That Ho cells in rat liver express desmin was confirmed by immunohistochemical technique. Consequently, changes of desmin-positive cells, lysozyme-positive cells and fibronectin were further studied in experimental ci...That Ho cells in rat liver express desmin was confirmed by immunohistochemical technique. Consequently, changes of desmin-positive cells, lysozyme-positive cells and fibronectin were further studied in experimental cirrhosis of rat. It was found that desmln- positive cells, with the transitional feature between Ito cells and myofibroblasts or fibrobiasts under electron microscope, increased in number and expression of desmin in the necrotic areas as well as in the cellular fibrous septa, but decreased in number in the fibrous septa except those areas closed to the edges of the septa. These results suggested that Ito cells, myofibroblasts and fibroblasts might belong to the same cellular system and play an important role in the pathogenesis of cirrhosis. Meanwhile, it was also noted that changes of both fibronectin and lysozymepositive cells were correlated with those of desmin-positive cells. These provide evidence in vivo that flbronectin and Kupffer cells might exert certain effects on the migration and proliferation of Ito cells in liver cirrhosis.展开更多
Objective Evidences have shown that Ito cells were important in hepatic fibrosis and manycytokines were involved in the activation and regulation of ito cells from static - to dynamic - state. This programwas designed...Objective Evidences have shown that Ito cells were important in hepatic fibrosis and manycytokines were involved in the activation and regulation of ito cells from static - to dynamic - state. This programwas designed to observe the expression of TGF- α in ito cells. Methods The ito cells were successfully isolatedin vitro and the expression of TGF- α was measured by reverse - transcription polymerase chain reaction(RT- PCR). Results The TGF-α transcription significantly enhanced in hepatic fibrosis. The ratio of TGF-αto β- actin was 0.89±0.11 in normal rats ito cells (n=6), 3.72±0.25 in hepatic fibrosis rats ito cells (n=6)respectively (P<0.05). Conclusion TGF-α played an important role in activation and regulation of hepaticfibrosis.展开更多
The application of transparent conducting indium-tin-oxide （ITO） film as full front electrode replacing the conven- tional bus-bar metal electrode in III-V compound GalnP solar cell was proposed. A high-quality, non...The application of transparent conducting indium-tin-oxide （ITO） film as full front electrode replacing the conven- tional bus-bar metal electrode in III-V compound GalnP solar cell was proposed. A high-quality, non-rectifying contact between ITO and 10 nm N＋-GaAs contact layer was formed, which is benefiting from a high carrier concentration of the terrilium-doped N＋-GaAs layer, up to 2×10^19 cm^-3. A good device performance of the GalnP solar cell with the ITO electrode was observed. This result indicates a great potential of transparent conducting films in the future fabrication of larger area flexible III-V solar cell.展开更多
胞吐是细胞之间传递信号的主要方式之一,安培法监测细胞胞吐是研究细胞间通讯的基本方法.本研究采用ITO(Indium Tin Oxide)导电玻璃与紫外光刻技术构建了一种新型ITO微孔电化学传感器,用于细胞胞吐现象的监测并取得较好的效果.相比传统...胞吐是细胞之间传递信号的主要方式之一,安培法监测细胞胞吐是研究细胞间通讯的基本方法.本研究采用ITO(Indium Tin Oxide)导电玻璃与紫外光刻技术构建了一种新型ITO微孔电化学传感器,用于细胞胞吐现象的监测并取得较好的效果.相比传统方法,ITO微孔电化学传感器不仅可以取代传统的碳纤维微电极,而且可与荧光或化学发光等方法联用,运用多种手段同时对细胞的生理现象进行监测.展开更多
文摘That Ho cells in rat liver express desmin was confirmed by immunohistochemical technique. Consequently, changes of desmin-positive cells, lysozyme-positive cells and fibronectin were further studied in experimental cirrhosis of rat. It was found that desmln- positive cells, with the transitional feature between Ito cells and myofibroblasts or fibrobiasts under electron microscope, increased in number and expression of desmin in the necrotic areas as well as in the cellular fibrous septa, but decreased in number in the fibrous septa except those areas closed to the edges of the septa. These results suggested that Ito cells, myofibroblasts and fibroblasts might belong to the same cellular system and play an important role in the pathogenesis of cirrhosis. Meanwhile, it was also noted that changes of both fibronectin and lysozymepositive cells were correlated with those of desmin-positive cells. These provide evidence in vivo that flbronectin and Kupffer cells might exert certain effects on the migration and proliferation of Ito cells in liver cirrhosis.
文摘Objective Evidences have shown that Ito cells were important in hepatic fibrosis and manycytokines were involved in the activation and regulation of ito cells from static - to dynamic - state. This programwas designed to observe the expression of TGF- α in ito cells. Methods The ito cells were successfully isolatedin vitro and the expression of TGF- α was measured by reverse - transcription polymerase chain reaction(RT- PCR). Results The TGF-α transcription significantly enhanced in hepatic fibrosis. The ratio of TGF-αto β- actin was 0.89±0.11 in normal rats ito cells (n=6), 3.72±0.25 in hepatic fibrosis rats ito cells (n=6)respectively (P<0.05). Conclusion TGF-α played an important role in activation and regulation of hepaticfibrosis.
基金Project supported by the National Natural Science Foundation of China(Grant Nos.61534008,61376081,and 61404157)the Application Foundation of Suzhou,China(Grant No.SYG201437)
文摘The application of transparent conducting indium-tin-oxide （ITO） film as full front electrode replacing the conven- tional bus-bar metal electrode in III-V compound GalnP solar cell was proposed. A high-quality, non-rectifying contact between ITO and 10 nm N＋-GaAs contact layer was formed, which is benefiting from a high carrier concentration of the terrilium-doped N＋-GaAs layer, up to 2×10^19 cm^-3. A good device performance of the GalnP solar cell with the ITO electrode was observed. This result indicates a great potential of transparent conducting films in the future fabrication of larger area flexible III-V solar cell.
文摘胞吐是细胞之间传递信号的主要方式之一,安培法监测细胞胞吐是研究细胞间通讯的基本方法.本研究采用ITO(Indium Tin Oxide)导电玻璃与紫外光刻技术构建了一种新型ITO微孔电化学传感器,用于细胞胞吐现象的监测并取得较好的效果.相比传统方法,ITO微孔电化学传感器不仅可以取代传统的碳纤维微电极,而且可与荧光或化学发光等方法联用,运用多种手段同时对细胞的生理现象进行监测.
