The vitamin C(VC) in crops was successfully determined using ascorbate oxidase(AO) electrochemical biosensor based on the biocompatible poly(3,4-ethylenedioxythiophene)(PEDOT) matrices,which was easily prepare...The vitamin C(VC) in crops was successfully determined using ascorbate oxidase(AO) electrochemical biosensor based on the biocompatible poly(3,4-ethylenedioxythiophene)(PEDOT) matrices,which was easily prepared by one-step electrodeposition technique in ionic liquid microemulsions.The fabricated biosensor displayed excellent bioelectrocatalytic performance to the oxidation of VC,wide linear range,low detection limit,fast response time,good operational and storage stability,the good results of the determination of VC in vegetable crops indicated that the fabricated biosensor will be a good candidate for the physiological and biochemical studies of crops in near future.展开更多
基金supported by NSFC(Nos50963002 and 51073074)Key Projects in the National Science& Technology Pillar Program in the Eleventh Five-year Plan Period of China(Nos2006BAD02A04 and 2006BAD01A01)Jiangxi Provincial Department of Education(NosGJJ11590 and GJJ10678)
文摘The vitamin C(VC) in crops was successfully determined using ascorbate oxidase(AO) electrochemical biosensor based on the biocompatible poly(3,4-ethylenedioxythiophene)(PEDOT) matrices,which was easily prepared by one-step electrodeposition technique in ionic liquid microemulsions.The fabricated biosensor displayed excellent bioelectrocatalytic performance to the oxidation of VC,wide linear range,low detection limit,fast response time,good operational and storage stability,the good results of the determination of VC in vegetable crops indicated that the fabricated biosensor will be a good candidate for the physiological and biochemical studies of crops in near future.