黄芪甲苷调控IRE-1α信号通路改善糖尿病肾病足细胞内质网应激

目的:探讨黄芪甲苷(astragalosideⅣ,AS-Ⅳ)调控肌醇依赖性激酶1α(inositol requiring enzyme 1α,IRE-1α)信号通路,减轻足细胞内质网应激,改善糖尿病肾病(diabetic nephropathy,DN)足细胞损伤。方法:(1)培养大鼠足细胞,将其分为正常组(5.5 mmol/L葡萄糖)、高糖组(30 mmol/L葡萄糖)、AS-Ⅳ组(20μmmol/L黄芪甲苷)、IRE-1α抑制剂组(20μmol/L STF-083010),干预24 h后收集细胞,采用RT-PCR检测内质网应激指标葡萄糖调节蛋白78(glucose-regulated protein 78,GRP78)、IRE-1α、肿瘤坏死因子相关受体2(tumor necrosis factor receptor-associated factor 2,TRAF-2)、IKK-α基因表达。(2)40只SPF级雄性SD大鼠,随机分为正常组10只和造模组30只。喂养1周后,采用链脲佐菌素(STZ)55 mg/kg腹腔注射,连续3 d,造模成功后随机分为DN组、AS-Ⅳ组(AS-Ⅳ40 mg·kg^(-1)·d^(-1)灌胃)、IRE-1α抑制剂组(STF-083010 10 mg·kg^(-1)·d^(-1)灌胃),干预8周后收集各组大鼠24 h尿液,ELISA测定尿蛋白定量,收集各组大鼠肾组织,行HE染色,免疫组化及RT-PCR检测GRP78、IRE-1α、TRAF-2、IKK-α表达。结果:(1)与正常组比较,高糖组足细胞GRP78、IRE-1α、TRAF-2、IKK-α mRNA表达升高(P<0.05);与高糖组相比,AS-Ⅳ组和IRE-1α抑制剂组表达下降(P<0.05);(2)与正常组比较,DN组大鼠24 h尿蛋白定量升高(P<0.05),病理HE染色可见肾小球肥大、系膜增生,基底膜增厚;与DN组比较,AS-Ⅳ组、IRE-1α抑制剂组大鼠24 h尿蛋白定量及病理改变减轻(P<0.05);(3)免疫组化结果显示,与正常组比较,DN组大鼠肾组织GRP78、IRE-1α表达增高(P<0.05);RT-PCR结果显示,与正常组比较,DN组GRP78、IRE-1α、TRAF-2、IKK-α mRNA表达增高(P<0.05);与DN组比较,AS-Ⅳ组、IRE-1α抑制剂组上述表达下降(P<0.05)。结论:AS-Ⅳ通过调控IRE-1α信号通路,减轻内质网应激,改善糖尿病肾病足细胞损伤。

Submergence Analysis Using Geo-Informatics Technology for Proposed Dam Reservoirs of Par-Tapi-Narmada River Link Project, Gujarat State, India

The Par-Tapi-Narmada river link envisages transfer of surplus water from west flowing rivers between Par and Tapi in Gujarat State, India to water deficit areas in North Gujarat. The scheme is located mainly in southern Gujarat but it also covers part of the areas of Maharashtra, North of Mumbai on the Western Ghats of India. The main aim of Par-Tapi-Narmada link is to transfer the surplus waters of Par, Auranga, Ambica and Purna River basins to take over part of Narmada Canal command (Miyagam branch) after providing enroute irrigation. It is proposed that water saved in Sardar Sarovar Project, as a result of this transfer, would be taken further northwards to benefit water scarce areas of north Gujarat and also westwards in Saurashtra and Kutch regions. The construction of seven reservoirs on Par-Tapi-Narmada River Link Project would affect land use/land cover, settlements and infrastructure facilities within and around reservoir area. Thus, the submergence impact analysis of all the seven reservoirs of this project have been carried out by using remote sensing and GIS techniques for planning and designing of the structures. Out of the seven reservoirs, the paper discusses submergence analysis of Kelwan Dam reservoir which is located in geologically complex region of the Dangs district. The study attempts to assess the present problems of submergence of land, forest, agriculture, settlements and infrastructure facilities by using GIS techniques for taking alternative remedial measures prior and during construction of the dams.

铱(IV)离子与人血丙种球蛋白的作用研究

在 0.1 mol?L-1醋酸-醋酸钠(pH 5.0)体系中, 采用紫外吸收光谱、荧光光谱及同步荧光光谱法研究了人血丙种球蛋白(gamma seroglobulinum humanum, 简称 GSH)与铱(IV)离子的相互作用. 结果表明, Ir(IV)离子使人血丙种球蛋白的构象发生了改变, α-螺旋含量减少, 并且用同步荧光光谱发现 Ir(IV)离子与人血丙种球蛋白的作用位点更接近于色氨酸,从而使色氨酸残基的疏水性略有减小. 荧光光谱结果表明Ir(IV)对人血丙种球蛋白内源荧光(342 nm)产生了较强的荧光猝灭作用, 根据不同温度下 Ir(IV)对人血丙种球蛋白的荧光猝灭作用, 证明了这种荧光猝灭为静态猝灭机制, 计算了其结合常数和结合位点数, 从而得出了静电作用力为其主要的作用力.

动态膜电解制备四价铀Ⅰ.装置建立及性能测试

以钛涂钌铱网为阳极、钛网为阴极,建立了动态膜电解制备四价铀装置,测试了该装置的性能,比较了使用国产膜和进口膜时的电解性能,同时还探讨了制备过程中各参数的变化。研究结果表明:该装置能在电流密度高达120mA/cm2的情况下稳定运行;隔膜采用贝斯特Nepem-417膜或旭化成Aciplex-F4112膜,对电解结果无显著差异;对于500mL铀质量浓度为199g/L的料液,仅采用72cm2的钛涂钌铱网为阳极、钛网作为阴极,电解180min,四价铀产率可达到93.1%;电解前后阴极料液中肼浓度从0.52mol/L下降为0.30mol/L,酸浓度从2.20mol/L下降为0.70mol/L,槽压在3～3.6V内变动,阴极电解液温度低于45℃(室温28℃)。

丁苯橡胶中苯乙烯含量的测定

叙述了应用光学法和裂鲜色谱法对丁苯橡胶中苯乙烯含量的测定方法。

Spatial pattern analysis for quantification of landscape structure of Tadoba-Andhari Tiger Reserve,Central India

Landscape structure is often regarded as an important factor that governs the distribution and abundance of species. Therefore it is critical to understand the landscapes and their dynamics. Patterns of landscape elements strongly influence the ecological characteristics. This study was designed to document and map the current status of the tropi-cal dry deciduous forest of the Tadoba-Andhari Tiger Reserve （TATR）, Central India, （using IRS P6 LISS IV data） and to describe its landscape structure at three levels of organization viz. landscape, class, and patch. The study area was classified into 10 land cover classes that include 6 vegetation classes. The landscape structure was analyzed using FRAG-STATS using 12 set of indices. The TATR landscapes have a total of 2,307 patches with a mean patch size of 25.67 ha and patch density of 1.7 patches per km2. Amongst all land cover classes, mixed bamboo forest is dominant-it occupied maximum area （77.99%）-while riparian forest is least represented （0.32%）. Mixed forest has maximum number of patches among all vegetation classes. Results have shown that despite being dominant in the area, mixed bamboo forest has low patch density （0.25/100 ha）. Dominance of mixed bamboo forest is attributed to large patch sizes and not to the number of patches. This study has focussed on the approach of integrating satellite forest classification and forest inven-tory data for studying forest landscape patterns.

Monitoring Land Use and Infrastructure Changes in Industrial Complex Using Geo-Informatics Technology in Gujarat State, India

In the present study, detailed investigations have been carried out in Petroleum, Chemicals and Petrochemical Investment Region (PCPIR) area in Vygra and Bharuch Talukas in Bharuch district of Gujarat State. Indian Remote Sensing Satellite (IRS-P6) LISS-III, LISS-IV and CARTOSAT digital data covering PCPIR area in Bharuch district for the period of January & February of 2011, 2012 and 2013 was analyzed for land use/land cover mapping and monitoring the changes in land use. Various thematic land use/land cover maps were prepared and GIS database for various thematic layers have been generated using satellite and ground based information. The results indicate that the major land use in the PCPIR area is agriculture with crop lands ranging from 61 to 63 per cent of the total area. Crop land has decreased from 64.7% during 2011 to 62.7% during 2013 in the PCPIR region. Area under plantations in PCPIR area has also decreased from 5.5% during 2011 to 5.2% during 2012. The industrial area has increased from 6.0% to 7.6% of the total area of the PCPIR region. The total built-up area (industries & village area) has increased from 7.1% during 2011 to 8.7% during 2013. Tree plantations in the area of around 42 ha were carried out by GIDC during 2012 and 2013 to increase the green cover in the PCPIR area.

南京“5．7”^192Ir源放射事故患者的临床救治

目的 通过对南京＂5.7＂192Ir源放射事故患者的剂量估算、临床表现和医学处理的总结,探讨类似放射损伤的救治技术和发生、发展规律。方法 医护人员询问受照患者的详细病史,进行物理剂量和生物剂量估算,应用红外线热成像仪动态监测损伤部位的温度改变。进行综合分析,确定诊断和治疗方案。治疗上,全身和局部治疗并重,躯体治疗和心理治疗同步,采取综合措施。给予改善微循环、增强免疫功能、营养支持、抗感染、应用粒细胞集落刺激因子（G-CSF）及对症治疗等进行全身治疗;局部治疗包括镇痛、换药、清创及两次肌皮瓣移植术、脐带间充质干细胞（MSCs）输注、负压封闭引流（VSD）等。结果 患者全身生物剂量估算结果为1.51 Gy,物理估算右下肢皮肤最大剂量值约为4 100 Gy,该结果和临床表现一致;患者受照后约40 d平稳进入恢复期;经过378 d的精心救治,右下肢Ⅳ度急性放射性皮肤损伤伤口愈合,但仍存在活动障碍。结论 早期进行局部不均匀照射的生物剂量估算结果与临床诊断相符,结合系统的医学检查指导救治方案的制定;轻度骨髓型急性放射病病程分期不明显,预后较好;局部严重放射性损伤迁延不愈,肌皮瓣移植是治疗的关键,显微外科技术、VSD封闭式引流技术及MSCs输注是手术成功的关键;抗感染、营养治疗及心理疏导对患者全身及局部的康复起到了积极作用。

槲皮素锗的合成及表征

槲皮素在碱溶液中,与锗离子反应生成槲皮素锗并通过元素分析、紫外光谱、红外光谱和热差分析对槲皮素锗进行了表征。