)对Eca109细胞具有不同程度的抑制作用,其中71^(#)组分的抑制作用最强,对Eca109细胞的IC_(50)值为8.19μg·mL^(-1);沙旋覆花石油醚部位洗脱组分可通过诱导细胞裂亡和凋亡发挥抗食管癌作用,为后续开展旋覆花属植物抗食管癌活性成分筛选与药效评价奠定了基础。

基于抗氧化活性筛选旋覆花提取工艺及干燥技术的研究

目的以单因素和效应面法(Box-Behnken)结合探究旋覆花提取工艺,并评价不同干燥方式对提取物抗氧化能力的影响。方法以麝香草酚含量和浸膏得率为指标,在单因素的基础上,考察料液比、提取时间、提取次数对提取工艺的影响。通过测定提取物对DPPH自由基清除率,比较5种不同干燥方式的影响。结果最佳提取工艺为料液比为1∶30,提取时间为40 min,提取1次;重复工艺所得麝香草酚含量和浸膏得率符合预测值;真空冷冻干燥对DPPH自由基清除率最高。结论所得参数可作为旋覆花提取物的制备工艺参数,并为开发旋覆花抗氧化产品生产时的干燥工艺提供科学依据。

旋覆代赭汤加味治疗脾胃虚弱型2型糖尿病胃轻瘫的护理配合及效果研究

目的 探讨旋覆代赭汤加味治疗脾胃虚弱型2型糖尿病胃轻瘫的护理配合及效果。方法 选取2020年1月—2021年12月在本院治疗的脾胃虚弱型2型糖尿病胃轻瘫患者29例为研究对象,按照随机数字表法分为观察组(n=15)和对照组(n=14)。两组患者均给予口服多潘立酮片联合旋覆代赭汤加味治疗,同时对照组患者予以常规护理方法,观察组患者在常规护理的基础上实施个体化护理方法。比较两组患者干预前后胃肠激素[胃动素(MTL)、胃泌素(GAS)以及生长抑素(SS)]、消化道症状评分。结果 干预后,两组MTL、GAS、SS水平均明显降低,与干预前比较差异有统计学意义(P<0.05),且观察组MTL、GAS、SS水平降低幅度大于对照组,差异有统计学意义(P<0.05);两组患者腹胀、厌食、早饱、腹痛、恶心或呕吐、嗳气症状评分均明显降低,与治疗前比较差异有统计学意义(P<0.05),且观察组患者腹胀、厌食、早饱、腹痛、恶心或呕吐、嗳气症状评分降低幅度大于对照组,差异有统计学意义(P<0.05)。结论 对旋覆代赭汤加味治疗脾胃虚弱型2型糖尿病胃轻瘫的患者实施个体化护理干预有利于改善临床症状,调节胃肠激素水平,值得临床应用推广。

旋覆花药性研究古今演变及原因探析

考证旋覆花四气、五味、归经、升降浮沉等药性的演变脉络,初步探析已有记载中对旋覆花药性认识不一的成因。《神农本草经》和《名医别录》奠定了旋覆花药性理论的基础,历代本草文献以此为主体进行了扩充。隋唐以后,旋覆花的临床应用范围扩大,将其用于治疗头面部疾病。宋代打破了长期以来对旋覆花“性专主下”的认识,开始从味苦辛、性走散的角度阐释其药性特点。至明清时期,出现了“性专主下”“能升能降”“药性旋转”的多元化认识。古代医籍中所载旋覆花药物品种有差异、药用部位不统一、临床配伍变化较丰富,上述因素均有可能对药性的判定产生影响。

Two New Sesquiterpenes from Inula japonica

Two new sesquiterpenes were isolated from the aerial parts of Inula japonica. Their structures were elucidated as 1-hydroxy-8-acetoxycostic acid methyl ester and 1-hydroxy-8-acetoxyisocostic acid methyl ester by spectral methods.

Hepato-protective Effect of Polysaccharides Extracted from Inula Britannica Flower for Mice

The polysaccharides extracted from Inula britannica flower（IBP） exhibited a significant anti-liver injury activity.The properties and chemical compositions of IBP were analyzed via high performance liquid chromatography（HPLC） and infrared spectroscopy（IR） methods.The results show that the molecular weight distribution of IBP is from 600 to 3500.The weight-average molecular weight is 1099.IBP consists of mannose,glucuronic acid,rhamnose,galacturonic acid,glucose,galactose and arabinose with a molar ratio of 4.1：1：1.4：2.7：14.6：6.3：7.9.The IR spectrum of IBP reveals the typical characteristics of polysaccharides and proteins.After administration of IBP with 100,50 and 25 mg/kg body mass to liver injury mice induced by CCl 4 or bacillus calmette guerin-lipopolysaccharide（BCG-LPS）,not only the blood alanine aminotransferase（ALT） and aspartate aminotransferase（AST） levels decreased significantly（P0.05）,but aslo the liver tissue malondialdehyde（MDA） level decreased significantly（P0.01）.The superoxide dismutase（SOD） level increased significantly（P0.01）.IBP shows significant anti-liver injury and antioxidation activity.

Anthranilic acid derivatives from Inula japonica

Three new anthranilic acid derivatives, N-heneicosanoylanthranilic acid （1b）, N-tricosanoylanthranilic acid （1d）, N-tetracosanoylanthranilic acid （1e）, and two known N-arachidylanthranilic acid （1a） and N-docosanoylanthranilic acid （1c） were isolated from the aerial parts of lnula japonica Thunb. Their structures were established by spectroscopic and chemical methods.

Antioxidant activities and total phenol content of Inula viscosa extracts selected from three regions of Morocco

Objective:To investigate antioxidant activity,total phenolic and flavonoid content of ethanol(E) and ethyl acetate(A) extracts of Inula viscosa aerial parts selected from three regions of Morocco(Imouzzer.Sefrou and Taounate).Methods:Antioxidants properties were measured by three different test systems of assay namely free radical scavenging activities against 2,2-diphenyI-1-picrylhydrazyl,total antioxidant capacity and iron-reducing capacity.Total phenolic content was measured by FolinCiocalteu reagent.Results:All the extracts showed significant antioxidant activities and contained important levels of phenols.The ethanol extract(0.3 mg/mL) from Sefrou showed the greatest antioxidant capacity in the three systems of assay,which was probably due to its high content of polyphenols(274.39±6.94) mg gallic acid equivalent/g dry extract.Total flavonoid content was found equal for all extracts.Conclusions:Our results of antioxidant assays were justified and partially supported the popular usage of the tested plants.The high antioxidant activity found in the plant from Sefrou and its great biomass in this region suggested that Inula viscosa is a good source of natural antioxidants compounds which might have benefits for health.

Essential Oil from Inula britannica Extraction with SF-CO_2 and Its Antifungal Activity

The aim of this study was to determine the extraction technique of supercritical fluid carbon dioxide（SF-CO 2） for the essential oil from Inula britannica flowers and its antifungal activities against plant pathogenic fungi for its potential application as botanical fungicide.The effects of factors,including extraction temperature,extraction pressure,SF-CO 2 flow rate,flower powder size,and time on the essential oil yield were studied using the single factor experiment.An orthogonal experiment was conducted to determine the best operating conditions for the maximum extraction oil yield.Adopting the optimum conditions,the maximum yield reached 10.01% at 40°C temperature,30 MPa pressure,60 mesh flower powder size,20 L h-1SF-CO 2 flow rate,and 90 min extraction time.The antifungal activities of I.britannica essential oil using the SF-CO 2 against the most important plant pathogenic fungi were also examined through in vitro and in vivo tests.Sixteen plant pathogenic fungi were inhibited to varying degrees at 1 mg mL-1concentration of the essential oil.The mycelial growth of Gaeumannomyces graminis var.tritici was completely inhibited.The radial growths of Phytophthora capsici and Fusarium monilifome were also inhibited by 83.76 and 64.69%,respectively.In addition,the essential oil can inhibit the spore germination of Fusarium oxysporum f.sp.vasinfectum,Phytophthora capsici,Colletotrichum orbiculare,and Pyricularia grisea,and the corresponding inhibition rates were 98.26,96.54,87.89,and 87.35% respectively.The present study has demonstrated that the essential oil of I.britannica flowers extracted through the SF-CO 2 technique is one potential and promising antifungal agent that can be used as botanical fungicide to protect crops.

Geobotanical and Ecological-Physiological Characteristics of Some Medicinal Species of the Genus <i>Inula</i>L. under Conditions of Uzbekistan

The article presents the geobotanical and some bioecological features of species of the genus Inula L., family Asteraceae (Compositae). The water regime, as well as the water holding capacity, transpiration rate and adaptive ability of plants to drought in the arid zone, is considered. The results of studies on the introduction into the culture based on indicators of the osmotic pressure of cell juice are presented.

Effect of Clammy Inula (<i>Inula viscose</i>) Plant Extract in Combination with a Low Dose of the Fungicide Iprodione on <i>Botrytis cinerea in Vitro</i>and <i>in Vivo</i>

The effect of native medical plant “Clammy Inula” (Inula viscosa L.) in combination with a low dose of the fungicide iprodione (Rovral&reg) against gray mould disease (Botryis cinerea Pers.) was evaluated in vitro and in vivo. The results revealed that the plant extract had antifungal effect on mycelial growth rate and conidial germiation of pathogen isolates. The plant extract at the concentration of 1% - 4% and the fungicide iprodione at the concentration of 0.2 - 9 μg&middotml-1 significantly reduced the mycelium growth and germination. In addition, the plant extract at the concentration of 2% - 4% and iprodione at the concentration of 300 - 600 μg&middotml-1 significantly reduced the disease severity (%) on bean plants compared to the controls. The reduction of mycelium growth, germination, and disease severity was positively correlated with increasing plant extract and fungicide concentrations. The combination of a low dose of plant extract and fungicide at (EC50) was able to reduce disease severity of gray mold by 84%. While, plant extract and fungicide alone reduced disease severity by 46% & 39% and by 70% & 76% for the isolate (Bo5-10 and Bc 99), respectively, compared with controls. The application of water extracts of I. viscose in combination of a low dose of an effective fungicide (iprodione) can be a feasible tool in reducing gray mould disease severity, but farther studies are still needed under field conditions to evaluate efficacy against the disease under field conditions.

Some pharmacological studies on the methanolic extract of <i>Inula graveolense</i>L.

Inula graveolens L. is widely used in Iraq for the treatment of rheumatic fever, infant convulsions, toothache, blood sugar, and also to dissolve internal blood clots, and to aid digestion. However, the efficacy and mechanisms of action of the plant remain unclear. Therefore, the objective of the present study was to investigate the pharmacological effects of the methanolic extract (MEIG) of this plant belonging to compositae family. Anti-diarrheal and antipyretic activities of the extract were examined in rats. Anti-inflammatory and antinociceptive were studied in mice. At the doses of 200 (P in vitro protein anti-denaturation using Bovine serum albumin and anti-platelet aggregation of human blood activity. It was observed that the extract showed greater percentage of inhibition of BSA (P potential platelet aggregation inhibitory activity in adose-dependent manner. The maximum inhibition was observed at the dose 400 μg/ml

旋覆花不同部位代谢组比较分析

目的:探索旋覆花Inula japonica Thunb.次生代谢产物成分及其在旋覆花不同部位分布的差异性。方法:采用非靶向代谢组学方法对旋覆花根、茎、叶、花的代谢组进行比较分析。结果:共鉴定出675个代谢物,通过多元统计分析筛选出350个差异代谢物。其中,在23个黄酮类化合物中筛选出15个显著差异代谢物。代谢途径分析表明,淀粉和蔗糖代谢,丙氨酸、天门冬氨酸和谷氨酸代谢,植物激素信号转导代谢途径显著富集。结论:旋覆花不同组织中代谢物分布不同,不同组织间差异代谢物重要代谢途径的解析为旋覆花的合成生物研究提供参考。

旋覆代赭汤加味治疗胃食管反流性咳嗽临床研究

目的:观察旋覆代赭汤加味治疗胃气上逆型胃食管反流性咳嗽(gastroesophageal reflux cough,GERC)的临床疗效。方法:将82例胃气上逆型GERC患者按照随机数字表法分为对照组和研究组,每组各41例。对照组给予奥美拉唑镁肠溶片及枸橼酸莫沙必利片治疗,研究组在对照组治疗的基础上给予旋覆代赭汤加味治疗。比较两组患者的临床疗效及治疗前后中医证候积分、食管运动指标(食管上括约肌静息压、湿咽成功率、食管下括约肌静息压)、胃肠激素水平[血管活性肠肽(vasoactive intestinal peptide,VIP)、胃动素(motilin,MTL)、胃泌素(gastrin,GAS)]、生活质量评分变化情况。结果:研究组有效率为90.24%,对照组有效率为73.17%,两组患者有效率比较,差异具有统计学意义(P<0.05)。两组患者治疗后中医证候积分低于本组治疗前,且治疗后研究组低于对照组,差异具有统计学意义(P<0.05)。两组患者治疗后日间、夜间咳嗽症状积分低于本组治疗前,且治疗后研究组低于对照组,差异具有统计学意义(P<0.05)。两组患者治疗后食管上括约肌静息压、湿咽成功率、食管下括约肌静息压高于本组治疗前,且治疗后研究组高于对照组,差异具有统计学意义(P<0.05)。两组患者治疗后VIP低于本组治疗前,MTL、GAS高于本组治疗前,且治疗后组间比较,差异具有统计学意义(P<0.05)。两组患者治疗后社会功能、心理健康、生理情况等生活质量评分高于本组治疗前,且治疗后研究组高于对照组,差异具有统计学意义(P<0.05)。结论:旋覆代赭汤加味治疗胃气上逆型胃食管反流性咳嗽疗效确切,可显著缓解患者的临床症状,改善食管运动指标及胃肠激素水平。

旋覆花古今功效浅析

旋覆花属植物种类较多,市场来源广泛,加之药用历史悠久,其别名较多,易造成药材混淆。历代文献对于旋覆花的记载和论述较为丰富,历代医家用其降逆之性较多,而疏于其升散作用。该文通过查阅历代医籍及现代文献,从旋覆花别名及药用部位、性味归经、本草功用、方剂学应用、煎煮方法、药理作用等方面进行阐述,以期对旋覆花进一步深入研究提供理论参考,挖掘其内在潜力,丰富临床用药依据。

蒙药土木香的化学成分研究

研究蒙药土木香的化学成分,采用RP-HPLC、硝酸银硅胶色谱等方法分离纯化,应用NMR、MS等波谱技术鉴定化合物结构,共分离鉴定了22个单体化合物,分别为5α,6α-dihydroxy-eudesma-11(13)-dien-12,8β-olide(1)、4α-hydroxy-5-methoxy-5,8α-dimethyl-3-methylenedecahydronaphtho[2,3-b]furan-2-one(2)、木香烯内酯(3)、土木香内酯(4)、异土木香内酯(5)、11,13-二氢土木香内酯(6)、alloalantolactone(7)、4α,5β-环氧-1(10),11(13)-吉玛烷-12,8α-交酯(8)、septuplinolide(9)、8-epi-ivangustin(10)、santamarine(11)、racemosalactone A(12)、3-oxo-eudesma-4(11)-dien-12,8β-olide(13)、telekin(14)、igalane(15)、macrophyllilactone E(16)、4α,15-环氧异土木香内酯(17)、11,13-dihydroxy-alantolactone(18)、1(2),4(15),11(3)-eudesma-trien-12,8β-olide(19)、亚麻酸(20)、亚油酸(21)、油酸(22)。化合物1是新倍半萜内酯类化合物,化合物2、3、9~14、17、19~22为首次从该属植物中分离得到,化合物4~8、15、16、18为土木香药材中已报道的化合物。

重构本草——旋覆花

通过古籍文献调研、中药现代研究进展及其研究成果的梳理,结合中医临床及中药学等学科领域内专家学者的认识及应用经验,经讨论认为旋覆花:主要功效为降气、消痰、行水、止呕,症靶为咳嗽和呕吐。现代药理研究发现,旋覆花及其有效成分具有抗炎、抑制肿瘤增殖、降低气道高反应性、抑制肺纤维化、降低血脂等作用;本品无毒,但是阴虚痨嗽,津伤燥咳者忌服;临床使用剂量为1.5~30 g,内服时当根据不同疾病,或同一疾病的不同阶段进行辨证使用,并积极探索旋覆花用量与症靶的量效关系构建。