AIM：To evaluate the intrinsic excitability of retinal ganglion cells（RGCs） in degenerated retinas.METHODS：The intrinsic excitability of various morphologically defined RGC types using a combination of patch-clamp ...AIM：To evaluate the intrinsic excitability of retinal ganglion cells（RGCs） in degenerated retinas.METHODS：The intrinsic excitability of various morphologically defined RGC types using a combination of patch-clamp recording and the Lucifer yellow tracer in retinal wholemount preparations harvested from Royal College of Surgeons（RCS） rats,a common retinitis pigmentosa（RP） model,in a relatively late stage of retinal degeneration（P90） were investigated.Several parameters of RGC morphologies and action potentials（APs） were measured and compared to those of non-dystrophic control rats,including dendritic stratification,dendritic field diameter,peak amplitude,half width,resting membrane potential,AP threshold,depolarization to threshold,and firing rates.RESULTS：Compared with non-dystrophic control RGCs,more depolarizations were required to reach the AP threshold in RCS RGCs with low spontaneous spike rates and in RCS OFF cells（especially A2o cells）,and RCS RGCs maintained their dendritic morphologies,resting membrane potentials and capabilities to generate APs.CONCLUSION：RGCs are relatively well preserved morphologically and functionally,and some cells are more susceptible to decreased excitability during retinal degeneration.These findings provide valuable considerations for optimizing RP therapeutic strategies.展开更多
IBS Pap er Per for manc e Group（以下简称I B S）是一家在制浆造纸领域全球闻名的工程公司,有着50多年行业和产品的经验,业务遍及世界各地。其核心业务是设计和制造高性能系统解决方案和优质的产品,提供集成系统的结合和创新系统解...IBS Pap er Per for manc e Group（以下简称I B S）是一家在制浆造纸领域全球闻名的工程公司,有着50多年行业和产品的经验,业务遍及世界各地。其核心业务是设计和制造高性能系统解决方案和优质的产品,提供集成系统的结合和创新系统解决方案。目前IBS拥有八个强势品牌,15家子公司,50位服务工程师,30位研发/设计工程师,20位应用工程师/造纸专家,650位雇员。展开更多
基金Supported by the National Basic Research Program of China(973 Program,No.2013CB967002)the Military Key Program(No.BWS13C015)the Chongqing International Cooperation Key Projects(No.CSTC2013GJHZ10004)
文摘AIM：To evaluate the intrinsic excitability of retinal ganglion cells（RGCs） in degenerated retinas.METHODS：The intrinsic excitability of various morphologically defined RGC types using a combination of patch-clamp recording and the Lucifer yellow tracer in retinal wholemount preparations harvested from Royal College of Surgeons（RCS） rats,a common retinitis pigmentosa（RP） model,in a relatively late stage of retinal degeneration（P90） were investigated.Several parameters of RGC morphologies and action potentials（APs） were measured and compared to those of non-dystrophic control rats,including dendritic stratification,dendritic field diameter,peak amplitude,half width,resting membrane potential,AP threshold,depolarization to threshold,and firing rates.RESULTS：Compared with non-dystrophic control RGCs,more depolarizations were required to reach the AP threshold in RCS RGCs with low spontaneous spike rates and in RCS OFF cells（especially A2o cells）,and RCS RGCs maintained their dendritic morphologies,resting membrane potentials and capabilities to generate APs.CONCLUSION：RGCs are relatively well preserved morphologically and functionally,and some cells are more susceptible to decreased excitability during retinal degeneration.These findings provide valuable considerations for optimizing RP therapeutic strategies.
文摘IBS Pap er Per for manc e Group（以下简称I B S）是一家在制浆造纸领域全球闻名的工程公司,有着50多年行业和产品的经验,业务遍及世界各地。其核心业务是设计和制造高性能系统解决方案和优质的产品,提供集成系统的结合和创新系统解决方案。目前IBS拥有八个强势品牌,15家子公司,50位服务工程师,30位研发/设计工程师,20位应用工程师/造纸专家,650位雇员。
