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Rapid source inversions of the 2023 SE Türkiye earthquakes with teleseismic and strong-motion data 被引量:4
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作者 Chenyu Xu Yong Zhang +4 位作者 Sibo Hua Xu Zhang Lisheng Xu Yuntai Chen Tuncay Taymaz 《Earthquake Science》 2023年第4期316-327,共12页
We conducted rapid inversions of rupture process for the 2023 earthquake doublet occurred in SE Türkiye,the first with a magnitude of M_(W)7.8 and the second with a magnitude of M_(W)7.6,using teleseismic and str... We conducted rapid inversions of rupture process for the 2023 earthquake doublet occurred in SE Türkiye,the first with a magnitude of M_(W)7.8 and the second with a magnitude of M_(W)7.6,using teleseismic and strong-motion data.The teleseismic rupture models of the both events were obtained approximately 88 and 55 minutes after their occurrences,respectively.The rupture models indicated that the first event was an asymmetric bilateral event with ruptures mainly propagating to the northeast,while the second one was a unilateral event with ruptures propagating to the west.This information could be useful in locating the meizoseismal areas.Compared with teleseismic models,the strong-motion models showed relatively higher resolution.A noticeable difference was found for the M_(W)7.6 earthquake,for which the strong-motion models shows a bilateral event,rather than a unilateral event,but the dominant rupture direction is still westward.Nevertheless,all strong-motion models are consistent with the teleseismic models in terms of magnitudes,durations,and dominant rupture directions.This suggests that both teleseismic and strong-motion data can be used for fast determination of major source characteristics.In contrast,the strong-motion data would be preferable in future emergency responses since they are recorded earlier and have a better resolution ability on the source ruptures. 展开更多
关键词 the 2023 earthquake doublet in SE türkiye fast source inversion teleseismic inversion strong-motion inversion
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Early Diagnosis of Recurrent Optic Neuritis Using Contrast-Enhanced T2 Fluid-attenuated Inversion Recovery Imaging:a Case Report 被引量:4
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作者 Lihui Li Houbin Huang Zhiye Chen 《Chinese Medical Sciences Journal》 CAS CSCD 2018年第2期130-134,共5页
The diagnosis of the recurrent optic neuritis is commonly established clinically,and sometimes it could be challenging because the involved optic nerve does not always show significant enhancement on conventional cont... The diagnosis of the recurrent optic neuritis is commonly established clinically,and sometimes it could be challenging because the involved optic nerve does not always show significant enhancement on conventional contrast enhanced-T1 weighted imaging(CE-T1W1).In this paper,we reported a middle-aged female with early diagnosis of recurrent optic neuritis using contrast-enhanced T2 fluid-attenuated inversion recovery imaging(CET2FLAIR).The involved optic nerve presented evident enhancement on CE-T2FLAIR and no enhancement on CE-T1W1.This case suggested that the CE-T2FLAIR may be a useful diagnostic tool specifically for the recurrent optic neuritis in clinical practice. 展开更多
关键词 t2 fluid-attenuated inversion recovery imaging magnetic resonance imaging optic neuritis
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Pre-stack seismic density inversion in marine shale reservoirs in the southern Jiaoshiba area, Sichuan Basin, China 被引量:7
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作者 Yuan-Yin Zhang Zhi-Jun Jin +3 位作者 Ye-Quan Chen Xi-Wu Liu Lei Han Wu-Jun Jin 《Petroleum Science》 SCIE CAS CSCD 2018年第3期484-497,共14页
For a typical marine shale reservoir in the Jiaoshiba area, Sichuan Basin of China, P-impedance is sensitive for identifying lithology but not suitable for indicating good shale reservoirs. In comparison, density is a... For a typical marine shale reservoir in the Jiaoshiba area, Sichuan Basin of China, P-impedance is sensitive for identifying lithology but not suitable for indicating good shale reservoirs. In comparison, density is an important quantity, which is sensitive for identifying the organic-rich mud shale from non-organic-rich mud shale. Due to the poor data quality and incidence angle range, density cannot be easily inverted by directly solving the ill-posed pre-stack seismic inversion in this area. Meanwhile, the traditional density regularizations implemented by directly using the more robust P-impedance inversion tend to be inaccurate for recovering density for this shale reservoir. In this paper, we combine the P-impedance and the minus uranium to construct the pseudo-P-impedance(PIp) at well locations. The PIp is observed to be sensitive for identifying organic-rich mud shale and has a good correlation with density in this area. We employ the PIp–density relation into the pre-stack inversion framework to estimate density. Three types of regularization are tested on both numerical and field data: These are no regularization, traditional regularization and the proposed approach. It is observed that the proposed method is better for recovering the density of organic-rich mud shale in the Jiaoshiba area. 展开更多
关键词 Density inversion pseudo-p-impedance pure p-wave data REGULARIZAtION
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Numerical estimation of choice of the regularization parameter for NMR T2 inversion 被引量:2
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作者 You-Long Zou Ran-Hong Xie Alon Arad 《Petroleum Science》 SCIE CAS CSCD 2016年第2期237-246,共10页
Nuclear Magnetic inversion is the basis of NMR Resonance (NMR) T2 logging interpretation. The regularization parameter selection of the penalty term directly influences the NMR T2 inversion result. We implemented b... Nuclear Magnetic inversion is the basis of NMR Resonance (NMR) T2 logging interpretation. The regularization parameter selection of the penalty term directly influences the NMR T2 inversion result. We implemented both norm smoothing and curvature smoothing methods for NMR T2 inversion, and compared the inversion results with respect to the optimal regular- ization parameters ((Xopt) which were selected by the dis- crepancy principle (DP), generalized cross-validation (GCV), S-curve, L-curve, and the slope of L-curve methods, respectively. The numerical results indicate that the DP method can lead to an oscillating or oversmoothed solution which is caused by an inaccurately estimated noise level. The (Xopt selected by the L-curve method is occa- sionally small or large which causes an undersmoothed or oversmoothed T2 distribution. The inversion results from GCV, S-curve and the slope of L-curve methods show satisfying inversion results. The slope of the L-curve method with less computation is more suitable for NMR T2 inversion. The inverted T2 distribution from norm smoothing is better than that from curvature smoothing when the noise level is high. 展开更多
关键词 NMR t2 inversion tikhonov regularizationVariable substitution Levenberg-Marquardt method Regularization parameter selection
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Inversion of two-dimensional tidal open boundary conditions of M_2 constituent in the Bohai and Yellow Seas 被引量:2
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作者 曹安州 郭筝 吕咸青 《Chinese Journal of Oceanology and Limnology》 SCIE CAS CSCD 2012年第5期868-875,共8页
Two-dimensional tidal open boundary conditions of the M2 constituent in the Bohai and Yellow Seas(BYS) have been estimated by assimilating T/P altimeter data.During inversion,independent point(IP) strategy was used,in... Two-dimensional tidal open boundary conditions of the M2 constituent in the Bohai and Yellow Seas(BYS) have been estimated by assimilating T/P altimeter data.During inversion,independent point(IP) strategy was used,in which several IPs on the open boundary is assumed,values at these IPs can be optimized with an adjoint method,and those at other grid points are determined by linearly interpolating the values at IPs.The reasonability and feasibility of the model are tested by ideal twin experiments.In the practical experiment(PE) after assimilation,the cost function may reach 1% or less of its initial value.Mean absolute errors in amplitude and phase can be less than 5 cm and 5°,respectively,and the obtained co-chart can show the character of the M2 constituent in the BYS.The results of the PE indicate that using only two IPs on the open boundary can yield better simulated results. 展开更多
关键词 tWO-DIMENSIONAL adjoint method t/p altimeter open boundary conditions independent points
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A high resolution inversion method for fluid factor with dynamic dryrock V_(P)/V_(S) ratio squared 被引量:1
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作者 Lin Zhou Jian-Ping Liao +3 位作者 Xing-Ye Liu Pu Wang Ya-Nan Guo Jing-Ye Li 《Petroleum Science》 SCIE EI CSCD 2023年第5期2822-2834,共13页
As an important indicator parameter of fluid identification,fluid factor has always been a concern for scholars.However,when predicting Russell fluid factor or effective pore-fluid bulk modulus,it is necessary to intr... As an important indicator parameter of fluid identification,fluid factor has always been a concern for scholars.However,when predicting Russell fluid factor or effective pore-fluid bulk modulus,it is necessary to introduce a new rock skeleton parameter which is the dry-rock VP/VS ratio squared(DVRS).In the process of fluid factor calculation or inversion,the existing methods take this parameter as a static constant,which has been estimated in advance,and then apply it to the fluid factor calculation and inversion.The fluid identification analysis based on a portion of the Marmousi 2 model and numerical forward modeling test show that,taking the DVRS as a static constant will limit the identification ability of fluid factor and reduce the inversion accuracy.To solve the above problems,we proposed a new method to regard the DVRS as a dynamic variable varying with depth and lithology for the first time,then apply it to fluid factor calculation and inversion.Firstly,the exact Zoeppritz equations are rewritten into a new form containing the fluid factor and DVRS of upper and lower layers.Next,the new equations are applied to the four parameters simultaneous inversion based on the generalized nonlinear inversion(GNI)method.The testing results on a portion of the Marmousi 2 model and field data show that dynamic DVRS can significantly improve the fluid factor identification ability,effectively suppress illusion.Both synthetic and filed data tests also demonstrate that the GNI method based on Bayesian deterministic inversion(BDI)theory can successfully solve the above four parameter simultaneous inversion problem,and taking the dynamic DVRS as a target inversion parameter can effectively improve the inversion accuracy of fluid factor.All these results completely verified the feasibility and effectiveness of the proposed method. 展开更多
关键词 Fluid factor Dry-rock V_(p)/V_(S)ratio squared(DVRS) Dynamic variable Multiple parameters simultaneous inversion Generalized nonlinear inversion(GNI)
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Cardiac and non-cardiac causes of T-wave inversion in the precordial leads in adult subjects: A Dutch case series and review of the literature 被引量:7
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作者 Salah AM Said Rene Bloo +1 位作者 Ramon de Nooijer Andries Slootweg 《World Journal of Cardiology》 CAS 2015年第2期86-100,共15页
AIM: To describe the electrocardiographic(ECG) phenomena characterized by T-wave inversion in the precordial leads in adults and to highlight its differential diagnosis. METHODS: A retrospective chart review of 8 adul... AIM: To describe the electrocardiographic(ECG) phenomena characterized by T-wave inversion in the precordial leads in adults and to highlight its differential diagnosis. METHODS: A retrospective chart review of 8 adult patients who were admitted with ECG T-wave inversion in the anterior chest leads with or without prolongation of corrected QT(QTc) interval. They had different clinical conditions. Each patient underwent appropriate clinical assessment including investigation for myocardial involvement. Single and multimodality noninvasive, semi-invasive and invasive diagnostic approach were used to ascertain the diagnosis. The diagnostic assessment included biochemical investigation, cardiac and abdominal ultrasound, cerebral and chest computed tomography, nuclear medicine and coronary angiography.RESULTS: Eight adult subjects(5 females) with a mean age of 66 years(range 51 to 82) are analyzed. The etiology of T-wave inversion in the precordial leads were diverse. On admission, all patients had normal blood pressure and the ECG showed sinus rhythm. Five patients showed marked prolongation of the QTc interval. The longest QTc interval(639 ms) was found in the patient with pheochromocytoma. Giant T-wave inversion(≥ 10 mm) was found in pheochromocytoma followed by electroconvulsive therapy and finally ischemic heart disease. The deepest T-wave was measured in lead V3(5 ×). In 3 patients presented with mild T-wave inversion(patients 1, 5 and 4 mm), the QTc interval was not prolonged(432, 409 and 424 msec), respectively.CONCLUSION: T-wave inversion associated with or without QTc prolongation requires meticulous history taking, physical examination and tailored diagnostic modalities to reach rapid and correct diagnosis to establish appropriate therapeutic intervention. 展开更多
关键词 t-WAVE inversion Coronary ANGIOGRApHY pulmonary computed tomography ANGIOGRApHY Mag-netic resonance imaging Differential diagnosis
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Elastic modulus extraction based on generalized pre-stack PP–PS wave joint linear inversion 被引量:2
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作者 Ma Qi-Qi Sun Zan-Dong 《Applied Geophysics》 SCIE CSCD 2018年第3期466-480,共15页
Joint PP–PS inversion offers better accuracy and resolution than conventional P-wave inversion. P-and S-wave elastic moduli determined through data inversions are key parameters for reservoir evaluation and fluid cha... Joint PP–PS inversion offers better accuracy and resolution than conventional P-wave inversion. P-and S-wave elastic moduli determined through data inversions are key parameters for reservoir evaluation and fluid characterization. In this paper, starting with the exact Zoeppritz equation that relates P-and S-wave moduli, a coefficient that describes the reflections of P-and converted waves is established. This method effectively avoids error introduced by approximations or indirect calculations, thus improving the accuracy of the inversion results. Considering that the inversion problem is ill-posed and that the forward operator is nonlinear, prior constraints on the model parameters and modified low-frequency constraints are also introduced to the objective function to make the problem more tractable. This modified objective function is solved over many iterations to continuously optimize the background values of the velocity ratio, which increases the stability of the inversion process. Tests of various models show that the method effectively improves the accuracy and stability of extracting P and S-wave moduli from underdetermined data. This method can be applied to provide inferences for reservoir exploration and fluid extraction. 展开更多
关键词 pre-stack JOINt pppS inversion p-and S-wave moduli exact Zoeppritz equation GENERALIZED linear inversion reservoir and fl uid prediction
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v_p/v_s ESTIMATION IN TIBETAN CRUST FROM INVERSION OF SURFACE WAVE DISPERSIONS
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作者 Wang Yang 《地学前缘》 EI CAS CSCD 2000年第S1期373-373,共1页
We estimated crustal v p/ v s ratio of Tibetan Plateau by combined inversion of Love and Rayleigh wave dispersion data. It is developed by us that the joint inversion methods using both Love and Rayleigh wave dispersi... We estimated crustal v p/ v s ratio of Tibetan Plateau by combined inversion of Love and Rayleigh wave dispersion data. It is developed by us that the joint inversion methods using both Love and Rayleigh wave dispersion data. Thickness and S\|wave velocity of each sub layer are taken from Love wave dispersion data, then P\|wave velocity structure was deduced using Rayleigh wave dispersion data. Densities of sub layers were estimated by the empirical relationships between seismic velocity and rock density. Having S\| and P\|wave velocities, v p/ v s ratio is calculated for each sub layer. Six sub layers in crust of Tibetan Plateau has been identified, which are 0~8km, 8~30km, 30~40km, 40~62km, 62~68km and 68~75km respectively. The S\|wave velocity structure of the Plateau is 3 13, 3 32, 3 15, 3 92, 3 45 and 3 87 km/s for each sub layer; and P\|wave velocities are 6 00, 6 10, 5 72, 6 35, 6 78 and 6 64km/s respectively v p/ v s ratios in sub layers are 1 92, 1 84, 1 82, 1 62, 1 96 and 1 72; and corresponding Poisson ratios are 0 31, 0 29, 0 28, 0 19, 0 32 and 0 24. Our result on Poisson ratios of Tibetan crust was supported by seismic waveform modelling by Rodgers and Schwartz (1998). 展开更多
关键词 tIBEtAN plateau V p/ V s RAtIO poisson RAtIO of CRUSt surface wave inversion
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Inversion of waveforms from Xiangtang borehole seismic array for soil dynamic property
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作者 陈学良 金星 +1 位作者 陶夏新 韦永祥 《Acta Seismologica Sinica(English Edition)》 CSCD 2007年第4期425-434,共10页
In order to understand the site soil response of the Xiangtang borehole seismic array under real strong ground motion, reveal the site response, verify the technique of borehole exploration, and improve the precision ... In order to understand the site soil response of the Xiangtang borehole seismic array under real strong ground motion, reveal the site response, verify the technique of borehole exploration, and improve the precision of in-situ test and laboratory test, this paper presents a new approach, which is composed of two methods. One is the layered site seismic response method, whose layer transform matrix is always real. The other is a global-local optimization technique, which uses genetic algorithm (GA)-simplex method. An inversion of multi-component waveforms of P, SV and SH wave is carried out simultaneously. By inverting the records of three moderate and small earthquakes obtained from the Xiangtang borehole array (2^#) site, the soil dynamic characteristic parameters, including P velocity, damping ratio and frequency-dependent coefficient b, which has not been given in previous literatures, are calculated. The results show that the soil S wave velocity of the Xiangtang 2^# borehole is generally greater than that obtained from the 1994 in-situ test, and is close to the velocity of the 3^# borehole, which is more than 200 m away from the 2^# borehole. Meanwhile, perceptible soil nonlinear behavior under peak ground motion of about 60×10^-2 m/s^2 is detected by the inversion analysis. The presented method can be used for studying the soil response of other borehole array sites. 展开更多
关键词 Xiangtang borehole array inversion analysis soil response p and S wave velocity damping ratio
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Preoperative Atrial Fibrillation and T-Wave Inversion Associated with Postoperative Pulmonary Edema
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作者 Thomas Hong Benson George +1 位作者 Mohamad Hashim Dennis E. Feierman 《Open Journal of Anesthesiology》 2016年第10期181-185,共5页
Background: Atrial fibrillation (AF) and T wave inversion (TWI) are occasionally found on pre-operative electrocardiograms (EKG) of patients with long standing hy-pertension (HTN) associated with left ventricular hype... Background: Atrial fibrillation (AF) and T wave inversion (TWI) are occasionally found on pre-operative electrocardiograms (EKG) of patients with long standing hy-pertension (HTN) associated with left ventricular hypertrophy (LVH). Each of these two pathologies, AF and LVH, can impose negative hemodynamic effects on the car-diac function. Case: We present a case of a patient with AF, TWI and long standing HTN that went into post-operative pulmonary edema. Conclusion: Even returning to base line poorly controlled hypertension, and even more so during a hypertensive cri-sis, their concurrent presence may inflict even more detrimental effect manifested by elevation of pulmonary venous pressure and pulmonary edema. 展开更多
关键词 pulmonary Edema Atrial Fibrillation t Wave inversion
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An algorithm for multi-exponential inversion of T_2 spectrum in nuclear magnetic resonance
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作者 HAN Chunjiang REN Li WANG Zhuwen 《Global Geology》 2014年第2期105-109,共5页
NMR logging can provide the permeability parameter and abundant stratigraphical information such as total porosity,oil,gas and water saturation,oil viscosity,etc. And these physical parameters can be obtained by T2 sp... NMR logging can provide the permeability parameter and abundant stratigraphical information such as total porosity,oil,gas and water saturation,oil viscosity,etc. And these physical parameters can be obtained by T2 spectrum inversion. NMR inversion is an important part in logging interpretation. The authors describe a multi-exponential inversion algorithm,solid iteration redress technique( SIRT),and apply the algorithm in real data and compare the results with those based on singular value decomposition( SVD). It shows that SIRT algorithm is easier to be understood and implemented,and the time spent in SIRT is much shorter than that of SVD algorithm. And the non-negative property of T2 spectrum is much easier to be implemented. It can match the results based on SVD very well. SIRT algorithm can be used in T2 spectrum inversion for NMR analysis. 展开更多
关键词 NMR logging t2spectrum SIRt algorithm inversion
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A Time-space Domain Backprojection Method for Ray Inversion in Layered Medium
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作者 马兴瑞 陶良 黄文虎 《Journal of Harbin Institute of Technology(New Series)》 EI CAS 1994年第1期85-87,共3页
The distribution of incident angles of the discrete projection data may be uneven when the wave rays in random medium are nonlinear, moreover, the far field condition will be broken down when the inversion region is l... The distribution of incident angles of the discrete projection data may be uneven when the wave rays in random medium are nonlinear, moreover, the far field condition will be broken down when the inversion region is large. Thus the inversion result will be distorted unavoidably in many ray inversion methods. In order to eliminate these influences, a new t-x(time-spac) domain backprojection method compensating the density of the distribution of incident angles is developed. The paper provides the principle of the incident angle compensation and simulation result of a point defect in layered medium. 展开更多
关键词 ss: INCIDENt angle compensation t-x DOMAIN backprojection RAY inversion in HOMOGENEOUS MEDIUM
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Samarium(II) Diiodide Induced Polarity Inversion of π-Allyl Palladium Complexes: The Formation of Allylic Selenides
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作者 XinJianZHAO HuaRongZHAO 《Chinese Chemical Letters》 SCIE CAS CSCD 2002年第5期396-397,共2页
Allylic acetates were reduced with Pd(0)-SmI2 in the presence of ArSeBr to form corresponding allylic selenides in good yields.
关键词 Allyl acetates allylic selenides arylselenenyl bromides palladium complexes polarity inversion. Samarium(II) Diiodide Induced polarity inversion of p-Allyl palladium Complexes: the Formation of Allylic Selenides Xin Jian ZHAO1 Hua Rong ZH
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利用Inverse PCR快速筛选单个T-DNA拷贝转基因水稻植株的方法 被引量:7
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作者 章冰 黄健秋 卫志明 《实验生物学报》 CSCD 1999年第2期207-211,共5页
为了获得单个T-DNA插入拷贝的植株, 我们建立了一套利用Inverse PCR(IPCR)快速检测转基因水稻中T-DNA拷贝数的方法。用IPCR的方法可以扩增出与已知T-DNA序列相邻的水稻基因组DNA未知序列,由此推测转基因水稻植株中T-DNA的拷贝数。我们共... 为了获得单个T-DNA插入拷贝的植株, 我们建立了一套利用Inverse PCR(IPCR)快速检测转基因水稻中T-DNA拷贝数的方法。用IPCR的方法可以扩增出与已知T-DNA序列相邻的水稻基因组DNA未知序列,由此推测转基因水稻植株中T-DNA的拷贝数。我们共对15个转化株系20棵不同植株的DNA进行了IPCR检测。其中12株表现为T-DNA单拷贝插入,3株为双拷贝插入,1株为三拷贝插入。另外4株未检测到T-DNA插入拷贝。IPCR分析结果经过Southern杂交和测序的验证。 展开更多
关键词 水稻 转基因 t-DNA拷贝 inversepCR
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口腔扁平苔藓患者血清miR-155-5p、SOCS1 mRNA水平与病损程度及Th17/Treg失衡的关系
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作者 李玲玲 梁伟腾 后珉红 《山东医药》 CAS 2024年第13期26-29,35,共5页
目的探讨口腔扁平苔藓(OLP)患者血清微小核糖核酸-155-5p(miR-155-5p)、细胞因子信号传导抑制蛋白1(SOCS1)mRNA水平与病损程度及辅助性T细胞17(Th17)/调节性T细胞(Treg)失衡的关系。方法选择OLP患者76例(观察组),其中非糜烂型28例、糜烂... 目的探讨口腔扁平苔藓(OLP)患者血清微小核糖核酸-155-5p(miR-155-5p)、细胞因子信号传导抑制蛋白1(SOCS1)mRNA水平与病损程度及辅助性T细胞17(Th17)/调节性T细胞(Treg)失衡的关系。方法选择OLP患者76例(观察组),其中非糜烂型28例、糜烂型48例(轻中度糜烂29例、重度糜烂19例),同期随机另选体检健康的志愿者50例(对照组),采集所有研究对象外周静脉血,采用实时荧光定量聚合酶链式反应检测血清miR-155-5p、SOCS1 mRNA,采用流式细胞术检测Th17、Treg比例并计算Th17/Treg。采用Pearson/Spearman相关分析法分析OLP患者血清miR-155-5p水平与SOCS1 mRNA水平的关系以及二者与外周血Th17、Treg比例和Th17/Treg的关系。结果与对照组比较,观察组血清miR-155-5p水平以及外周血Th17比例和Th17/Treg升高,血清SOCS1 mRNA水平和外周血Treg比例降低(P均<0.05)。非糜烂型OLP及糜烂型OLP轻中度糜烂、重度糜烂患者血清miR-155-5p水平以及外周血Th17比例和Th17/Treg逐渐升高,血清SOCS1 mRNA水平和外周血Treg比例逐渐降低(P均<0.05)。相关性分析显示,OLP患者血清miR-155-5p水平与血清SOCS1 mRNA水平呈负相关关系(r=-0.809,P<0.01);血清miR-155-5p水平与外周血Th17比例、Th17/Treg均呈正相关关系(r/rs分别为0.819、0.820,P均<0.05),与Treg比例呈负相关关系(r=-0.735,P<0.05);血清SOCS1 mRNA水平与外周血Th17比例、Th17/Treg均呈负相关关系(r/rs分别为-0.770、-0.790,P均<0.05),与Treg比例呈正相关关系(r=0.733,P<0.05)。结论OLP患者血清miR-155-5p水平升高、血清SOCS1 mRNA水平降低,二者水平变化与病损程度加重和Th17/Treg失衡密切相关。 展开更多
关键词 口腔扁平苔藓 微小核糖核酸-155-5p 细胞因子信号传导抑制蛋白1 病损程度 辅助性t细胞17/调节性t细胞失衡
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bta-miR-27a-5p的生物信息学分析及其对3T3-L1前脂肪细胞分化的影响
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作者 李鑫 乔俊杰 +6 位作者 吴金瑞 侯中一 孟聖博 杜志龙 许会芬 李明 蔡含芳 《黑龙江畜牧兽医》 CAS 北大核心 2024年第13期1-9,111,共10页
为了探究bta-miR-27a-5p的生物学特性及其对3T3-L1前脂肪细胞分化的影响,试验利用miRBase数据库对不同物种间miR-27a-5p的保守性进行分析,并构建了miR-27a-5p的系统进化树;利用TargetScan和miRWalk在线网站预测bta-miR-27a-5p的靶基因,... 为了探究bta-miR-27a-5p的生物学特性及其对3T3-L1前脂肪细胞分化的影响,试验利用miRBase数据库对不同物种间miR-27a-5p的保守性进行分析,并构建了miR-27a-5p的系统进化树;利用TargetScan和miRWalk在线网站预测bta-miR-27a-5p的靶基因,并对靶基因进行GO功能注释和KEGG信号通路分析;利用实时荧光定量PCR方法检测在不同月龄夏南牛不同器官中bta-miR-27a-5p基因的相对表达量;最后通过实时荧光定量PCR方法、Western-blot检测、三酰甘油浓度测定和油红O染色探究过表达bta-miR-27a-5p后对3T3-L1前脂肪细胞分化的影响。结果表明:不同物种miR-27a-5p的成熟序列保守性较高;牛miR-27a-5p基因与山羊的亲缘关系最近,与非洲爪蟾的亲缘关系最远;bta-miR-27a-5p靶基因主要在细胞过程、生物过程、细胞、细胞部分、结合等GO条目上富集,并富集在胰岛素抵抗、细胞衰老、脂肪细胞因子等信号通路中;bta-miR-27a-5p基因可在夏南牛的多个器官中表达,且12月龄夏南牛脂肪组织中的相对表达量均显著低于0月龄和24月龄(P<0.05);过表达bta-miR-27a-5p基因可显著或极显著抑制C/EBPα、PPARγ和FABP4基因和蛋白质表达(P<0.05或P<0.01),并显著或极显著降低3T3-L1前脂肪细胞内脂滴和三酰甘油浓度(P<0.05或P<0.01)。说明bta-miR-27a-5p在不同物种间高度保守,在不同月龄夏南牛脂肪组织中显著差异表达,并且能抑制3T3-L1前脂肪细胞分化,bta-miR-27a-5p基因可能是调控牛脂肪生成的候选miRNA。 展开更多
关键词 bta-miR-27a-5p 靶基因 生物信息学分析 夏南牛 表达分析 3t3-L1前脂肪细胞
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T23/P23钢高强匹配焊缝金属接头再热裂纹表征
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作者 孙咸 《钢管》 CAS 2024年第2期17-27,共11页
综述了T23/P23钢高强匹配焊缝金属接头再热裂纹的特征。结果表明,T23/P23耐热钢高强匹配接头再热裂纹倾向明显,焊后状态高强匹配接头具有较高的强度和可以接受的塑性和韧性。接头显微组织中的HAZ粗晶区碳化物析出、晶界的滑移及晶界的... 综述了T23/P23钢高强匹配焊缝金属接头再热裂纹的特征。结果表明,T23/P23耐热钢高强匹配接头再热裂纹倾向明显,焊后状态高强匹配接头具有较高的强度和可以接受的塑性和韧性。接头显微组织中的HAZ粗晶区碳化物析出、晶界的滑移及晶界的脆化是再热裂纹产生的必要条件;影响HAZ组织的主要因素是母材中碳化物形成元素和残留元素的种类及含量。随接头拘束应力增大,再热温度升高时再热裂纹倾向增大。T23/P23钢接头中氢的行为是产生再热裂纹不可忽视的影响因素。生产中常用低应力化和低氢化综合工艺及参数,以及新型专用焊接材料等措施,使接头中的应力水平降低,氢含量最小化,控制再热裂纹形成条件,并获得了较满意的效果。 展开更多
关键词 t23/p23钢 高强匹配接头 再热裂纹 铁研试验 扩散氢
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龙血素B通过P38MAPK信号通路调控MC3T3-E1成骨分化和骨形成的机制
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作者 李经堂 涂乐佳 +2 位作者 陈淼 魏鹏 周璟瑜 《医学理论与实践》 2024年第7期1081-1084,共4页
目的:探究龙血素B对小鼠胚胎成骨细胞前体细胞(MC3T3-E1细胞)成骨分化和骨形成的影响以及其通过P38MAPK信号通路调控成骨分化和骨形成的机制。方法:培养MC3T3-E1细胞,加入不同浓度(0、15、30、60、90、120μmol/L)龙血素B,采用CCK8法和... 目的:探究龙血素B对小鼠胚胎成骨细胞前体细胞(MC3T3-E1细胞)成骨分化和骨形成的影响以及其通过P38MAPK信号通路调控成骨分化和骨形成的机制。方法:培养MC3T3-E1细胞,加入不同浓度(0、15、30、60、90、120μmol/L)龙血素B,采用CCK8法和流式细胞术(FCM)检测不同时间(24h、48h、72h)MC3T3-E1细胞增殖能力和凋亡情况,确定龙血素B促MC3T3-E1细胞成骨最佳的药物浓度及作用时间。培养MC3T3-E1细胞并分为空白组(不作干预),龙血素B组(加入龙血素B共培养),龙血素B+阻断剂组(加入龙血素B和P38抑制剂SB203580共培养),进行干预实验;采用碱性磷酸酶(ALP)活性检测试剂盒测定各组细胞ALP活性,qRT-PCR法检测各组细胞骨桥蛋白(OPN)基因、骨钙素(OCN)基因、骨唾液蛋白(BSP)基因表达水平,茜素红染色法观察各组细胞骨形成能力。结果:龙血素B可促进MC3T3-E1细胞增殖并抑制其凋亡,效果与浓度和干预时间相关,在90μmol/L浓度下干预48h促MC3T3-E1细胞生长作用最强;干预结束后第1、3、5天,龙血素B组细胞ALP活性较空白组升高,龙血素B+阻断剂组细胞ALP活性较龙血素B组降低(P<0.05);龙血素B组细胞OPN、OCN、BSP基因表达水平较空白组升高,龙血素B+阻断剂组细胞OPN、OCN、BSP基因表达水平较龙血素B组降低(P<0.05);干预结束后第21天,龙血素B组细胞钙化结节区域面积较空白组增大,龙血素B+阻断剂组细胞钙化结节区域面积较龙血素B组减小(P<0.05)。结论:龙血素B可提高MC3T3-E1细胞增殖活性,并通过激活P38MAPK信号通路促进MC3T3-E1细胞成骨分化和骨形成。 展开更多
关键词 龙血素B p38MApK信号通路 MC3t3-E1细胞 成骨分化 骨形成
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华北克拉通吉南夹皮沟新太古代石榴黑云斜长片麻岩的变质P-T-t演化及构造意义 被引量:1
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作者 韩香巧 钱加慧 +3 位作者 卢成森 尹常青 张健 高彭 《岩石学报》 SCIE EI CAS CSCD 北大核心 2024年第11期3379-3397,共19页
华北克拉通东部陆块新太古代末期的构造体制是近年来备受学界关注的热点议题。本文对地处华北克拉通东部陆块东北部的吉南夹皮沟石榴黑云斜长片麻岩进行了详细的岩相学、矿物化学、变质相平衡模拟、传统地质温压计和锆石U-Pb年代学研究... 华北克拉通东部陆块新太古代末期的构造体制是近年来备受学界关注的热点议题。本文对地处华北克拉通东部陆块东北部的吉南夹皮沟石榴黑云斜长片麻岩进行了详细的岩相学、矿物化学、变质相平衡模拟、传统地质温压计和锆石U-Pb年代学研究,以明确其变质P-T-t演化,探讨构造过程和动力学机制。石榴黑云斜长片麻岩主要由石榴石、黑云母、斜长石、角闪石、石英和少量钛铁矿组成,记录了逆时针P-T轨迹,可以划分为三个变质阶段。岩石第一阶段的矿物组合为黑云母+斜长石+角闪石+石英+钛铁矿(+熔体)(Bt 1+Pl 1+Amp 1+Qz+Ilm(+L)),记录的温压条件为790~854℃和0.55~0.88GPa,之后通过升压略降温到达压力峰期阶段,在这个过程中角闪石分解、石榴石生长、基质型斜长石的X_(An)值从核到边降低。第二阶段的矿物组合为石榴石+黑云母+斜长石+角闪石+石英+钛铁矿(+熔体)(Grt+Bt_(2)+Pl_(2)(核部)+Amp_(2)+Qz+Ilm(+L)),相平衡模拟获得该阶段的温压条件为670~745℃和1.10~1.24GPa,传统地质温压计的结果为707~771℃和0.99~1.18GPa,两者可相互佐证,可能代表了岩石的压力峰期阶段。第三阶段的矿物组合为黑云母+斜长石+石英+钛铁矿(Bt_(3)+Pl_(2)(边部)+Qz+Ilm),形成于压力峰期后的降温降压过程,石榴石边部分解形成斜长石,该类斜长石的X_(An)值从核到边逐渐升高。变质锆石U-Pb定年获得2483±8Ma(MSWD=3.2)的年龄,结合稀土配分模式和锆石Ti温度计计算结果,将该年龄解释为岩石压力峰期阶段前的变质时代。考虑到吉南变质地体新太古代末期岩浆活动与变质作用的时间重叠性、区域上的“穹窿-龙骨”构造样式、前人的变质作用资料以及石榴黑云斜长片麻岩记录的升压轨迹,本文认为吉南新太古代末期变质作用与重力凹沉构造有关。 展开更多
关键词 石榴黑云斜长片麻岩 变质p-t-t演化 新太古代 重力凹沉构造 华北克拉通
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