Annexin A2 silencing inhibits invasion, migration, and tumorigenic potential of hepatoma cells

AIM: To investigate the effects of Annexin A2 (ANXA2) silencing on invasion, migration, and tumorigenic potential of hepatoma cells. METHODS: Human hepatoma cell lines [HepG2, SMMC-7721, SMMC-7402, and MHCC97-H, a novel human hepatocellular carcinoma (HCC) cell line with high metastasis potential] and a normal hepatocyte cell line(LO2) were used in this study. The protein and mRNA expression levels of ANXA2 were analysed by western blotting and real-time polymerase chain reaction, re-spectively. The intracellular distribution profile of ANXA2 expression was determined by immunofluorescence and immunohistochemistry. Short hairpin RNA target-ing ANXA2 was designed and stably transfected into MHCC97-H cells. Cells were cultured for in vitro analy-ses or subcutaneously injected as xenografts in mice for in vivo analyses. Effects of ANXA2 silencing on cell growth were assessed by cell counting kit-8 (CCK-8) as-say (in vitro ) and tumour-growth assay (in vivo ), on cell cycling was assessed by flow cytometry and propidium iodide staining (in vitro ), and on invasion and migration potential were assessed by transwell assay and wound-healing assay, respectively (both in vitro ). RESULTS: The MHCC97-H cells, which are known to have high metastasis potential, showed the highest lev-el of ANXA2 expression among the four HCC cell types examined; compared to the LO2 cells, the MHCC97-H expression level was 8-times higher. The ANXA2 expres-sion was effectively inhibited (about 80%) by ANXA2-specific small hairpin RNA (shRNA). ANXA2 expression in the MHCC97-H cells was mainly localized to the cel-lular membrane and cytoplasm, and some localization was detected in the nucleus. Moreover, the proliferation of MHCC97-H cells was obviously suppressed by shR-NA-mediated ANXA2 silencing in vitro , and the tumour growth inhibition rate was 38.24% in vivo . The per-centage of MHCC97-H cells in S phase dramatically de-creased (to 27.76%) under ANXA2-silenced conditions. Furthermore, ANXA2-silenced MHCC97-H cells showed lower invasiveness (percentage of invading cells de-creased to 52.16%) and suppressed migratory capacity (migration distance decreased to 63.49%). It is also worth noting that shRNA-mediated silencing of ANXA2 in the MHCC97-H cells led to abnormal apoptosis. CONCLUSION: shRNA-mediated silencing of ANXA2suppresses the invasion, migration, and tumorigenic potential of hepatoma cells, and may represent a useful target of future molecular therapies.

Monitoring the little fire ant,Wasmannia auropunctata(Roger 1863),in the early stage of its invasion in China:Predicting its geographical distribution pattern under climate change

Invasive alien ants(IAAs)are among the most aggressive,competitive,and widespread invasive alien species(IAS)worldwide.Wasmannia auropunctata,the greatest IAAs threat in the Pacific region and listed in“100 of the world’s worst IAS”,has established itself in many countries and on islands worldwide.Wild populations of W.auropunctata were recently reported in southeastern China,representing a tremendous potential threat to China’s agricultural,economic,environmental,public health,and social well-being.Estimating the potential geographical distribution(PGD)of W.auropunctata in China can illustrate areas that may potentially face invasion risk.Therefore,based on the global distribution records of W.auropunctata and bioclimatic variables,we predicted the geographical distribution pattern of W.auropunctata in China under the effects of climate change using an ensemble model(EM).Our findings showed that artificial neural network(ANN),flexible discriminant analysis(FDA),gradient boosting model(GBM),Random Forest(RF)were more accurate than categorical regression tree analysis(CTA),generalized linear model(GLM),maximum entropy model(MaxEnt)and surface distance envelope(SRE).The mean TSS values of ANN,FDA,GBM,and RF were 0.820,0.810,0.843,and 0.857,respectively,and the mean AUC values were 0.946,0.954,0.968,and 0.979,respectively.The mean TSS and AUC values of EM were 0.882 and 0.972,respectively,indicating that the prediction results with EM were more reliable than those with the single model.The PGD of W.auropunctata in China is mainly located in southern China under current and future climate change.Under climate change,the PGD of W.auropunctata in China will expand to higher-latitude areas.The annual temperature range(bio7)and mean temperature of the warmest quarter(bio10)were the most significant variables affecting the PGD of W.auropunctata in China.The PGD of W.auropunctata in China was mainly attributed to temperature variables,such as the annual temperature range(bio7)and the mean temperature of the warmest quarter(bio10).The populations of W.auropunctata in southern China have broad potential invasion areas.Developing strategies for the early warning,monitoring,prevention,and control of W.auropunctata in southern China requires more attention.

Bio-Morphological Characters of Alien Legume Species, Influencing Their Invasion in Natural Plant Communities

Five alien legume species, actively invading in natural plant communities in European part of Russia, were studied—Lupinus polyphyllus Lindl., Galega orientalis Lam., Robinia pseudoacacia L., Amorpha fruticosa L. and Caragana arborescens L. Distribution ranges (considering their invasive status) were mapped. Not a single bio-morphological character to forecast invasion success in natural plant communities within secondary distribution range was found. The data of key value/importance for explaining invasive success of the species studied were obtained. Two hypotheses— Propagule Pressure and Evolution of Invasiveness—were critically analyzed in view of the received data.

Nitrogen addition frequency and propagule pressure influence Solidago canadensis invasion into native plant community

Introduction:Propagule pressure(i.e.,the number of propagules)has long been recognized to play an essential role in plant invasion.But it is not clear whether propagule pressure influences the invasion of exotic plants into native plant communities when different frequencies of nitrogen are added.Method:We established an experiment with three plant communities that included native plant communities alone(four grasses,two legumes and two forbs)or native plant communities with one or five invasive plants,Solidago canadensis,under three frequencies of nitrogen addition(no addition or low or high addition with the same amount).Results:High propagule pressure significantly enhanced the biomass and relative dominance index of S.canadensis.Moreover,high propagule pressure only decreased the total and aboveground biomass of the legumes.However,the competitive effect between S.canadensis and the native community and biomass of the whole native community varied according to different frequencies.Conclusion:Overall,high propagule pressure encouraged invasion by S.canadensis,while alow nitrogen frequency was advantageous for the native community to resist invasion in this experiment.The results provide a scientific basis to manage and control the invasion of S.canadensis.

Natural Regeneration and Biological Invasion by Pinus caribaea Morelet in Two Vereda Sites: Woody Vegetation Response

Veredas are regarded as humid complexes of ecological, water and scenery significance in Cerrado biome related to water table outcrop. Direct alterations in these environments can lead to species diversity reduction and also facilitate biological invasion processes. Thus our study aims to investigate the natural regeneration, the coverage of woody vegetation and the invasive species in two sites in secondary successional stage in the central part of Cerrado biome. Our study was carried out at two sites located at Minas Gerais state, Brazil. We established plots randomly throughout transects laid perpendicularly to the vereda’s drain line. We used the line intercept method to analyze the vegetation coverage. We set five 10-m width transects in each of both areas, perpendicular to the vereda’s drain line to evaluate the distribution of invasive alien species in the two areas. The richness did not vary between communities (U = 67.50;p = 0.061);on the other hand we found significant difference in density estimates (U = 36.50;p = 0.002). We found significant difference between categories for soil coverage in both sites. Grasses showed the highest coverage when compared to the other categories (K = 45.06;p < 0.001 for site 1 and K = 67.47;p < 0.001 for site 2). We observed a zonation tendency in site 1, with a higher native grass coverage at middle and backwards zones (K = 7.734, p < 0.05). We only found Pinus caribaea Morelet individuals in the invasive species survey. We recorded 46 Pinus caribaea individuals at site 1 and 116 Pinus caribaea individuals at site 2. From the latter group we found 13 individuals within the vereda and 103 in the surrounding vegetation. Both sites have a high natural woody plant regeneration potential although the presence of alien grasses in the veredas’ edge and Pinus caribaea all over the humidity gradient highlights the vulnerability of these areas to biological invasion.

Are some communities of the coastal dune zonation more susceptible to alien plant invasion?

Aims Coastal areas,and in particular coastal dunes,are ecosystems strongly affected by the invasion of alien plants.However,few attempts have ever been made to quantify alien species incidence in different communities along the coastal zonation.This work aims to analyze the distribution of alien plants along the coastal zonation of sandy shores on the Tyrrhenian coast,addressing specifically differences among plant communities in abundance of alien plants.Methods The study was performed on recent dunes(Holocene)of the central western coast of Italy.We selected dune landscapes where invasion processes were particularly evident.Vegetation plots were randomly sampled and through cluster analysis,we identified six plant communities corresponding to the typical zonation described for the Tyrrhenian sandy coast of Central Italy.We evaluated and compared frequency and abundance of invasion in these different communities.Further,we investigated how propagule pressure(measured using as proxy human structures)contributed to the observed invasion patterns.Important Findings We found a relatively low total number of aliens but also a differential distribution pattern and strong abundance of some of the aliens in specific sectors of the vegetation zonation.The perennial community of transition dunes appears most affected by invasion processes,related almost exclusively to the frequent and widespread Carpobrotus aff.acinaciformis.This alien species reaches high cover values,apparently lowering cover of native species of transition dune plant communities.Higher levels of invasion in the transition dune can be partially explained because of greater propagule pressure in this section of the dune profile.Our findings thus have important conservation and management implications since transition dune communities with Crucianella maritima are rare and protected(sensu Habitat 92/43/EEC Directive)along the entire Italian coast.

Multiple mechanisms in woodland plant species invasion

Aims A plethora of theories explain species invasion,yet when tested in isolation,support or falsification becomes contingent on study species,system and approach.Our objective was to examine com-munity-level species invasion as a function of multiple competing hypotheses.Methods We used data from>3500 woodland plant species in 2750 plots in 49 national parks in eastern US deciduous forests to test multiple competing theories of species invasion:competition,empty niche,propagule pressure and latitude matching.We also tested interac-tions with residence time to account for non-native species naturali-zation and spread since arrival.Important Findings The non-native herbs generally thrived at latitudes similar to those from which they originated,but not necessarily where they were originally introduced to the eastern US.Overall,we found that each hypothesis explained at least some aspect of woodland plant species invasion,but examining them simultaneously allowed assessment of their relative strengths and interactions.Our results suggested that residence time is a strong predictor of non-native woodland plant success,particularly as it interacts with other mechanisms of inva-sion,such as competition(abundance of native woodland plants),climate matching(similar invaded latitude as home range),prop-agule pressure(distance to putative seed sources)and empty niche(relatedness to native plants).We found that initial barriers,such as distance from propagule source or suboptimal habitat,were over-come,as was resistance from native relatives.However,the biggest challenge for the non-native woodland plants appeared to be time,as they declined after~1 to 2 centuries.

Most patients with non-hypertensive diseases at a critical care resuscitation unit require arterial pressure monitoring: a prospective observational study

BACKGROUND:Blood pressure(BP)monitoring is essential for patient care.Invasive arterial BP(IABP)is more accurate than non-invasive BP(NIBP),although the clinical signifi cance of this diff erence is unknown.We hypothesized that IABP would result in a change of management(COM)among patients with non-hypertensive diseases in the acute phase of resuscitation.METHODS:This prospective study included adults admitted to the Critical Care Resuscitation Unit(CCRU)with non-hypertensive disease from February 1,2019,to May 31,2021.Management plans to maintain a mean arterial pressure>65 mmHg(1 mmHg=0.133 kPa)were recorded in real time for both NIBP and IABP measurements.A COM was defined as a discrepancy between IABP and NIBP that resulted in an increase/decrease or addition/discontinuation of a medication/infusion.Classification and regression tree analysis identified significant variables associated with a COM and assigned relative variable importance(RVI)values.RESULTS:Among the 206 patients analyzed,a COM occurred in 94(45.6%[94/206])patients.The most common COM was an increase in current infusion dosages(40 patients,19.4%).Patients receiving norepinephrine at arterial cannulation were more likely to have a COM compared with those without(45[47.9%]vs.32[28.6%],P=0.004).Receiving norepinephrine(relative variable importance[RVI]100%)was the most signifi cant factor associated with a COM.No complications were identifi ed with IABP use.CONCLUSION:A COM occurred in 94(45.6%)non-hypertensive patients in the CCRU.Receiving vasopressors was the greatest factor associated with COM.Clinicians should consider IABP monitoring more often in non-hypertensive patients requiring norepinephrine in the acute resuscitation phase.Further studies are necessary to confi rm the risk-to-benefi t ratios of IABP among these high-risk patients.

Correlation study between the changes of motor evoked potential and the improvement of spinal canal volume in minimally invasive transforaminal lumbar interbody fusion

Objective:To analyze the correlation between the amplitude changes of motor evoked potential(MEP),the 3D volume changes of spinal canal measuring by postoperative CT and the improvement rate of clinical symptoms after the spinal canal decompression in minimally invasive transforaminal lumbar interbody fusion(MIS-TLIF),and to explore the predictive value of the changes of both MEP amplitude and spinal canal volume in the assessment of long-term clinical prognosis in MIS-TLIF.Methods:A retrospective study of 68 patients with L4/5 spinal stenosis treated with MIS-TLIF was performed.The changes of both intraoperative MEP amplitude and 3D spinal canal volume during the spinal canal decompression,as well as the visual analogue scale(VAS)and Oswestry dysfunction index(ODI)scores in the long-term follow-up were all recorded.Results:The values of intraoperative MEP amplitude was 159.04%higher in 68 patients with MIS-TLIF after spinal canal decompression(P<0.01).The 3 postoperative 3D spinal canal volume(4.89±1.27)cm increased by 31.22%in comparison 3 with preoperative date(3.78±1.08)cm(P<0.01).The VAS and ODI scores were improved to 78.55%and 80.60%,respectively at the last follow-up(P<0.01).The improvement rate of MEP amplitude on the decompression side was positively correlated with the improvement rate of postoperative spinal canal volume(r=0.272,P=0.025).The improvement rate of postoperative spinal canal volume was positively correlated with the improvement rate of VAS and ODI at the last follow-up(r=0.656,r=0.490,P<0.01).Moreover,the improvement rate of MEP amplitude on the decompression side was also positively correlated with the improvement rate of VAS and ODI at the last follow-up(r=0.322 and 0.235,respectively,P<0.05).Conclusion:The increase of MEP amplitude after spinal canal decompression in patients with lumbar spinal stenosis treated by MIS-TLIF was closely correlated with both of the increase of spinal canal volume and the improvement of clinical symptoms.Therefore,MEP amplitude monitoring was not only the one of the important monitoring methods for predicting the prognosis of MIS-TLIF but also the reliably predictive value in the long-term clinical prognosis in MIS-TLIF.

四川盆地PT1井区海相地层工程地质特征研究

PT1井区超深海相地层钻井过程中气侵和井漏频发,严重制约该区块的安全高效开发。文章基于有效应力法,利用最优化方法开展了海相地层孔隙压力评价;结合断层和裂缝发育程度探讨了PT1井区气侵和井漏纵横向分布特征。结果表明,受异常高压和裂缝发育程度的双重影响,气侵主要发生在龙潭组,占比达40%;井漏主要发生在筇竹寺组和灯影组,占总漏失量的80%以上。气侵与异常压力、裂缝发育程度、断裂情况等因素有关,异常高压是气侵内在的原因,而断裂带和发育的构造裂缝为气侵提供了必要条件。井漏层位和漏失量主要受一、二级大型断裂带控制,不同断裂带的影响具有显著的叠加效应,断裂带附近发育的构造裂缝为漏失提供了通道,距离F I7断裂越近越易出现漏失,且漏失量越大。研究成果为PT1井区钻井过程井下复杂预防和处理提供了科学支撑。

房角切开术围手术期前房出血及术后一过性眼压升高管理方案专家推荐意见

房角切开术(GT)是一种安全、有效的微创青光眼手术(MIGS),在我国已得到广泛应用,适用于治疗原发性开角型青光眼和中晚期原发性闭角型青光眼。尽管GT手术的安全性较高,但前房出血和一过性眼压升高仍然是GT术后常见的并发症。然而,在处理这类问题时,临床医生缺乏统一的标准化规范。这种情况不仅可能影响临床医生对手术效果的判断,还可能对手术预后产生不利影响。因此,有必要制定详细、完整的围手术期前房出血及术后一过性眼压升高管理方案,指导临床医生合理、规范地处理并发症,推动MIGS的进一步发展和完善。为此国内部分青光眼治疗专家及广东省医学会眼科学分会青光眼学组相关专家共同回顾既往文献,通过反复讨论,对GT围手术期前房出血和术后一过性眼压升高的管理方案达成一致性推荐意见,包括围手术期前房出血的定义、高危因素、转归,术前预防出血的方法、术中减少出血的方法、术后前房出血的管理,及一过性眼压升高的定义、发生原因、影响因素、处理及转归等方面,以供临床医生参考使用。

各向异性地层中随钻地层测试压力响应数值模拟

在随钻地层测试器测试过程中,钻井液动态侵入和地层各向异性会导致不同的压力响应特征,并直接影响地层压力测试结果的解释精度。为此,基于各向异性多孔介质渗流理论,建立了钻井液侵入条件下横观各向同性地层随钻地层测试压力响应数学模型,采用有限元方法对模型进行求解,通过与经典解析解对比进行了模型验证,并分析了渗透率各向异性、地层产状、抽吸间歇时间、抽吸探头半径对随钻地层测试压力响应的影响规律。结果表明:在抽吸前阶段,地层各向异性和地层产状对钻井液侵入导致的井周增压影响较大;在压力恢复阶段,由于井周地层增压作用的影响,测试压力响应初始值会高于原始地层压力,且压力呈先升高后降低的演化趋势;渗透率各向异性程度越小、地层倾角和倾向越小,抽吸压降阶段的压力响应初始值越大、压降值越大、压力恢复速率越低;抽吸间隔时间和抽吸探头半径对压力响应也有一定影响,抽吸间隔时间越小,测试初始压力越大,探头半径越大,压力恢复速率越快。揭示了渗透率各向异性对随钻地层测试压力响应的影响规律,研究结果可为随钻地层测试结果解释和地层参数反演提供理论依据。

注射体积对老年患者中等量基底节区脑出血微创穿刺术后临床疗效的影响

目的探讨药物总量控制下注射体积对老年患者中等量基底节区脑出血微创穿刺术后临床疗效的影响。方法以血肿最大层面长轴中心点为穿刺点,CT引导穿刺,穿刺成功后抽吸液态血肿,控制血肿腔内单次尿激酶总量4.0×10^(4)U,注射体积分别为2 mL和4 mL,根据术后CT结果调整针体深度,并根据血肿清除情况拔针。比较两组术后血肿清除率、颅内压、血肿周围水肿体积、总死亡率、并发症发生率、神经功能缺失评分(neurological deficit score,NDS)和日常生活能力评定(activities of daily living,ADL)结果。结果两组患者术后首次血肿清除率(31.17%±1.46%vs.30.50%±1.69%)、术后颅内压、总死亡率无明显差异(P均>0.05)。大体积组术后1 d(77.30%±3.75%vs.62.77%±3.89%),2 d(91.43%±4.05%vs.80.12%±4.15%),拔针前(92.35%±4.83%vs.85.9%±3.59%)血肿清除率明显高于小体积组(P<0.05)。大体积组留针时间(1.8 d±0.2 d vs.3.1 d±0.4 d,P<0.05)、尿激酶总用量(24.3×10^(4)U±2.3×10^(4)U vs.36.5×10^(4)U±4.7×10^(4)U,P<0.05)均少于小体积组(P<0.05)。大体积组术后3 d(11.33 mL±2.32 mL vs.16.45 mL±1.97 mL)、5 d(14.59 mL±2.11 mL vs.21.37 mL±2.43 mL)、7 d(22.79 mL±3.15 mL vs.30.15 mL±4.04 mL)、14 d(12.41 mL±1.95 mL vs.19.38 mL±2.47 mL)血肿周围水肿体积少于小体积组(P<0.05)。大体积组术后28 d、3个月、6个月NDS[分别为(22.35±2.49 vs.28.14±2.95)、(16.43±2.17 vs.23.81±2.56)、(11.39±1.87 vs.17.74±2.03)]及ADL评分[分别为(44.76±4.22 vs.36.15±2.36)、(62.55±3.81 vs.51.39±2.45)、(77.53±3.76 vs.60.81±4.35)]优于小体积组(P<0.05)。大体积组术后并发症发生率低于小体积组(P<0.05)。结论大体积注射法能更有效引流血肿,缩短留针时间,减少尿激酶用量,降低术后并发症发生率,改善患者预后,是老年中等量基底节区脑出血微创穿刺术后提高疗效的有效方法。

术前夜间血压与结肠癌患者腹腔镜微创全结肠系膜切除术后发生并发症的关系

目的探讨术前夜间血压和结肠癌患者腹腔镜微创全结肠系膜切除术后发生并发症的关系。方法选择2017年3月-2023年3月于该院行腹腔镜微创全结肠系膜切除术的174例结肠癌患者。将术前夜间血压下降率≥0%的患者作为下降组,例数为92例;将术前夜间血压下降率<0%的患者作为上升组,例数为82例。分别收集患者在入室时、手术开始即刻、手术开始至30 min后、手术开始至60 min后、手术开始至120 min后和手术完成时的平均动脉压(MAP)。记录患者住院期间术后并发症发生情况;采用多因素Logistic回归模型,分析结肠癌患者行腹腔镜微创全结肠系膜切除术后发生并发症的独立危险因素。结果术前,下降组糖尿病发生率与左心室质量指数明显低于上升组(P<0.05);术前24 h动态血压参数中,两组患者术前夜间舒张压与夜间收缩压比较,差异有统计学意义(P<0.05);两组患者术中MAP各时间点比较,差异均无统计学意义(P>0.05);两组患者手术开始后各时间点MAP明显低于入室时(P<0.05);下降组术后住院时急性肾损伤的发生率为10.87%,明显低于升高组的31.71%(P<0.05);多因素Logistic回归分析结果显示,术前夜间血压上升为结肠癌患者行腹腔镜微创全结肠系膜切除术后发生急性肾损伤的独立危险因素(P<0.05)。结论术前夜间血压上升为结肠癌患者行腹腔镜微创全结肠系膜切除术后发生急性肾损伤的独立危险因素。

经额定位联合颅内压监测下微创手术治疗基底节区脑出血的疗效观察

目的探讨经额简易定位软通道联合颅内压监测下微创手术治疗椭圆形基底节区脑出血的效果。方法回顾性分析2019年1月至2021年12月右江民族医学院附属医院神经外科在简易定位后经额软通道微创手术治疗的70例椭圆形基底节区脑出血患者的临床资料,根据术前是否行颅内压(ICP)监测传感器置入术将其分为监测组26例和对照组44例,比较两组患者的一般资料、血肿清除率、术后甘露醇使用时间、术后住院时间、术后出血率、术后颅内感染率、术后1个月格拉斯哥预后分级(GOS)评分、预后良好率、术后3个月改良Rankin量表(mRS)评分等指标之间的差异。结果两组患者的年龄、性别、出血量、是否中线移位、是否破入脑室等一般资料比较差异均无统计学意义(P>0.05);监测组患者的血肿清除率为(58.72±13.61)%,明显高于对照组的(50.58±15.50)%,差异有统计学意义(P<0.05);监测组患者的术后甘露醇使用时间和住院时间分别为(3.00±2.06)d、(11.31±3.82)d,明显短于对照组的(8.23±4.29)d、(14.73±6.01)d,差异均有统计学意义(P<0.05);两组患者的术后出血率、术后颅内感染率比较差异均无统计学意义(P>0.05);监测组患者术后1个月的GOS评分为(3.96±0.96)分,明显高于对照组的(3.39±1.08)分,预后良好率为69.23%,明显高于对照组的38.64%,差异均有统计学意义(P<0.05);监测组患者术后3个月的mRS评分为(0.88±1.18)分,明显低于对照组的(2.39±1.40)分,差异有统计学意义(P<0.05)。结论在经额简易定位软通道联合颅内压监测下进行椭圆形基底节区脑出血微创手术治疗,血肿清除率较高,能有效改善患者的预后。

有创颅内压监测下控制性阶梯式减压在重型颅脑损伤中的应用

目的:探讨有创颅内压监测下控制性阶梯式减压法治疗重型颅脑损伤者的应用价值。方法:选取2022年7月—2023年7月肇庆市第一人民医院收治的82例重型颅脑外伤者作为研究对象,将其分为对照组和试验组,每组各41例。患者均未行手术治疗,且处于急性期。对照组常规监测生命体征变化情况,试验组行脑室型有创颅内压监测探头植入术。比较两组颅内压、并发症发生率(迟发血肿、术后脑梗塞)、死亡率、格拉斯哥昏迷评分法(Glasgow Coma Scale,GCS)评分及预后情况。结果:术后6 h、12 h、24 h,对照组颅内压指标高于试验组,差异有统计学意义(P<0.05)。对照组术后并发症发生率高于试验组,差异有统计学意义(P<0.05)。术后24 h、72 h、7 d,两组GCS评分比较,差异无统计学意义(P>0.05)。术后3个月,对照组恢复良好率低于试验组,差异有统计学意义(P<0.05)。结论:有创颅内压监测下控制性阶梯式减压疗法应用于重型颅脑损伤患者中效果理想,可改善患者颅内压指标,降低术后并发症发生率及死亡率,患者可更快恢复意识,改善预后。

可穿戴人体无创血压测量技术

针对传统的连续无创血压测量设备结构复杂、体积大、不宜穿戴、限制患者运动等问题,设计一种可穿戴设备对人体血压进行实时无创测量。通过硬件设备同步获取心电-脉搏波信号,设计信号处理算法,提取特征点用于后续血压估计。应用MIMIC数据中心电-脉搏-血压信号计算脉搏波传导时间及射血前期,并利用最小二乘法进行线性回归分析,构建血压测量模型,实现个性化血压检测算法。实验验证结果表明:采用该技术检测人体血压时,误差的标准差在±8 mmHg(1 mmHg≈133 Pa)范围内,平均绝对误差小于5 mmHg,满足AAMI国际血压计准确性评价标准的要求。

微创表面活性物质注射技术联合无创通气在早产儿轻中度新生儿呼吸窘迫综合征中的应用

目的探讨微创表面活性物质注射(LISA)技术联合无创通气(NIPPV)在早产儿轻中度新生儿呼吸窘迫综合征(NRDS)中的应用效果。方法选取2022年3月至2023年3月江西省妇幼保健院收治的80例NRDS患儿为研究对象,采用随机数字表法分为常规组与研究组,每组各40例。其中常规组传统技术联合NIPPV进行治疗,研究组使用LISA技术联合NIPPV进行治疗,比较两组患儿疗效、血气指标[pH值、血氧分压(PaO_(2))、二氧化碳分压(PaCO_(2))及氧合指数(OI)]、临床指标[再次肺表面活性物质(PS)治疗例数、72 h内机械通气例数、机械通气时间、住院时间]及并发症发生情况。结果研究组总有效率(97.50%)高于常规组(80.00%),差异有统计学意义(P<0.05);治疗后,研究组pH、PaO_(2)及OI值均高于常规组,PaCO_(2)低于常规组,差异有统计学意义(P<0.05)。两组患儿再次PS发生率比较,差异无统计学意义(P>0.05)。研究组72 h内机械通气发生率低于常规组,机械通气时间及住院时间短于常规组,差异有统计学意义(P<0.05)。研究组并发症总发生率(5.00%)低于常规组(22.50%),差异有统计学意义(P<0.05)。结论在治疗早产儿轻中度NRDS患儿方面,LISA技术疗效确切,相较于传统技术其改善患儿血气指标的效果更加明显,同时可显著减少患儿机械通气次数及时间,缩短住院时间,并显著减少并发症的发生。

NICU早产儿无创正压通气护理质量评价指标的构建

目的:构建新生儿重症监护室(NICU)早产儿无创正压通气护理质量评价指标,为临床评价NICU早产儿无创正压通气护理质量提供依据。方法:以“结构-过程-结果”三维质量评价模型为基础,于2023年1月—4月采用德尔菲法对25名专家进行问卷函询,并通过层次分析法确立NICU早产儿无创正压通气护理质量评价指标内容及各指标权重。结果:共开展2轮函询,问卷有效回收率分别为92.0%、100.0%,专家权威系数为0.922,0.928,肯德尔和谐系数为0.156,0.180。最终构建了3级结构模式评价指标,设有一级指标3项、二级指标14项、三级指标78项。结论:构建的无创正压通气护理质量评价指标内容科学、合理,具有较强的专科特色,可为早产儿无创正压通气护理质量评价提供参考依据。

麦默通微创旋切术与传统小切口手术对乳腺良性结节患者疼痛程度及并发症的影响比较

目的:比较麦默通微创旋切术与传统小切口手术对乳腺良性结节患者疼痛程度及并发症的影响。方法:回顾性分析2019年6月—2022年6月启东市中医院收治的108例乳腺良性结节患者的临床资料,其中54例患者行麦默通微创旋切术治疗(观察组),54例患者行传统小切口手术治疗(常规组)。两组术后均随访6个月。比较两组术中出血量、手术时间、术后6、12、24 h疼痛情况[视觉模拟量表(VAS)评分],以及血流动力学每搏输出量(SV)、平均动脉压(MAP),统计并对比两组术后并发症发生情况、满意度。结果:观察组术中出血量少于常规组(P<0.05),手术时间短于常规组(P<0.05)。两组术后不同时间点间的VAS评分对比,差异有统计学意义(F_(时间)=10.254,P=0.001);两组VAS评分比较,差异有统计学意义(F_(组间)=7.854,P=0.025),观察组术后6、12、24 h的VAS评分均低于常规组(P<0.05);组间和时间不存在交互效应(F_(交互)=2.549,P=0.089)。麻醉诱导开始时,两组SV、MAP水平比较差异均无统计学意义(P>0.05);术毕、术后12 h时,两组SV、MAP水平均高于麻醉诱导开始时,但观察组SV、MAP水平均低于常规组,差异均有统计学意义(P<0.05)。观察组术后并发症发生率低于常规组(P<0.05)。观察组总满意度高于常规组(P<0.05)。结论:与传统小切口术应用于乳腺良性结节相比,麦默通微创旋切术可促进患者康复,缓解患者疼痛,对血流动力学影响较小,降低术后并发症,提高满意度。